Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells.
2021. Shim MS, Nettesheim A, Dixon A, Liton PB. Proceedings of the National Academy of Sciences of the United States of America 118(13):e2021942118.
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Cell Imaging
CELENA® X High Content Imaging System
Automated high content image acquisition and analysis for drug discovery and cell biologydownload software
The CELENA® X High Content Imaging System is an integrated imaging system designed for rapid, high content image acquisition and analysis. Customizable imaging protocols, image-based and laser autofocusing modules, and a motorized XYZ stage simplify well plate imaging and slide scanning. The integrated CELENA® X Cell Analyzer software processes images and data for quantitative analysis. Analysis pipelines can be put together and reused to identify cellular or subcellular objects, process images for optimal data collection, and make various measurements.
The CELENA® X is as flexible as it is powerful, with interchangeable objectives and filter cubes to accommodate a wide range of fixed and live cell imaging applications.
SIMPLE HIGH CONTENT IMAGING FOR QUANTITATIVE IMAGE-BASED ANALYSIS
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FULLY AUTOMATED PLATE AND SLIDE IMAGING · Automated vessel handling and scanning · Motorized XYZ stage, filter cube stage, and objective turret -
LASER AUTOFOCUS · Rapid and reproducible focusing · Minimized phototoxicity and photobleaching -
LIVE CELL ASSAY SUPPORT Onstage incubation system for a variety of experiments in physiological and non-physiological conditions -
FOUR IMAGING MODES Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging -
POWERFUL, EASY-TO-USE USER INTERFACE · Simple setup of imaging protocols · Seamless integration of imaging and data analysis processes -
CUSTOMIZABLE HIGH CONTENT ANALYSIS · Create and customize image analysis projects · Quantitatively analyze multiple image-based phenotypes
CREATE YOUR OWN IMAGING AND ANALYSIS WORKFLOWS WITH THE CELENA® X
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Imaging· Raw images · Metadata -
Image analysis· Morphology of individual cells and organelles · Spatial distribution of targets · Multiple measurements per cell · Differentiation of multiple phenotypes -
Data analysis· Analyzed images · Result files · Graphs (coming soon)
ABUNDANT APPLICATIONS
The CELENA ® X Cell Analyzer software is incredibly powerful and its ability to batch process makes it ideal for high content analysis. The powerful and flexible software allows for the creation and customization of workflows that can be used for the simplest fixed cell assays to more complicated, time-lapse live cell assays.-
Apoptosis
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Autophagy
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Cell cycle
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Cell growth/proliferation
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Cell morphology
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Cell viability/toxicity
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Co-localization
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Confluency
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Histology
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Immunofluorescence
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NF-κB translocation
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Organoid/spheroid morphology
VERSATILE AND CUSTOMIZABLE FOR YOUR CELL IMAGING NEEDS
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Objective Selection Guide
Download our objective selection guide to see the Olympus objectives we recommend for the CELENA® X.
Objective Selection Guide -
LED Filter Cube Selection Guide
Download our LED filter cube selection guide to find which filter cubes fit your needs the best.
LED Filter Cube Selection Guide
WHAT DO OUR CUSTOMERS HAVE TO SAY?
Testimonials
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David Benz
Institut für Zellbiologie und Immunologie Stuttgart
Perfect combination of high content and high quality imaging.
The speed of image acquisition is outstanding and the images generated are of high quality. Together with the easy-to-use software, it is a great product. -
Myoung Sup Shim, PhD
Duke University Health System
The best automatic digital imaging system
We need live cell time-lapse imaging for our studies but other imaging systems did not produce acceptable images for long-term imaging. The CELENA X is the only system that has given us satisfactory multi-fluorescence images of multi-well plates. The automatic laser focusing is especially fantastic. We don’t waste time taking individual images as the CELENA X does high-throughput imaging and focuses great for all wells over long periods of time. Image quality is great for every photo and making high-quality videos is easy. Cells remain healthy, and there is minimal fluorescence quenching even after extensive imaging (5 minute intervals for over 72 hours). I strongly recommend the CELENA X for live cell time-lapse imaging and other applications.
WHERE THE celena® x HAS BEEN CITED
Citations
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Cathepsin B Localizes in the Caveolae and Participates in the Proteolytic Cascade in Trabecular Meshwork Cells. Potential New Drug Target for the Treatment of Glaucoma.
2021. Nettesheim A, Shim MS, Dixon A, Raychaudhuri U, Gong H, Liton PB. Journal of Clinical Medicine 10(1):78. -
Indirubin-3'-monoxime induces paraptosis in MDA-MB-231 breast cancer cells by transmitting Ca 2+ from endoplasmic reticulum to mitochondria.
2021. Dilshara MG, Neelaka Molagoda IM, Prasad Tharanga Jayasooriya RG, Choi YH, Park C, Kim GY. Archives of Biochemistry and Biophysics 698:108723. -
Revascularization and limb salvage following critical limb ischemia by nanoceria-induced Ref-1/APE1-dependent angiogenesis.
