• Fluorescence Cell Counter

    LUNA-FL™ Dual Fluorescence Cell Counter

    Equipped with dual fluorescence and brightfield optics, the LUNA-FL™ is a powerhouse. The LUNA-FL™ can assess cell counts, viability, and GFP transfection efficiency without being limited by cell type or size.
    download software
With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

WHAT THE LUNA-FL™ DOES BEST

  • TWO COUNTERS IN ONE

    The LUNA-FL™ can count in both brightfield and fluorescence mode, making
    it our most versatile counter to date.

  • COMPATIBLE WITH MOST CELLS

    Advanced imaging software and GFP and RFP fluorescence optics sensitively
    detect fluorescence stained cells.

  • GFP TRANSFECTION ANALYSIS

    High resolution images are captured and analyzed to detect GFP-positive and -negative cells.

  • MAXIMUM 30 SECONDS

    Fluorescence: 30 s = 3 images captured & analyzed;
    Brightfield: 10 s = 1 image captured & analyzed

THE SOFTWARE THAT CHANGES EVERYTHING.

FOUR COUNTING MODES CUSTOMIZE & SAVE PROTOCOLS USE THE DILUTION CALCULATOR GATE CELLS ON HISTOGRAMS

 

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Compatible Reagents
    Cat # Product Qty MSDS Product Insert
    T13001 Trypan Blue Stain, 0.4%

    2 x 1 mL

    FileAction
    [T13001] Trypan Blue Stain_0.4%.pdfDownload 
    T13001 Trypan Blue Stain, 0.4% VL1801-01.pdfDownload 
    L13002 Erythrosin B Stain

    2 x 1 mL

    FileAction
    [L13002] Erythrosin B Stain.pdfDownload 
    L13002 Erythrosin B Stain VL1801-01.pdfDownload 
    F23001 Acridine Orange/Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23001] Acridine Orange Propidium Iodide (AOPI) Stain.pdfDownload 
    F23001 Acridine Orange,Propidium Iodide Stain VL1801-01.pdfDownload 
    F23002 Acridine Orange Stain

    2 x 0.5 mL

    FileAction
    [F23002] Acridine Orange (AO) Stain.pdfDownload 
    F23002 Acridine Orange Stain VL1801-01.pdfDownload 
    F23003 Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23003] Propidium Iodide (PI) Stain.pdfDownload 
    F23003 Propidium Iodide Stain VL1801-01.pdfDownload 
    F23202 Yeast Viability Kit 1

    1 kit

    FileAction
    [F23202] Yeast Viability Kit 1.zipDownload 
    F23202 Yeast Viability Kit 1 VL1801-01.pdfDownload 
    Request CoA   >

    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Danh Tran

      Medical University of South Carolina

      Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.

    • Woong Sun, PhD

      Korea University Medical School

      I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.

    • May Malicdan, MD PhD

      NIH, NHGRI

      It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.

    • Joanna Ireland, PhD

      NIH, NIAID

      It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.

    + SHOW OUR CUSTOMERS

    WHERE THE LUNA-FL™ HAS BEEN CITED

    Citations
    • Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Application of a non-hazardous vital dye for cell counting with automated cell counters. Anal Biochem. 2016;492:8-12.

    • Ramirez K, Niraula A, Sheridan JF. GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations. Brain BehavImmun 2016;51:154-168.

    • Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M. Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles. Reprod Biol Endocrinol 2016;14:3

    • Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction. Int J Oncol 2016;48:2205-2212.

    • Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W. Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone. Stem Cells 2016;34:888-901.

    • Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C. Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts. J Med Virol 2016;88:1408-1416

    • Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells. J Nat Products 2016;79:317-323

    • Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury. J Surg Res 2016;204:361-370

    • Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. In vitro endothelialization test of biomaterials using immortalized endothelial cells. PLoS One 2016;11:e0158289

    • Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis. Sci Rep 2016;6:27301

    • Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Minicirclemicroporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site. Biomaterials 2016;101:310-320

    • Nagai K, Yanagawa Y, Katagiri S, Nagano M. The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicels. Biomed Res 2016;37:63-71

    • Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M. Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform. Nuc Acids Res 2016;44:e88

    • Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity. Nat Med 2016;22:547-556

    • Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H. Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells. Plasma Med 2016; 10.1615/PlasmaMed.2016016219

    • Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T. Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model. J BioactCompatPolym 2016; doi: 10.1177/0883911516635841

    • Ramirez K, Sheridan JF. Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors. Brain BehavImmun 2016; doi:10.1016/j.bbi.2016.05.008

    • Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses. J Vet Med Sci 2016; http://doi.org/10.1292/jvms.16-0124

    • Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model. J Pharmacol 2016; doi: 10.1124/jpet.116.233932

    • Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells. Exp Mol Med 2015;47:e143.

    • Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Brain BehavImmun 2015;46:212-220.

    • Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I. Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6. Int J Oncol 2015;47

    • Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T. Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones. Stem Cells Int 2015;2015:165867

    • Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG. Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor. Mol Cells 2015;38:548-561

    • Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury. J Surg Res 2015;

    • Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M. A mouse model for sublethalLeptospira infection. Infect Immun 2015;83:4693-4700.

    • Hong D, Lee G, Jung NC, Jeon M. Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images. Biol Proced Online 2013;15:13.

    SHOW MORE  +

    Resources

    Downloads
    • Icon
      LUNA-FL™: GFP Transfection Assay

      Quick Start Guide

      2018-04-16 | 110 KB
    • Icon
      LUNA-FL™

      Quick Start Guide

      2018-04-16 | 284 KB
    • Icon
      LUNA-FL™

      Brochure

      2018-04-16 | 1.1 MB
    • Icon
      Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images

      Product literature

      2018-04-16 | 1.7 MB
    • Icon
      Application of a non-hazardous vital dye for cell counting with automated cell counters

      Product literature

      2018-04-16 | 906 KB
    • Icon
      GFP expression analysis using the LUNA-FL™

      Product literature

      2018-04-16 | 403 MB
    • Icon
      Fast cell counting and viability measurement of yeast cells with the LUNA-FL™

      Product literature

      2018-04-16 | 862 KB
    • Icon
      Automated fluorescence cell counting

      Product literature

      2018-04-16 | 476 KB
    • Icon
      Logos Biosystems Products

      Brochure

      2018-04-15 | 193.4 MB
      • Icon
        LUNA-FL™ software 3.0.1

        Software

        2018-04-16 | 3.4 MB
      • Icon
        Autoexposure mode update

        Notice

        2018-04-16 | 2.2 MB
        Videos
        Cell DB
          • HL-60 (50% viability)

            Experimental condition

            HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

          • HL-60 (high density)

            Experimental condition

            A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

          • Human peripheral blood

            Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.

          • Jurkat

            Experimental condition

            Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

          • Mouse neuron

            Experimental condition

            Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

          • Mouse PBMC

            Experimental condition

            Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.

          • Mouse splenocyte

            Experimental condition

            Mouse splenocytes were collected directly from spleen tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.

          • Mouse thymocyte

            Experimental condition

            Mouse thymocytes were collected directly from the thymus tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.

          • Saccharomyces Cerevisiae (50% viability)

            Experimental condition

            Yeast (Saccharomyces Cerevisiae) cells were cultured in the YPD medium supplemented with several antibiotics overnight. The yeast cells were artificially killed by heating and mixed with healthy cells (50:50). Mixed cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter.

          • Size bead

            Experimental condition

            A high density 3 micron-sized beads were counted with the LUNA-FL to verifiy the performance of the instrument. Beads were counted at the bright field cell counting mode of LUNA-FL. Orange circles represent the automatically-classified objects by LUNA-FL.

          • Yeasts in a messy culture

            Experimental condition

            The number and the viability of yeast cells were measured in a bottle of a Korean rice wine. The wine was diluted at 1:100 with PBS, and the cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter

        show more  +

        ORDERING INFORMATION

        Cat # Product Qty
        L20001 LUNA-FL™ Automated Fluorescence Cell Counter 1 unit
        L12005 PhotonSlide™, 50 Slides 1 box
        L12006 PhotonSlide™, 500 Slides 10 boxes
        L12007 PhotonSlide™, 1000 Slides 20 boxes
        L12001 LUNA™ Cell Counting Slides, 50 Slides 1 box
        L12002 LUNA™ Cell Counting Slides, 500 Slides 10 boxes
        L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 boxes
        T13001 Trypan Blue Stain, 0.4% 2 X 1 mL
        L13002 Erythrosin B Stain 2 x 1 mL
        F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
        F23002 Acridine Orange Stain 2 x 0.5 mL
        F23003 Yeast Viability Kit 1
        - Propidium Iodide Stain for Yeast (1 x 0.5 mL)
        - Fluorescein Diacetate Stain (1 x 0.5 mL)
        - Cell Dilution Buffer (1 x 20 mL)
        - Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
        1 kit
        F23212 Cell Dilution Buffer 5 x 20 mL
        F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
        B13101 LUNA™ Standard Beads 2 x 1 mL
        P10001 LUNA™ Printer 1 unit
        P10002 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls