Cell Counting  > Consumables & Accessories  > Cell Counting Reagents
Yeast Viability Kit 1

Yeast Viability Kit 1 is used to analyze the cell concentration and viability of yeast samples with the automated fluorescence cell counters of the LUNA™ family.

Fluorescein Diacetate Stain and Propidium Iodide Stain for Yeast are used to assess cell viability. Viable cells hydrolyze fluorescein diacetate into the green fluorescent compound fluorescein, causing viable cells to fluoresce green. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes binding to the nucleus of nonviable cells and causing them to fluoresce red.

Fluorescence Signal Enhancer 1 is an inhibitor of ATP-dependent fluorescein efflux. By blocking fluorescein from being pumped out of the cell, Fluorescence Signal Enhancer 1 lowers background and increases signal intensity, thereby enhancing the fluorescein signalto-noise ratio. Fluorescence Signal Enhancer 1 is most effective with metabolically active cells in the log growth phase. Cell Dilution Buffer is for washing cells and diluting cell suspensions.

Compatible Instruments
LUNA-FL™ Automated Cell Counter
LUNA-II YF™ Automated Yeast Cell Counter
LUNA-FX7™ Automated Cell Counter


Fluorescein Diacetate Stain
Molecular formulaC24H16O7
Molecular weight416.38 g/mol
AppearanceClear liquid
Cell permeabilityMembrane permeant
Excitation/emission494/521 nm (when converted to fluorescein)
Quantity1 x 0.5 mL
Storage-20°C in the dark


Propidium Iodide for Yeast
Molecular formulaC27H34I2N4
Molecular weight668.39 g/mol
AppearanceClear liquid
Cell permeabilityMembrane permeant
Excitation/emission493/636 nm (in aqueous solution)
533/617 nm (when bound to nucleic acids)
Quantity1 x 0.5 mL
Storage4°C in the dark


Fluorescence Signal Enhancer 1
AppearanceClear liquid
Quantity1 x 0.5 mL
Storage-20°C in the dark


Cell Dilution Buffer
AppearanceClear liquid
Quantity1 x 20 mL
StorageRoom temperature


Ordering Information

Cat #DescriptionQty
F23202Yeast Viability Kit 11 Kit