CELENA® X

The CELENA® X High Content Imaging System is an integrated imaging system designed for rapid, high content image acquisition and analysis. Customizable imaging protocols, image-based and laser autofocusing modules, and a motorized XYZ stage simplify well plate imaging and slide scanning. The integrated CELENA® X Cell Analyzer software processes images and data for quantitative analysis. Analysis pipelines can be put together and reused to identify cellular or subcellular objects, process images for optimal data collection, and make various measurements. The CELENA® X is as flexible as it is powerful, with interchangeable objectives and filter cubes to accommodate a wide range of fixed and live cell imaging applications.

Fully Automated Plate And Slide Imaging

Automated vessel handling and scanning
Motorized XYZ stage, filter cube stage, and objective turret

Laser Autofocus

Rapid and reproducible focusing
Minimized phototoxicity and photobleaching

Live Cell Assay Support

Onstage incubation system for a variety of experiments in physiological and non-physiological conditions

Four Imaging Modes

Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging

Powerful, Easy-To-Use User Interface

Simple setup of imaging protocols
Seamless integration of imaging and data analysis processes

Customizable High Content Analysis

Create and customize image analysis projects
Quantitatively analyze multiple image-based phenotypes

Create Your Own Imaging And Analysis Workflows With The CELENA® X

Imaging

Raw images
Metadata

Image Analysis

Morphology of individual cells and organelles
Spatial distribution of targets
Multiple measurements per cell
Differentiation of multiple phenotypes

Data Analysis

Analyzed images
Result files
Graphs (coming soon)

Abundant Applications

The CELENA® X Cell Analyzer software is incredibly powerful and its ability to batch process makes it ideal for high content analysis. The powerful and flexible software allows for the creation and customization of workflows that can be used for the simplest fixed cell assays to more complicated, time-lapse live cell assays.

Cell counting

Cytotoxicity_BF

Transfection efficiency

Versatile And Customizable For Your Cell Imaging Needs

Vessel Holder Selection Guide

A collection of vessel holders are available to accommodate various flasks, dishes, plates, and slides.

Vessel Holder Selection Guide

Objective Selection Guide

Download our objective selection guide to see the Olympus objectives we recommend for the CELENA® X.

Objective Selection Guide

LED Filter Cube Selection Guide

Download our LED filter cube selection guide to find which filter cubes fit your needs the best.

LED Filter Cube Selection Guide

What Do Our Customers Have To Say?

Testimonials

(5.0)

Peter Davis

Great to use, inexpensive - watch out big microscopy companies...
The Celena-X is so easy to use, it has huge flexibility and it is oh so affordable making the 'big name brands' look like a rip off!

ATA Scientific

(5.0)

David Benz

Perfect combination of high content and high quality imaging.
The speed of image acquisition is outstanding and the images generated are of high quality. Together with the easy-to-use software, it is a great product.

Institut für Zellbiologie und Immunologie Stuttgart

Where The CELENA® X Has Been Cited

Citations

Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells.

2021. Shim MS, Nettesheim A, Dixon A, Liton PB. Proceedings of the National Academy of Sciences of the United States of America 118(13):e2021942118.

Cathepsin B Localizes in the Caveolae and Participates in the Proteolytic Cascade in Trabecular Meshwork Cells. Potential New Drug Target for the Treatment of Glaucoma.

2021. Nettesheim A, Shim MS, Dixon A, Raychaudhuri U, Gong H, Liton PB. Journal of Clinical Medicine 10(1):78.

Indirubin-3'-monoxime induces paraptosis in MDA-MB-231 breast cancer cells by transmitting Ca 2+ from endoplasmic reticulum to mitochondria.

2021. Dilshara MG, Neelaka Molagoda IM, Prasad Tharanga Jayasooriya RG, Choi YH, Park C, Kim GY. Archives of Biochemistry and Biophysics 698:108723.

Revascularization and limb salvage following critical limb ischemia by nanoceria-induced Ref-1/APE1-dependent angiogenesis.

2020. Park IS, Mahapatra C, Park JS, Dashnyam K, Kim JW, Ahn JC, Chung PS, Yoon DS, Mandakhbayar N, Singh RK, Lee JH, Leong KW, Kim HW. Biomaterials 242:119919.

Synthesis and Properties of CurNQ for the Theranostic Application in Ovarian Cancer Intervention.

