Yeast Viability Kit 1

F23202

Yeast Viability Kit 1 is used to analyze the cell concentration and viability of yeast samples with the automated fluorescence cell counters of the LUNA™ family.

Fluorescein Diacetate Stain and Propidium Iodide Stain for Yeast are used to assess cell viability. Viable cells hydrolyze fluorescein diacetate into the green fluorescent compound fluorescein, causing viable cells to fluoresce green. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes binding to the nucleus of nonviable cells and causing them to fluoresce red.

Fluorescence Signal Enhancer 1 is an inhibitor of ATP-dependent fluorescein efflux. By blocking fluorescein from being pumped out of the cell, Fluorescence Signal Enhancer 1 lowers background and increases signal intensity, thereby enhancing the fluorescein signalto-noise ratio. Fluorescence Signal Enhancer 1 is most effective with metabolically active cells in the log growth phase.

Cell Dilution Buffer is for washing cells and diluting cell suspensions.



Fluorescein Diacetate Stain
Molecular formula C24H16O7
Molecular weight 416.38 g/mol
Appearance Clear liquid
Cell permeability Membrane permeant
Excitation/emission 494/521 nm (when converted to fluorescein)
Quantity 1 x 0.5 mL
Storage 4°C in the dark


Propidium Iodide Stain for Yeast
Molecular formula C27H34I2N4
Molecular weight 668.39 g/mol
Appearance Clear liquid
Cell permeability Membrane permeant
Excitation/emission 493/636 nm (in aqueous solution)
533/617 nm (when bound to nucleic acids)
Quantity 1 x 0.5 mL
Storage 4°C in the dark


Fluorescence Signal Enhancer 1
Appearance Clear liquid
Quantity 1 x 0.5 mL
Storage -20 °C in the dark


Cell Dilution Buffer
Appearance Clear liquid
Quantity 1 x 20 mL
Storage Room temperature


This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
Additional information is available on our website at www.logosbio.com