TISSUE CLEARING
DeepLabel™ Antibody Staining Kit
for Cleared Tissues

DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging.

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ANTIBODY LABELING
DeepLabel™ Antibody Staining Kit  C33001

DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues (compatible with CLARITY, PACT, iDISCO, CUBIC, etc).

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Compatible with
multiple clearing methods
Simple protocol
Vibrant imaging at
subcellular resolution
Site-specific labeling
Homogenous antibody distribution
2.6 X greater signal-to-background
The effect of DeepLabel™ on anti-Collagen IV penetration into mouse brain tissues
Without DeepLabel
With DeepLabel
Adult mouse brain tissue stained with anti-Collagen IV (red) and imaged with a confocal microscope.
Adult mouse brain stained using DeepLabel with anti-β-Amyloid (yellow), anti-Iba1 (red), and anti-SMA (purple).
Whole adult Drosophila brain stained using DeepLabel with anti-GFP (green) and anti-TH (red).
Whole adult mouse brain stained using DeepLabel with anti-TH (red).
IMAGING
X-CLARITY™ Mounting Solution  C13101  |  C13102  |  C13107

X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS) modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.

APP NOTE
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RI = 1.46 at 25°C
Minimizes photobleaching
Preserves fluorescence
Human cerebral cortex (1 mm)
Before ETC
PFA-fixed (1 week)
After ETC
After RI matching
Courtesy of Hyung Jin Choi, PhD
Seoul National University College of Medicine
Volumetric images of auditory neurons in a zebra finch brain (3 mm)
Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution. Auditory neurons of a zebra finch were labeled with GFP bilaterally via injection of a viral vector encoding GFP, stained with propidium iodide (PI) to visualize sample structure, and imaged with a light sheet microscope.
Courtesy of Hyung Jin Choi, PhD
Seoul National University College of Medicine
What Do Our Customers Have To Say?
Testimonials
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Where The DEEPLABEL™ S Has Been Cited
Citations

Multiplex immunolabeling and imaging of functionally essential kidney structures in X-CLARITY-cleared tissue.

2019. Mochama P, Tyshynsky R, Sanders MA. Microscopy and Microanalysis 25(Supplement S2): 1260-1261.

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Resources
Downloads
DeepLabel™ Antibody Staining Kit
Flyer

2022-04-07 | 458.10 KB

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X-CLARITY™ Systems and Reagents for Tissue Clearing
Brochure

2022-04-07 | 962KB

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Logos Biosystems Products
Brochure

2022-04-07 | 2.14 MB

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The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
Product literature

2018-11-26 | 1.25 MB

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2022-04-21 | 548.40 KB

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Ordering Information
Cat #ProductQty
C33001DeepLabel™ Antibody Staining Kit
– DeepLabel™ Solution A (1 x 25 mL)
– DeepLabel™ Solution B (2 x 25 mL)
– DeepLabel™ Washing Buffer (1 x 250 mL)
1 kit
C33002DeepLabel™ Solution A1 x 25 mL
C33003DeepLabel™ Solution B2 x 25 mL
C33004DeepLabel™ Washing Buffer1 x 250 mL
C13101X-CLARITY™ Mounting Solution1 x 25 mL
C13102X-CLARITY™ Mounting Solution Value Pack10 x 25 mL
C13107X-CLARITY™ Mounting Solution Bulk Pack20 x 25 mL