Great for primary cells.
We use the LUNA-FL cell counter for analysis of mouse tissue preparations and primary cell cultures. Our samples include: lung homogenates, bronchoalveolar lavage fluid, T cells culture, etc. Acridine orange/propidium iodide dual staining is particularly beneficial for evaluation of samples contaminated with red blood cells. This approach is fast (analysis conducted immediately after addition of dyes) and more precise than methods utilizing trypan blue staining. Wide detection range and availability of re-usable slides are another advantages of the LUNA-FL. Major disadvantages include: no internal memory, only one USB port – precluding simultaneous use of pendrive (for saving results) and printer, long-lasting saving of results, lack of automatic focusing.