• Microbial Cell Counter

    LUNA-II YF™ Automated Yeast Cell Counter

    Yeast strains used in food and drink production can be notoriously clumpy.
    The LUNA-II YF™ has an autofocusing cell counting and declustering algorithm that counts individual yeast cells in just 15 seconds.
    download software
As is well-known in brewing and winemaking industries, the quantity of yeast pitched to initiate fermentation directly affects the flavor, aroma, and quality of the final product. Although yeast can be pitched by weight or volume, such methods are inexact and highly variable. The ASBC method using methylene blue method is more accurate but time consuming and subjective. Use the LUNA-II YF™ to get accurate and reproducible yeast cell counts in as little as 15 seconds. This little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

WHAT THE LUNA-II YF™ DOES BEST

  • LUNA-II YF™ Automated Yeast Cell Counter Accurate yeast cell counts
    ACCURATE YEAST CELL COUNTS

    The LUNA-II YF™ provides cell count and viability data with the precision
    and consistency of a flow cytometer.

  • LUNA-II YF™ Automated Yeast Cell Counter 15 seconds to results
    15 SECONDS TO RESULTS

    Using dual fluorescence optics, an autofocusing liquid lens, and advance
    software, the LUNA-II YF™ produces data in just 15 seconds.

  • LUNA-II YF™ Automated Yeast Cell Counter Yeast-optimized analysis
    YEAST-OPTIMIZED ANALYSIS

    Unlike flow cytometers, the LUNA-II YF™ has advanced declustering
    capabilities, and can count even the clumpiest cells.

  • VERIFIED WITH MULTIPLE STRAINS

    We’ve independently tested 30+ brewing yeast products on the LUNA-II YF™.
    Visit the cell DB for a partial list.

THE SOFTWARE THAT CHANGES EVERYTHING.

AUTOFOCUSED COUNTING USE THE DILUTION CALCULATOR EXPORT IMAGES & DATA REPORTS CUSTOMIZE & SAVE PROTOCOLS

 

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Logos Biosystems LUNA Reusable Slide
LUNA-II YF™ flyer Brochure Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images Product literature A comparison of yeast cell counting methods: LUNA-II YF™ Automated Yeast Cell Counter vs. flow cytometry vs. hemocytometer Product literature Logos Biosystems Products Brochure LUNA-II YF™ software 1.0.1 Software
Compatible Reagents
    Cat # Product Qty MSDS Product Insert
    F23001 Acridine Orange/Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23001] Acridine Orange Propidium Iodide (AOPI) Stain.pdfDownload 
    F23001 Acridine Orange,Propidium Iodide Stain VL1801-01.pdfDownload 
    F23002 Acridine Orange Stain

    2 x 0.5 mL

    FileAction
    [F23002] Acridine Orange (AO) Stain.pdfDownload 
    F23002 Acridine Orange Stain VL1801-01.pdfDownload 
    F23003 Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23003] Propidium Iodide (PI) Stain.pdfDownload 
    F23003 Propidium Iodide Stain VL1801-01.pdfDownload 
    F23202 Yeast Viability Kit 1

    1 kit

    FileAction
    [F23202] Yeast Viability Kit 1.zipDownload 
    F23202 Yeast Viability Kit 1 VL1801-01.pdfDownload 
    Request CoA   >

    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    + SHOW OUR CUSTOMERS

    WHERE THE LUNA-II-YF™ HAS BEEN CITED

    Citations
    SHOW MORE  +

    Resources

    Downloads
    • Icon
      LUNA-II YF™ flyer VL1605-01

      Brochure

      2018-04-16 | 501 KB
    • Icon
      Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images

      Product literature

      2018-04-16 | 1.7 MB
    • Icon
      A comparison of yeast cell counting methods: LUNA-II YF™ Automated Yeast Cell Counter vs. flow cytometry vs. hemocytometer

      Product literature

      2018-04-16 | 492 KB
    • Icon
      Logos Biosystems Products

      Brochure

      2018-04-15 | 2.14 MB
    • Icon
      How to Count Cells An Overview of Cell Counting Methods

      Product literature

      2018-04-13 | 720.79 KB
      • Icon
        LUNA-II YF™ software 1.0.1

        Software

        2018-04-16 | 8.6 MB
        Videos

        How to Use the LUNA™ Reusable Slide

        Cell DB
          • LUNA-II YF Automated Yeast Cell Counter Fermentis Safberw WB-06 Wheat 01
          • Fermentis

            Fermentis Safbrew WB-06 Wheat Safbrew WB-06 Wheat yeast cells were stained with fluorescein diacetate and propidium iodide.

