LUNA-STEM™は、脂肪由来幹細胞およびSVF（間質血管分画）サンプルから、生細胞、有核死細胞、無核細胞を正確かつ一貫してカウントし、後続の工程に必要なデータを提供します。
幹細胞や脂肪組織由来のSVFなどの組織由来細胞を使用する際には、細胞濃度と生存率を把握することが、後続工程の品質を確保・管理するうえで極めて重要です。しかし、組織由来サンプルには赤血球や非細胞性デブリが混在していることが多く、細胞カウントは時間がかかり、主観的で誤差の多い作業となりがちです。 LUNA-STEM™は、デュアル蛍光および明視野光学系を搭載しており、有核の生細胞および死細胞を無核細胞やデブリから明確に区別します。サンプルに核酸染色を施すことで、有核単核細胞の濃度と生存率を正確に測定。無核細胞もデブリと区別され、同時にカウントされます。 わずか30秒で、LUNA-STEM™は、総細胞濃度、有核細胞濃度、無核細胞濃度、生存率、平均細胞サイズを自動で算出します。結果は高解像度画像として表示され、生細胞には緑色の円、死細胞には赤色の円が付けられ、視覚的にデータを確認できます。
Consistent and accurate day after day!The LUNA-STEM Counter is very quick, effective, and accurate. We use the LUNA-STEM Counter on a daily basis and have run into no troubles for 2 years now. In addition we are a consumer of the LUNA-STEM Slides and AOPI Fluorescent dye, both of which are very inexpensive and extremely obtainable - with help from outstanding customer service. Definitely worth the money.
Best cell counting. Reliable and easy to use.We use it to count mononucleated cells. Very useful and easy to use. Perfect for our daily use.
Hospital de la Santa Creu i Sant Pau
Methods and strategies for procurement, isolation, characterization, and assessment of senescence of human mesenchymal stem cells from adipose tissue.
2019. Gaur M, Dobke M, Lunyak VV. Methods in Molecular Biology.
Serial injections of cryopreserved fat at −196°c for tissue rejuvenation, scar treatment, and volume augmentation.
2018. Ohashi M, Chiba A, Nakai H, Fukuda E, Higuchi T. Plastic and Reconstructive Surgery-Global Open, 6(5): e1742.
Comparison of two automated cell counters for enumeration and viability of mesenchymal stem cells for clinical cellular therapy trials
2018. Radel DJ, Madde P, Dietz A. Cytotherapy, 20(5): S69–S70.
Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia.
2016. Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Cell transplantation. 25(9): 1623-1633.
The safety and efficacy of cell-assisted fat grafting to traditional fat grafting in the anterior mid-face: an indirect assessment by 3D imaging.
2015. Sasaki GH. Aesthetic Plastic Surgery, 39(6):833-46.
| Cat # | Product | Qty |
|---|---|---|
| L30001 | LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF |
1 unit |
| L12005 | PhotonSlide™, 50 Slides | 1 box |
| L12006 | PhotonSlide™, 500 Slides | 10 boxes |
| L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
| L12011 | LUNA™ Reusable Slide | 1 unit |
| L12012 | LUNA™ Reusable Slides | 2 units |
| L12014 | LUNA™ Reusable Slide Coverslips | 10 units |
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
| F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
| F23212 | Cell Dilution Buffer | 5 x 20 mL |
| F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
| L34003 | LUNA-STEM™ IQ/OQ Protocol | 1 copy |
| P10001 | LUNA™ Printer I | 1 unit |
| P12001 | LUNA™ Printer I Paper (Thermal, 275 prints/roll, 6rolls) | 3 x 2 rolls |
| Sample Volume | 10 μl |
| Cell Counting Time | 30-60 sec (depending on sample conditions) |
| Cell Concentration Range | 5×104 – 1×107 cells/mL (optimal range) |
| Cell Size Range | Detectable Range: 1 – 90 μm Optimal Range: 5 – 60 μm |
| Excitation wavelength | 470 ± 25 nm |
| Emission wavelength | 530 ± 25 nm, 600 nm (LP) |
| Light Source | LED |
| Image Resolution | 5 MP |
| LCD Display | 7 inches (800 x 480 pixels) |
| Dimensions (W×D×H) | 22 × 21 × 9 cm (8.6 × 8.3 × 3.5 in) |
| Weight | 1.8 kg (4 lb) *without the AC adapter |
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