Precipitation of Trypan Blue (TB) solution is quite common and typically increases during storage when external temperatures decrease.
Figure 1. Comparison of 1 mL of TB (0.4% liquid solution) during storage at the 4-week mark at different external temperatures; TB solution made by two different manufacturers.
Warming precipitated TB solution to 37℃ for 10 minutes has been shown to effectively remove precipitation.
Figure 2. Comparison of 1 mL of TB (0.4% liquid solution) during storage at the 4-week mark at 4℃ and -20℃ and then with temperature increased to 37℃ for 10 minutes using Logos Biosystems TB solution.
|Trypan Blue Stain is a vital dye used to assess cell viability. Viable cells have intact cell membranes and are not stained, remaining colorless. Nonviable cells are stained blue.|
|Trypan Blue Stain is used with the LUNA™ family of cell counters for brightfield cell counting.|
|Trypan Blue Stain is a toxic compound, customers should take all relevant precautions when handling it (ie gloves).|
|When heating TB, it’s important to keep the cap on the tube, due to toxicity.|