FLUORESCENCE CELL COUNTER
LUNA-FL™ 自動蛍光セルカウンター

LUNA-FL™は、デュアル蛍光および明視野光学系を搭載した高性能セルカウンターです。細胞の種類や大きさに制限されることなく、細胞数、細胞生存率、そしてGFP形質転換効率を評価できます。

お見積もり・デモのご依頼はこちら
カウンターを比較する

4つのカウントモードを備えたLUNA-FL™は、まさに当社のフラッグシップモデルです。明視野およびデュアル蛍光光学系を搭載し、細菌を除くほとんどの細胞タイプを高感度で検出可能です。PBMC、脾臓細胞、好中球、幹細胞などの初代細胞を不要なデブリと区別し、正確な細胞数と生存率を測定します。明視野セルカウント、蛍光セルカウント、酵母セルカウント、GFP形質転換アッセイなど、多彩な用途に対応。コンパクトなデザインで、細胞培養フード内やベンチトップでも快適に使用でき、ワークフローにスムーズに組み込めます。

2台分の性能を1台に
LUNA-FL™は明視野および蛍光モードの両方で計測が可能。これまでで最も汎用性の高いセルカウンターです。
ほとんどの細胞に対応
高度なイメージングソフトウェアとGFP/RFP蛍光光学系により、蛍光染色された細胞を高感度に検出します。
GFP形質転換解析
高解像度の画像を取得し、GFP陽性および陰性細胞を自動で分析します。
最短30秒で完了
蛍光:30秒で3枚の画像を取得・分析 明視野:10秒で1枚の画像を取得・分析
すべてを変えるソフトウェア
最小限のコスト、最大限の利便性
コストセーブが懸案のラボに最適。LUNA™シリーズの自動セルカウンターは、LUNA™リユーザブルスライドに対応しています。手動カウントと同等の低コストを実現しながら、時間の節約、主観的な判断やユーザー間のばらつきを解消します。また、ディスポーザブルスライドを使用すれば、清掃不要で手間なく、最高レベルのカウント精度を維持できます。
お客様の声をご紹介
Testimonials
(5.0)
Moying Li

Great performance, would recommend for other researchers!
Easy to use, saves us time and offers good quality. The consultant Dr Eberhardt was also professional and patient to teach us before use.

University Heidelberg

(5.0)
Mahmood Ali

Great instrument, I like using it. Really helpful.
The device is easy to use, I mainly use it before cell seeding or to check for my culture's viability and survival. The results are reproducible and the device provides fast results. The accuracy seems to be great as well.

BGU

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LUNA-FL™ の引用実績
Citations

Brain Cancer Cell-derived Exosomes Protect Scopolamine-Induced Death of SH-SY5Y Neuron Cells.

2021. Lee M. International Journal of Health Sciences and Research 10.36838/v3i1.8.

The Type and Source of Reactive Oxygen Species Influences the Outcome of Oxidative Stress in Cultured Cells.

2021. Goffart S, Tikkanen P, Michell C, Wilson T, Pohjoismäki JLOLO. Cells 10(5):1075.

Immune Memory in Mild COVID-19 Patients and Unexposed Donors Reveals Persistent T Cell Responses After SARS-CoV-2 Infection.

2021. Ansari A, Arya R, Sachan S, Jha SN, Kalia A, Lall A, Sette A, Grifoni A, Weiskopf D, Coshic P, Sharma A, Gupta N. Frontiers in Immunology 12:636768.

The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System.

2021. Fujibayashi K, Mima J. Frontiers in Cell and Developmental Biology 9:628910.

Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids.

2021. Metzger W, Rösch B, Sossong D, Bubel M, Pohlemann T. Cell Biology International 10.1002/cbin.11618.

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Resources
Downloads
LUNA-FL™
brochure

2022-04-07 | 2.12 MB

Download
Logos Biosystems Products
brochure

2022-04-07 | 2.14 MB

Download
UNA-FL™: GFP Transfection Assay
Quick Start Guide

2018-04-16 | 110 KB

Download
LUNA-FL™
Quick Start Guide

2018-04-16 | 284 KB

Download
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images
Product literature

2018-04-16 | 1.7 MB

Download
Application of a non-hazardous vital dye for cell counting with automated cell counters
Product literature

2018-04-13 | 906 KB

Download

2018-04-16 | 476 KB

Download
Cell DB
HL-60 (high density)
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human peripheral blood
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
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Ordering Information
Cat # Product Qty
L20001 LUNA-FL™ Automated Fluorescence Cell Counter 1 unit
L12005 PhotonSlide™, 50 Slides 1 box
L12006 PhotonSlide™, 500 Slides 10 boxes
L12007 PhotonSlide™, 1000 Slides 20 boxes
L12001 LUNA™ Cell Counting Slides, 50 Slides 1 box
L12002 LUNA™ Cell Counting Slides, 500 Slides 10 boxes
L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 boxes
L12011 LUNA™ Reusable Slide 1 unit
L12012 LUNA™ Reusable Slides 2 units
L12014 LUNA™ Reusable Slide Coverslips 10 units
T13001 Trypan Blue Stain, 0.4% 2 X 1 mL
L13002 Erythrosin B Stain 2 x 1 mL
F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
F23002 Acridine Orange Stain 2 x 0.5 mL
F23003 Propidium Iodide Stain 2 x 0.5 mL
F23202 Yeast Viability Kit 1
– Propidium Iodide Stain for Yeast (1 x 0.5 mL)
– Fluorescein Diacetate Stain (1 x 0.5 mL)
– Cell Dilution Buffer (1 x 20 mL)
– Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
1 kit
F23212 Cell Dilution Buffer 5 x 20 mL
F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
B13101 LUNA™ Standard Beads 2 x 1 mL
F24003 LUNA-FL™ IQ/OQ Protocol 1 copy
P10001 LUNA™ Printer I 1 unit
P12001 LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls) 3 x 2 rolls
Specifications
Sample Volume 10 μl
Cell Counting Time 30 sec
Cell Concentration Range 5×104 – 1×107 cells/mL
Cell Size Range Detectable Range: 1 – 90 μm
Optimal Range: 5 – 60 μm
Excitation wavelength 470 ± 20 nm
Emission wavelength 530 ± 25 nm, 600 nm (LP)
Light Source LED
Image Resolution 5 MP
LCD Display 800 x 480 pixels
Dimensions (W×D×H) 22 × 21 × 9 cm (8.6 × 8.3 × 3.5 in)
Weight 1.8 kg (4  lb)  *without the AC adapter and power cord