CELENA® X High Content Imaging System

Automated high content image acquisition and analysis for drug discovery and cell biology

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The CELENA® X High Content Imaging System is an integrated imaging system designed for rapid, high content image acquisition and analysis. Customizable imaging protocols, image-based and laser autofocusing modules, and a motorized XYZ stage simplify well plate imaging and slide scanning. The integrated CELENA® X Cell Analyzer software processes images and data for quantitative analysis. Analysis pipelines can be put together and reused to identify cellular or subcellular objects, process images for optimal data collection, and make various measurements. The CELENA® X is as flexible as it is powerful, with interchangeable objectives and filter cubes to accommodate a wide range of fixed and live cell imaging applications.

Fully Automated Plate And Slide Imaging
Automated vessel handling and scanning
Motorized XYZ stage, filter cube stage, and objective turret
Laser Autofocus
Rapid and reproducible focusing
Minimized phototoxicity and photobleaching
Live Cell Assay Support
Onstage incubation system for a variety of experiments in physiological and non-physiological conditions
Four Imaging Modes
Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging
Powerful, Easy-To-Use User Interface
Simple setup of imaging protocols
Seamless integration of imaging and data analysis processes
Customizable High Content Analysis
Create and customize image analysis projects
Quantitatively analyze multiple image-based phenotypes
Create Your Own Imaging And Analysis Workflows With The CELENA® X
Raw images
Image Analysis
Morphology of individual cells and organelles
Spatial distribution of targets
Multiple measurements per cell
Differentiation of multiple phenotypes
Data Analysis
Analyzed images
Result files
Graphs (coming soon)
Abundant Applications
The CELENA® X Cell Analyzer software is incredibly powerful and its ability to batch process makes it ideal for high content analysis. The powerful and flexible software allows for the creation and customization of workflows that can be used for the simplest fixed cell assays to more complicated, time-lapse live cell assays.
Versatile And Customizable For Your Cell Imaging Needs
Vessel Holder Selection Guide
A collection of vessel holders are available to accommodate various flasks, dishes, plates, and slides.
Vessel Holder Selection Guide
Objective Selection Guide
Download our objective selection guide to see the Olympus objectives we recommend for the CELENA® X.
Objective Selection Guide
LED Filter Cube Selection Guide
Download our LED filter cube selection guide to find which filter cubes fit your needs the best.
LED Filter Cube Selection Guide
What Do Our Customers Have To Say?
Peter Davis

Great to use, inexpensive - watch out big microscopy companies...
The Celena-X is so easy to use, it has huge flexibility and it is oh so affordable making the 'big name brands' look like a rip off!

ATA Scientific

David Benz

Perfect combination of high content and high quality imaging.
The speed of image acquisition is outstanding and the images generated are of high quality. Together with the easy-to-use software, it is a great product.

Institut für Zellbiologie und Immunologie Stuttgart

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Where The CELENA® X Has Been Cited

Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells.

2021. Shim MS, Nettesheim A, Dixon A, Liton PB. Proceedings of the National Academy of Sciences of the United States of America 118(13):e2021942118.

Cathepsin B Localizes in the Caveolae and Participates in the Proteolytic Cascade in Trabecular Meshwork Cells. Potential New Drug Target for the Treatment of Glaucoma.

2021. Nettesheim A, Shim MS, Dixon A, Raychaudhuri U, Gong H, Liton PB. Journal of Clinical Medicine 10(1):78.

Indirubin-3'-monoxime induces paraptosis in MDA-MB-231 breast cancer cells by transmitting Ca 2+ from endoplasmic reticulum to mitochondria.

2021. Dilshara MG, Neelaka Molagoda IM, Prasad Tharanga Jayasooriya RG, Choi YH, Park C, Kim GY. Archives of Biochemistry and Biophysics 698:108723.

Revascularization and limb salvage following critical limb ischemia by nanoceria-induced Ref-1/APE1-dependent angiogenesis.

2020. Park IS, Mahapatra C, Park JS, Dashnyam K, Kim JW, Ahn JC, Chung PS, Yoon DS, Mandakhbayar N, Singh RK, Lee JH, Leong KW, Kim HW. Biomaterials 242:119919.

Synthesis and Properties of CurNQ for the Theranostic Application in Ovarian Cancer Intervention.

