Incomparable cell size measurement with the LUNA-FX7™
#Cell size determination #cell health and quality #bioresearch #biomanufacturing
#precise size gaiting before and after counting #accurate and reliable size measurement
Here, we demonstrate the accuracy and reliability of the LUNA-FX7™ in measuring cell size and its precise size gating ability. For evaluating the accuracy in determining cell size, six different NIST Traceable Particle Size Standards and two cell lines, AsPC-1 (Pancreatic carcinoma) and DLD-1 (Colon carcinoma), were counted in the Brightfield Total Cell Counting mode.
- Figure 1. Size determination of NIST Traceable Particle Size Standards. (A-F) The images show well-tagged particles, indicating the precise measurement of particle diameter size. (G) The table shows measured particle sizes and the size measuring accuracy of the LUNA-FX7™ and a competitor's counter.
- Figure 2. Specific size gating in the LUNA-FX7™. The tagged images and the histograms show that the target size particles, indicated in orange, are intensively, selectively, and exclusively detected by gating.
- Figure 3. Size determination of NIST Traceable Particle Size Standards. (A-F) The images show well-tagged particles, indicating the Precise size determination. (A) The image shows well-tagged particles in various sizes. (B-C) The histogram indicates the exact distribution of the NIST particles by size.
- Figure 4. Cell counts and size determinations of AsPC-1 and DLD-1 cells. AsPC-1 (A-C) and DLD-1 (D-F) cell lines were applied to confirm the counting performance in various sizes mixed.
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