Evaluating transfection efficiency using the CELENA®X
#transfection efficiency #hoechst #cell counting #GFP expression #GFP-positive #high content imaging #high content analysis #HCAdownload pdf
- Table 1. Description of the pipeline used to assess transfection efficiency with CELENA® X Cell Analyzer
- Figure 1. Image analysis of GFP expression in HeLa cells 24 h post-transfection. Higher plasmid concentrations correlated with increased numbers of GFP-positive cells (green). Blue lines indicate control Hoechst-stained nuclei (blue) and magenta lines outline GFP-positive cells.
- Figure 2. Quantification of transfection efficiency. The number of GFP-positive cells were compared to the number of Hoechst-stained nuclei. Transfection efficiency improved with increasing concentrations of the GFP-expressing pCG-HttQ103 plasmid.