MICROBIAL CELL COUNTER
LUNA-II YF™ Automated Yeast Cell Counter

Yeast strains used in food and drink production can be notoriously clumpy.The LUNA-II YF™ has an autofocusing cell counting and declustering algorithm that counts individual yeast cells in just 15 seconds.

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As is well-known in brewing and winemaking industries, the quantity of yeast pitched to initiate fermentation directly affects the flavor, aroma, and quality of the final product. Although yeast can be pitched by weight or volume, such methods are inexact and highly variable. The ASBC method using methylene blue method is more accurate but time consuming and subjective. Use the LUNA-II YF™ to get accurate and reproducible yeast cell counts in as little as 15 seconds. This little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

Accurate Yeast Cell Counts
The LUNA-II YF™ provides cell count and viability data with the precision and consistency of a flow cytometer.
15 Seconds To Results
Using dual fluorescence optics, an autofocusing liquid lens, and advance software, the LUNA-II YF™ produces data in just 15 seconds.
Yeast-Optimized Analysis
Unlike flow cytometers, the LUNA-II YF™ has advanced declusteringcapabilities, and can count even the clumpiest cells.
Verified With Multiple Strains
We’ve independently tested 30+ brewing yeast products on the LUNA-II YF™.Visit the cell DB for a partial list.
The Software That Changes Everything.
The Lowest Cost. The Greatest Convenience.
Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
What Do Our Customers Have To Say?
Testimonials
(5.0)
Alireza Hassani Najafabadi

Great results and value.
I like this product. The machine is so good and accurate. I always check the results with manually counting and the results are consistent.

University of Michigan

(5.0)
Le Dinh De

Great product. I am an equipment manager. I am very pleased with this product to check and count enamel in beer. Fast, accurate and complete parameters are the most basic factors. It is very easy to use. After sales care service is very good. Product worth the money.

Sabeco (Saigon Beer-Alcohol-Beverage Corporation)

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Where The LUNA-II-YF™ Has Been Cited
Citations
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Resources
Downloads
LUNA-II YF™
flyer

2022-04-07 | 421.36 KB

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Logos Biosystems Products
Brochure

2022-04-07 | 2.14 MB

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LUNA-II YF™
Quick Start Guide

2021-06-18 | 614.45 KB

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Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images
Product literature

2018-04-16 | 1.7 MB

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Cell DB
Fermentis
Saflager S-23 Lager Yeast
Yeast cells stained with FDA/PI
Default Protocol: FDA-PI
l Dilution factor: 1.18
l Min. fluorescent object size: 1 µm
l Max. fluorescent object size: 60 µm
l Green fluorescence exposure: 4
l Red fluorescence exposure: 6
Fermentis
Safale WB-06 Wheat Beer
Yeast cells stained with FDA/PI
Default Protocol: FDA-PI
l Dilution factor: 1.18
l Min. fluorescent object size: 1 µm
l Max. fluorescent object size: 60 µm
l Green fluorescence exposure: 4
l Red fluorescence exposure: 6
Lallemand
Munich Wheat Beer
Yeast cells stained with AO/PI
Default Protocol: AO-PI
l Dilution factor: 1.11
l Min. fluorescent object size: 1 µm
l Max. fluorescent object size: 60 µm
l Green fluorescence exposure: 7
l Red fluorescence exposure: 3
Lallemand
Nottingham Ale
Yeast cells stained with AO/PI
Default Protocol: AO-PI
l Dilution factor: 1.11
l Min. fluorescent object size: 1 µm
l Max. fluorescent object size: 60 µm
l Green fluorescence exposure: 7
l Red fluorescence exposure: 3
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Ordering Information
Cat #ProductQty
L50001LUNA-II YF™ Automated Yeast Cell Counter1 unit
L12005PhotonSlide™, 50 Slides1 box
L12006PhotonSlide™, 500 Slides10 boxes
L12007PhotonSlide™, 1000 Slides20 boxes
L12011LUNA™ Reusable Slide1 unit
L12012LUNA™ Reusable Slides2 units
L12014LUNA™ Reusable Slide Coverslips10 units
F23001Acridine Orange/Propidium Iodide Stain2 x 0.5 mL
F23002Acridine Orange Stain2 x 0.5 mL
F23003Propidium Iodide Stain2 x 0.5 mL
F53002Cell Dilution Buffer II5 x 20 mL
F23202Yeast Viability Kit 1
– Propidium Iodide Stain for Yeast (1 x 0.5 mL)
– Fluorescein Diacetate Stain (1 x 0.5 mL)
– Cell Dilution Buffer (1 x 20 mL)
– Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
1 kit
F23102LUNA™ Fluorescence Calibration Beads2 x 1 mL
L54003LUNA-II YF™ IQ/OQ Protocol1 copy
P10001LUNA™ Printer I1 unit
P12001LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls)3 x 2 rolls
Specifications
Instrument TypeBenchtop cell counter
Dimensions (W x D x H)16 x 18 x 28 cm (6.3 x 7.0 x 11.0 in)
Weight1.8 kg (3.9 lb)
Cell Concentration Range5 x 104 – 1 x 107 cells/mL
Cell Diameter Range1 – 60 µm (optimal range: 1-30 µm)
Cell Viability Range0 – 100%
Image TypeTIFF, 1.1 MP
Processing Time*10** (manual focusing) or 15** (autofocusing) seconds at ~1 x 106 cell/mL

 

*Processing time may vary according to cell type and concentration.
**This is the minimum processing time for each focusing option at the specified concentration of Saccharomyces cerevisiae.”