X-CLARITY tissue clearing system with electrophoretic chamber and cooling unit on lab bench for 3D imaging sample preparation | Logos Biosystems

TISSUE CLEARING

X-CLARITY™ Systèmes et Réactifs pour la
Transparisation des Tissus

Logos Biosystems a développé la gamme X-CLARITY™, incluant des systèmes et de réactifs prêts à l'emploi, afin de rendre la transparisation par électrophorèse des tissus simple, rapide et efficace.

Demander une démonstration gratuite

Grâce au X-CLARITYTM, chaque étape du processus de transparisation peut être normalisée, simplifiée et accélérée. Dans la méthode CLARITY, les tissus préservés sont intégrés dans des matrices d'hydrogel, et les lipides sont extraits par électrophorèse pour produire un hybride tissu-hydrogel stable. Le tissu optiquement transparent est prêt pour l'immunomarquage et peut ensuite être imagé. La cytoarchitecture native reste intacte, les protéines fluorescentes endogènes sont préservées pour une imagerie par fluorescence de qualité.

01. HYDROGEL INFUSION

X-CLARITY Hydrogel Solution Kit bottle and reagent tube for tissue clearing experiments
X-CLARITY™
Hydrogel Solution Kit
C1310X

02. TISSUE-HYDROGEL HYBRIDIZATION

Diagram showing stabilized hydrogel matrix structure formed inside liver tissue during polymerization
X-CLARITY Polymerization System device with red cover for tissue clearing workflow
X-CLARITY™
Polymerization System
C20001

03. LIPID REMOVAL

Diagram showing electrophoretic tissue clearing process with electric field applied to liver tissue sample
Automated X-CLARITY Tissue Clearing System II for efficient and reproducible tissue clearing experiments
X-CLARITY™
Tissue Clearing System II
C30001
Electrophoretic Tissue Clearing Solution reagent bottle for X-CLARITY tissue clearing system
Electrophoretic
Tissue Clearing Solution
C13001
           

01. Hydrogel Infusion

X-CLARITY™ Hydrogel Solution Kit  C1310X

Le kit de solution d'hydrogel X-CLARITY™ est une solution d'hydrogel pré-testée pour une hybridation tissu-hydrogel uniforme et cohérente. Le kit est composé d'une solution d'hydrogel X-CLARITY™, solution à base d'acrylamide utilisée pour créer du polyacrylamide, et de l'initiateur de polymerisation X-CLARITY™, un initiateur thermique de radicaux libres également connu sous le nom de VA-044.

View Hydrogel Solution Kit

02. Tissue-Hydrogel Hybridization

X-CLARITY™ Polymerization System  C20001

Le système de polymérisation X-CLARITY™ est un système autonome et automatisé développé pour simplifier l'hybridation tissu-hydrogel, une étape cruciale pour la transparisaton optimale des tissus. Plusieurs échantillons peuvent être placés dans des plaques multipuits ou des tubes coniques pour un traitement à haut débit, rapide et efficace. L'interface à écran tactile permet aux utilisateurs de régler le vide, la température et une minuterie pour contrôler le processus de polymérisation.

Download Software Update Manual
X-CLARITY Tissue Clearing System device with red lid and front control panel for electrophoretic clearing
Fast tissue clearing icon for accelerated turnaround
Haut débit
(jusqu'à 768 échantillons/analyse)
Reproducibility icon indicating standardized results
Compatible avec divers
supports
Easy-to-use icon for simplified operation
Contrôle constant du vide et de la température
Safety/quality icon for reliable tissue processing
Polymérisation rapide et constante
Accessoires
X-CLARITY Heat Block accessory designed to hold six 50 mL conical tubes for tissue clearing
X-CLARITY™ Heat Block for 6 x 50 mL tubes
Catalog #: C20002
Quantity : 1 unité
Holds six 50 mL conical tubes
X-CLARITY Heat Block accessory for flat-bottom plates used in tissue clearing workflows
X-CLARITY™ Heat Block for flat-bottom plates
Catalog #: C20003
Quantity : 1 unité
Holds one flat-bottom plate
           

