Cell Counting  > Consumables & Accessories  > Cell Counting Reagents
Acridine Orange/Propidium Iodide Stain

Acridine Orange/Propidium Iodide Stain is a cell viability dye that causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red. Acridine Orange/Propidium Iodide Stain can be used to assess cell viability with the automated fluorescence cell counters of the LUNA™ family.

Acridine orange permeates viable cells and binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red. Propidium iodide binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20-30 fold and causes the cell to fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.

Compatible Instruments
LUNA-FL™ Automated Cell Counter
LUNA-STEM™ Automated Cell Counter
LUNA-II YF™ Automated Yeast Cell Counter
LUNA-FX7™ Automated Cell Counter


AppearanceOrange-red liquid
Cell permeabilityMembrane permeable (acridine orange)
Membrane impermeant (propidium iodide)
Excitation/emission500/526nm (acridine orange)
533/617nm (propidium iodide)


Ordering Information

Cat #DescriptionQty
F23001Acridine Orange/Propidium Iodide Stain2 x 0.5 mL
F23011Acridine Orange/Propidium Iodide Stain, Sterile-filtered2 x 0.5 mL