Acridine Orange Stain


Acridine Orange Stain is a cell-permeant vital dye that binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red.

Acridine Orange Stain can be used with Propidium Iodide Stain (F23003) to assess cell viability with the automated fluorescence cell counters of the LUNA™ family. Viable nucleated cells will fluoresce green and nonviable nucleated cells will fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results.

Product Description
Molecular formula C17H20ClN3
Molecular weight 301.82 g/mol
Appearance Clear liquid
Cell permeability Membrane permeant
Excitation/emission 500/526 nm (when bound to DNA)
460/650nm (when bound to ssDNA or RNA)
Quantity 2 x 0.5 mL
Storage 4 °C in the dark

This product is for research use only. Please consult the material safety data sheet for information regarding hazards and safe handling practices.
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