• X-CLARITY Systems and Reagents for Tissue Clearing
  • TISSUE CLEARING

    X-CLARITY™ Systems & Reagents for Tissue Clearing

    The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophoretic tissue clearing simple, rapid, and efficient.

TISSUE CLEARING FOR 3D IMAGING

The X-CLARITY™ systems and reagents standardize, simplify, and accelerate each step of the tissue clearing process. With the CLARITY method, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescence proteins are preserved for robust fluorescence imaging downstream.
  • 01HYDROGEL INFUSION
    LogosBiosystems X-CLARITY Hydrogel Solution Kit C1310X_list
    X-CLARITY™
    Hydrogel Solution Kit
    C1310X
  • 02TISSUE-HYDROGEL HYBRIDIZATION
    LogosBiosystems X-CLARITY Polymerization System_list
    X-CLARITY™
    Polymerization System
    C20001
  • 03LIPID REMOVAL
    LogosBiosystems X-CLARITY Tissue Clearing System II_list
    X-CLARITY™
    Tissue Clearing System II
    C30001
    LogosBiosystems Electrophoretic Tissue Clearing Solution_list
    Electrophoretic
    Tissue Clearing Solution
    C13001
01

HYDROGEL INFUSION

  • X-CLARITY™ Hydrogel Solution Kit C1310X
    The X-CLARITY™ Hydrogel Solution Kit is a pre-tested hydrogel solution for uniform and consistent tissue-hydrogel hybridization. The kit is made up of X-CLARITY™ Hydrogel Solution, an acrylamide-based solution used to create polyacrylamide, and X-CLARITY™ Polymerization Initiator, a thermal free radical initiator also known as VA-044.
  • X-CLARITY Hydrogel Solution Kit
HYDROGEL SOLUTION KIT
    Cat # Product Qty MSDS Product Insert
    C1310X X-CLARITY™ Hydrogel Solution Kit

    1 box

    FileAction
    C1310X X-CLARITY™ Hydrogel Solution Kit_MSDS.zipDownload 
    C1310X X-CLARITY™ Hydrogel Solution Kit VX1904-01.pdfDownload 
    C13103 X-CLARITY™ Hydrogel Solution

    1 L

    FileAction
    [C13103] X-CLARITY Polymerization HS_rev2.pdfDownload 
    C13103 X-CLARITY™ Hydrogel Solution VX1904-01.pdfDownload 
    C13104 X-CLARITY™ Polymerization Initiator

    2.5 g

    FileAction
    [C13104] X-CLARITY Polymerization PI_rev3.pdfDownload 
    C13104 X-CLARITY™ Polymerization Initiator VX1904-01.pdfDownload 
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    02

    TISSUE-HYDROGEL HYBRIDIZATION

    • X-CLARITY™ Polymerization System C20001
      The X-CLARITY™ Polymerization System is a standalone, automated system developed to simplify tissue-hydrogel hybridization, a crucial step for optimal tissue clearing. Multiple samples can be placed in multi-well plates or conical tubes for rapid and efficient high-throughput sample processing. Users can control polymerization by adjusting vacuum strength, temperature, and a timer through a simple touchscreen interface.
      App Note
    • X-CLARITY Polymerization System
    • LogosBiosystems X-CLARITY Polymerization System High throughput
      High throughput
      (up to 768 samples/run)
    • LogosBiosystems X-CLARITY Polymerization System Compatible with various vessels
      Compatible with various
      vessels
    • LogosBiosystems X-CLARITY Polymerization System Steady vacuum & temperature control
      Steady vacuum &
      temperature control
    • X-CLARITY Polymerization System Rapid and consistent polymerization
      Rapid and consistent
      polymerization
    Accessories
      • X-CLARITY™ Heat Block for 6 x 50 mL tubes
      • X-CLARITY™ Heat Block for 6 x 50 mL tubes

        Catalog #: C20002
        Quantity : 1 unit
        Holds six 50 mL conical tubes

      • X-CLARITY™ Heat Block for flat-bottom plates
      • X-CLARITY™ Heat Block for flat-bottom plates