03. Lipid Removal

X-CLARITY™ Tissue Clearing System II  C30001

Le système de transparisation des tissus X-CLARITY™ Tissue Clearing System II est une solution tout-en-un, facile à utiliser, pour la transparisation des tissus par électrophorèse. La conception unique de cet appareil lui permet d'éliminer efficacement les lipides des tissus tout en préservant l'intégrité de l'échantillon. Il est incroyablement 8 fois plus rapide que la technologie originale pour transparisiser un cerveau entier de souris, en seulement 6 heures. Grâce à l'interface de l'écran tactile, les utilisateurs sont en mesure de définir les conditions de transparisation des tissus. Une électrode en platine génère un champ électrique qui accélère l'élimination des lipides des tissus pendant l'ETC (transparisation électrophorétique des tissus). Afin de maintenir le tampon à une température constante pendant la clarification, un système intégré de contrôle de la température refroidit ou réchauffe activement le tampon. Le tampon circule en permanence afin de maintenir un pouvoir tampon constant, de contrôler la température et d'éliminer tout sous-produit de la purification des tissus. Il s'agit d'un système avancé qui garantit une clarification rapide, efficace et constante des tissus.

Read X-CLARITY™ Application Note
Sample Holder Guide
X-CLARITY Tissue Clearing System II electrophoretic device with cooling unit and controller for high-resolution tissue clearing
Contrôle précis de la température
           
X-CLARITY system icon showing active buffer cooling and heating capacity for precise tissue clearing temperature control
Refroidissement actif du tampon et capacité de chauffage
X-CLARITY tissue clearing icon with sensitive accurate temperature sensor for stable clearing process
Capteur de température sensible et précis
Champ électrique uniforme
X-CLARITY electrophoretic tissue clearing platinum plated electrodes icon ensuring uniform electric field
Électrodes en platine
Constant current constant voltage CV CC icon for X-CLARITY tissue clearing stable clearing performance
Mode courant constant ou tension constante
Compatible avec de nombreux types et tailles de tissus
X-CLARITY icon for active and passive tissue clearing modes compatible with multiple tissue types
Mode actif et passif de transparisation
Sample holder icon showing various sizes available for X-CLARITY tissue clearing applications
Supports de différentes tailles
Configuration conviviale
Touchscreen interface icon for X-CLARITY tissue clearing system easy operation
Interface simple par écran tactile
Ready-to-use clearing solution reagent bottle icon for X-CLARITY tissue clearing workflow
Solution de transparisation prête à l'emploi
           

Electrophoretic Tissue Clearing Solution  C13001

La solution de transparisation des tissus par électrophorèse est un tampon prémélangé à base de SDS optimisé pour être utilisé avec le X-CLARITY™ Tissue Clearing System II.

View Electrophoretic Clearing Solution
Electrophoretic Tissue Clearing Solution bottles for X-CLARITY tissue clearing system optimized SDS buffer
Cerveau entier de souris Thy1-YFP
Whole Thy1-YFP mouse brain before and after tissue clearing with X-CLARITY system showing preserved fluorescent signal
Le signal Thy1-YFP est préservé et vibrant après la transparisation des tissus avec les système X-CLARITY™ et ses réactifs.
Cortex cérébral humain (1 mm)
Human cerebral cortex slice before ETC PFA fixation for tissue clearing
Avant ETC PFA-fixe
(1 semaine)
Human cerebral cortex slice after electrophoretic tissue clearing with X-CLARITY system
Après ETC
Human cerebral cortex slice after refractive index RI matching for transparency improvement
Après appariement IR
Courtesy of Hyung Jin Choi, PhD Seoul National University College of Medicine

Marquage et Imagerie

Accélérez le marquage des anticorps avec le TOUT NOUVEAU kit de marquage d'anticorps DeepLabel™.
Discover DeepLabel™
DeepLabel antibody labeling kit for tissue clearing and imaging, Logos Biosystems solution for fast and efficient immunostaining

Why Scientists Love X-CLARITY™ System

Témoignages

(5.0)
Gabriel Bidaux

For you if you want to accelerate your research!
Positive facts: X-CLARITY system is easy to use, devoted to aqueous-based method for organ clearing. It helps clearing several organs at once and in a short period of time. Works well on a lot of different mouse organs or even pieces of primates and humans organs. Logos Biosystems after Sales are fast and always find a compromise as a solution to our rare issues. Minus: expensive systems in comparison to passive clearing methods. For you if you want to accelerate your research!

INSERM UMR 1060

(5.0)
Tori N

Great results, easy to use!
This product was effective at clearing my tissue samples. It cleared whole brains, hearts, stomach, kidneys, livers, etc. The user manual was easy to read and easy to follow. There is little attention and maintenance required while the system in running.

Clemson University

Read Peer Reviews

OÙ LE X-CLARITY™ A ÉTÉ CITÉ

Citations

Influenza causes alterations to oligodendrocyte-specific transcripts and the myelin lipidome in the adult mouse central nervous system.

2021. Louie A, Kim J, Soto-Diaz K, Dibaeinia P, Koito H, Sinha S, Nowak R, Das A, Steelman A. Research Squre https://doi.org/10.21203/rs.3.rs-310861/v1.

Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models.

2021. Chourrout M, Rositi H, Ong E, Hubert V, Paccalet A, Foucault L, Autret A, Fayard B, Olivier C, Bolbos R, Peyrin F, Crola-da-Silva C, Meyronet D, Raineteau O, Elleaume H, Brun E, Chauveau F, Wiart M. bioRxiv 2021.03.24.436852.

Novel insights into the electrophysiology of murine cardiac macrophages: relevance of voltage-gated potassium channels.

2021. Simon-Chica A, Fernández MC, Wülfers EM, Lother A, Hilgendorf I, Seemann G, Ravens U, Kohl P, Schneider-Warme F. Cardiovascular Research cvab126.

3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice.

2020. Ded L, Hwang JY, Miki K, Shi HF, Chung JJ. Elife 9:e62043.

Maximizing lentiviral vector gene transfer in the CNS.

2020. Humbel M, Ramosaj M, Zimmer V, Regio S, Aeby L, Moser S, Boizot A, Sipion M, Rey M, Déglon N. Gene Therapy 10.1038/s41434-020-0172-6.

View Cited Works

Ressources

Téléchargements

DeepLabel™ Antibody Staining Kit
Flyer

2022-04-07 | 458.10 KB

Download
X-CLARITY™ Systems and Reagents for Tissue Clearing
Brochure

2022-04-07 | 962KB

Download
Logos Biosystems Products
Brochure

2022-04-07 | 2.14 MB

Download
The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
Affiche

2018-11-26 | 1.25 MB

Download
X-CLARITY™ Systems and Reagents for Tissue Clearing
Flyer

2018-05-08 | 553 KB

Download
X-CLARITY™ Tissue Clearing System
Guide de démarrage rapide

2018-05-08 | 668.54 KB

Download
X-CLARITY™ Tissue Clearing System II
Guide d’installation

2018-05-08 | 841.04 KB

Download
ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
Documentation produit

2018-04-17 | 2.6 MB

Download
An automated and high-throughput polymerization solution for downstream tissue clearing: the X-CLARITY™ Polymerization System
Documentation produit

