The X-CLARITY™ Hydrogel Solution Kit is a pre-tested hydrogel solution for uniform and consistent tissue-hydrogel hybridization. The kit is composed of the X-CLARITY™ Hydrogel Solution and the X-CLARITY™ Polymerization Initiator.
X-CLARITY™ Hydrogel Solution is an acrylamide-based solution used to create polyacrylamide, a chemically inert and electrically neutral gel matrix. The X-CLARITY™ Hydrogel Solution contains no bis-acrylamide or paraformaldehyde.
The X-CLARITY™ Polymerization Initiator is a thermal free radical initiator also known as VA-044. VA-044 is relatively stable at room temperature but undergoes rapid hemolytic decomposition when heated in aqueous solution, releasing cationic free radicals that initiate the polymerization of hydrogel monomers
References
1. Lee, E et al. ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3 dimensional (3D) imaging. Sci Rep 6, 18631 (2016)
2. Yang, B. et al. Single-cell phenotyping within transparent intact tissue through whole-body clearing. Cell 158, 945–958 (2014)
3. Chung, K. et al. Structural and molecular interrogation of intact biological systems. Nature 497, 332–337 (2013)
| X-CLARITYTM Hydrogel Solution | |
| Product identity | 4% acrylamide solution |
| Molecular formula | C3H5NO |
| Molecular weight | 71.08 g/mol |
| Appearance | Clear liquid |
| Storage | 4°C in the dark |
| X-CLARITYTM Polymerization Initiator | |
| Molecular formula | C12H22N6· 2HCl |
| Molecular weight | 323.33 g/mol |
| Appearance | Pale yellow powder |
| 10-hour half-life | 44°C (in water) |
| Storage | 4°C |
| Cat # | Description | Qty |
| C1310X | X-CLARITY™ Hydrogel Solution Kit | 1 box |
| – X-CLARITY™ Hydrogel Solution (1 L) | ||
| – X-CLARITY™ Polymerization Initiator (2.5 g) | ||
| C13103 | X-CLARITY™ Hydrogel Solution | 1 L |
| C13104 | X-CLARITY™ Polymerization Initiator | 2.5 g |
