The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophoretic tissue clearing simple, rapid, and efficient.
The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream.
The X-CLARITY™ Hydrogel Solution Kit is a pre-tested hydrogel solution for uniform and consistent tissue-hydrogel hybridization. The kit is made up of X-CLARITY™ Hydrogel Solution, an acrylamide-based solution used to create polyacrylamide, and X-CLARITY™ Polymerization Initiator, a thermal free radical initiator also known as VA-044.
The X-CLARITY™ Polymerization System is a standalone, automated system developed to simplify tissue-hydrogel hybridization, a crucial step for optimal tissue clearing. Multiple samples can be placed in multi-well plates or conical tubes for rapid and efficient high-throughput sample processing. Users can control polymerization by adjusting vacuum strength, temperature, and a timer through a simple touchscreen interface.
The X-CLARITY™ Tissue Clearing System II is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. Clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology. Users can set tissue clearing conditions through a simple and intuitive touchscreen interface. In ETC (electrophoretic tissue clearing) mode, platinum-plated electrodes generate an electric field to accelerate the removal of lipids from tissues in a highly efficient manner. A built-in temperature control system actively cools and heats buffer to maintain consistent buffer temperatures during clearing. Buffer is constantly circulated to ensure consistent buffering capacity, temperature control, and elimination of tissue clearing byproducts. This advanced system ensures efficient, rapid, and consistent tissue clearing.
Electrophoretic Tissue Clearing Solution is a premixed SDS-based buffer optimized for use with the X-CLARITY™ Tissue Clearing System II.
For you if you want to accelerate your research!
Positive facts: X-CLARITY system is easy to use, devoted to aqueous-based method for organ clearing. It helps clearing several organs at once and in a short period of time. Works well on a lot of different mouse organs or even pieces of primates and humans organs. Logos Biosystems after Sales are fast and always find a compromise as a solution to our rare issues. Minus: expensive systems in comparison to passive clearing methods. For you if you want to accelerate your research!
INSERM UMR 1060
Great results, easy to use!
This product was effective at clearing my tissue samples. It cleared whole brains, hearts, stomach, kidneys, livers, etc. The user manual was easy to read and easy to follow. There is little attention and maintenance required while the system in running.
Influenza causes alterations to oligodendrocyte-specific transcripts and the myelin lipidome in the adult mouse central nervous system.
2021. Louie A, Kim J, Soto-Diaz K, Dibaeinia P, Koito H, Sinha S, Nowak R, Das A, Steelman A. Research Squre https://doi.org/10.21203/rs.3.rs-310861/v1.
Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models.
2021. Chourrout M, Rositi H, Ong E, Hubert V, Paccalet A, Foucault L, Autret A, Fayard B, Olivier C, Bolbos R, Peyrin F, Crola-da-Silva C, Meyronet D, Raineteau O, Elleaume H, Brun E, Chauveau F, Wiart M. bioRxiv 2021.03.24.436852.
Novel insights into the electrophysiology of murine cardiac macrophages: relevance of voltage-gated potassium channels.
2021. Simon-Chica A, Fernández MC, Wülfers EM, Lother A, Hilgendorf I, Seemann G, Ravens U, Kohl P, Schneider-Warme F. Cardiovascular Research cvab126.
3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice.
2020. Ded L, Hwang JY, Miki K, Shi HF, Chung JJ. Elife 9:e62043.
Maximizing lentiviral vector gene transfer in the CNS.
2020. Humbel M, Ramosaj M, Zimmer V, Regio S, Aeby L, Moser S, Boizot A, Sipion M, Rey M, Déglon N. Gene Therapy 10.1038/s41434-020-0172-6.
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|C20001||X-CLARITY™ Polymerization System||1 unit|
|C20002||X-CLARITY™ Heat Block for 6 x 50 mL tubes||1 unit|
|C20003||X-CLARITY™ Heat Block for flat-bottom plates||1 unit|
|C1310X||X-CLARITY™ Hydrogel Solution Kit|
– X-CLARITY™ Hydrogel Solution (1 x 1 L)
– X-CLARITY™ Polymerization Initiator (1 x 2.5 g)
|C30001||X-CLARITY™ Tissue Clearing System II||1 unit|
|C12010||Holder for 36 Mouse Brain Slices, 1.5 Φ||1 set|
|C12020||Holder for 36 Mouse Brain Slices, 0.6 Φ||1 set|
|C12011||Holder for 6 Slices, 1.5 Φ||1 set|
|C12021||Holder for 6 Slices, 0.6 Φ||1 set|
|C12012||Holder for 1 Sample, 1.5 Φ||1 set|
|C12022||Holder for 1 Sample, 0.6 Φ||1 set|
|C12013||Holder for 6 Mouse Brains, 1.5 Φ||1 set|
|C12023||Holder for 6 Mouse Brains, 0.6 Φ||1 set|
|C12014||Holder for 48 Samples, 1.5 Φ||1 set|
|C12024||Holder for 48 Samples, 0.6 Φ||1 set|
|C12015||Holder for 192 Samples, 1.5 Φ||1 set|
|C12025||Holder for 192 Samples, 0.6 Φ||1 set|
|C12001||Tissue Container (Whole Mouse Brain), 20 units||1 box|
|C12002||Container Holder for 1 Tissue Container||1 unit|
|C12004||Mouse Brain Slice Holder (Chamber Height: 4 mm)||1 unit|
|C12007||Whole Rat Brain Holder||1 unit|
|C13001||Electrophoretic Tissue Clearing Solution||12 x 1 L|
|C13101||X-CLARITY™ Mounting Solution||1 x 25 mL|
|C13102||X-CLARITY™ Mounting Solution Value Pack||10 x 25 mL|
|C13107||X-CLARITY™ Mounting Solution Bulk Pack||20 x 25 mL|
|C33001||DeepLabel™ Antibody Staining Kit|
– DeepLabel™ Solution A (1 x 25 mL)
– DeepLabel™ Solution B (2 x 25 mL)
– DeepLabel™ Washing Buffer (1 x 250 mL)
|Display||5” TFT LCD|
|Temperature Range||RT – 60 °C|
|Temperature Accuracy||±0.3 °C|
|Vacuum Range||-90 – 0 kPa|
|Power Consumption||312 W|
|Applicable Power||AC 100-240 V, 50/60 Hz|
|Dimensions (W x D x H)||Exterior: 332 x 430 x 222 mm|
Interior: 307 x 137 x 140 mm
X-CLARITY™ ETC Chamber
|Instrument type||Electrophoretic chamber|
|External dimensions (W x D x H)||176 mm x 128 mm x 154 mm|
|Internal dimensions (W x D x H)||57 mm x 30 mm x 93 mm|
X-CLARITY™ ETC Control Tower
|Instrument type||Power supply, temperature control,|
and buffer circulation device
|User interface||5 “ TFT LCD touchscreen|
|Power supply modes||Constant current or constant voltage|
|Current & voltage range||0.2-1.5 A, 5-70 V|
|Temperature range||30-60 ° C|
|Pump speed range||50-200 rpm|
|Electrical requirements||AC 100-240 V, 50/60 Hz|
|Power consumption||500 W (including the X-CLARITY™ ETC Chamber)|
|Dimensions (W x D x H)||205 mm x 430 mm x 370 mm|