        Catalog #: C20003
        Quantity : 1 unit
        Holds one flat-bottom plate

    03

    LIPID REMOVAL

    • X-CLARITY™ Tissue Clearing System II C30001
      The X-CLARITY™ Tissue Clearing System II is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. Clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology. Users can set tissue clearing conditions through a simple and intuitive touchscreen interface. In ETC (electrophoretic tissue clearing) mode, platinum-plated electrodes generate an electric field to accelerate the removal of lipids from tissues in a highly efficient manner. A built-in temperature control system actively cools and heats buffer to maintain consistent buffer temperatures during clearing. Buffer is constantly circulated to ensure consistent buffering capacity, temperature control, and elimination of tissue clearing byproducts. This advanced system ensures efficient, rapid, and consistent tissue clearing.
      APP NOTE SAMPLE HOLDERS
    • X-CLARITY Tissue Clearing System II
    • Precise temperature control
      • X-CLARITY Tissue Clearing System II Precise temperature control Active buffer cooling and heating capacity
        Active buffer cooling and
        heating capacity
      • X-CLARITY Tissue Clearing System II Precise temperature control Sensitive and accurate temperature sensor
        Sensitive and accurate
        temperature sensor
    • Uniform electric field
      • X-CLARITY Tissue Clearing System II Uniform electric field Platinum plated electrodes
        Platinum plated electrodes
      • X-CLARITY Tissue Clearing System II Uniform electric field Constant current and constant voltage modes
        Constant current and
        constant voltage modes
    • Compatible with multiple tissue types and sizes
      • X-CLARITY Tissue Clearing System II Compatible with multiple tissue types and sizes Active and passive clearing modes
        Active and passive clearing
        modes
      • Tissue Clearing System II Compatible with multiple tissue types and sizes Holders of various sizes available
        Holders of various sizes
        available
    • User-friendly setup
      • X-CLARITY Tissue Clearing System II User-friendly setup Simple touchscreen interface
        Simple touchscreen
        interface
      • X-CLARITY Tissue Clearing System II User-friendly setup Ready-to- use clearing solution
        Ready-to-use clearing
        solution
    • Electrophoretic Tissue Clearing Solution C13001
      Electrophoretic Tissue Clearing Solution is a premixed SDS-based buffer optimized for use with the X-CLARITY™ Tissue Clearing System II.
    • Electrophoretic Tissue Clearing Solution
    Whole Thy1-YFP mouse brain
    • Electrophoretic Tissue Clearing Solution tissue clearing before
    • Electrophoretic Tissue Clearing Solution tissue clearing after
    • Electrophoretic Tissue Clearing Solution tissue clearing imaging
    The Thy1-YFP signal is preserved and vibrant after tissue clearing with the X-CLARITY™ systems and reagents.
    Human cerebral cortex (1 mm)
    • X-CLARITY Mounting Solution Human cerebral cortex (1 mm) Before ETC PFA-fixed Seoul National University College of Medicine Hyung Jin Choi
      Before ETC
      PFA-fixed (1 week)
    • X-CLARITY Mounting Solution Human cerebral cortex (1 mm) After ETCSeoul National University College of Medicine Hyung Jin Choi
      After ETC
    • LogosBiosystems X-CLARITY Mounting Solution Human cerebral cortex (1 mm) After RI matching Seoul National University College of Medicine Hyung Jin Choi
      After RI matching
    Courtesy of Hyung Jin Choi, PhD
    Seoul National University College of Medicine
    ELECTROPHORETIC TISSUE CLEARING SOLUTION
      Cat # Product Qty MSDS Product Insert
      C13001 Electrophoretic Tissue Clearing Solution

      12 x 1 L

      FileAction
      [C13001] Electrophoretic Tissue Clearing Solution.pdfDownload 
      C13001 Electrophoretic Tissue Clearing Solution VX1802-01.pdfDownload 
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      NEXT

