X-CLARITY tissue clearing system with electrophoretic chamber and cooling unit on lab bench for 3D imaging sample preparation | Logos Biosystems

TISSUE CLEARING

X-CLARITY™ 組織透明化用システム&試薬

X-CLARITY™は、電気泳動による組織透明化をシンプル・迅速・効率的に行うためにLogos Biosystemsが開発した、システムとすぐに使える試薬のシリーズです。

無料デモをリクエスト

X-CLARITY™のシステムおよび試薬は、組織透明化プロセスの各ステップを標準化・簡素化・加速化します。 CLARITY法を用いることで、保存された組織をハイドロゲルマトリックスに埋め込み、電気泳動により脂質を能動的に除去し、**安定かつ光学的に透明な「組織-ハイドロゲルハイブリッド」**を作製します。 この状態は化学的にアクセス可能で、抗体染色やイメージングを複数回行うことが可能です。 さらに、細胞の微細構造(cytoarchitecture)や内在性蛍光タンパク質も保持され、後続の蛍光イメージングにも適しています。

01. HYDROGEL INFUSION

X-CLARITY Hydrogel Solution Kit bottle and reagent tube for tissue clearing experiments
X-CLARITY™
Hydrogel Solution Kit
C1310X

02. TISSUE-HYDROGEL HYBRIDIZATION

Diagram showing stabilized hydrogel matrix structure formed inside liver tissue during polymerization
X-CLARITY Polymerization System device with red cover for tissue clearing workflow
X-CLARITY™
Polymerization System
C20001

03. LIPID REMOVAL

Diagram showing electrophoretic tissue clearing process with electric field applied to liver tissue sample
Automated X-CLARITY Tissue Clearing System II for efficient and reproducible tissue clearing experiments
X-CLARITY™
Tissue Clearing System II
C30001
Electrophoretic Tissue Clearing Solution reagent bottle for X-CLARITY tissue clearing system
Electrophoretic
Tissue Clearing Solution
C13001
           

01. Hydrogel Infusion

X-CLARITY™ Hydrogel Solution Kit  C1310X

X-CLARITY™ ハイドロゲル溶液キットは、均一で一貫性のある組織-ハイドロゲルハイブリッドを形成するために最適化された、事前テスト済みのハイドロゲル溶液です。
このキットは以下の2つの成分で構成されています: X-CLARITY™ ハイドロゲル溶液:ポリアクリルアミド形成のためのアクリルアミドベースの溶液 X-CLARITY™ 重合開始剤:熱によるフリーラジカル反応を促す重合開始剤(VA-044)

View Hydrogel Solution Kit

02. Tissue-Hydrogel Hybridization

X-CLARITY™ Polymerization System  C20001

X-CLARITY™ 重合システムは、組織とハイドロゲルのハイブリッド化を自動化し、簡便化するために開発されたスタンドアロン型システムです。
このステップは、透明化を最適化するための重要な工程です。 複数のサンプルをマルチウェルプレートまたはコニカルチューブにセットできるため、迅速かつ効率的なハイスループット処理が可能です。
ユーザーは、シンプルなタッチスクリーンインターフェースを通じて、真空強度・温度・タイマーを調整し、重合プロセスを制御できます。

Download Software Update Manual
X-CLARITY Tissue Clearing System device with red lid and front control panel for electrophoretic clearing
Fast tissue clearing icon for accelerated turnaround
ハイスループット対応
(最大768サンプル/1ラン)
Reproducibility icon indicating standardized results
さまざまな容器に対応
Easy-to-use icon for simplified operation
安定した真空および温度制御
Safety/quality icon for reliable tissue processing
迅速かつ一貫した重合処理
アクセサリー
X-CLARITY Heat Block accessory designed to hold six 50 mL conical tubes for tissue clearing
X-CLARITY™ Heat Block for 6 x 50 mL tubes
Catalog #: C20002
Quantity : 容量
Holds six 50 mL conical tubes
X-CLARITY Heat Block accessory for flat-bottom plates used in tissue clearing workflows
X-CLARITY™ Heat Block for flat-bottom plates
Catalog #: C20003
Quantity : 容量
Holds one flat-bottom plate
           

