The CELENA® X High Content Imaging System is an integrated imaging system designed for rapid, high content image acquisition and analysis. Customizable imaging protocols, image-based and laser autofocusing modules, and a motorized XYZ stage simplify well plate imaging and slide scanning. The integrated CELENA® X Cell Analyzer software processes images and data for quantitative analysis. Analysis pipelines can be put together and reused to identify cellular or subcellular objects, process images for optimal data collection, and make various measurements. The CELENA® X is as flexible as it is powerful, with interchangeable objectives and filter cubes to accommodate a wide range of fixed and live cell imaging applications.

SIMPLE HIGH CONTENT IMAGING FOR QUANTITATIVE IMAGE-BASED ANALYSIS

  • FULLY AUTOMATED PLATE AND SLIDE IMAGING
    FULLY AUTOMATED PLATE AND SLIDE IMAGING · Automated vessel handling and scanning · Motorized XYZ stage, filter cube stage, and objective turret
  • LASER AUTOFOCUS
    LASER AUTOFOCUS · Rapid and reproducible focusing · Minimized phototoxicity and photobleaching
  • LIVE CELL ASSAY SUPPORT
    LIVE CELL ASSAY SUPPORT Onstage incubation system for a variety of experiments in physiological and non-physiological conditions
  • FOUR IMAGING MODES
    FOUR IMAGING MODES Fluorescence imaging in four channels, brightfield, color brightfield, and phase contrast imaging
  • POWERFUL, EASY-TO-USE USER INTERFACE
    POWERFUL, EASY-TO-USE USER INTERFACE · Simple setup of imaging protocols · Seamless integration of imaging and data analysis processes
  • CUSTOMIZABLE HIGH CONTENT ANALYSIS
    CUSTOMIZABLE HIGH CONTENT ANALYSIS · Create and customize image analysis projects · Quantitatively analyze multiple image-based phenotypes

CREATE YOUR OWN IMAGING AND ANALYSIS WORKFLOWS WITH THE CELENA® X

  • Imaging
    · Raw images · Metadata
  • Image analysis
    · Morphology of individual cells and organelles · Spatial distribution of targets · Multiple measurements per cell · Differentiation of multiple phenotypes
  • Data analysis
    · Analyzed images · Result files · Graphs (coming soon)

ABUNDANT APPLICATIONS

The CELENA ® X Cell Analyzer software is incredibly powerful and its ability to batch process makes it ideal for high content analysis. The powerful and flexible software allows for the creation and customization of workflows that can be used for the simplest fixed cell assays to more complicated, time-lapse live cell assays.
  • Apoptosis
  • Autophagy
  • Cell cycle
  • Cell growth/proliferation
  • Cell morphology
  • Cell viability/toxicity
  • Co-localization
  • Confluency
  • Histology
  • Immunofluorescence
  • NF-κB translocation
  • Organoid/spheroid morphology
Evaluating transfection efficiency using the CELENA X Counting adherent cells in multi-well plates with the CELENA X Wound healing analysis based on image segmentation Non-destructive quantification of cytotoxicity in live HeLa cells using the CELENA® X CELENA®X Quick Start Guide CELENA® X Brochure

VERSATILE AND CUSTOMIZABLE FOR YOUR CELL IMAGING NEEDS

  • Icon Objective Selection Guide

    Download our objective selection guide to see the Olympus objectives we recommend for the CELENA® X.

    Objective Selection Guide
  • Icon LED Filter Cube Selection Guide

    Download our LED filter cube selection guide to find which filter cubes fit your needs the best.

    LED Filter Cube Selection Guide

    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • David Benz

      Institut für Zellbiologie und Immunologie Stuttgart

      Perfect combination of high content and high quality imaging.
      The speed of image acquisition is outstanding and the images generated are of high quality. Together with the easy-to-use software, it is a great product.

    • Myoung Sup Shim, PhD

      Duke University Health System

      The best automatic digital imaging system
      We need live cell time-lapse imaging for our studies but other imaging systems did not produce acceptable images for long-term imaging. The CELENA X is the only system that has given us satisfactory multi-fluorescence images of multi-well plates. The automatic laser focusing is especially fantastic. We don’t waste time taking individual images as the CELENA X does high-throughput imaging and focuses great for all wells over long periods of time. Image quality is great for every photo and making high-quality videos is easy. Cells remain healthy, and there is minimal fluorescence quenching even after extensive imaging (5 minute intervals for over 72 hours). I strongly recommend the CELENA X for live cell time-lapse imaging and other applications.

    show more  + + SHOW OUR CUSTOMERS

    WHERE THE celena® x HAS BEEN CITED

    Citations
    • Primary cilia and the reciprocal activation of AKT and SMAD2/3 regulate stretch-induced autophagy in trabecular meshwork cells.
      2021. Shim MS, Nettesheim A, Dixon A, Liton PB. Proceedings of the National Academy of Sciences of the United States of America 118(13):e2021942118.

    • Cathepsin B Localizes in the Caveolae and Participates in the Proteolytic Cascade in Trabecular Meshwork Cells. Potential New Drug Target for the Treatment of Glaucoma.
      2021. Nettesheim A, Shim MS, Dixon A, Raychaudhuri U, Gong H, Liton PB. Journal of Clinical Medicine 10(1):78.

