X-CLARITY tissue clearing system with electrophoretic chamber and cooling unit on lab bench for 3D imaging sample preparation | Logos Biosystems

TISSUE CLEARING

X-CLARITY™, instrumentación y reactivos
para la clarificación de tejidos.

X-CLARITY™ es un sitema con reactivos listos para usar desarrollado por Logos Biosystems, facilitando una clarificación electroforética de los tejidos simple, rápida y eficiente.

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El sistema X-CLARITY™ y sus reactivos, estandarizan, simplifican y aceleran cada paso en el proceso de clarificación de tejidos. En el método CLARITY los tejidos son embebidos en matrices de hidrogel, y los lípidos se extraen mediante electroforesis para producir un tejido-hidrogel estable y ópticamente transparente. Este nuevo híbrido, tejido-hidrogel, es químicamente accesible para múltiples rondas de marcaje con anticuerpos y la obtención de imágenes. La cito-estructura nativa se mantiene de forma intacta incluso preservando la fluorescencia endógena de la proteinas para una posterior obtención de imágenes robusta.

01. HYDROGEL INFUSION

X-CLARITY Hydrogel Solution Kit bottle and reagent tube for tissue clearing experiments
X-CLARITY™
Hydrogel Solution Kit
C1310X

02. TISSUE-HYDROGEL HYBRIDIZATION

Diagram showing stabilized hydrogel matrix structure formed inside liver tissue during polymerization
X-CLARITY Polymerization System device with red cover for tissue clearing workflow
X-CLARITY™
Polymerization System
C20001

03. LIPID REMOVAL

Diagram showing electrophoretic tissue clearing process with electric field applied to liver tissue sample
Automated X-CLARITY Tissue Clearing System II for efficient and reproducible tissue clearing experiments
X-CLARITY™
Tissue Clearing System II
C30001
Electrophoretic Tissue Clearing Solution reagent bottle for X-CLARITY tissue clearing system
Electrophoretic
Tissue Clearing Solution
C13001
           

01. Hydrogel Infusion

X-CLARITY™ Hydrogel Solution Kit  C1310X

El kit de solución de hidrogel X-CLARITY™ es una solución de hidrogel pre-testada para una hibridación uniforme y consistente entre tejido e hidrogel. El kit está compuesto por X-CLARITY™ Hydrogel Solution, una solución a base de acrilamida utilizada para crear poliacrilamida, y X-CLARITY™ Polymerization Initiator, un iniciador térmico de radicales libres también conocido como VA-044.

View Hydrogel Solution Kit

02. Tissue-Hydrogel Hybridization

X-CLARITY™ Polymerization System  C20001

El sistema de polimerización X-CLARITY™ es un sistema autónomo y automatizado desarrollado para simplificar la hibridación tejido-hidrogel, un paso crucial para la clarificación óptima de los tejidos. Pueden ser utilizadas placas de varios pocillos o tubos cónicos para un rápido y eficiente procesamiento de muchas muestras a la vez. La interfaz con pantalla táctil permite a los usuarios ajustar la presión de vacío, la temperatura y un temporizador para controlar el proceso de polimerización.

Download Software Update Manual
X-CLARITY Tissue Clearing System device with red lid and front control panel for electrophoretic clearing
Fast tissue clearing icon for accelerated turnaround
Alto rendimiento
(hasta 768 muestras/ run)
Reproducibility icon indicating standardized results
Compatible con distintos
soportes
Easy-to-use icon for simplified operation
Vacío constante y control de temperatura
Safety/quality icon for reliable tissue processing
Polimerización rápida y consistente
Accesorios
X-CLARITY Heat Block accessory designed to hold six 50 mL conical tubes for tissue clearing
X-CLARITY™ Heat Block for 6 x 50 mL tubes
Catalog #: C20002
Quantity : 1 unidad
Holds six 50 mL conical tubes
X-CLARITY Heat Block accessory for flat-bottom plates used in tissue clearing workflows
X-CLARITY™ Heat Block for flat-bottom plates
Catalog #: C20003
Quantity : 1 unidad
Holds one flat-bottom plate
           