03. Lipid Removal

X-CLARITY™ Tissue Clearing System II  C30001

X-CLARITY™ Tissue Clearing System II は、電気泳動による組織透明化のための、オールインワンで使いやすいソリューションです。 その独自の設計により、試料の構造的完全性を保持しながら、組織からの脂質除去を加速します。 マウス全脳をわずか6時間で透明化でき、これは従来技術と比べて驚異的な8倍の速さです。 ユーザーはシンプルで直感的なタッチスクリーンインターフェースを通じて、組織透明化の条件を設定できます。 ETC(電気泳動組織透明化)モードでは、白金コーティングされた電極が電場を発生させ、組織からの脂質除去を非常に効率的に加速します。 内蔵の温度制御システムは、バッファーを積極的に加温・冷却し、透明化中の一定のバッファー温度を維持します。 バッファーは常時循環され、バッファー容量、温度制御、および透明化副産物の除去を安定して実現します。 この高度なシステムは、効率的で迅速かつ一貫性のある組織透明化を可能にします。

Read X-CLARITY™ Application Note
Sample Holder Guide
X-CLARITY Tissue Clearing System II electrophoretic device with cooling unit and controller for high-resolution tissue clearing
精密な温度制御
           
X-CLARITY system icon showing active buffer cooling and heating capacity for precise tissue clearing temperature control
バッファーの能動的な冷却・加温機能
X-CLARITY tissue clearing icon with sensitive accurate temperature sensor for stable clearing process
高感度かつ高精度な温度センサー
均一な電場
X-CLARITY electrophoretic tissue clearing platinum plated electrodes icon ensuring uniform electric field
白金メッキ電極
Constant current constant voltage CV CC icon for X-CLARITY tissue clearing stable clearing performance
定電流モードおよび定電圧モード
複数の組織タイプおよびサイズに対応
X-CLARITY icon for active and passive tissue clearing modes compatible with multiple tissue types
アクティブとパッシブ の クリアリングモード
Sample holder icon showing various sizes available for X-CLARITY tissue clearing applications
様々なサイズのホルダーをご用意
ユーザーフレンドリーなセットアップ
Touchscreen interface icon for X-CLARITY tissue clearing system easy operation
シンプルなタッチスクリーンインターフェース
Ready-to-use clearing solution reagent bottle icon for X-CLARITY tissue clearing workflow
すぐに使える洗浄液
           

Electrophoretic Tissue Clearing Solution  C13001

電気泳動組織透明化溶液は、X-CLARITY™ 組織透明化システム II で使用するために最適化された、事前に混合された SDS ベースのバッファーです。

View Electrophoretic Clearing Solution
Electrophoretic Tissue Clearing Solution bottles for X-CLARITY tissue clearing system optimized SDS buffer
Thy1-YFPマウス脳(whole)
Whole Thy1-YFP mouse brain before and after tissue clearing with X-CLARITY system showing preserved fluorescent signal
Thy1-YFP シグナルは、X-CLARITY™ システムと試薬による組織洗浄後も保存され、鮮明です。
ヒト大脳皮質(1 mm)
Human cerebral cortex slice before ETC PFA fixation for tissue clearing
ETC前 PFA固定(1週間)
Human cerebral cortex slice after electrophoretic tissue clearing with X-CLARITY system
ETC後
Human cerebral cortex slice after refractive index RI matching for transparency improvement
RIマッチング後
Courtesy of Hyung Jin Choi, PhD Seoul National University College of Medicine

ラベリングとイメージング

全てが新しい DeepLabel™ 抗体ラベリング キットは、抗体のラベリングを加速します。
Discover DeepLabel™
DeepLabel antibody labeling kit for tissue clearing and imaging, Logos Biosystems solution for fast and efficient immunostaining

Why Scientists Love X-CLARITY™ System

お客様の声

(5.0)
Gabriel Bidaux

For you if you want to accelerate your research!
Positive facts: X-CLARITY system is easy to use, devoted to aqueous-based method for organ clearing. It helps clearing several organs at once and in a short period of time. Works well on a lot of different mouse organs or even pieces of primates and humans organs. Logos Biosystems after Sales are fast and always find a compromise as a solution to our rare issues. Minus: expensive systems in comparison to passive clearing methods. For you if you want to accelerate your research!

