Tissue Clearing

2021-01-27



Duration Time: 53:43

 

Light sheet microscopy has become a key imaging method for plant biology. With new clearing methods available, it is now even easier to study plant development. However, more research studies are looking to apply light sheet microscopy techniques to focus on root imaging, which requires new methods for sample mounting and imaging to be developed.

In the second part of our webinar series, Dr. Tim Steppe of Logos Biosystems will discuss key considerations for clearing plant samples using the X-CLARITY™ Tissue Clearing System for in vitro imaging. Mathieu Cortes will then discuss how to use the Alpha3 light sheet microscope to extend the scope of plant imaging, including the dynamics of cortical microtubules and aerial organs of Arabidopsis thaliana.

In this webinar, you’ll discover:
∙ An imaging pipeline for quantitative analysis of cortical microtubules dynamics at high temporal resolution over a large volume.
∙ An efficient in vivo imaging protocol for aerial organs, which is challenging for conventional light sheet microscopy. Due to the sample flexibility of the Alpha3 system, large samples and even multiple samples can be easily mounted for live imaging experiments.

Speakers

  • Tim Steppe, PhD
  • Tim Steppe, PhD, Application Specialist, Logos Biosystems
    Tim Steppe is an Application Specialist with Logos Biosystems. He has a PhD from the University of North Carolina at Chapel Hill in marine sciences and 15 years of industry experience. He has held various roles from technical support to marketing, all with a focus on customer education.
  • Matthieu Cortes
  • Matthieu Cortes, PhD Candidate, Ecole Normale Supérieure de Lyon
    Matthieu obtained his master's degree in cell and molecular biology while specializing in bioimaging at the University Claude Bernard Lyon. He is a PhD candidate at the Laboratory of Plant Reproduction and Development in the Mechanotransduction in Development research team headed by Olivier Hamant at Ecole Normale Supérieure de Lyon.
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