2020. Freidus LG, Kumar P, Marimuthu T, Pradeep P, Pillay V, Choonara YE. Molecules (Basel, Switzerland) 25(19):4471.

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2022-04-07 | 2 MB

CELENA®X vessel holder dimension
Selection Guide

2021-07-01 | 282.34 KB

CELENA®X High Content Imaging System
Installation Guide

2021-06-18 | 665.56 KB

Quick Start Guide

2019-10-30 | 901.13 KB

Ordering Information
Cat #ProductQty
CX30000CELENA® X High Content Imaging System1 unit
I10530CX Stage Top Incubator, Basic [Tokai]1 set
I10531CX Stage Top Incubator, Pro [Tokai]1 set
I10500Heating System for Multi-well Plates [Ibidi]1 set
I10502Gas Incubation System for CO2 [Ibidi]1 set
I10503Gas Incubation System for CO2/O2 [Ibidi]1 set
I10030UPLFLN 4X, Olympus1 unit
I10031UPLFLN 10X2, Olympus1 unit
I10034LUCPLFLN 20X, Olympus1 unit
I10035LUCPLFLN 40X, Olympus1 unit
I10038UPLFLN 4XPH, Olympus1 unit
I10039UPLFLN 10X2PH, Olympus1 unit
I10042LUCPLFLN 20XPH, Olympus1 unit
I10043LUCPLFLN 40XPH, Olympus1 unit
I10046PLAPON 1.25X, Olympus1 unit
I10047PLAPON 2X, Olympus1 unit
I10052UPLXAPO 60XO, Olympus1 unit
I10051UPLXAPO 100XO, Olympus1 unit
I10130DAPI (Ex395/25, Em460/50)1 unit
I10131EGFP (Ex470/30, Em530/50)1 unit
I10132RFP (Ex530/40, Em605/55)1 unit
I10133mCherry (Ex580/25, Em645/75)1 unit
I10134ECFP (Ex436/20, Em480/40)1 unit
I10135EYFP (Ex500/20, Em535/30)1 unit
I10136DSRed (Ex530/40, Em620/60)1 unit
I10137Cy5 (Ex620/60, Em700/75)1 unit
I10138Cy7 (Ex710/75, Em810/90)1 unit
I10139Cy3/TRITC Long Pass (Ex530/40, Em570lp)1 unit
I10140GFP Long Pass (Ex470/40, Em500lp)1 unit
I10141Cy5 Long Pass (Ex620/60, Em665lp)1 unit
I10142Custom Filters1 unit
I10410Joystick1 unit


Supported labwareSlides, multi-well plates (6 to 1536 wells), petri dishes, culture flasks
Imaging modes4-channel fluorescence, brightfield, phase contrast, color brightfield
Light sourceHigh-power LED filter cubes with adjustable intensity (>50,000 hours per filter cube)
Filter cube stageMotorized; 4 interchangeable fluorescence filter cubes and 1 brightfield filter cube
Available filtersDAPI, EGFP, RFP, mCherry, ECFP, EYFP, DSRed, Cy5, Cy7, Cy3/TRITC Long Pass, GFP Long Pass, Cy5 Long Pass, custom filters
Objective turretMotorized; 5 interchangeable objectives
Compatible objectives1.25-100X; Olympus, Zeiss, and Logos Biosystems objectives
CondenserMotorized; basic or phase contrast
Basic: 60 mm LWD condenser, 4 positions
Phase contrast: 60 mm LWD condenser, 4 positions with 3 phase annuli
CameraMonochrome: CMOS, 1.92 MP
(optional) Color: CMOS, 1.92 MP
Image outputsMonochrome: 16‐bit (12‐bit dynamic range) TIF, PNG, or JPG
Color: 24-bit color TIF, PNG, or JPG
Movies: MP4
Autofocus methodImage-based autofocus
(optional) Laser autofocus
StageMotorized X/Y-stage (120 mm x 80 mm); motorized Z-stage (10 mm)
Stage controlCELENA® X Explorer
(optional) Joystick
ComputerExternal PC
Monitor27” 4K UHD monitor
SoftwareUser interface: CELENA® X Explorer
Analysis: CELENA® X Cell Analyzer
Power100-240 VAC, 250 W, 50/60 Hz
DimensionsMain body: 39 x 46 x 50 cm (15.4 x 18.1 x 19.7 in)
Controller: 17 x 30 x 23 cm (6.7 x 11.8 x 9.1 in)
WeightMain body: 33 kg (72.8 lbs)
Controller: 7 kg (15.4 lbs)