Wound healing analysis based on image segmentation
#wound healing assay #scratch test #collective cell migration #time lapse imaging #live cell imagin #high content imaging #high content analysis #HCAdownload pdf
Figure 1 shows one field-of-view at each time point, demonstrating wound closure over time. Evidence of cell migration is seen as early as 2 hours post-wound creation and the cell-free space steadily decreases over time as the cells move to close the space completely. Image segmentation allowed quantification of the surface area occupied by cells (Figure 2).
- Figure 1. Image analysis of BAEC cells showing wound closure over time. Red borders illustrate the separation between background and the areas covered by intact cells. The white boxes outline the initial wound site.
- Figure 2. Quantification of in vitro wound healing in BAEC cell images deduced from surface area occupied at the start and end of the experiment. N = 6 wells, 10 fields-of-view per well
Confluency analysis from brightfield images
This tutorial demonstrates how to quantify confluence from a batch of brightfield images in an objective and reproducible way using CELENA® X Cell Analyzer.