• Fluorescence Cell Counter

    LUNA-FX7™ Automated Cell Counter

    All new optics and upgraded software algorithm with high-speed and precision autofocus.
    Options for a diverse range of slides (8-, 3-, 2-, 1-chamber formats).
    Data transfer via Wi-Fi, USB device, or Ethernet.
    CountWire™ software compliant with 21 CFR Part 11/GMP requirements.
    New bioprocess monitoring feature and validation slides for QC.
    download software
Introducing the LUNA-FX7™ - the newest member of the award-winning LUNA™ Cell Counter family. The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, new 1, 3 and 8-chamber slide options, state of the art optics, dual fluorescent and brightfield detection, precision autofocus, and 21 CFR Part 11 compliance. The LUNA-FX7™ has built-in quality control software and new validation slides for monitoring QC and production. By combining the trademark ease of use characteristic of all LUNA™ counters with the enhanced accuracy, higher throughput, and software upgrades - the LUNA-FX7™ will improve the efficiency and security of your lab's workflow.

WHAT THE LUNA-FX7™ DOES BEST

  • BEST IN CLASS ACCURACY
    BEST IN CLASS ACCURACY Improved counting algorithm and increased measuring volumes lower the CV, even less than 1%!
  • MULTIPLE SAMPLES IN ONE GO
    MULTIPLE SAMPLES IN ONE GO New 8- and 3-chamber slide increase the sample throughput.
  • EXPANDED CELL CONCENTRATION RANGE
    EXPANDED CELL CONCENTRATION RANGE (1.0E04 – 5.0E07 CELLS/ML) Suitable for diverse cell counting applications including single cell sequencing and CAR-T dosing determinations.
  • EXCELLENCE OF COUNTING BOTH BRIGHTFIELD AND FLUORESCENCE
    EXCELLENCE OF COUNTING BOTH BRIGHTFIELD AND FLUORESCENCE The fast and precise autofocus with an optimized software algorithm fulfils desired counting results.
  • CONFIDENCE, TRUST, & RELIABILITY
    CONFIDENCE, TRUST AND RELIABILITY Built-in QC software and validation slides for brightfield and fluorescence allow daily QC monitoring and reporting.
  • 21CFR PART11 COMPLIANCE
    COUNTWIRE™ - 21 CFR PART 11 COMPLIANCE CountWire™ software provides the complementary data security and accessibility management.

THE SOFTWARE THAT CHANGES EVERYTHING.

COUNTING MODES CUSTOMIZE & SAVE PROTOCOLS COUNT RESULTS REVIEW RESULTS REVIEW BIOPROCESS QUALITY CONTROL RESULTS

THE END TO THE ACCURACY DEBATE

The effect of cell counting volume on accuracy is often overlooked. Manual counting and most automated cell counters detect cells in a counting volume of ~ 0.5 µl. The LUNA-FX7™ utilizes a counting volume of up to 5.1 µl, lowering error and CV for each count. Improved de-clustering algorithms and precise autofocus further add to the LUNA-FX7™’s accuracy and reliability.
THE END TO THE ACCURACY DEBATE
The coefficient of variation (CV) scores are assessed through multiple independent experiments on different channel-slides 1 channel (4 exp, n = 6), 2 channels (6 exp. n = 3), 3 channels (10 exp, n = 3) and 8 channels (18 exp, n = 8).
Total concentration values of 1-ch (n = 15), 2-ch (n = 18), 3-ch (n = 30) and 8-ch (n = 40) are statistically constant . (1-way ANOVA, not significant)

EASY, FLEXIBLE AND ROBUST

Not only the simple and efficient operation via intuitive touchscreen controls, brightfield and dual fluorescent detection options, multiple counting modes, and customizable protocols allow you to easily optimize for your cell and sample type. Counting results may be exported as a CSV summary or a comprehensive PDF report with raw and tagged images. With an internal 250GB hard drive, no additional computer is needed and data may be exported via Wi-Fi, Ethernet, or USB flash drive.
EASY, FLEXIBLE AND ROBUST

UNIQUE BIOPROCESS MODE

A new bioprocess feature allows you to monitor, record, and analyze your different bioprocessing activities individually. Growth rates and trend charts may be produced that enable you to trace production and accurately forecast timelines.
UNIQUE BIOPROCESS MODE