      LABELING AND IMAGING

      • Accelerate antibody labeling with the ALL-NEW DeepLabel™ Antibody Labeling Kit.
        LERN MORE
      • Antibody labeling DeepLabel Antibody Staining Kit
      BigStitcher: Reconstructing high-resolution image datasets of cleared and expanded samples D Hörl, FR Rusak, F Preusser, P Tillberg, N Rande, RK Chhetri, A Cardona, PJ Keller, H Harz, H Leonhardt, M Treier, S Preibisch bioRxiv 343954 (2018) SETBP1 induces transcription of a network of development genes by acting as an epigenetic hub Piazza R, Magistroni V, Redaelli S, Mauri M, Massimino L, Sessa A, Peronaci M, Lalowski M, Soliymani R, Mezzatesta C, Pirola A, Banfi F, Rubio A, Rea D, Stagno F, Usala E, Martino B, Campiotti L, Merli M, Passamonti F, Onida F, Morotti A, Pavesi F, Bregni M, Broccoli V, Baumann M, Gambacorti-Passerini C Nature Communications 9, 2192 (2018) PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types Gaire J, Lee HC, Ward R, Currlin S, Woolley AJ, Coleman JE, Williams JC, Otto KJ Scientific Reports 8, 7182 (2018) Subepithelial telocytes are an important source of Wnts that supports intestinal crypts Shoshkes-Carmel M, Wang YJ, Wangensteen KJ, Tóth B, Kondo A, Massassa EE, Itzkovitz S, Kaestner KH Nature 557, 242–246 (2018) Heart clearing opens new insights in the quantification of injured area after ischemia reperfusion Paccalet A, Badawi S, Gouriou Y, Bidaux G, Crola Da Silva C Archives of Cardiovascular Diseases Supplements 10(2), 254 (2018) Cortico–reticulo–spinal circuit reorganization enables functional recovery after severe spinal cord contusion Asboth L, Friedli L, Beauparlant J, Martinez-Gonzalez C, Anil S, Rey E, Baud L, Pidpruzhnykova G, Anderson MA, Shkorbatova P,, Batti L, Pagès S, Kreider J, Schneider BL, Barraud Q, Courtine G. Nature Neuroscience 21, 576-588 (2018) 3D imaging in the postmortem human brain with CLARITY and CUBIC Ando K, Laborde Q, Brion JP, Duyckaerts C Handbook of Clinical Neurology 150, 303-317 Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, Duan H, Ma P, Zhang L, Zamvil SS, Hidalgo J, Zhang Z, O'Rourke DM, Dahmane N, Brem S, Mou Y, Gong Y, Fan Y Nature Communications 9, 559 (2018) Pitch-tunable pillar arrays for high-throughput culture and immunohistological analysis of tumor spheroids Lee DW, Kang J, Hwang HJ, Oh MS, Shin BC, Lee MY, Kuh HJ RSC Advances 8, 4494-4502 (2018) Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome Lamm KYB, Johnson ML, Phillips JB, Muntifering MB, James JM, Jones HN, Redline RW, Rokas A, Muglia, LJ eLife 7: e31150 (2018) Rapid spheroid clearing on a microfluidic chip Santisteban TS, Rabajania O, Kalinina I, Robinson S, Meier M Lab on a Chip 18, 153-161 (2018) Optical clearing for multi‐scale biological tissues Yu T, Qi Y, Gong H, Luo Q, Zhu D Journal of Biophotonics 11(2), e201700187 (2018) Organism-Level Analysis of Vaccination Reveals Networks of Protection across Tissues Kadoki M, Patil A, Thaiss CC, Brooks DJ, Pandey S, Deep D, Alvarez D, von Andrian UH, Wagers AJ, Nakai K, Mikkelsen TS, Soumillon M, Chevrier N Cell 171, 398-413 (2017) Effects of neural stem cell media on hypoxic injury in rat hippocampal slice cultures Lee NM, Chae SA, Lee HJ Brain Research 1677, 20-25 (2017) Optogenetic targeting of cardiac myocytes and non-myocytes: Tools, challenges and utility Johnston CM, Rog-Zielinska, EA, Wülfers EM, Houwaart T, Siedlecka U, Naumann A, Nitschke R, Knöpfel T, Kohl P, Shrneider-Warme F Progress in Biophysics & Molecular Biology 130, 140-149 (2017) Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis Dubey LK, Karempudi P, Luther SA, Ludewig B, Harris NL Nature Communications 8, 367 (2017) Advances and perspectives in tissue clearing using CLARITY Jensen KHR, Berg RW Journal of Chemical Neuroanatomy 86, 19-34 (2017) Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens Glaser AK, Reder NP, Chen Y, McCarty EF, Yin C, Wei L, Wang Y, True LD, Liu JTC Nature Biomedical Engineering 1, 0084 (2017) Graphene Oxide-Quenching-Based Fluorescence In Situ Hybridization (G-FISH) To Detect RNA In Tissue: Simple And Fast Tissue RNA Diagnostics Hwang DW, Choi YR, Kim D, Park HY, Kim KW, Kim MY, Park CK, Lee DS bioRxiv 143628 (2017) ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging Lee E, Choi J, Jo Y, Kim JY, Jang YJ, Lee HM, Kim SY, Lee HJ, Cho K, Jung N, Hur EM, Jeong SJ, Moon C, Choe Y, Rhyu IJ, Kim H, Sun W Scientific Reports 6, 618631 (2016)
      The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY Poster DeepLabel™ Antibody Staining Kit Flyer X-CLARITY™ Brochure X-CLARITY™ Flyer C10001 X-CLARITY™ Tissue Clearing System Quick Start Guide C30001 X-CLARITY™ Tissue Clearing System II Installation Guide Preserving endogenous fluorescent protein signals for volumetric imaging using the X-CLARITY™ systems and reagents for tissue clearing ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging The X-CLARITY™ Mounting Solution: an improved RI matching solution for tissues cleared by the X-CLARITY™ Tissue Clearing System An automated and high-throughput polymerization solution for downstream tissue clearing: the X-CLARITY™ Polymerization System Logos Biosystems Products Brochure X-CLARITY Tissue Clearing System II S/W ver 1.1.0 X-CLARITY Polymerization S/W ver 1.1.0