2020. Freidus LG, Kumar P, Marimuthu T, Pradeep P, Pillay V, Choonara YE. Molecules (Basel, Switzerland) 25(19):4471.

Ordering Information

Cat #	Product	Qty
CX30000	CELENA® X High Content Imaging System	1 unit
I10530	CX Stage Top Incubator, Basic [Tokai]	1 set
I10531	CX Stage Top Incubator, Pro [Tokai]	1 set
I10500	Heating System for Multi-well Plates [Ibidi]	1 set
I10502	Gas Incubation System for CO₂ [Ibidi]	1 set
I10503	Gas Incubation System for CO₂/O₂ [Ibidi]	1 set
I10030	UPLFLN 4X, Olympus	1 unit
I10031	UPLFLN 10X2, Olympus	1 unit
I10036	LUCPLFLN 20X2, Olympus	1 unit
I10037	LUCPLFLN 40X2, Olympus	1 unit
I10038	UPLFLN 4XPH, Olympus	1 unit
I10039	UPLFLN 10X2PH, Olympus	1 unit
I10044	LUCPLFLN 20XPH2, Olympus	1 unit
I10045	LUCPLFLN 40XPH2, Olympus	1 unit
I10046	PLAPON 1.25X, Olympus	1 unit
I10047	PLAPON 2X, Olympus	1 unit
I10052	UPLXAPO 60XO, Olympus	1 unit
I10051	UPLXAPO 100XO, Olympus	1 unit
I10130	DAPI (Ex395/25, Em460/50)	1 unit
I10131	EGFP (Ex470/30, Em530/50)	1 unit
I10132	RFP (Ex530/40, Em605/55)	1 unit
I10133	mCherry (Ex580/25, Em645/75)	1 unit
I10134	ECFP (Ex436/20, Em480/40)	1 unit
I10135	EYFP (Ex500/20, Em535/30)	1 unit
I10136	DSRed (Ex530/40, Em620/60)	1 unit
I10137	Cy5 (Ex620/60, Em700/75)	1 unit
I10138	Cy7 (Ex710/75, Em810/90)	1 unit
I10139	Cy3/TRITC Long Pass (Ex530/40, Em570lp)	1 unit
I10140	GFP Long Pass (Ex470/40, Em500lp)	1 unit
I10141	Cy5 Long Pass (Ex620/60, Em665lp)	1 unit
I10142	Custom Filters	1 unit
I10410	Joystick	1 unit

Specifications

Supported labware	Slides, multi-well plates (6 to 1536 wells), petri dishes, culture flasks
Imaging modes	4-channel fluorescence, brightfield, phase contrast, color brightfield
Light source	High-power LED filter cubes with adjustable intensity (>50,000 hours per filter cube)
Filter cube stage	Motorized; 4 interchangeable fluorescence filter cubes and 1 brightfield filter cube
Available filters	DAPI, EGFP, RFP, mCherry, ECFP, EYFP, DSRed, Cy5, Cy7, Cy3/TRITC Long Pass, GFP Long Pass, Cy5 Long Pass, custom filters
Objective turret	Motorized; 5 interchangeable objectives
Compatible objectives	1.25-100X; Olympus, Zeiss, and Logos Biosystems objectives
Condenser	Motorized; basic or phase contrast Basic: 60 mm LWD condenser, 4 positions Phase contrast: 60 mm LWD condenser, 4 positions with 3 phase annuli
Camera	Monochrome: CMOS, 1.92 MP (optional) Color: CMOS, 1.92 MP
Image outputs	Monochrome: 16‐bit (12‐bit dynamic range) TIF, PNG, or JPG Color: 24-bit color TIF, PNG, or JPG Movies: MP4
Autofocus method	Image-based autofocus (optional) Laser autofocus
Stage	Motorized X/Y-stage (120 mm x 80 mm); motorized Z-stage (10 mm)
Stage control	CELENA^® X Explorer (optional) Joystick
Computer	External PC
Monitor	27” 4K UHD monitor
Software	User interface: CELENA^® X Explorer Analysis: CELENA^® X Cell Analyzer
Power	100-240 VAC, 250 W, 50/60 Hz
Dimensions	Main body: 39 x 46 x 50 cm (15.4 x 18.1 x 19.7 in) Controller: 17 x 30 x 23 cm (6.7 x 11.8 x 9.1 in)
Weight	Main body: 33 kg (72.8 lbs) Controller: 7 kg (15.4 lbs)

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