2020. Park IS, Mahapatra C, Park JS, Dashnyam K, Kim JW, Ahn JC, Chung PS, Yoon DS, Mandakhbayar N, Singh RK, Lee JH, Leong KW, Kim HW. Biomaterials 242:119919. -
Synthesis and Properties of CurNQ for the Theranostic Application in Ovarian Cancer Intervention.
2020. Freidus LG, Kumar P, Marimuthu T, Pradeep P, Pillay V, Choonara YE. Molecules (Basel, Switzerland) 25(19):4471. -
The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.
2019. Shim MS, Nettesheim A, Hirt J, Liton PB. Autophagy 16: 1-14.
Resources
Downloads
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CELENA®X High Content Imaging SystemInstallation Guide
2021-06-18 | 665.56 KB -
CELENA® XBrochure
2019-11-13 | 2 MB -
Transfection_DAPI,GFP_10X.cxpipePipeline
2019-08-07 | 3.49 KB -
Cell counting_DAPI,GFP_10X.cxpipePipeline
2019-07-17 | 2.78 KB -
Confluency_BF_4X.cxpipePipeline
2019-07-10 | 9.23 KB -
A multiparameter assessment of the dose-dependent cytotoxicity of Camptothecin on HeLa cellsProduct literature
2019-05-28 | 456.95 KB -
Evaluating transfection efficiency using the CELENA X
Product literature
2019-03-25 | 422.40 KB -
Counting adherent cells in multi-well plates with the CELENA X
Product literature
2019-03-07 | 356.63 KB -
Wound healing analysis based on image segmentation
Product literature
2019-01-16 | 574.17 KB -
Non-destructive quantification of cytotoxicity in live HeLa cells using the CELENA® X
Product literature
2018-12-07 | 417.63 KB
Videos
CELENA X Product Demo: How-to-use the high contents imaging and analysis system
2020-03-20Product Introduction: CELENA X High contents imaging & analysis system
2020-03-20Studying dynamic changes in glial cell morphology with the CELENA X High Content Imaging System
2019-12-13Calculating GFP transfection efficiency
2019-08-06Identifying and counting fluorescence-stained adherent cells
2019-07-16Confluency analysis from brightfield images
2019-07-09ORDERING INFORMATION
| Cat # | Product | Qty |
|---|---|---|
| CX30000 | CELENA® X High Content Imaging System | 1 unit |
| I10530 | CX Stage Top Incubator, Basic [Tokai] | 1 set |
| I10531 | CX Stage Top Incubator, Pro [Tokai] | 1 set |
| I10500 | Heating System for Multi-well Plates [Ibidi] | 1 set |
| I10502 | Gas Incubation System for CO2 [Ibidi] | 1 set |
| I10503 | Gas Incubation System for CO2/O2 [Ibidi] | 1 set |
| I10030 | UPLFLN 4X, Olympus | 1 unit |
| I10031 | UPLFLN 10X2, Olympus | 1 unit |
| I10034 | LUCPLFLN 20X, Olympus | 1 unit |
| I10035 | LUCPLFLN 40X, Olympus | 1 unit |
| I10038 | UPLFLN 4XPH, Olympus | 1 unit |
| I10039 | UPLFLN 10X2PH, Olympus | 1 unit |
| I10042 | LUCPLFLN 20XPH, Olympus | 1 unit |
| I10043 | LUCPLFLN 40XPH, Olympus | 1 unit |
| I10046 | PLAPON 1.25X, Olympus | 1 unit |
| I10047 | PLAPON 2X, Olympus | 1 unit |
| I10050 | UPLSAPO 60XO, Olympus | 1 unit |
| I10051 | UPLSAPO 100XO, Olympus | 1 unit |
| I10005 | TC PlanFluor 4X | 1 unit |
| I10006 | TC PlanFluor 10X | 1 unit |
| I10007 | TC PlanFluor 20X | 1 unit |
| I10008 | TC PlanFluor 40X | 1 unit |
| I10013 | Plan Apochromat Fluor 1.25X | 1 unit |
| I10014 | Plan Apochromat Fluor 4X | 1 unit |
| I10009 | Plan Apochromat Fluor 10X | 1 unit |
| I10010 | Plan Apochromat Fluor 20X | 1 unit |
| I10011 | Plan Apochromat Fluor 40X | 1 unit |
| I10015 | Plan Apochromat Fluor Oil 40X | 1 unit |
| I10012 | Plan Apochromat Fluor Oil 100X | 1 unit |
| I10130 | DAPI | 1 unit |
| I10131 | EGFP | 1 unit |
| I10132 | RFP | 1 unit |
| I10133 | mCherry | 1 unit |
| I10134 | ECFP | 1 unit |
| I10135 | EYFP | 1 unit |
| I10136 | DSRed | 1 unit |
| I10137 | Cy5 | 1 unit |
| I10138 | Cy7 | 1 unit |
| I10139 | Cy3/TRITC Long Pass | 1 unit |
| I10140 | GFP Long Pass | 1 unit |
| I10141 | Cy5 Long Pass | 1 unit |
| I10142 | Custom Filters | 1 unit |
| I10410 | Joystick | 1 unit |