      WHAT DO OUR CUSTOMERS HAVE TO SAY?

      Testimonials
      • Chiara Magliaro, PhD

        Research Center E. Piaggio - University of Pisa

        The X-CLARITY system is a very easy-to-use and versatile instruments. I started using it for murine brains, and it was quite easy to customize the protocols also for different biological samples (e.g., 3D advanced neural constructs). It allows me to avoid both waste of time and money.

      • Dongyue Lin, PhD

        Sun Yat-Sen University

        Great results. The instrument is very convenient, and the tissue clearing effect is very good.

      • Francesca Longhena, PhD

        University of Brescia

        Essential for good clarification! This instrument is very useful in order to make the clarification protocol easier and faster!"

      • Cesare Covino

        San Raffaele Scientific Institute

        X-CLARITY is the definitive solution for an effective, robust and highly repeatable clearing. X-CLARITY helps clearing expert users to have more reproducible and standardized data, opening the possibility to have robust quantitative data even from cleared tissue samples, as well as dummies that look out this technique for the first time. In our facility very often people who had never even been done just simple immunofluorescences they were able to get perfect cleared samples for imaging (confocal or two-photon).

      • Claire Crola Da Silva, PhD

        INSERM U1060

        It is essential for our research. Very easy to use. It gives robust and reproducible results and allows us to standardize the methodological chain.

      • Victoria Neckles

        Clemson University

        Great results, easy to use! This product was effective at clearing my tissue samples. It cleared whole brains, hearts, stomach, kidneys, livers, etc. The user manual was easy to read and easy to follow. There is little attention and maintenance required while the system in running.

      • Terika Smith, PhD

        University of South Carolina

        Great instrument and would highly recommend. This tissue clearing system is very easy to use and is very user friendly. I've used it to clear mouse nerves and spinal cords in approximately 4-6 hours. When the system first arrived, there was an issue with the ETC controller, but customer service handled it very efficiently and quickly. We've been getting great results with this system and I would highly recommend it to anyone interested in tissue clearing.

      • Alexandre Paccalet, PhD

        INSERM

        Confident system and confident company. I work with the X-CLARITY system every day and I can say that this is a very reliable system. The simpler utilization and the ergonomics of the machine is appreciable. The customer service cannot be more efficient, any problems are resolved in a day or two day maximum and communication is based on mutual confidence. I recommend Logos and the X-CLARITY system.

      • Gabriel Bidaux, PhD

        INSERM UMR 1060

        For you if you want to accelerate your research! The X-CLARITY system is easy to use, devoted to aqueous-based method for organ clearing. It helps clearing several organs at once and in a short period of time. Works well on a lot of different mouse organs or even pieces of primates and humans organs. Logos Biosystems after-sales are fast and always find a compromise as a solution to our rare issues.

      • Doug Richardson, PhD

        Harvard Center for Biological Imaging

        Logos Biosystems Harvard Center for Biological Imaging Doug Richardson X-CLARITY Tissue Clearing System

        We purchased the X-CLARITY Tissue Clearing System for our facility and have been very satisfied with its easy-to-use design and consistent results. The X-CLARITY Tissue Clearing System has now allowed many more researchers to enter the field of tissue clearing. In my opinion, the X-CLARITY Tissue Clearing System along with the recently introduced X-CLARITY Polymerization System will help expand the availability of this technique to all scientists who hope to add it to their laboratory’s repertoire.

      • David Mulder

        Universität Hamburg

        Great product, produces rich data. So far, the X-CLARITY has helped us to get amazing data on astrocyte morphology in the olfactory bulb. Thanks to the clearing, it makes slicing the tissue for microscopy redundant. Therefore we are able to study the more complex networks in their physiological form. Great product!

      • Uri Manor, PhD

        Salk Institute for Biological Studies

        The X-CLARITY system is a delight to use and significantly lowers the barrier to tissue clearing imaging applications. With better clearing, and faster turnaround time, we were able to generate many more samples and images than would otherwise have been possible. Highly recommended!

      • Yoko Yazaki-Sugiyama, PhD

        Okinawa Institute of Science and Technology

        For quite a while we were using passive immersion method to obtain transparent zebra finch brains, but this was time-consuming and always a pain in the neck. With the X-CLARITY Tissue Clearing System, even a whole brain can be cleared within several hours with excellent consistency as well as better signal-to-noise ratio when acquiring fluorescent images from deep tissue. It has made our lives a lot easier. TIME IS MONEY!

      • Robert Hunt, PhD

        University of California, Irvine

        We were not sure what to expect when we tried the X-CLARITY Tissue Clearing System. I've tried other tissue clearing methods with varying levels of success. When Jan, a first-year graduate student, showed me a fully cleared whole mouse brain on his first try, I was really impressed! I think this system will really speed up his work.

      • Annick Prigent & Benoît Delatour, PhD

        ICM Histomics

        Logos Biosystems ICM Histomics Annick Prigent & Benoît Delatour X-CLARITY Tissue Clearing System

        The X-CLARITY allows us to perform rapid, efficient, and standardized clearing of mouse and human brain tissues. Access to the X-CLARITY technology will undoubtedly help the ICM research teams to better understand the 3D organization of protein assemblies and organelles in tissues.

      • Woong Sun, PhD

        Korea University Medical School

        Logos Biosystems Korea University Medical School Woong Sun X-CLARITY Tissue Clearing System

        I tried to design my own DIY ETC chamber for a long time without any success. I tried the X-CLARITY Tissue Clearing System and got a transparent mouse brain on my first try. It works really well and is easy to use. I’ve successfully imaged tissues that contain GFP or that I’ve labeled with fluorescence-tagged antibodies. It makes tissue clearing a stress free process.

      • Youngshik Choe, PhD

        Korea Brain Research Institute

        Logos Biosystems Korea Brain Research Institute Youngshik Choe X-CLARITY Tissue Clearing System

        I find the X-CLARITY simplifies the CLARITY procedure and gives consistent transparency of tissues by plug and play steps.
        The best of it is the speed that can't be achieved by other methods.

      + SHOW OUR CUSTOMERS

      WHERE THE X-CLARITY™ HAS BEEN CITED

      Citations
      • Neuroprotective effect of nerve growth factor loaded in porous silicon nanostructures in an Alzheimer's disease model and potential delivery to the brain.
        2019. Zilony-Hanin N, Rosenberg M, Richman M, Yehuda R, Schori H, Motiei M,, Rahimipour S, Groisman A, Segal E, Shefi O. Small e1904203.

      • Multiplex immunolabeling and imaging of functionally essential kidney structures in X-CLARITY-cleared tissue.
        2019. Mochama P, Tyshynsky R, Sanders MA. Microscopy and Microanalysis 25(Supplement S2): 1260-1261.

      • BigStitcher: Reconstructing high-resolution image datasets of cleared and expanded samples.
        2019. Hörl D, Rusak FR, Preusser F, Tillberg P, Rande N, Chhetri RK, Cardona A, Keller PJ, Harz H, Leonhardt H, Treier M, Preibisch S. Nature Methods doi.org/10.1038/s41592-019-0501-0.

      • Clarity analysis of the Cl/pH sensor expression in the brain of transgenic mice.
        2019. Diuba AV, Samigullin DV, Kaszas A, Zonfrillo F, Malkov A, Peukhova E, Casini A, Arosio D, Esclapez M, Gross CT, Bregestovski P. Neuroscience.

      • Enhanced resolution 3D digital cytology and pathology with dual-view inverted selective plane illumination microscopy.
        2019. Hu B, Li G, Brown JQ. Biomedical Optics Express 10(8): 3833-3846.

      • High-performance acellular tissue scaffold combined with hydrogel polymers for regenerative medicine.
        2019. Lee E, Kim HJ, Shaker MR, Ryu JR, Ham MS, Seo SH, Kim DH, Lee K, Jung N, Choe Y, Son GH, Ryu IJ, Kim H, Sun W. ACS Biomaterials Science & Engineering 5(7): 3462-3474.

      • Presynaptic boutons that contain mitochondria are more stable.
        2019. Lees RM, Johnson JD, Ashby MC. bioRxiv 580530.

      • A HaloTag-TEV genetic cassette for mechanical phenotyping of native proteins.
        2019. Rivas-Pardo JA, Li Y, Mártonfalvi Z, Tapia-Rojo R, Unger A, Fernández-Trasancos Á, Herrero-Galán E, Velázquez-Carreras D, Linke WA, Fernández JM, Alegre-Cebollada J. bioRxiv 577445.

      • Development of sample-adaptable holders for lightsheet microscopy.
        2019. Laroche T, Burri O, Dubey LK, Seitz A. Frontiers in Neuroanatomy 13:26.

      • A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesicles.
        2019. Neckles VN, Morton MC, Holmberg JC, Sokolov AM, Nottoli T, Liu D, Feliciano DM. Scientific Reports 9(1): 3094.

      • A novel optical tissue clearing protocol for mouse skeletal muscle to visualize endplates in their tissue context.
        2019. Williams MPI, Rigon M, Straka T, Hörner SJ, Thiel M, Gretz N, Hafner M, Reischl M, Rudolf R. Frontiers in Cellular Neuroscience 13: 49.

      • Bioinspired neuron-like electronics.
        2019. Yang X, Zhou T, Zwang TJ, Hong G, Zhao Y, Viveros RD, Fu TM, Gao T, Lieber CM. Nature Materials.

      • A novel population of long-range inhibitory neurons.
        2019. Wick ZC, Tetzlaff MR, Krook-Magnuson E. bioRxiv 554360.

      • Expansion light sheet microscopy resolves subcellular structures in large portions of the songbird brain.
        2019. Düring DN, Rocha MD, Dittrich F, Gahr M, Hahnloser RHR. Frontiers in Neuroanatomy 13:2.

      • Graphene oxide-quenching-based fluorescence in situ hybridization (G-FISH) to detect RNA in tissue: Simple and fast tissue RNA diagnostics.
        2018. Hwang DW, Choi YR, Kim D, Park HY, Kim KW, Kim MY, Park CK, Lee DS. Nanomedicine 16, 162-172.

      • Aberrant accrual of BIN1 near Alzheimer's disease amyloid deposits in transgenic models.
        2018. De Rossi P, Andrew RJ, Musial TF, Buggia-Prevot V, Xu G, Ponnusamy M, Ly H, Krause SV, Rice RC, de l'Estoile V, Valin T, Salem S, Despa F, Borchelt DR, Bindokas VP, Nicholson DA, Thinakaran G. Brain Pathology.

      • Specimen preparation.
        2018. Jerome WG, Fuseler J, Padgett CA, Price RL. ). Basic Confocal Microscopy, 73–97.

      • Combined structural and functional imaging of the kidney reveals major axial differences in proximal tubule endocytosis.
        2018. Schuh CD, Polesel M, Platonova E, Haenni D, Gassama A, Tokonami N, Ghazi S, Bugarski M, Devuyst O, Ziegler U, Hall AM. Journal of the American Society of Nephrology, 29(11): 2696-2712.

      • Optical tissue clearing in combination with perfusion and immunofluorescence for placental vascular imaging.
        2018. Carrillo M, Chuecos M, Gandhi K, Bednov A, Moore DL, Maher J, Ventolini G, Ji G, Schlabritz-Loutsevitch N. Medicine (Baltimore) 97(39), e12392.

      • Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures.
        2018. Kim JH, Jang MJ, Choi J, Lee E, Song KD, Cho J, Kim KT, Cha HJ, Sun W. Scientific Reports, 8(1):12815.

      • The neuroregenerative capacity of olfactory stem cells is not limitless: Implications for aging.
        2018. Child KM, Herrick DB, Schwob JE, Holbrook EH, Jang W. Journal of Neuroscience 38(31), 6806-6824.

      • SETBP1 induces transcription of a network of development genes by acting as an epigenetic hub.
        2018. Piazza R, Magistroni V, Redaelli S, Mauri M, Massimino L, Sessa A, Peronaci M, Lalowski M, Soliymani R, Mezzatesta C, Pirola A, Banfi F, Rubio A, Rea D, Stagno F, Usala E, Martino B, Campiotti L, Merli M, Passamonti F, Onida F, Morotti A, Pavesi F, Bregni M, Broccoli V, Baumann M, Gambacorti-Passerini C. Nature Communications 9, 2192.

      • PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types.
        2018. Gaire J, Lee HC, Ward R, Currlin S, Woolley AJ, Coleman JE, Williams JC, Otto KJ. Scientific Reports 8, 7182.

