Early Cytokine Induction Upon Pseudomonas aeruginosa Infection in Murine Precision Cut Lung Slices Depends on Sensing of Bacterial Viability.
2020. Kolbe U, Yi B, Poth T, Saunders A, Boutin S, Dalpke AH. Frontiers in Immunology 11:598636.
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Microbial Cell Counter
QUANTOM Tx™ Microbial Cell Counter
No more waiting days to count CFUs. In less than 30 seconds, the QUANTOM Tx™ scans up to 10 fields of view to produce accurate single bacterial cell counts. The advanced software can account for the varying shapes, sizes, and arrangements of different bacteria.download software
Although smaller and simpler than their eukaryotic counterparts, bacteria are an incredibly diverse group of organisms that come in multiple shapes, sizes, and arrangements. Colony counting is a highly variable and unreliable, as it is only an estimate of the viable cells present. Even expensive flow cytometers and laser scanning cytometers register each particle, single or clustered, as a single event. The QUANTOM Tx™ Microbial Cell Counter is an image-based, automated cell counter that can detect individual bacterial cells in mere minutes. Its novel bacteria-optimized cell detection software can count individual bacterial cells in even the tightest clusters.
WHAT THE QUANTOM Tx™ DOES BEST
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RESULTS IN MINUTES, NOT DAYSMinutes to results
No culturing required -
ACCURATE RESULTSObjective and no user-to-user variability
No estimating based on colony forming units or turbidity -
SINGLE BACTERIUM DETECTIONRegardless of cell shape, size, or arrangement
Advanced bacterial cell counting software -
TOTAL & VIABLE CELL COUNTSFluorescence stains for accurate cell detection
Multiple images captured and analyzed
THE SOFTWARE THAT CHANGES EVERYTHING.
INNOVATIVE COUNTING PROCESS ENSURES ACCURATE COUNTS
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Staining
QUANTOM™ Total Cell Staining Dye easily permeates both gram positive and gram negative bacteria to stain both live and dead cells. QUANTOM™ Viable Cell Staining Dye efficiently labels viable bacteria.
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Immobilizing
Stained cells are mixed with our bacteria-optimized loading buffer, loaded into QUANTOM™ M50 Cell Counting Slides, and spun in the QUANTOM™ Centrifuge to immobilize and evenly distribute the cells throughout the counting chamber to ensure accurate cell counts.
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Counting
The QUANTOM Tx™ captures up to 10 high resolution images and counts the cells in each. The highly sophisticated software can distinguish individual cells in various arrangements such as tight clusters or in sequence to produce accurate and reliable total bacterial cell counts.
Compatible Reagents
Request CoA >
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WHAT DO OUR CUSTOMERS HAVE TO SAY?
Testimonials
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Nicolas Kong
Servatus Ltd
Overall, we are very satisfied with the platform. It is a mainstay of our workflow.
We use the Logos QUANTOM Tx (QTx) to enumerate various species of bacteria grown in liquid media. Prior to purchasing the QTx, enumeration was done via the spread-plate method. The rapid quantitation afforded by the QTx allows us to have a result in as little as 30 minutes, compared to 3 days for the spread-plate method. We have also found the QTx useful for determining the proportion of live/dead cells within a sample. The simplicity and ease of use of the QTx allows for rapid training of new operators and excellent repeatability. In addition, Logos' after-sales service has been exceptional. -
Nermina M.
University of Graz
Makes it possible to discover membrane active components.
Easy to handle, although I just started using the instrument and I have the impression I can do more with it. We are using it to count simple bacterial cells which we need to do in order to start antibacterial susceptibility assays. The drugs which we test against diverse bacterial cells are membrane active. Therefore, total as well as live/dead assays provide additional information for us. If there was a future upgrade for this instrument, I would definitely think about including optical filters for different wavelengths covering a broader fluorescence range. Then we could play with difference membrane permeable/impermeable dyes. -
Laila Phillips
Sinai Hospital Division of Gastroenterology
The QUANTOM Tx is extremely user-friendly with a beautiful, intuitive interface.
The entire process from start to finish is very quick, so we were able to gather a lot of data in a short amount of time. We mastered the staining protocol very quickly, and even the more inexperienced members of our research group were able to use the machine properly with ease. We were blown away by the quality of the images we received, even in mixed cultures of bacteria gathered from various biological samples! -
Violetta Medik, MS
Evelo Biosciences
Great results. I am very happy with the instrument.
The instrument is very easy to use. The sample prep is quick and the the replicates are pretty tight. I used it for counting very small cells, as well as bacteria that grow in chains or clusters and the software does a great job analyzing the image. I compared the results to other methods and the results are spot on. Overall, I am very happy with the purchase.
WHERE THE quantom tx™ HAS BEEN CITED
Citations
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Low-Carbohydrate Tolerant LAB Strains Identified from Rumen Fluid: Investigation of Probiotic Activity and Legume Silage Fermentation.
2020. Kuppusamy P, Kim D, Soundharrajan I, Park HS, Jung JS, Yang SH, Choi KC. Microorganisms 8(7):E1044. -
Potential clinical application of an automated fluorescent microbial cell counter in the detection of urinary tract infection.
