Tissue clearing for high-resolution 3d imaging

Tissue clearing system X-CLARITY with hydrogel and electrophoretic units for 3D imaging sample preparation | Logos Biosystems

X-CLARITY™

Systems & Reagents for Tissue Clearing

DeepLabel antibody staining kit for tissue clearing with bottles and reagents displayed for 3D imaging | Logos Biosystems

DeepLabel™

Antibody Staining Kit for Cleared Tissues

Sectioning is time. We cut the time.

Tissue clearing is essential for non-destructive, three-dimensional (3D) imaging of biological samples. Combined with labeling techniques, tissue clearing allows for the visualization of cellular structures in their physiological context. X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. X-CLARITY™ is based on CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging / Immunostaining /in situ-hybridization-compatible Tissue hYdrogel), a tissue clearing method developed by the Deisseroth lab at Stanford University. The new DeepLabel™ Antibody Staining Kit accelerates immunolabeling of cleared tissues for a broad range of applications.

Simple, Rapid, and Reproducible Tissue Processing

Simple protocol and ready-to-use reagents Adaptable for various tissue types and applications Long-term preservation of endogenous FP Efficient and homogenous antibody labeling

Accelerate Your 3d Imaging Workflow

X-CLARITY hydrogel solution kit and tissue clearing system for electrophoretic and passive clearing of biological samples
STEP 1. Tissue Clearing

A fixed tissue sample is incubated in the X-CLARITY™ Hydrogel Solution Kit to allow hydrogel monomers to diffuse uniformly throughout the sample and then placed in the X-CLARITY™ Polymerization System. Monomers polymerize in the anaerobic environment, linking the biomolecules to a sturdy hydrogel network, preserving molecular information and structural integrity. The tissue-hydrogel hybrid is cleared in the X-CLARITY™ Tissue Clearing System II using the ready-to-use Electrophoretic Tissue Clearing Solution. Lipids are extracted actively through electrophoresis or passively, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping.

X-CLARITY™ HYDROGEL SOLUTION KIT   >
X-CLARITY™ POLYMERIZATION SYSTEM   >
X-CLARITY™ TISSUE CLEARING SYSTEM II   >
ELECTROPHORETIC TISSUE CLEARING SOLUTION   >
DeepLabel antibody staining kit bottles for enhanced penetration and labeling in cleared tissue samples
STEP 2. Antibody Labeling

The transparent sample is then labled with antibodies using the DeepLabel™ Antibody Staining Kit, which enhances antibody penetration into clarified tissues. Optimized for clarified tissue samples, DeepLabel™ can be used with tissues cleared with various clearing methods such as passive or active CLARITY, iDISCO, 3DISCO, uDISCO, Visikol, or CUBIC.

DEEPLABEL ANTIBODY™ STAINING KIT   >
X-CLARITY mounting solution bottle for refractive index matching to increase optical transparency in cleared tissues
STEP 3. RI Matching for Imaging

Prior to imaging, the tissue-hydrogel hybrid is placed in a refractive index (RI) matching solution in order to homogenize the environment within the tissue to the solution. This reduces light scatter, which in turn increases optical transparency and consequently increases image quality and imaging depth.

X-CLARITY™ MOUNTING SOLUTION   >
     

What Scientists Say About Tissue Clearing

Researcher portrait associated with testimonial on using X-CLARITY tissue clearing system for brain imaging

“[The X-CLARITY] has actually been fundamental to the Data Brain Project because without being able to clear tissues of the lipids, we wouldn’t be able to image deep into brains. So we wouldn’t be able to extract that structural information that is so important to understand the function of mammalian brains. It’s been a huge advantage and a great leap forward in imaging.”

Arti Ahluwalia, PhD
Centro E. Piaggio | University of Pisa
Researcher portrait associated with testimonial on using X-CLARITY tissue clearing system for brain imaging

“Thanks to X-CLARITY, we now have access to the threedimensions of the whole heart organ.”

Gabriel Bidaux, PhD
INSERM