            Default Protocol: FDA-PI

            l Dilution factor: 1.18
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 4
            l Red fluorescence exposure: 6

          • Fermentis

            Fermentis Safbrew WB-06 Wheat Safbrew WB-06 Wheat yeast cells were stained with fluorescein diacetate and propidium iodide.

            Default Protocol: FDA-PI

            l Dilution factor: 1.18
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 4
            l Red fluorescence exposure: 6

          • Lallemand

            Munich Wheat Beer yeast cells were stained with acridine orange and propidium iodide.

            Default Protocol: AO-PI

            l Dilution factor: 1.11
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 7
            l Red fluorescence exposure: 3

          • LUNA-II YF Automated Yeast Cell Counter Nottingham Ale Beer yeast cells Lallemandd
          • Lallemand

            Nottingham Ale Beer yeast cells were stained with acridine orange and propidium iodide.

            Default Protocol: AO-PI

            l Dilution factor: 1.11
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 7
            l Red fluorescence exposure: 3

          • LUNA-II YF Automated Yeast Cell Counter Mangrove Jack’s Craft Series M15 Empire Ale yeast
          • Mangrove Jack’s

            Craft Series M15 Empire Ale yeast cells were stained with fluorescein diacetate and propidium iodide.

            Default Protocol: FDA-PI

            l Dilution factor: 1.18
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 4
            l Red fluorescence exposure: 6

          • LUNA-II YF Automated Yeast Cell Counter Mangrove Jack’s Craft Series M21 Belgian Wit yeast
          • Mangrove Jack’s

            Craft Series M21 Belgian Wit yeast cells were stained with acridine orange and propidium iodide.

            Default Protocol: AO-PI

            l Dilution factor: 1.11
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 7
            l Red fluorescence exposure: 3

          • LUNA-II YF Automated Yeast Cell Counter Mangrove-Jack’s Craft Series M29 French Saison Ale yeast
          • Mangrove Jack’s

            Craft Series M29 French Saison Ale yeast cells were stained with acridine orange and propidium iodide.

            Default Protocol: AO-PI

            l Dilution factor: 1.11
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 7
            l Red fluorescence exposure: 3

          • Mangrove Jack’s

            Craft Series M31 Belgian Tripel yeast cells were stained with fluorescein diacetate and propidium iodide.

            Default Protocol: FDA-PI

            l Dilution factor: 1.18
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 4
            l Red fluorescence exposure: 6

          • LUNA-II YF Automated Yeast Cell Counter Mangrove-Jack’s Craft Series M41 Belgian Ale yeast
          • Mangrove Jack’s

            Craft Series M41 Belgian Ale yeast cells were stained with acridine orange and propidium iodide.

            Default Protocol: AO-PI

            l Dilution factor: 1.11
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 7
            l Red fluorescence exposure: 3

          • Mangrove Jack’s

            Craft Series M44 US West Coast yeast cells were stained with fluorescein diacetate and propidium iodide.

            Default Protocol: FDA-PI

            l Dilution factor: 1.18
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 4
            l Red fluorescence exposure: 6

          • LUNA-II YF Automated Yeast Cell Counter Wyeast Canadian Belgian Ale yeast
          • Wyeast

            Canadian/Belgian Ale yeast cells were stained with acridine orange and propidium iodide.

            Default Protocol: AO-PI

            l Dilution factor: 1.11
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 7
            l Red fluorescence exposure: 3

          • LUNA-II YF Automated Yeast Cell Counter Wyeast Forbidden Fruit yeast
          • Wyeast

            Forbidden Fruit yeast cells were stained with fluorescein diacetate and propidium iodide.

            Default Protocol: FDA-PI

            l Dilution factor: 1.18
            l Min. fluorescent object size: 1 µm
            l Max. fluorescent object size: 60 µm
            l Green fluorescence exposure: 4
            l Red fluorescence exposure: 6

        show more  +

        ORDERING INFORMATION

        Cat # Product Qty
        L50001 LUNA-II YF™ Automated Yeast Cell Counter 1 unit
        L12005 PhotonSlide™, 50 Slides 1 box
        L12006 PhotonSlide™, 500 Slides 10 boxes
        L12007 PhotonSlide™, 1000 Slides 20 boxes
        F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
        F23002 Acridine Orange Stain 2 x 0.5 mL
        F23003 Propidium Iodide Stain 2 x 0.5 mL
        F53002 Cell Dilution Buffer II 5 x 20 mL
        F23202 Yeast Viability Kit 1
        - Propidium Iodide Stain for Yeast (1 x 0.5 mL)
        - Fluorescein Diacetate Stain (1 x 0.5 mL)
        - Cell Dilution Buffer (1 x 20 mL)
        - Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
        1 kit
        F23102 LUNA™ Fluorescence Calibration Beads 2 x 1 mL
        P10001 LUNA™ Printer 1 unit
        P12001 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls