Propidium Iodide Stain


Propidium Iodide Stain is a fluorescent vital dye that binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20-30 fold and causes the cell to fluoresce red.

Propidium Iodide Stain can be used with Acridine Orange Stain (F23002) to assess cell viability with the automated fluorescence cell counters of the LUNA™ family. Viable nucleated cells will fluoresce green and nonviable nucleated cells will fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.

Product Description
Molecular formula C27H34I2N4
Molecular weight 668.39 g/mol
Appearance Clear liquid
Cell permeability Membrane permeant
Excitation/emission 493/636 nm (in aqueous solution)
533/617 nm (when bound to nucleic acids)
Quantity 2 x 0.5 mL
Storage 4 °C in the dark

This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
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