TRUST YOUR DATA

The LUNA-FX7™ validation slides and QC software allow you to be confident and trust in your data. The brightfield and fluorescent validation slides offer exact cell concentration and viability while the QC features provide the ability to store, monitor, and analyze quality control data.
TRUST YOUR DATA

THROUGHPUT, CONTROL AND EFFICIENCY

The LUNA-FX7™ provides the ability to use multiple slide formats including 8-, 3-, 2-, and single chamber slides. The intuitive user-interface allows you to easily switch between slide formats and designate the exact chambers that are counted - allowing unused chambers to be used in the future. With ‘One and Done’ slide insertion, a slide only needs to be inserted once into the counter without having to remove and reinsert the slide to count different chambers.
THROUGHPUT, CONTROL, AND EFFICIENCY

COUNTWIRE™ - 21 CFR PART 11 COMPLIANCE

The CountWire™ System allows multiple users to remotely access and manage data from LUNA-FX7™ units, and it completely supports 21 CFR Part 11 compliance, enabling the LUNA-FX7™ Automated Cell Counter to be used in a regulated process.
 
21 CFR PART 11 COMPLIANCE
User Access Control
The CountWire™ System establishes different roles and levels of access and privilege and only authorized ones may access the system with a user ID and password. Upon successful login, they can perform only functions assigned or privileged to the role/level.
 
Electronic Signature
All reports may be digitally signed and the electronic signature information includes the name and user ID of the signer, as well as the date, time and meaning of the signature.
 
Audit Trail
All actions are recorded within the audit trail, time-stamped, tagged to the user, identified with the instrument name and serial number.
 
Data Safety and Security
The CountWire™ System reliably protects all from deletion, overwriting, alteration, and/or possible accidences.


 
APPLICATION

APPLICATION E-BOOK: Ultimate guide to automated cell counting

Counting cells: Is there a better way?
All your counting needs in one
The quest for optimal precision
The importance of cell size
CAR T: Monitoring cell health and viability
Monitor cell health and growth with precision
PBMCs: Optimize counting and viability
Meet the LUNA™ family: Tools for success

