Rudolph Tannenberg

Robust results. Quicker than manual counting but far more expensive.
I was able to obtain good agreement between the PI channel of the Luna FL and my own manual haemocytometer counting of nuclei stained with DAPI at a fraction of the time. One caveat would be false ‘viability’ given from background staining of AO on tissue debris but it could be useful in determining the level of debris from the isolation.