The LUNA-STEM™ takes adipose-derived stem cell and SVF samples to count live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.
When using tissue-derived cells such as stem cells or the cells from the adipose stromal vascular fraction (SVF), knowing cell concentration and viability is crucial to ensure and control the quality of downstream procedures. Tissue-derived samples are often contaminated with red blood cells and noncellular debris, making cell counting a time-consuming, subjective, and error-prone process. The LUNA-STEM™ is equipped with dual fluorescence and brightfield optics to distinguish live and dead nucleated cells from non-nucleated cells. Samples are stained with nucleic acid dyes to determine the concentration and viability of mononucleated cells. Non-nucleated cells are distinguished from debris and also counted. In as little as 30 seconds, the LUNA-STEM™ reports total cell concentration, nucleated cell concentration, non-nucleated cell concentration, cell viability, and average cell size. A high-resolution image with live cells labeled with green circles and dead cells with red circles can be viewed to confirm data visually.
Consistent and accurate day after day!The LUNA-STEM Counter is very quick, effective, and accurate. We use the LUNA-STEM Counter on a daily basis and have run into no troubles for 2 years now. In addition we are a consumer of the LUNA-STEM Slides and AOPI Fluorescent dye, both of which are very inexpensive and extremely obtainable - with help from outstanding customer service. Definitely worth the money.
Best cell counting. Reliable and easy to use.We use it to count mononucleated cells. Very useful and easy to use. Perfect for our daily use.
Hospital de la Santa Creu i Sant Pau
Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue.
2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.
Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation.
2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.
Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials
2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.
Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia.
2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.
The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging.
2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.
| Cat # | Product | Qty |
|---|---|---|
| L30001 | LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF | 1 unit |
| L12005 | PhotonSlide™, 50 Slides | 1 box |
| L12006 | PhotonSlide™, 500 Slides | 10 boxes |
| L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
| L12011 | LUNA™ Reusable Slide | 1 unit |
| L12012 | LUNA™ Reusable Slides | 2 units |
| L12014 | LUNA™ Reusable Slide Coverslips | 10 units |
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
| F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
| F23212 | Cell Dilution Buffer | 5 x 20 mL |
| F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
| L34003 | LUNA-STEM™ IQ/OQ Protocol | 1 copy |
| P10001 | LUNA™ Printer I | 1 unit |
| P12001 | LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls) | 3 x 2 rolls |
| Sample Volume | 10 μl |
| Cell Counting Time | 30-60 sec (depending on sample conditions) |
| Cell Concentration Range | 5×104 – 1×107 cells/mL (optimal range) |
| Cell Size Range | Detectable Range: 1 – 90 μm Optimal Range: 5 – 60 μm |
| Excitation wavelength | 470 ± 25 nm |
| Emission wavelength | 530 ± 25 nm, 600 nm (LP) |
| Light Source | LED |
| Image Resolution | 5 MP |
| LCD Display | 7 inches (800 x 480 pixels) |
| Dimensions (W×D×H) | 22 × 21 × 9 cm (8.6 × 8.3 × 3.5 in) |
| Weight | 1.8 kg (4 lb) *without the AC adapter |
