The CELENA® X High Content Imaging System is an integrated imaging system designed for rapid, high content image acquisition and analysis. Customizable imaging protocols, image-based and laser autofocusing modules, and a motorized XYZ stage simplify well plate imaging and slide scanning. The integrated CELENA® X Cell Analyzer software processes images and data for quantitative analysis. Analysis pipelines can be put together and reused to identify cellular or subcellular objects, process images for optimal data collection, and make various measurements. The CELENA® X is as flexible as it is powerful, with interchangeable objectives and filter cubes to accommodate a wide range of fixed and live cell imaging applications.

SIMPLE HIGH CONTENT IMAGING FOR QUANTITATIVE IMAGE-BASED ANALYSIS

  • FULLY AUTOMATED PLATE AND SLIDE IMAGING
    FULLY AUTOMATED PLATE AND SLIDE IMAGING · Automated vessel handling and scanning · Motorized XYZ stage, filter cube stage, and objective turret
  • LASER AUTOFOCUS
    LASER AUTOFOCUS · Rapid and reproducible focusing · Minimized phototoxicity and photobleaching
  • LIVE CELL ASSAY SUPPORT
    LIVE CELL ASSAY SUPPORT Onstage incubation system for a variety of experiments in physiological and non-physiological conditions
  • FOUR IMAGING MODES
    FOUR IMAGING MODES Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging
  • POWERFUL, EASY-TO-USE USER INTERFACE
    POWERFUL, EASY-TO-USE USER INTERFACE · Simple setup of imaging protocols · Seamless integration of imaging and data analysis processes
  • CUSTOMIZABLE HIGH CONTENT ANALYSIS
    CUSTOMIZABLE HIGH CONTENT ANALYSIS · Create and customize image analysis projects · Quantitatively analyze multiple image-based phenotypes

CREATE YOUR OWN IMAGING AND ANALYSIS WORKFLOWS WITH THE CELENA® X

  • Imaging
    · Raw images · Metadata
  • Image analysis
    · Morphology of individual cells and organelles · Spatial distribution of targets · Multiple measurements per cell · Differentiation of multiple phenotypes
  • Data analysis
    · Analyzed images · Result files · Graphs (coming soon)

ABUNDANT APPLICATIONS

The CELENA ® X Cell Analyzer software is incredibly powerful and its ability to batch process makes it ideal for high content analysis. The powerful and flexible software allows for the creation and customization of workflows that can be used for the simplest fixed cell assays to more complicated, time-lapse live cell assays.
  • Apoptosis
  • Autophagy
  • Cell cycle
  • Cell growth/proliferation
  • Cell morphology
  • Cell viability/toxicity
  • Co-localization
  • Confluency
  • Histology
  • Immunofluorescence
  • NF-κB translocation
  • Organoid/spheroid morphology
Evaluating transfection efficiency using the CELENA X Counting adherent cells in multi-well plates with the CELENA X Wound healing analysis based on image segmentation Non-destructive quantification of cytotoxicity in live HeLa cells using the CELENA® X CELENA®X Quick Start Guide CELENA® X Brochure

VERSATILE AND CUSTOMIZABLE FOR YOUR CELL IMAGING NEEDS

  • Icon Objective Selection Guide

    Download our objective selection guide to see the Olympus objectives we recommend for the CELENA® X.

    Objective Selection Guide
  • Icon LED Filter Cube Selection Guide

    Download our LED filter cube selection guide to find which filter cubes fit your needs the best.

    LED Filter Cube Selection Guide

    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Peter Davis

      ATA Scientific

      Great to use, inexpensive - watch out big microscopy companies... 
      The Celena-X is so easy to use, it has huge flexibility and it is oh so affordable making the 'big name brands' look like a rip off!

    • David Benz

      Institut für Zellbiologie und Immunologie Stuttgart

      Perfect combination of high content and high quality imaging.
      The speed of image acquisition is outstanding and the images generated are of high quality. Together with the easy-to-use software, it is a great product.

    • Myoung Sup Shim, PhD

      Duke University Health System

      The best automatic digital imaging system
      We need live cell time-lapse imaging for our studies but other imaging systems did not produce acceptable images for long-term imaging. The CELENA X is the only system that has given us satisfactory multi-fluorescence images of multi-well plates. The automatic laser focusing is especially fantastic. We don’t waste time taking individual images as the CELENA X does high-throughput imaging and focuses great for all wells over long periods of time. Image quality is great for every photo and making high-quality videos is easy. Cells remain healthy, and there is minimal fluorescence quenching even after extensive imaging (5 minute intervals for over 72 hours). I strongly recommend the CELENA X for live cell time-lapse imaging and other applications.

    show more  + + SHOW OUR CUSTOMERS

    WHERE THE celena® x HAS BEEN CITED

    Citations
    • Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells.
      2021. Shim MS, Nettesheim A, Dixon A, Liton PB. Proceedings of the National Academy of Sciences of the United States of America 118(13):e2021942118.

    • Cathepsin B Localizes in the Caveolae and Participates in the Proteolytic Cascade in Trabecular Meshwork Cells. Potential New Drug Target for the Treatment of Glaucoma.
      2021. Nettesheim A, Shim MS, Dixon A, Raychaudhuri U, Gong H, Liton PB. Journal of Clinical Medicine 10(1):78.