    • Indirubin-3'-monoxime induces paraptosis in MDA-MB-231 breast cancer cells by transmitting Ca 2+ from endoplasmic reticulum to mitochondria.
      2021. Dilshara MG, Neelaka Molagoda IM, Prasad Tharanga Jayasooriya RG, Choi YH, Park C, Kim GY. Archives of Biochemistry and Biophysics 698:108723.

    • Revascularization and limb salvage following critical limb ischemia by nanoceria-induced Ref-1/APE1-dependent angiogenesis.
      2020. Park IS, Mahapatra C, Park JS, Dashnyam K, Kim JW, Ahn JC, Chung PS, Yoon DS, Mandakhbayar N, Singh RK, Lee JH, Leong KW, Kim HW. Biomaterials 242:119919.

    • Synthesis and Properties of CurNQ for the Theranostic Application in Ovarian Cancer Intervention.
      2020. Freidus LG, Kumar P, Marimuthu T, Pradeep P, Pillay V, Choonara YE. Molecules (Basel, Switzerland) 25(19):4471.

    • The autophagic protein LC3 translocates to the nucleus and localizes in the nucleolus associated to NUFIP1 in response to cyclic mechanical stress.
      2019. Shim MS, Nettesheim A, Hirt J, Liton PB. Autophagy 16: 1-14.

    SHOW MORE  +

    Resources

    Downloads
    • Icon
      CELENA®X High Content Imaging System

      Installation Guide

      2021-06-18 | 665.56 KB
    • Icon
      CELENA® X

      Brochure

      2019-11-13 | 2 MB
    • Icon
      Transfection_DAPI,GFP_10X.cxpipe

      Pipeline

      2019-08-07 | 3.49 KB
    • Icon
      Cell counting_DAPI,GFP_10X.cxpipe

      Pipeline

      2019-07-17 | 2.78 KB
    • Icon
      Confluency_BF_4X.cxpipe

      Pipeline

      2019-07-10 | 9.23 KB
    • Icon
      A multiparameter assessment of the dose-dependent cytotoxicity of Camptothecin on HeLa cells

      Product literature

      2019-05-28 | 456.95 KB
    • Icon
      Evaluating transfection efficiency using the CELENA X

      Product literature

      2019-03-25 | 422.40 KB
    • Icon
      Counting adherent cells in multi-well plates with the CELENA X

      Product literature

      2019-03-07 | 356.63 KB
    • Icon
      Wound healing analysis based on image segmentation

      Product literature

      2019-01-16 | 574.17 KB
    • Icon
      Non-destructive quantification of cytotoxicity in live HeLa cells using the CELENA® X

      Product literature

      2018-12-07 | 417.63 KB
      Videos

      CELENA X Product Demo: How-to-use the high contents imaging and analysis system

      Product Introduction: CELENA X High contents imaging & analysis system

      Studying dynamic changes in glial cell morphology with the CELENA X High Content Imaging System

      Calculating GFP transfection efficiency

      Identifying and counting fluorescence-stained adherent cells

      Confluency analysis from brightfield images

      ORDERING INFORMATION

      Cat # Product Qty
      CX30000 CELENA® X High Content Imaging System 1 unit
      I10530 CX Stage Top Incubator, Basic [Tokai] 1 set
      I10531 CX Stage Top Incubator, Pro [Tokai] 1 set
      I10500 Heating System for Multi-well Plates [Ibidi] 1 set
      I10502 Gas Incubation System for CO2 [Ibidi] 1 set
      I10503 Gas Incubation System for CO2/O2 [Ibidi] 1 set
      I10030 UPLFLN 4X, Olympus 1 unit
      I10031 UPLFLN 10X2, Olympus 1 unit
      I10034 LUCPLFLN 20X, Olympus 1 unit
      I10035 LUCPLFLN 40X, Olympus 1 unit
      I10038 UPLFLN 4XPH, Olympus 1 unit
      I10039 UPLFLN 10X2PH, Olympus 1 unit
      I10042 LUCPLFLN 20XPH, Olympus 1 unit
      I10043 LUCPLFLN 40XPH, Olympus 1 unit
      I10046 PLAPON 1.25X, Olympus 1 unit
      I10047 PLAPON 2X, Olympus 1 unit
      I10050 UPLSAPO 60XO, Olympus 1 unit
      I10051 UPLSAPO 100XO, Olympus 1 unit
      I10005 TC PlanFluor 4X 1 unit
      I10006 TC PlanFluor 10X 1 unit
      I10007 TC PlanFluor 20X 1 unit
      I10008 TC PlanFluor 40X 1 unit
      I10013 Plan Apochromat Fluor 1.25X 1 unit
      I10014 Plan Apochromat Fluor 4X 1 unit
      I10009 Plan Apochromat Fluor 10X 1 unit
      I10010 Plan Apochromat Fluor 20X 1 unit
      I10011 Plan Apochromat Fluor 40X 1 unit
      I10015 Plan Apochromat Fluor Oil 40X 1 unit
      I10012 Plan Apochromat Fluor Oil 100X 1 unit
      I10130 DAPI 1 unit
      I10131 EGFP 1 unit
      I10132 RFP 1 unit
      I10133 mCherry 1 unit
      I10134 ECFP 1 unit
      I10135 EYFP 1 unit
      I10136 DSRed 1 unit
      I10137 Cy5 1 unit
      I10138 Cy7 1 unit
      I10139 Cy3/TRITC Long Pass 1 unit
      I10140 GFP Long Pass 1 unit
      I10141 Cy5 Long Pass 1 unit
      I10142 Custom Filters 1 unit
      I10410 Joystick 1 unit