DeepLabel™ antibody staining kit for cleared tissues with box and reagent bottles for immunolabeling

TISSUE CLEARING

DeepLabel™ Antibody Staining Kit
for Cleared Tissues

DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging.

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ANTIBODY LABELING

DeepLabel™ Antibody Staining Kit  C33001

DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues (compatible with CLARITY, PACT, iDISCO, CUBIC, etc).

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DeepLabel™ antibody labeling kit bottles and packaging for tissue clearing and immunostaining
Compatible with
multiple clearing methods
Simple protocol
Vibrant imaging at
subcellular resolution
Site-specific labeling
Homogenous antibody distribution
2.6 X greater signal-to-background

The effect of DeepLabel™ on anti-Collagen IV penetration into mouse brain tissues

Without DeepLabel
Automated fluorescence microscopy image of adult mouse brain tissue stained with anti-Collagen IV without DeepLabel kit showing limited antibody penetration and weak red signal
With DeepLabel
Automated fluorescence microscopy image of adult mouse brain tissue stained with anti-Collagen IV without DeepLabel kit showing limited antibody penetration and weak red signal
Adult mouse brain tissue stained with anti-Collagen IV (red) and imaged with a confocal microscope.
DeepLabel antibody staining of adult mouse brain with β-Amyloid (yellow), Iba1 (red), and SMA (purple) for multiplex fluorescence imaging
Adult mouse brain stained using DeepLabel with anti-β-Amyloid (yellow), anti-Iba1 (red), and anti-SMA (purple).
DeepLabel fluorescent labeling of adult Drosophila brain with GFP (green) and TH (red) for high-resolution neuroimaging
Whole adult Drosophila brain stained using DeepLabel with anti-GFP (green) and anti-TH (red).
DeepLabel whole mouse brain antibody staining with TH (red) for 3D fluorescence tissue imaging
Whole adult mouse brain stained using DeepLabel with anti-TH (red).
IMAGING
X-CLARITY™ Mounting Solution  C13101  |  C13102  |  C13107

X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS) modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.

Read X-CLARITY™ Application Note
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DeepLabel™ antibody staining kit bottles for 3D imaging of cleared tissue samples
RI = 1.46 at 25°C
Minimizes photobleaching
Preserves fluorescence
Human cerebral cortex (1 mm)
Human cerebral cortex sample before tissue clearing showing opaque structure for baseline comparison
Before ETC
PFA-fixed (1 week)
Human cerebral cortex sample after ETC clearing showing partial transparency for imaging preparation
After ETC
Human cerebral cortex sample after RI matching showing enhanced transparency for high-resolution microscopy
After RI matching
Courtesy of Hyung Jin Choi, PhD
Seoul National University College of Medicine
Volumetric images of auditory neurons in a zebra finch brain (3 mm)
Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution. Auditory neurons of a zebra finch were labeled with GFP bilaterally via injection of a viral vector encoding GFP, stained with propidium iodide (PI) to visualize sample structure, and imaged with a light sheet microscope.
Courtesy of Hyung Jin Choi, PhD
Seoul National University College of Medicine

Resources

Downloads

DeepLabel™ Antibody Staining Kit
Flyer

2022-04-07 | 458.10 KB

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X-CLARITY™ Systems and Reagents for Tissue Clearing
Brochure

2022-04-07 | 962KB

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Logos Biosystems Products
Brochure

2022-04-07 | 2.14 MB

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The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
Product literature

2018-11-26 | 1.25 MB

Download

2022-04-21 | 548.40 KB

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Ordering Information
Cat # Product Qty
C33001 DeepLabel™ Antibody Staining Kit
– DeepLabel™ Solution A (1 x 25 mL)
– DeepLabel™ Solution B (2 x 25 mL)
– DeepLabel™ Washing Buffer (1 x 250 mL)
1 kit
C33002 DeepLabel™ Solution A 1 x 25 mL
C33003 DeepLabel™ Solution B 2 x 25 mL
C33004 DeepLabel™ Washing Buffer 1 x 250 mL
C13101 X-CLARITY™ Mounting Solution 1 x 25 mL
C13102 X-CLARITY™ Mounting Solution Value Pack 10 x 25 mL
C13107 X-CLARITY™ Mounting Solution Bulk Pack 20 x 25 mL