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LABELING & IMAGING
DeepLabel™ Antibody Staining Kit for Cleared Tissues
DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging.
ANTIBODY LABELING FOR CLEARED TISSUES
ANTIBODY LABELING
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DeepLabel™ Antibody Staining Kit C33001DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues (compatible with CLARITY, PACT, iDISCO, CUBIC, etc).SFN 2018 POSTER
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Compatible with
multiple clearing methods -
Simple protocol
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Vibrant imaging at
subcellular resolution -
Site-specific labeling
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Homogenous antibody distribution
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2.6 X greater signal-to-background
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Without DeepLabel
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With DeepLabel
Adult mouse brain tissue stained with anti-Collagen IV (red) and imaged with a confocal microscope.
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Adult mouse brain stained using DeepLabel with anti-β-Amyloid (yellow), anti-Iba1 (red), and anti-SMA (purple).
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Whole adult Drosophila brain stained using DeepLabel with anti-GFP (green) and anti-TH (red).
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Whole adult mouse brain stained using DeepLabel with anti-TH (red).
Cat # | Product | Qty | MSDS | Product Insert | ||||||
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C33001 | DeepLabel™ Antibody Staining Kit | 1 kit |
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C33002 | DeepLabel™ Solution A | 25 mL |
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C33003 | DeepLabel™ Solution B | 25 mL |
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IMAGING
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X-CLARITY™ Mounting Solution C13101 | C13102 | C13107X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS) modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.App Note
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RI = 1.46 at 25°C
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Minimizes photobleaching
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Preserves fluorescence
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Before ETC
PFA-fixed (1 week) -
After ETC
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After RI matching
Seoul National University College of Medicine
Okinawa Institute of Science and Technology
Cat # | Product | Qty | MSDS | Product Insert | ||||||
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C13101 | X-CLARITY™ Mounting Solution | 1 x 25 mL |
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WHERE THE DEEPLABEL™ HAS BEEN CITED
Citations
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Multiplex immunolabeling and imaging of functionally essential kidney structures in X-CLARITY-cleared tissue.
2019. Mochama P, Tyshynsky R, Sanders MA. Microscopy and Microanalysis 25(Supplement S2): 1260-1261.
Resources
Downloads
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Logos Biosystems Products
Brochure
2020-04-26 | 2.14 MB -
The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY
Poster
2018-11-26 | 1.25 MB -
DeepLabel™ Antibody Staining Kit Flyer VDL1810-01
Brochure
2018-11-02 | 458.10 KB -
X-CLARITY™ Systems and Reagents for Tissue Clearing Brochure VX1901-01
Brochure
2018-05-18 | 962KB -
The X-CLARITY™ Mounting Solution: an improved RI matching solution for tissues cleared by the X-CLARITY™ Tissue Clearing System
Product literature
2018-04-17 | 1.5 MB
ORDERING INFORMATION
Cat # | Product | Qty |
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C33001 | DeepLabel™ Antibody Staining Kit - DeepLabel™ Solution A (1 x 25 mL) - DeepLabel™ Solution B (2 x 25 mL) - DeepLabel™ Washing Buffer (1 x 250 mL) |
1 kit |
C33002 | DeepLabel™ Solution A | 1 x 25 mL |
C33003 | DeepLabel™ Solution B | 2 x 25 mL |
C33004 | DeepLabel™ Washing Buffer | 1 x 250 mL |
C13101 | X-CLARITY™ Mounting Solution | 1 x 25 mL |
C13102 | X-CLARITY™ Mounting Solution Value Pack | 10 x 25 mL |
C13107 | X-CLARITY™ Mounting Solution Bulk Pack | 20 x 25 mL |