LUNA-FL™ Dual Fluorescence Cell Counter

Equipped with dual fluorescence and brightfield optics, the LUNA-FL™ is a powerhouse. The LUNA-FL™ can assess cell counts, viability, and GFP transfection efficiency without being limited by cell type or size.

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With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

Two Counters In One
The LUNA-FL™ can count in both brightfield and fluorescence mode, making it our most versatile counter to date.
Compatible With Most Cells
Advanced imaging software and GFP and RFP fluorescence optics sensitively detect fluorescence stained cells.
Gfp Transfection Analysis
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
Maximum 30 Seconds
Fluorescence: 30 s = 3 images captured & analyzed;Brightfield: 10 s = 1 image captured & analyzed
The Software That Changes Everything.
The Lowest Cost. The Greatest Convenience.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
What Do Our Customers Have To Say?
Moying Li

Great performance, would recommend for other researchers!
Easy to use, saves us time and offers good quality. The consultant Dr Eberhardt was also professional and patient to teach us before use.

University Heidelberg

Mahmood Ali

Great instrument, I like using it. Really helpful.
The device is easy to use, I mainly use it before cell seeding or to check for my culture's viability and survival. The results are reproducible and the device provides fast results. The accuracy seems to be great as well.


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Where The LUNA-FL™ Has Been Cited

Brain Cancer Cell-derived Exosomes Protect Scopolamine-Induced Death of SH-SY5Y Neuron Cells.

2021. Lee M. International Journal of Health Sciences and Research 10.36838/v3i1.8.

The Type and Source of Reactive Oxygen Species Influences the Outcome of Oxidative Stress in Cultured Cells.

2021. Goffart S, Tikkanen P, Michell C, Wilson T, Pohjoismäki JLOLO. Cells 10(5):1075.

Immune Memory in Mild COVID-19 Patients and Unexposed Donors Reveals Persistent T Cell Responses After SARS-CoV-2 Infection.

2021. Ansari A, Arya R, Sachan S, Jha SN, Kalia A, Lall A, Sette A, Grifoni A, Weiskopf D, Coshic P, Sharma A, Gupta N. Frontiers in Immunology 12:636768.

The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System.

2021. Fujibayashi K, Mima J. Frontiers in Cell and Developmental Biology 9:628910.

Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids.

2021. Metzger W, Rösch B, Sossong D, Bubel M, Pohlemann T. Cell Biology International 10.1002/cbin.11618.

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2022-04-07 | 2.12 MB

Logos Biosystems Products

2022-04-07 | 2.14 MB

UNA-FL™: GFP Transfection Assay
Quick Start Guide

2018-04-16 | 110 KB

Quick Start Guide

2018-04-16 | 284 KB

Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images
Product literature

2018-04-16 | 1.7 MB

Application of a non-hazardous vital dye for cell counting with automated cell counters
Product literature

2018-04-13 | 906 KB


2018-04-16 | 476 KB

Cell DB
HL-60 (high density)
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human peripheral blood
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
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Ordering Information
Cat #ProductQty
L20001LUNA-FL™ Automated Fluorescence Cell Counter1 unit
L12005PhotonSlide™, 50 Slides1 box
L12006PhotonSlide™, 500 Slides10 boxes
L12007PhotonSlide™, 1000 Slides20 boxes
L12001LUNA™ Cell Counting Slides, 50 Slides1 box
L12002LUNA™ Cell Counting Slides, 500 Slides10 boxes
L12003LUNA™ Cell Counting Slides, 1000 Slides20 boxes
L12011LUNA™ Reusable Slide1 unit
L12012LUNA™ Reusable Slides2 units
L12014LUNA™ Reusable Slide Coverslips10 units
T13001Trypan Blue Stain, 0.4%2 X 1 mL
L13002Erythrosin B Stain2 x 1 mL
F23001Acridine Orange/Propidium Iodide Stain2 x 0.5 mL
F23002Acridine Orange Stain2 x 0.5 mL
F23003Propidium Iodide Stain2 x 0.5 mL
F23202Yeast Viability Kit 1
– Propidium Iodide Stain for Yeast (1 x 0.5 mL)
– Fluorescein Diacetate Stain (1 x 0.5 mL)
– Cell Dilution Buffer (1 x 20 mL)
– Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
1 kit
F23212Cell Dilution Buffer5 x 20 mL
F23102LUNA™ Fluorescence Calibration Beads1 x 0.5 mL
B13101LUNA™ Standard Beads2 x 1 mL
F24003LUNA-FL™ IQ/OQ Protocol1 copy
P10001LUNA™ Printer I1 unit
P12001LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls)3 x 2 rolls
Sample Volume10 μl
Cell Counting Time30 sec
Cell Concentration Range5×104 – 1×107 cells/mL
Cell Size RangeDetectable Range: 1 – 90 μm
Optimal Range: 5 – 60 μm
Excitation wavelength470 ± 20 nm
Emission wavelength530 ± 25 nm, 600 nm (LP)
Light SourceLED
Image Resolution5 MP
LCD Display800 x 480 pixels
Dimensions (W×D×H)22 × 21 × 9 cm (8.6 × 8.3 × 3.5 in)
Weight1.8 kg (4  lb)  *without the AC adapter and power cord