X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS)
modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460
at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching
and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue
samples.
App Note
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LABELING & IMAGING
DeepLabel™ Antibody Staining Kit for Cleared Tissues
DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging.
ANTIBODY LABELING FOR CLEARED TISSUES
Clearing methods allow light to penetrate samples for imaging, although the method used can affect tissue permeability to molecular probes. Conventional labeling protocols involve the slow diffusion of probes into thin sample sections, which translates to a time-consuming impracticality when applied to thicker samples. The slow progression of diffusion can also lead to uneven staining, with higher, nonspecific binding on the outside of a thicker sample. The new DeepLabel™ Antibody Staining Kit from Logos Biosystems overcomes these obstacles. Optimized for thick, cleared tissues, DeepLabel™ increases tissue permeability and enhances antigen-antibody binding so that antibody labeling of cleared tissues can happen rapidly and efficiently.
ANTIBODY LABELING
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DeepLabel™ Antibody Staining Kit C33001DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues (compatible with CLARITY, PACT, iDISCO, CUBIC, etc).SFN 2018 POSTER
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Compatible with
multiple clearing methods -
Simple protocol
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Vibrant imaging at
subcellular resolution -
Site-specific labeling
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Homogenous antibody distribution
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2.6 X greater signal-to-background
The effect of DeepLabel™ on anti-Collagen IV penetration into mouse brain tissues
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Without DeepLabel
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With DeepLabel
Adult mouse brain tissue stained with anti-Collagen IV (red) and imaged with a confocal microscope.
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Adult mouse brain stained using DeepLabel with anti-β-Amyloid (yellow), anti-Iba1 (red), and anti-SMA (purple).
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Whole adult Drosophila brain stained using DeepLabel with anti-GFP (green) and anti-TH (red).
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Whole adult mouse brain stained using DeepLabel with anti-TH (red).
DEEPLABEL
Request CoA >
| Cat # | Product | Qty | MSDS | Product Insert |
|---|---|---|---|---|
| C33001 | DeepLabel™ Antibody Staining Kit | 1 kit |
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| C33002 | DeepLabel™ Solution A | 25 mL |
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| C33003 | DeepLabel™ Solution B | 25 mL |
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| C33004 | DeepLabel™ Washing Buffer | 250 mL |
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IMAGING
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X-CLARITY™ Mounting Solution C13101 | C13102 | C13107
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RI = 1.46 at 25°C
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Minimizes photobleaching
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Preserves fluorescence
Human cerebral cortex (1 mm)
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Before ETC
PFA-fixed (1 week) -
After ETC -
After RI matching
Courtesy of Hyung Jin Choi, PhD
Seoul National University College of Medicine
Seoul National University College of Medicine
Volumetric images of auditory neurons in a zebra finch brain (3 mm)
Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution. Auditory neurons of a zebra finch were labeled with GFP bilaterally via injection of a viral vector encoding GFP, stained with propidium iodide (PI) to visualize sample structure, and imaged with a light sheet microscope.
Courtesy of Makoto Araki, PhD and Yuichi Morohashi, PhD
Okinawa Institute of Science and Technology
Okinawa Institute of Science and Technology
MOUNTING SOLUTION
Request CoA >
| Cat # | Product | Qty | MSDS | Product Insert |
|---|---|---|---|---|
| C13101 | X-CLARITY™ Mounting Solution | 1 x 25 mL |
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| C13102 | X-CLARITY™ Mounting Solution Value Pack | 10 x 25 mL |
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WHERE THE DEEPLABEL™ HAS BEEN CITED
Citations
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Multiplex immunolabeling and imaging of functionally essential kidney structures in X-CLARITY-cleared tissue.
2019. Mochama P, Tyshynsky R, Sanders MA. Microscopy and Microanalysis 25(Supplement S2): 1260-1261.
Resources
Downloads
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DeepLabel™ Antibody Staining KitFlyer
2022-04-07 | 458.10 KB -
X-CLARITY™ Systems and Reagents for Tissue Clearing
Brochure
2022-04-07 | 962KB -
Logos Biosystems Products
Brochure
2022-04-07 | 2.14 MB -
The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
Poster
2018-11-26 | 1.25 MB
ORDERING INFORMATION
| Cat # | Product | Qty |
|---|---|---|
| C33001 | DeepLabel™ Antibody Staining Kit - DeepLabel™ Solution A (1 x 25 mL) - DeepLabel™ Solution B (2 x 25 mL) - DeepLabel™ Washing Buffer (1 x 250 mL) |
1 kit |
| C33002 | DeepLabel™ Solution A | 1 x 25 mL |
| C33003 | DeepLabel™ Solution B | 2 x 25 mL |
| C33004 | DeepLabel™ Washing Buffer | 1 x 250 mL |
| C13101 | X-CLARITY™ Mounting Solution | 1 x 25 mL |
| C13102 | X-CLARITY™ Mounting Solution Value Pack | 10 x 25 mL |
| C13107 | X-CLARITY™ Mounting Solution Bulk Pack | 20 x 25 mL |