03. Lipid Removal

X-CLARITY™ Tissue Clearing System II  C30001

El X-CLARITY™ Tissue Clearing System II es una solución todo en uno y fácil de usar para la clarificación electroforética de tejidos. El diseño único de este dispositivo le permite eliminar los lípidos de los tejidos de manera eficiente mientras preserva la integridad de la muestra. Es increíblemente 8 veces más rápido que la tecnología original, clarificando un cerebro de ratón entero en tan sólo 6 horas. Provisto con una pantalla táctil, los usuarios pueden establecer las condiciones de clarificación de los tejidos. Unos electrodos recubierdos de platino generan un campo eléctrico que acelera la eliminación de lípidos de los tejidos durante el ETC (clarificación electroforética de tejidos). Para mantener una temperatura constante del tampón durante la clarificación, un sistema incorporado de control de temperatura enfría y calienta activamente el tampón. El tampón circula de forma contínua para mantener una capacidad de tampón estable, el control de la temperatura y para eliminar cualquier subproducto de la clarificación de tejidos. Se trata de un sistema avanzado que garantiza una clarificación de tejidos rápida, eficaz y uniforme.

Read X-CLARITY™ Application Note
Sample Holder Guide
X-CLARITY Tissue Clearing System II electrophoretic device with cooling unit and controller for high-resolution tissue clearing
Contrôle précis de la températ
           
X-CLARITY system icon showing active buffer cooling and heating capacity for precise tissue clearing temperature control
Capacidad activa de enfriamiento y calentamiento del tampón
X-CLARITY tissue clearing icon with sensitive accurate temperature sensor for stable clearing process
Sensor de temperatura sensible y preciso
Campo eléctrico uniforme
X-CLARITY electrophoretic tissue clearing platinum plated electrodes icon ensuring uniform electric field
Electrodos recubiertos de platino
Constant current constant voltage CV CC icon for X-CLARITY tissue clearing stable clearing performance
Modos disponibles: corriente constante y voltaje constante
Compatible con múltiples tamaños y tipos de tejido
X-CLARITY icon for active and passive tissue clearing modes compatible with multiple tissue types
Dos modos de clarificación: activa y pasiva
Sample holder icon showing various sizes available for X-CLARITY tissue clearing applications
Soportes de varios tamaños disponibles
Fácil puesta en marcha
Touchscreen interface icon for X-CLARITY tissue clearing system easy operation
Sencillo interfaz con pantalla táctil
Ready-to-use clearing solution reagent bottle icon for X-CLARITY tissue clearing workflow
Solución de clarificación lista para usar
           

Electrophoretic Tissue Clearing Solution  C13001

La solución electroforética de clarificación de tejidos es un tampón premezclado a base de SDS optimizado para su uso con el X-CLARITY™ Tissue Clearing System II.

View Electrophoretic Clearing Solution
Electrophoretic Tissue Clearing Solution bottles for X-CLARITY tissue clearing system optimized SDS buffer
Cerebro completo de ratón Thy1-YFP
Whole Thy1-YFP mouse brain before and after tissue clearing with X-CLARITY system showing preserved fluorescent signal
La señal de Thy1-YFP se conserva y es estale después de la clarificación de tejidos con X-CLARITY™ y sus reactivos.
Corteza cerebral humana (1 mm)
Human cerebral cortex slice before ETC PFA fixation for tissue clearing
Antes de ETC, fijado en PFA (1 semana)
Human cerebral cortex slice after electrophoretic tissue clearing with X-CLARITY system
Después de ETC
Human cerebral cortex slice after refractive index RI matching for transparency improvement
Después de la homogeneización del IR
Courtesy of Hyung Jin Choi, PhD Seoul National University College of Medicine

Marcaje e imagen

Acelere el marcaje de anticuerpos con el NUEVO KIT DeepLabel™ Antibody Labeling Kit.
Discover DeepLabel™
DeepLabel antibody labeling kit for tissue clearing and imaging, Logos Biosystems solution for fast and efficient immunostaining

Why Scientists Love X-CLARITY™ System

Testimonios

(5.0)
Gabriel Bidaux

For you if you want to accelerate your research!
Positive facts: X-CLARITY system is easy to use, devoted to aqueous-based method for organ clearing. It helps clearing several organs at once and in a short period of time. Works well on a lot of different mouse organs or even pieces of primates and humans organs. Logos Biosystems after Sales are fast and always find a compromise as a solution to our rare issues. Minus: expensive systems in comparison to passive clearing methods. For you if you want to accelerate your research!