INSERM UMR 1060

(5.0)
Tori N

Great results, easy to use!
This product was effective at clearing my tissue samples. It cleared whole brains, hearts, stomach, kidneys, livers, etc. The user manual was easy to read and easy to follow. There is little attention and maintenance required while the system in running.

Clemson University

Read Peer Reviews

X-CLARITY™ の引用実績

引用

Influenza causes alterations to oligodendrocyte-specific transcripts and the myelin lipidome in the adult mouse central nervous system.

2021. Louie A, Kim J, Soto-Diaz K, Dibaeinia P, Koito H, Sinha S, Nowak R, Das A, Steelman A. Research Squre https://doi.org/10.21203/rs.3.rs-310861/v1.

Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models.

2021. Chourrout M, Rositi H, Ong E, Hubert V, Paccalet A, Foucault L, Autret A, Fayard B, Olivier C, Bolbos R, Peyrin F, Crola-da-Silva C, Meyronet D, Raineteau O, Elleaume H, Brun E, Chauveau F, Wiart M. bioRxiv 2021.03.24.436852.

Novel insights into the electrophysiology of murine cardiac macrophages: relevance of voltage-gated potassium channels.

2021. Simon-Chica A, Fernández MC, Wülfers EM, Lother A, Hilgendorf I, Seemann G, Ravens U, Kohl P, Schneider-Warme F. Cardiovascular Research cvab126.

3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice.

2020. Ded L, Hwang JY, Miki K, Shi HF, Chung JJ. Elife 9:e62043.

Maximizing lentiviral vector gene transfer in the CNS.

2020. Humbel M, Ramosaj M, Zimmer V, Regio S, Aeby L, Moser S, Boizot A, Sipion M, Rey M, Déglon N. Gene Therapy 10.1038/s41434-020-0172-6.

View Cited Works

リソース

ダウンロード

DeepLabel™ Antibody Staining Kit
フライヤー

2022-04-07 | 458.10 KB

Download
X-CLARITY™ Systems and Reagents for Tissue Clearing
Brochure

2022-04-07 | 962KB

Download
Logos Biosystems Products
Brochure

2022-04-07 | 2.14 MB

Download
The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
ポスター

2018-11-26 | 1.25 MB

Download
X-CLARITY™ Systems and Reagents for Tissue Clearing
フライヤー

2018-05-08 | 553 KB

Download
X-CLARITY™ ティッシュクリアリングシステム
クイックスタートガイド

2018-05-08 | 668.54 KB

Download
X-CLARITY™ Tissue Clearing System II
インストールガイド

2018-05-08 | 841.04 KB

Download
ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging
製品資料

2018-04-17 | 2.6 MB

Download
An automated and high-throughput polymerization solution for downstream tissue clearing: the X-CLARITY™ Polymerization System
製品資料