2018-04-17 | 1.4 MB

Download

2022-04-21 | 548.40 KB

Download
Galerie d’images
X-CLARITY™ cleared whole mouse brain sample on grid background for high-resolution 3D tissue imaging
Mouse brain | X-CLARITY™
X-CLARITY™ tissue clearing of mouse brain showing optical transparency and structural preservation
Mouse brain | X-CLARITY™
X-CLARITY™ cleared mouse brain hemisphere prepared for detailed microscopy and analysis
Mouse brain hemisphere | X-CLARITY™
X-CLARITY™ tissue cleared 2 mm mouse brain slices demonstrating uniform transparency
Mouse brain - 2 mm slices | X-CLARITY™
X-CLARITY™ cleared mouse brain slices (1, 2, 3 mm) comparison before and after tissue clearing
Mouse brain - 1,2,3 mm slices | X-CLARITY™
X-CLARITY™ cleared mouse brain slice with fluorescent labeling from Korea Brain Research Institute
Mouse brain slice | X-CLARITY™
Courtesy of Youngshik Choe, PhD | Korea Brain Research Institute
Explore Entire Cell Image
Informations pour commander
Cat # Product Qty
C20001 X-CLARITY™ Polymerization System 1 unit
C20002 X-CLARITY™ Heat Block for 6 x 50 mL tubes 1 unit
C20003 X-CLARITY™ Heat Block for flat-bottom plates 1 unit
C1310X X-CLARITY™ Hydrogel Solution Kit
– X-CLARITY™ Hydrogel Solution (1 x 1 L)
– X-CLARITY™ Polymerization Initiator (1 x 2.5 g)
1 kit
C30001 X-CLARITY™ Tissue Clearing System II 1 unit
C12010 Holder for 36 Mouse Brain Slices, 1.5 Φ 1 set
C12020 Holder for 36 Mouse Brain Slices, 0.6 Φ 1 set
C12011 Holder for 6 Slices, 1.5 Φ 1 set
C12021 Holder for 6 Slices, 0.6 Φ 1 set
C12012 Holder for 1 Sample, 1.5 Φ 1 set
C12022 Holder for 1 Sample, 0.6 Φ 1 set
C12013 Holder for 6 Mouse Brains, 1.5 Φ 1 set
C12023 Holder for 6 Mouse Brains, 0.6 Φ 1 set
C12014 Holder for 48 Samples, 1.5 Φ 1 set
C12024 Holder for 48 Samples, 0.6 Φ 1 set
C12015 Holder for 192 Samples, 1.5 Φ 1 set
C12025 Holder for 192 Samples, 0.6 Φ 1 set
C12001 Tissue Container (Whole Mouse Brain), 20 units 1 box
C12002 Container Holder for 1 Tissue Container 1 unit
C12004 Mouse Brain Slice Holder (Chamber Height: 4 mm) 1 unit
C12007 Whole Rat Brain Holder 1 unit
C13001 Electrophoretic Tissue Clearing Solution 12 x 1 L
C13101 X-CLARITY™ Mounting Solution 1 x 25 mL
C13102 X-CLARITY™ Mounting Solution Value Pack 10 x 25 mL
C13107 X-CLARITY™ Mounting Solution Bulk Pack 20 x 25 mL
C33001 DeepLabel™ Antibody Staining Kit
– DeepLabel™ Solution A (1 x 25 mL)
– DeepLabel™ Solution B (2 x 25 mL)
– DeepLabel™ Washing Buffer (1 x 250 mL)
1 kit
Caractéristiques

 

X-CLARITY™ Polymerization System

 

Display 5”  TFT LCD
Temperature Range RT – 60 °C
Temperature Accuracy ±0.3 °C
Vacuum Range -90 – 0 kPa
Power Consumption 312 W
Applicable Power AC 100-240 V, 50/60 Hz
Dimensions (W x D x H) Exterior: 332 x 430 x 222 mm
Interior: 307 x 137 x 140 mm
Weight 28 kg

 

X-CLARITY™ ETC Chamber

 

Instrument type Electrophoretic chamber
External dimensions (W x D x H) 176 mm x 128 mm x 154 mm
Internal dimensions (W x D x H) 57 mm x 30 mm x 93 mm
Weight 2.8 kg

 

X-CLARITY™ ETC Control Tower

 

Instrument type Power supply, temperature control,
and buffer circulation device
User interface 5 “ TFT LCD touchscreen
Power supply modes Constant current or constant voltage
Current & voltage range 0.2-1.5 A, 5-70 V
Temperature range 30-60 ° C
Pump speed range 50-200 rpm
Electrical requirements AC 100-240 V, 50/60 Hz
Power consumption 500 W (including the X-CLARITY™ ETC Chamber)
Dimensions (W x D x H) 205 mm x 430 mm x 370 mm
Weight 20 kg