INSERM UMR 1060

(5.0)
Tori N

Great results, easy to use!
This product was effective at clearing my tissue samples. It cleared whole brains, hearts, stomach, kidneys, livers, etc. The user manual was easy to read and easy to follow. There is little attention and maintenance required while the system in running.

Clemson University

Read Peer Reviews

Referencias del X-CLARITY™

Citas

Influenza causes alterations to oligodendrocyte-specific transcripts and the myelin lipidome in the adult mouse central nervous system.

2021. Louie A, Kim J, Soto-Diaz K, Dibaeinia P, Koito H, Sinha S, Nowak R, Das A, Steelman A. Research Squre https://doi.org/10.21203/rs.3.rs-310861/v1.

Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models.

2021. Chourrout M, Rositi H, Ong E, Hubert V, Paccalet A, Foucault L, Autret A, Fayard B, Olivier C, Bolbos R, Peyrin F, Crola-da-Silva C, Meyronet D, Raineteau O, Elleaume H, Brun E, Chauveau F, Wiart M. bioRxiv 2021.03.24.436852.

Novel insights into the electrophysiology of murine cardiac macrophages: relevance of voltage-gated potassium channels.

2021. Simon-Chica A, Fernández MC, Wülfers EM, Lother A, Hilgendorf I, Seemann G, Ravens U, Kohl P, Schneider-Warme F. Cardiovascular Research cvab126.

3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice.

2020. Ded L, Hwang JY, Miki K, Shi HF, Chung JJ. Elife 9:e62043.

Maximizing lentiviral vector gene transfer in the CNS.

2020. Humbel M, Ramosaj M, Zimmer V, Regio S, Aeby L, Moser S, Boizot A, Sipion M, Rey M, Déglon N. Gene Therapy 10.1038/s41434-020-0172-6.

View Cited Works

Recursos

Descargas

DeepLabel™ Antibody Staining Kit
Folleto

2022-04-07 | 458.10 KB

Download
X-CLARITY™ Systems and Reagents for Tissue Clearing
Brochure

2022-04-07 | 962KB

Download
Logos Biosystems Products
Brochure

2022-04-07 | 2.14 MB

Download
The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
Póster

2018-11-26 | 1.25 MB

Download
X-CLARITY™ Systems and Reagents for Tissue Clearing
Folleto

2018-05-08 | 553 KB

Download
X-CLARITY™ Tissue Clearing System
Guía de inicio rápido

2018-05-08 | 668.54 KB

Download
X-CLARITY™ Tissue Clearing System II
Guía de instalación

2018-05-08 | 841.04 KB

Download
ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
Documentación del producto

2018-04-17 | 2.6 MB

Download
An automated and high-throughput polymerization solution for downstream tissue clearing: the X-CLARITY™ Polymerization System
Documentación del producto

2018-04-17 | 1.4 MB

Download

2022-04-21 | 548.40 KB

Download
Galería de imágenes
X-CLARITY™ cleared whole mouse brain sample on grid background for high-resolution 3D tissue imaging
Mouse brain | X-CLARITY™
X-CLARITY™ tissue clearing of mouse brain showing optical transparency and structural preservation
Mouse brain | X-CLARITY™
X-CLARITY™ cleared mouse brain hemisphere prepared for detailed microscopy and analysis
Mouse brain hemisphere | X-CLARITY™
X-CLARITY™ tissue cleared 2 mm mouse brain slices demonstrating uniform transparency
Mouse brain - 2 mm slices | X-CLARITY™
X-CLARITY™ cleared mouse brain slices (1, 2, 3 mm) comparison before and after tissue clearing
Mouse brain - 1,2,3 mm slices | X-CLARITY™
X-CLARITY™ cleared mouse brain slice with fluorescent labeling from Korea Brain Research Institute
Mouse brain slice | X-CLARITY™
Courtesy of Youngshik Choe, PhD | Korea Brain Research Institute
Explore Entire Cell Image
Información para pedidos
Cat # Product Qty
C20001 X-CLARITY™ Polymerization System 1 unit
C20002 X-CLARITY™ Heat Block for 6 x 50 mL tubes 1 unit
C20003 X-CLARITY™ Heat Block for flat-bottom plates 1 unit
C1310X X-CLARITY™ Hydrogel Solution Kit
– X-CLARITY™ Hydrogel Solution (1 x 1 L)
– X-CLARITY™ Polymerization Initiator (1 x 2.5 g)
1 kit
C30001 X-CLARITY™ Tissue Clearing System II 1 unit
C12010 Holder for 36 Mouse Brain Slices, 1.5 Φ 1 set
C12020 Holder for 36 Mouse Brain Slices, 0.6 Φ 1 set
C12011 Holder for 6 Slices, 1.5 Φ 1 set
C12021 Holder for 6 Slices, 0.6 Φ 1 set
C12012 Holder for 1 Sample, 1.5 Φ 1 set
C12022 Holder for 1 Sample, 0.6 Φ 1 set
C12013 Holder for 6 Mouse Brains, 1.5 Φ 1 set
C12023 Holder for 6 Mouse Brains, 0.6 Φ 1 set
C12014 Holder for 48 Samples, 1.5 Φ 1 set
C12024 Holder for 48 Samples, 0.6 Φ 1 set
C12015 Holder for 192 Samples, 1.5 Φ 1 set
C12025 Holder for 192 Samples, 0.6 Φ 1 set
C12001 Tissue Container (Whole Mouse Brain), 20 units 1 box
C12002 Container Holder for 1 Tissue Container 1 unit
C12004 Mouse Brain Slice Holder (Chamber Height: 4 mm) 1 unit
C12007 Whole Rat Brain Holder 1 unit
C13001 Electrophoretic Tissue Clearing Solution 12 x 1 L
C13101 X-CLARITY™ Mounting Solution 1 x 25 mL
C13102 X-CLARITY™ Mounting Solution Value Pack 10 x 25 mL
C13107 X-CLARITY™ Mounting Solution Bulk Pack 20 x 25 mL
C33001 DeepLabel™ Antibody Staining Kit
– DeepLabel™ Solution A (1 x 25 mL)
– DeepLabel™ Solution B (2 x 25 mL)
– DeepLabel™ Washing Buffer (1 x 250 mL)
1 kit
Especificaciones

 

X-CLARITY™ Polymerization System

 

Display 5”  TFT LCD
Temperature Range RT – 60 °C
Temperature Accuracy ±0.3 °C
Vacuum Range -90 – 0 kPa
Power Consumption 312 W
Applicable Power AC 100-240 V, 50/60 Hz
Dimensions (W x D x H) Exterior: 332 x 430 x 222 mm
Interior: 307 x 137 x 140 mm
Weight 28 kg

 

X-CLARITY™ ETC Chamber

 

Instrument type Electrophoretic chamber
External dimensions (W x D x H) 176 mm x 128 mm x 154 mm
Internal dimensions (W x D x H) 57 mm x 30 mm x 93 mm
Weight 2.8 kg

 

X-CLARITY™ ETC Control Tower

 

Instrument type Power supply, temperature control,
and buffer circulation device
User interface 5 “ TFT LCD touchscreen
Power supply modes Constant current or constant voltage
Current & voltage range 0.2-1.5 A, 5-70 V
Temperature range 30-60 ° C
Pump speed range 50-200 rpm
Electrical requirements AC 100-240 V, 50/60 Hz
Power consumption 500 W (including the X-CLARITY™ ETC Chamber)
Dimensions (W x D x H) 205 mm x 430 mm x 370 mm
Weight 20 kg