2018-04-17 | 1.4 MB

Download
テクニカルノート

2022-04-21 | 548.40 KB

Download
画像ギャラリー
X-CLARITY™ cleared whole mouse brain sample on grid background for high-resolution 3D tissue imaging
Mouse brain | X-CLARITY™
X-CLARITY™ tissue clearing of mouse brain showing optical transparency and structural preservation
Mouse brain | X-CLARITY™
X-CLARITY™ cleared mouse brain hemisphere prepared for detailed microscopy and analysis
Mouse brain hemisphere | X-CLARITY™
X-CLARITY™ tissue cleared 2 mm mouse brain slices demonstrating uniform transparency
Mouse brain - 2 mm slices | X-CLARITY™
X-CLARITY™ cleared mouse brain slices (1, 2, 3 mm) comparison before and after tissue clearing
Mouse brain - 1,2,3 mm slices | X-CLARITY™
X-CLARITY™ cleared mouse brain slice with fluorescent labeling from Korea Brain Research Institute
Mouse brain slice | X-CLARITY™
Courtesy of Youngshik Choe, PhD | Korea Brain Research Institute
Explore Entire Cell Image
注文情報
Cat # Product Qty
C20001 X-CLARITY™ Polymerization System 1 unit
C20002 X-CLARITY™ Heat Block for 6 x 50 mL tubes 1 unit
C20003 X-CLARITY™ Heat Block for flat-bottom plates 1 unit
C1310X X-CLARITY™ Hydrogel Solution Kit
– X-CLARITY™ Hydrogel Solution (1 x 1 L)
– X-CLARITY™ Polymerization Initiator (1 x 2.5 g)
1 kit
C30001 X-CLARITY™ Tissue Clearing System II 1 unit
C12010 Holder for 36 Mouse Brain Slices, 1.5 Φ 1 set
C12020 Holder for 36 Mouse Brain Slices, 0.6 Φ 1 set
C12011 Holder for 6 Slices, 1.5 Φ 1 set
C12021 Holder for 6 Slices, 0.6 Φ 1 set
C12012 Holder for 1 Sample, 1.5 Φ 1 set
C12022 Holder for 1 Sample, 0.6 Φ 1 set
C12013 Holder for 6 Mouse Brains, 1.5 Φ 1 set
C12023 Holder for 6 Mouse Brains, 0.6 Φ 1 set
C12014 Holder for 48 Samples, 1.5 Φ 1 set
C12024 Holder for 48 Samples, 0.6 Φ 1 set
C12015 Holder for 192 Samples, 1.5 Φ 1 set
C12025 Holder for 192 Samples, 0.6 Φ 1 set
C12001 Tissue Container (Whole Mouse Brain), 20 units 1 box
C12002 Container Holder for 1 Tissue Container 1 unit
C12004 Mouse Brain Slice Holder (Chamber Height: 4 mm) 1 unit
C12007 Whole Rat Brain Holder 1 unit
C13001 Electrophoretic Tissue Clearing Solution 12 x 1 L
C13101 X-CLARITY™ Mounting Solution 1 x 25 mL
C13102 X-CLARITY™ Mounting Solution Value Pack 10 x 25 mL
C13107 X-CLARITY™ Mounting Solution Bulk Pack 20 x 25 mL
C33001 DeepLabel™ Antibody Staining Kit
– DeepLabel™ Solution A (1 x 25 mL)
– DeepLabel™ Solution B (2 x 25 mL)
– DeepLabel™ Washing Buffer (1 x 250 mL)
1 kit
仕様

 

X-CLARITY™ Polymerization System

 

Display 5”  TFT LCD
Temperature Range RT – 60 °C
Temperature Accuracy ±0.3 °C
Vacuum Range -90 – 0 kPa
Power Consumption 312 W
Applicable Power AC 100-240 V, 50/60 Hz
Dimensions (W x D x H) Exterior: 332 x 430 x 222 mm
Interior: 307 x 137 x 140 mm
Weight 28 kg

 

X-CLARITY™ ETC Chamber

 

Instrument type Electrophoretic chamber
External dimensions (W x D x H) 176 mm x 128 mm x 154 mm
Internal dimensions (W x D x H) 57 mm x 30 mm x 93 mm
Weight 2.8 kg

 

X-CLARITY™ ETC Control Tower

 

Instrument type Power supply, temperature control,
and buffer circulation device
User interface 5 “ TFT LCD touchscreen
Power supply modes Constant current or constant voltage
Current & voltage range 0.2-1.5 A, 5-70 V
Temperature range 30-60 ° C
Pump speed range 50-200 rpm
Electrical requirements AC 100-240 V, 50/60 Hz
Power consumption 500 W (including the X-CLARITY™ ETC Chamber)
Dimensions (W x D x H) 205 mm x 430 mm x 370 mm
Weight 20 kg