2020. Phillips LE, Verma S, Surapaneni BK, Dutta SK. Journal of Clinical Laboratory Analysis 4:e23334. -
Probiotic Characteristics and Antifungal Activity of Lactobacillus plantarum and Its Impact on Fermentation of Italian Ryegrass at Low Moisture.
2020. Muthusamy K, Soundharrajan I, Sriseharam S, Kim DH, Kuppusamy P, Lee KD, Choi KC. Applied Sciences 2020, DOI:10.3390/app10010417. -
Effect of structural condition of milk processing facilities and food safety systems on Escherichia coli and coliforms presence in cultured buttermilk.
2019. Paraffin AS, Zindove TJ, Chimonyo M. Journal of Food Quality 2019: 7365983. -
Role of acid responsive genes in the susceptibility of Escherichia coli to ciclopirox.
2018. Shin J, Cho H, Kim S, Kim KS. Biochemical and Biophysical Research Communications 500(2): 296-301. -
Anti-oxidative effects of silkworm storage protein 1 in HeLa cell.
2017. Lee JH, Baik JE, Rhee WJ. Process Biochemistry 55: 199-206. -
Potential usefulness of Streptococcus pneumoniae extracellular membrane vesicles as antibacterial vaccines.
2017. Choi CW, Park EC, Yun SH, Lee SY, Kim SI, Kim GH. Journal of Immunology Research 2017: 7931982.
Resources
Downloads
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Logos Biosystems ProductsBrochure
2020-04-26 | 2.14 MB -
QUANTOM Tx™ Quick Start Guide VQ1906-01
Quick Start Guide
2019-06-17 | 496.74 KB -
QUANTOM Tx™
FAQ
2019-06-17 | 812 KB -
QUANTOM Tx™ flyer VQ1906-01
Brochure
2019-06-17 | 646 KB -
QUANTOM Tx™ Microbial Cell Counter for rapid enumeration: comparison with existing methods
Product literature
2018-04-16 | 1.1 MB
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QUANTOM Tx™ software 1.9.0Software
2019-06-17 | 3 MB -
Using QUANTOM™ Utility to transfer data via wifi VQ1903-01
Notice
2019-03-26 | 245 KB -
QUANTOM™ Utility 1.2.0 (Win 10, 64-bit)
Software
2018-04-17 | 17.9 MB -
QUANTOM™ Utility 1.2.0 (Win 10, 32-bit)
Software
2018-04-17 | 15.7 MB
Cell DB
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Alcaligenes faecalis
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Protocol name: DEFAULT
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 3
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Bacillus amyloliquefaciens
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 0
Declustering level: 7
Detection sensitivity: 3
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Bacillus coagulans
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 0
Declustering level: 10
Detection sensitivity: 0
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Bacillus megaterium
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 0
Declustering level: 7
Detection sensitivity: 0
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Bacillus mycoides
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 0
Declustering level: 7
Detection sensitivity: 0
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Bacillus subtilis
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 0
Declustering level: 7
Detection sensitivity: 0
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Enterobacter aerogenes
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Protocol name: DEFAULT
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 3
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Enterobacter cloacae
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Protocol name: DEFAULT
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 3
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Escherichia coli
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 3
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Lactobacillus casei
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 0
Declustering level: 10
Detection sensitivity: 3
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Proteus mirabilis
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 0
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Pseudomonas fluorescens
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 0
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Pseudomonas putida
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 0
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Pseudomonas stutzeri
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Protocol name: DEFAULT
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 3
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Serratia marcescens
Stain: QUANTOM™ Total Cell Staining Dye
Counting parameters
Protocol name: DEFAULT
Min. fluorescent object size: 1 µm
Max. fluorescent object size: 50 µm
Roundness: 30
Declustering level: 7
Detection sensitivity: 3
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ORDERING INFORMATION
| Cat # | Product | Qty |
|---|---|---|
| Q10001 | QUANTOM Tx™ Microbial Cell Counter | 1 unit |
| Q10002 | QUANTOM™ Centrifuge | 1 unit |
| Q12001 | QUANTOM™ M50 Cell Counting Slides, 50 Slides | 1 box |
| Q12002 | QUANTOM™ M50 Cell Counting Slides, 500 Slides | 10 boxes |
| Q13501 | QUANTOM™ Total Cell Staining Kit - QUANTOM™ Total Cell Staining Dye (1 x 0.25 mL) - QUANTOM™ Total Cell Staining Enhancer (1 x 0.5 mL) - QUANTOM™ Cell Loading Buffer I (2 x 1 mL) |
1 kit |
| Q13502 | QUANTOM™ Viable Cell Staining Kit - QUANTOM™ Viable Cell Staining Dye (2 x 50 µg) - Dimethyl Sulfoxide (2 x 1 mL) - QUANTOM™ Viable Cell Dilution Buffer (1 x 20 mL) - QUANTOM™ Cell Loading Buffer I (2 x 1 mL) |
1 kit |
| Q13102 | QUANTOM™ Calibration Beads | 1 x 0.5 mL |