• DeepLabel Antibody Labeling Kit for Cleared Tissues
  • LABELING & IMAGING

    DeepLabel™ Antibody Staining Kit for Cleared Tissues

    DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging.

ANTIBODY LABELING FOR CLEARED TISSUES

Clearing methods allow light to penetrate samples for imaging, although the method used can affect tissue permeability to molecular probes. Conventional labeling protocols involve the slow diffusion of probes into thin sample sections, which translates to a time-consuming impracticality when applied to thicker samples. The slow progression of diffusion can also lead to uneven staining, with higher, nonspecific binding on the outside of a thicker sample. The new DeepLabel™ Antibody Staining Kit from Logos Biosystems overcomes these obstacles. Optimized for thick, cleared tissues, DeepLabel™ increases tissue permeability and enhances antigen-antibody binding so that antibody labeling of cleared tissues can happen rapidly and efficiently.

ANTIBODY LABELING

  • DeepLabel™ Antibody Staining Kit C33001
    DeepLabel™ Antibody Staining Kit is a set of non-toxic, ready-to-use reagents optimized for use with clarified tissues for effective antibody penetration and site-specific binding. With DeepLabel™, macromolecular probes rapidly and efficiently penetrate thick, protein-dense tissues at lower antibody concentrations. DeepLabel™ facilitates homogenous antibody staining with 2.6 X greater signal-to-background than conventional staining methods. DeepLabel™ is compatible with virtually all antibodies and all cleared tissues (compatible with CLARITY, PACT, iDISCO, CUBIC, etc).
    SFN 2018 POSTER
  • Antibody labeling DeepLabel Antibody Staining Kit
  • Compatible with
    multiple clearing methods
  • Simple protocol
  • Vibrant imaging at
    subcellular resolution
  • Site-specific labeling
  • Homogenous antibody distribution
  • 2.6 X greater signal-to-background
The effect of DeepLabel™ on anti-Collagen IV penetration into mouse brain tissues
  • Without DeepLabel
    LogosBiosystems Without DeepLabel
  • With DeepLabel
    LogosBiosystems DeepLabel on anti-Collagen IV penetration into mouse brain tissues

Adult mouse brain tissue stained with anti-Collagen IV (red) and imaged with a confocal microscope.

  • DeepLabel Adult mouse brain stained using DeepLabel with anti-β-Amyloid, anti-Iba1, and anti-SMA

    Adult mouse brain stained using DeepLabel with anti-β-Amyloid (yellow), anti-Iba1 (red), and anti-SMA (purple).

  • Whole adult Drosophila brain stained using DeepLabel with anti-GFP (green) and anti-TH (red)

    Whole adult Drosophila brain stained using DeepLabel with anti-GFP (green) and anti-TH (red).

  • Whole adult mouse brain stained using DeepLabel with anti-TH (red)

    Whole adult mouse brain stained using DeepLabel with anti-TH (red).

DEEPLABEL
    Cat # Product Qty MSDS Product Insert
    C33001 DeepLabel™ Antibody Staining Kit

    1 kit

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    C33001 DeepLabel™ Antibody Staining Kit MSDS.zipDownload 
    C33001 DeepLabel™ Antibody Staining Kit VDL1901-02.pdfDownload 
    C33002 DeepLabel™ Solution A

    25 mL

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    C33001 DeepLabel™ Antibody Staining Kit VDL1901-02.pdfDownload 
    C33003 DeepLabel™ Solution B

    25 mL

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    C33003 DeepLabel™ Solution B MSDS.pdfDownload 
    C33001 DeepLabel™ Antibody Staining Kit VDL1901-02.pdfDownload 
    C33004 DeepLabel™ Washing Buffer

    250 mL

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    C33001 DeepLabel™ Antibody Staining Kit VDL1901-02.pdfDownload 
    C13101 X-CLARITY™ Mounting Solution

    1 x 25 mL

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    C13101 X-CLARITY™ Mounting Solution MSDS.pdfDownload 
    C13101,2,7 X-CLARITY™ Mounting Solution VX1802-01.pdfDownload 
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    IMAGING