    • Indirubin-3'-monoxime induces paraptosis in MDA-MB-231 breast cancer cells by transmitting Ca 2+ from endoplasmic reticulum to mitochondria.
      2021. Dilshara MG, Neelaka Molagoda IM, Prasad Tharanga Jayasooriya RG, Choi YH, Park C, Kim GY. Archives of Biochemistry and Biophysics 698:108723.

    • Revascularization and limb salvage following critical limb ischemia by nanoceria-induced Ref-1/APE1-dependent angiogenesis.
      2020. Park IS, Mahapatra C, Park JS, Dashnyam K, Kim JW, Ahn JC, Chung PS, Yoon DS, Mandakhbayar N, Singh RK, Lee JH, Leong KW, Kim HW. Biomaterials 242:119919.

    • Synthesis and Properties of CurNQ for the Theranostic Application in Ovarian Cancer Intervention.
      2020. Freidus LG, Kumar P, Marimuthu T, Pradeep P, Pillay V, Choonara YE. Molecules (Basel, Switzerland) 25(19):4471.

    • The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.
      2019. Shim MS, Nettesheim A, Hirt J, Liton PB. Autophagy 16: 1-14.

    SHOW MORE  +

    Resources

    Downloads
    • Icon
      CELENA®X vessel holder dimension

      Selection Guide

      2021-07-01 | 282.34 KB
    • Icon
      CELENA®X High Content Imaging System

      Installation Guide

      2021-06-18 | 665.56 KB
    • Icon
      CELENA® X

      Brochure

      2019-11-13 | 2 MB
    • Icon
      Transfection_DAPI,GFP_10X.cxpipe

      Pipeline

      2019-08-07 | 3.49 KB
    • Icon
      Cell counting_DAPI,GFP_10X.cxpipe

      Pipeline

      2019-07-17 | 2.78 KB
    • Icon
      Confluency_BF_4X.cxpipe

      Pipeline

      2019-07-10 | 9.23 KB
    • Icon
      CELENA®X

      Quick Start Guide

      2018-11-30 | 901.13 KB
      • Icon
        Fluorescence-based calcium mobilization assay with the CELENA® X

        Application note

        2021-09-07 | 455.78 KB
      • Icon
        A multiparameter assessment of the dose-dependent cytotoxicity of Camptothecin on HeLa cells

        Application note

        2019-05-28 | 456.95 KB
      • Icon
        Evaluating transfection efficiency using the CELENA X

        Application note

        2019-03-25 | 422.40 KB
      • Icon
        Counting adherent cells in multi-well plates with the CELENA X

        Application note

        2019-03-07 | 356.63 KB
      • Icon
        Wound healing analysis based on image segmentation

        Application note

        2019-01-16 | 574.17 KB
      • Icon
        Non-destructive quantification of cytotoxicity in live HeLa cells using the CELENA® X

        Application note

        2018-12-07 | 417.63 KB
        Videos

        CELENA X Product Demo: How-to-use the high contents imaging and analysis system

        Product Introduction: CELENA X High contents imaging & analysis system

        Studying dynamic changes in glial cell morphology with the CELENA X High Content Imaging System

        Calculating GFP transfection efficiency

        Identifying and counting fluorescence-stained adherent cells

        Confluency analysis from brightfield images

        ORDERING INFORMATION

        Cat #ProductQty
        CX30000CELENA® X High Content Imaging System1 unit
        I10530CX Stage Top Incubator, Basic [Tokai]1 set
        I10531CX Stage Top Incubator, Pro [Tokai]1 set
        I10500Heating System for Multi-well Plates [Ibidi]1 set
        I10502Gas Incubation System for CO2 [Ibidi]1 set
        I10503Gas Incubation System for CO2/O2 [Ibidi]1 set
        I10030UPLFLN 4X, Olympus1 unit
        I10031UPLFLN 10X2, Olympus1 unit
        I10034LUCPLFLN 20X, Olympus1 unit
        I10035LUCPLFLN 40X, Olympus1 unit
        I10038UPLFLN 4XPH, Olympus1 unit
        I10039UPLFLN 10X2PH, Olympus1 unit
        I10042LUCPLFLN 20XPH, Olympus1 unit
        I10043LUCPLFLN 40XPH, Olympus1 unit
        I10046PLAPON 1.25X, Olympus1 unit
        I10047PLAPON 2X, Olympus1 unit
        I10050UPLSAPO 60XO, Olympus1 unit
        I10051UPLSAPO 100XO, Olympus1 unit
        I10005TC PlanFluor 4X1 unit
        I10006TC PlanFluor 10X1 unit
        I10007TC PlanFluor 20X1 unit
        I10008TC PlanFluor 40X1 unit
        I10013Plan Apochromat Fluor 1.25X1 unit
        I10014Plan Apochromat Fluor 4X1 unit
        I10009Plan Apochromat Fluor 10X1 unit
        I10010Plan Apochromat Fluor 20X1 unit
        I10011Plan Apochromat Fluor 40X1 unit
        I10015Plan Apochromat Fluor Oil 40X1 unit
        I10012Plan Apochromat Fluor Oil 100X1 unit
        I10130DAPI1 unit
        I10131EGFP1 unit
        I10132RFP1 unit
        I10133mCherry1 unit
        I10134ECFP1 unit
        I10135EYFP1 unit
        I10136DSRed1 unit
        I10137Cy51 unit
        I10138Cy71 unit
        I10139Cy3/TRITC Long Pass1 unit
        I10140GFP Long Pass1 unit
        I10141Cy5 Long Pass1 unit
        I10142Custom Filters1 unit
        I10410Joystick1 unit