Application Notes
How to Minimize Trypan Blue Precipitation
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Precipitation of Trypan Blue

Precipitation of Trypan Blue (TB) solution is quite common and typically increases during storage when external temperatures decrease.

Figure 1. Comparison of 1 mL of TB (0.4% liquid solution) during storage at the 4-week mark at different external temperatures; TB solution made by two different manufacturers.

Recovery of Precipitated Trypan Blue

Warming precipitated TB solution to 37℃ for 10 minutes has been shown to effectively remove precipitation.

Figure 2. Comparison of 1 mL of TB (0.4% liquid solution) during storage at the 4-week mark at 4℃ and -20℃ and then with temperature increased to 37℃ for 10 minutes using Logos Biosystems TB solution.

Product Details

Trypan Blue Stain is a vital dye used to assess cell viability. Viable cells have intact cell membranes and are not stained, remaining colorless. Nonviable cells are stained blue.
Trypan Blue Stain is used with the LUNA™ family of cell counters for brightfield cell counting.
Trypan Blue Stain is a toxic compound, customers should take all relevant precautions when handling it (ie gloves).
When heating TB, it’s important to keep the cap on the tube, due to toxicity.