    • X-CLARITY™ Mounting Solution C13101  |  C13102  |  C13107
      X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS) modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.
      App Note
    • Imaging X-CLARITY Mounting Solution
    • RI = 1.46 at 25°C
    • Minimizes photobleaching
    • Preserves fluorescence
    Human cerebral cortex (1 mm)
    • X-CLARITY Mounting Solution Human cerebral cortex (1 mm) Before ETC PFA-fixed Seoul National University College of Medicine Hyung Jin Choi
      Before ETC
      PFA-fixed (1 week)
    • X-CLARITY Mounting Solution Human cerebral cortex (1 mm) After ETCSeoul National University College of Medicine Hyung Jin Choi
      After ETC
    • LogosBiosystems X-CLARITY Mounting Solution Human cerebral cortex (1 mm) After RI matching Seoul National University College of Medicine Hyung Jin Choi
      After RI matching
    Courtesy of Hyung Jin Choi, PhD
    Seoul National University College of Medicine
    Volumetric images of auditory neurons in a zebra finch brain (3 mm)
    • Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution - PI & GFP
    • Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution - GFP 500 um
    • Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution - GFP 50 um
    Volumetric images of auditory neurons in zebra finch brain slices cleared with the X-CLARITY and RI-matched with X-CLARITY Mounting Solution. Auditory neurons of a zebra finch were labeled with GFP bilaterally via injection of a viral vector encoding GFP, stained with propidium iodide (PI) to visualize sample structure, and imaged with a light sheet microscope.
    Courtesy of Makoto Araki, PhD and Yuichi Morohashi, PhD
    Okinawa Institute of Science and Technology
    MOUNTING SOLUTION
      Cat # Product Qty MSDS Product Insert
      C13101 X-CLARITY™ Mounting Solution

      1 x 25 mL

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      C13101 X-CLARITY™ Mounting Solution MSDS.pdfDownload 
      C13101,2,7 X-CLARITY™ Mounting Solution VX1802-01.pdfDownload 
      C13102 X-CLARITY™ Mounting Solution Value Pack

      10 x 25 mL

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      C13101 X-CLARITY™ Mounting Solution MSDS.pdfDownload 
      C13101,2,7 X-CLARITY™ Mounting Solution VX1802-01.pdfDownload 
      C13107 X-CLARITY™ Mounting Solution Bulk Pack

      20 x 25 mL

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      C13101,2,7 X-CLARITY™ Mounting Solution VX1802-01.pdfDownload 
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      WHAT DO OUR CUSTOMERS HAVE TO SAY?

      Testimonials
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      WHERE THE DEEPLABEL™ HAS BEEN CITED

      Citations
      • Multiplex immunolabeling and imaging of functionally essential kidney structures in X-CLARITY-cleared tissue.
        2019. Mochama P, Tyshynsky R, Sanders MA. Microscopy and Microanalysis 25(Supplement S2): 1260-1261.

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      Resources

      Downloads
      • Icon
        Logos Biosystems Products

        Brochure

        2019-07-16 | 2.14 MB
      • Icon
        The effects of DeepLabel Antibody Staining Kit on antibody penetration in diverse organs after tissue clearing with the X-CLARITY

        Poster

        2018-11-26 | 1.25 MB
      • Icon
        DeepLabel™ Antibody Staining Kit Flyer VDL1810-01

        Brochure

        2018-11-02 | 458.10 KB
      • Icon
        X-CLARITY™ Systems and Reagents for Tissue Clearing Brochure VX1901-01

        Brochure

        2018-05-18 | 962KB
      • Icon
        The X-CLARITY™ Mounting Solution: an improved RI matching solution for tissues cleared by the X-CLARITY™ Tissue Clearing System

        Product literature

        2018-04-17 | 1.5 MB

          ORDERING INFORMATION

          Cat # Product Qty
          C33001 DeepLabel™ Antibody Staining Kit
          - DeepLabel™ Solution A (1 x 25 mL)
          - DeepLabel™ Solution B (2 x 25 mL)
          - DeepLabel™ Washing Buffer (1 x 250 mL)
          1 kit
          C33002 DeepLabel™ Solution A 1 x 25 mL
          C33003 DeepLabel™ Solution B 2 x 25 mL
          C33004 DeepLabel™ Washing Buffer 1 x 250 mL
          C13101 X-CLARITY™ Mounting Solution 1 x 25 mL
          C13102 X-CLARITY™ Mounting Solution Value Pack 10 x 25 mL
          C13107 X-CLARITY™ Mounting Solution Bulk Pack 20 x 25 mL