      • Subepithelial telocytes are an important source of Wnts that supports intestinal crypts.
        2018. Shoshkes-Carmel M, Wang YJ, Wangensteen KJ, Tóth B, Kondo A, Massassa EE, Itzkovitz S, Kaestner KH. Nature 557, 242–246.

      • Latrophilin 2 specifies cardiac lineage commitment and heart development.
        2018. Lee CS, Cho HJ, Lee JW, Lee J, Kwon YW, Park H, Kim J, Kim HS. SSRN Electronic Journal.

      • Heart clearing opens new insights in the quantification of injured area after ischemia reperfusion.
        2018. Paccalet A, Badawi S, Gouriou Y, Bidaux G, Crola Da Silva C. Archives of Cardiovascular Diseases Supplements 10(2), 254.

      • Cortico–reticulo–spinal circuit reorganization enables functional recovery after severe spinal cord contusion.
        2018. Asboth L, Friedli L, Beauparlant J, Martinez-Gonzalez C, Anil S, Rey E, Baud L, Pidpruzhnykova G, Anderson MA, Shkorbatova P,, Batti L, Pagès S, Kreider J, Schneider BL, Barraud Q, Courtine G. Nature Neuroscience 21, 576-588.

      • 3D imaging in the postmortem human brain with CLARITY and CUBIC.
        2018. Ando K, Laborde Q, Brion JP, Duyckaerts C. Handbook of Clinical Neurology 150, 303-317.

      • Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.
        2018. Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, Duan H, Ma P, Zhang L, Zamvil SS, Hidalgo J, Zhang Z, O'Rourke DM, Dahmane N, Brem S, Mou Y, Gong Y, Fan Y. Nature Communications 9, 559.

      • Pitch-tunable pillar arrays for high-throughput culture and immunohistological analysis of tumor spheroids.
        2018. Lee DW, Kang J, Hwang HJ, Oh MS, Shin BC, Lee MY, Kuh HJ. RSC Advances 8, 4494-4502.

      • Inverted formin 2 regulates intracellular trafficking, placentation, and pregnancy outcome.
        2018. Lamm KYB, Johnson ML, Phillips JB, Muntifering MB, James JM, Jones HN, Redline RW, Rokas A, Muglia LJ. eLife 7: e31150.

      • Rapid spheroid clearing on a microfluidic chip.
        2018. Santisteban TS, Rabajania O, Kalinina I, Robinson S, Meier M. Lab on a Chip 18, 153-161.

      • Optical clearing for multi‐scale biological tissues.
        2018. Yu T, Qi Y, Gong H, Luo Q, Zhu D. Journal of Biophotonics 11(2), e201700187.

      • Organism-level analysis of vaccination reveals networks of protection across tissues.
        2017. Kadoki M, Patil A, Thaiss CC, Brooks DJ, Pandey S, Deep D, Alvarez D, von Andrian UH, Wagers AJ, Nakai K, Mikkelsen TS, Soumillon M, Chevrier N. Cell 171, 398-413.

      • Effects of neural stem cell media on hypoxic injury in rat hippocampal slice cultures.
        2017. Lee NM, Chae SA, Lee HJ. Brain Research 1677, 20-25.

      • Optogenetic targeting of cardiac myocytes and non-myocytes: Tools, challenges and utility.
        2017. Johnston CM, Rog-Zielinska, EA, Wülfers EM, Houwaart T, Siedlecka U, Naumann A, Nitschke R, Knöpfel T, Kohl P, Shrneider-Warme F. Progress in Biophysics & Molecular Biology 130, 140-149.

      • Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis.
        2017. Dubey LK, Karempudi P, Luther SA, Ludewig B, Harris NL. Nature Communications 8, 367.

      • Advances and perspectives in tissue clearing using CLARITY.
        2017. Jensen KHR, Berg RW, Journal of Chemical Neuroanatomy 86, 19-34.

      • Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens.
        2017. Glaser AK, Reder NP, Chen Y, McCarty EF, Yin C, Wei L, Wang Y, True LD, Liu JTC. Nature Biomedical Engineering 1, 0084.

      • ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging.
        2016. Lee E, Choi J, Jo Y, Kim JY, Jang YJ, Lee HM, Kim SY, Lee HJ, Cho K, Jung N, Hur EM, Jeong SJ, Moon C, Choe Y, Rhyu IJ, Kim H, Sun W. Scientific Reports 6, 618631.

      SHOW MORE  +

      Resources

      Downloads
      • Icon
        Logos Biosystems Products

        Brochure

        2019-07-16 | 2.14 MB
      • Icon
        The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY

        Poster

        2018-11-26 | 1.25 MB
      • Icon
        DeepLabel™ Antibody Staining Kit Flyer VDL1810-01

        Brochure

        2018-11-02 | 458.10 KB
      • Icon
        X-CLARITY™ Systems and Reagents for Tissue Clearing Brochure VX1901-01

        Brochure

        2018-05-18 | 962KB
      • Icon
        X-CLARITY™ Systems and Reagents for Tissue Clearing Flyer VX1810-01

        Brochure

        2018-05-08 | 553 KB
      • Icon
        C10001 X-CLARITY™ Tissue Clearing System Quick Start Guide

        Quick Start Guide

        2018-05-08 | 668.54 KB
      • Icon
        C30001 X-CLARITY™ Tissue Clearing System II Installation Guide

        Installation Guide

        2018-05-08 | 841.04 KB
      • Icon
        Preserving endogenous fluorescent protein signals for volumetric imaging using the X-CLARITY™ systems and reagents for tissue clearing

        Product literature

        2018-04-17 | 1.6 MB
      • Icon
        ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

        Product literature

        2018-04-17 | 2.6 MB
      • Icon
        The X-CLARITY™ Mounting Solution: an improved RI matching solution for tissues cleared by the X-CLARITY™ Tissue Clearing System

        Product literature

        2018-04-17 | 1.5 MB
      • Icon
        X-CLARITY Tissue Clearing System II S/W ver 1.1.0

        Software

        2018-06-27 | 504KB
      • Icon
        X-CLARITY Polymerization S/W ver 1.1.0

        Software

        2018-06-26 | 452KB
        Videos

        Basic Principles of Clearing and Imaging Biological Tissues

        Single-neuron segmentation of 3D confocal images of clarified tissues

        DataBrain: Using deep imaging techniques to develop a high-fidelity map of the brain

        Multiscale analysis of ischemia-reperfusion syndrome.

        Studying molecular mechanisms of ischemia-reperfusion.

        Polymerization simplified

        The new X-CLARITY™ sample holders

        Innovations in Spinal Treatment using Electrophoretic Tissue Clearing

        Spinal Treatment Techniques in the Lab

        How the CLARITY Method Supports Spinal Cord Research

        A journey through the brain

        Whole mouse brain cleared

        Biocompare explains CLARITY

        Mouse brain slice cleared

        ORDERING INFORMATION

        Cat # Product Qty
        C20001 X-CLARITY™ Polymerization System 1 unit
        C20002 X-CLARITY™ Heat Block for 6 x 50 mL tubes 1 unit
        C20003 X-CLARITY™ Heat Block for flat-bottom plates 1 unit
        C1310X X-CLARITY™ Hydrogel Solution Kit 1 kit
        C30001 X-CLARITY™ Tissue Clearing System II 1 unit
        C12010 Holder for 36 Mouse Brain Slices (1.5 Φ) 1 set
        C12020 Holder for 36 Mouse Brain Slices (0.6 Φ) 1 set
        C12011 Holder for 6 Slices (1.5 Φ) 1 set
        C12021 Holder for 6 Slices (0.6 Φ) 1 set
        C12012 Holder for 1 Sample (1.5 Φ) 1 set
        C12022 Holder for 1 Sample (0.6 Φ) 1 set
        C12013 Holder for 6 Mouse Brains (1.5 Φ) 1 set
        C12023 Holder for 6 Mouse Brains (0.6 Φ) 1 set
        C12014 Holder for 48 Samples (1.5 Φ) 1 set
        C12024 Holder for 48 Samples (0.6 Φ) 1 set
        C12015 Holder for 192 Samples (1.5 Φ) 1 set
        C12025 Holder for 192 Samples (0.6 Φ) 1 set
        C12001 Tissue Container (20 units) 1 box
        C12002 Container Holder for 1 Tissue Container 1 unit
        C12004 Mouse Brain Slice Holder 1 unit
        C12007 Whole Rat Brain Holder 1 unit
        C13001 Electrophoretic Tissue Clearing Solution 12 x 1 L
        C13101 X-CLARITY™ Mounting Solution 1 x 25 mL
        C13102 X-CLARITY™ Mounting Solution Value Pack 10 x 25 mL
        C13107 X-CLARITY™ Mounting Solution Bulk Pack 20 x 25 mL
        C33001 DeepLabel™ Antibody Staining Kit 1 kit