DOWNLOAD THE E-BOOK
Saloustros E, Liu S, Mertz EL, Bhattacharyya N, Starost MF, Salpea P, Nesterova M, Collins M, Leikin S, Stratakis CA.
LUNA-FX7™ Automated Cell Counter
Monitoring CAR-T Cell Therapy Production Using the LUNA-FX7™ INTRODUCTION Chimeric Antigen Receptor (CAR) T cell therapy is a combination of personalized Immunotherapy and gene1. CAR-T cell therapy is a 'living' drug established from a patient's own isolated and engineered T cells. Since the first FDA approval in 2018, CAR-T therapies have achieved 80% in remission rates for hematologic malignancy patients2. As with all adoptive cell therapies, the production of autologous or allogeneic CAR-T therapies is a complicated biomanufacturing process that requires strict adherence to regulatory and QA/QC guidelines. To be ensured the safety, quality, and efficacy of the final product, cell health and viability must be evaluated and monitored throughout the process of development and bioprocessing workflows. Here, we demonstrate how the LUNA-FX7™ may be used to monitor and evaluate cell health at critical stages in the CAR-T process: 1) Collection of the primary source material, 2) Post-Leukapheresis, 3) Isolation / Activation, 4) Expansion, and 5) Production. Curocell Inc., a leading company developing innovative anti-cancer immune therapy in Korea, kindly provided cell count data of two samples randomly selected from each stage of the CAR-T cell production (Figure 1). Cell counts were performed with the LUNA-FX7™ in fluorescent cell counting mode using the nucleic acid stains Acridine Orange and Propidium Iodide (AO/PI, Cat# F23001, Logos Biosystems). In brief, for each count, 2 µl of pre-mixed AO/PI solution was combined with 18 µl of cells, loaded into a 2-, 3-, or 8-channel slide, and counted utilizing the autofocus feature. For the final dosing determinations, the LUNA™ 1-Channel Slides (Cat# L72011) with an analysis volume of 5.1 µl (>10X volume of most automated volumes) were used. 1. Initial WBC concentration and viability from a whole blood sample After initial blood collection and prior to leukapheresis, cells were diluted 1:100 with 1X PBS for counting in LUNA-FX7™. Despite the fact that white blood cells (WBC) comprise only 2% of a whole blood population, Figure 2 shows how the LUNA-FX7™ can easily differentiate nucleated cells from anucleated cells (i.e. RBC) in a heterogeneous population. 2. Isolation of WBC by Leukapheresis Leukapheresis is the process of separating WBCs from the whole blood. Figures 3 shows the viability of 2 different samples. RBCs were not seen in either sample, but some cell debris may still be observed. Here again, the LUNA-FX7™ can easily distinguish between cells and cellular or non-cellular debris, minimizing the possibility of counting false positives. 3. T cell isolation and activation After leukapheresis, T cells are further isolated and then activated, yielding a homogenous T cell population. At this stage, there is a marked contrast in the quality of samples (Figure 4). 4. CARs expressed T cells The isolated and activated T cells are transduced through retroviruses to produce the chimeric antigen receptors (CARs). After transduction, the newly engineered CAR-T cells are expanded ex vivo. Figure 5 shows cell viability and numbers of expanded CAR-T cells. 5. CAR-T cell product Expanded CAR-T cells are cryopreserved and evaluated for appropriate dosing. Figure 6 shows the viability and concentration of 2 samples with different cell viability. CONCLUSION From the initial collection of primary source material to final dosing determinations, the LUNA-FX7™ provides the required accuracy and flexibility for monitoring cell health and viability through the entire CAR-T process. Furthermore, in conjunction with pre-set validation slides, internal QC software, and optional 21 CFR Part 11 compliant software ensures that the rigorous QC and regulatory guidelines are met and maintained. REFERENCES 1 Poorebrahim M, Sadeghi S, Fakhr E, et al. Production of CAR T-cells by GMP-grade lentiviral vectors: latest advances and future prospects. Crit Rev Clin Lab Sci. 2019;56(6):393-419. doi:10.1080/10408363.2019.1633512 2 Zhang C, Liu J, Zhong JF, Zhang X. Engineering CAR-T cells. Biomark Res. 2017;5:22. Published 2017 Jun 24. doi:10.1186/s40364-017-0102-y
Compatible Reagents
    Cat # Product Qty MSDS Product Insert
    T13001 Trypan Blue Stain, 0.4%

    2 x 1 mL

    FileAction
    T13001 Trypan Blue Stain, 0.4% MSDS.pdfDownload 
    T13001 Trypan Blue Stain, 0.4% VL1801-01.pdfDownload 
    L13002 Erythrosin B Stain

    2 x 1 mL

    FileAction
    L13002 Erythrosin B Stain MSDS.pdfDownload 
    L13002 Erythrosin B Stain VL1801-01.pdfDownload 
    F23001 Acridine Orange/Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    F23001 Acridine Orange Propidium Iodide Stain MSDS.pdfDownload 
    F23001 Acridine Orange,Propidium Iodide Stain VL1801-01.pdfDownload 
    Request CoA   >

    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Jigar Sethiya

      Creighton University

      Great item with amazing features.
      It is one of the nicest cell counters I have ever use. It is totally worth it.

    • Timo Reiß

      RWTH Aachen, Cellular and Applied Infection Biology

      Plasmodium falciparum detection.
      The LUNA system is very easy to use. The counting of erythrocytes is fast and very precise.
      The detection of plasmodium falciparum infected erythrocytes via fluorescence helps us to determine the parasitemia very fast.
      Counting using LUNA helps us to have more accurate data in less time!

    • Andile Mtshali

      CAPRISA

      It's more convenient, saves time. Very effective, minimizes errors possed by manual counts.

    • Katja Salo

      University of Helsinki

      Device has been easy to use, and simple for a new user. Autofocus works nicely which is important to us; we need consistent results because different people run cell counting as a first step of single cell analysis. Algorithm seems to work accurately as it differentiates between actual cells and cell debris or other particles. Counting with the 8-chamber slide is quite consistent, you just have to pay attention to even pipetting. Measuring with the 8-chamber slide is super easy and quick compared to Luna-stem that we used to use. Getting 8 counts and also saving all the files with two keystrokes frees you to do something else while the counter is running.

    • Emma Rogers

      OBT sciences

      The LUNA FX7 Automated Cell Counter has streamlined our workflow and increased the repeatability of our internal experiments. This equipment allows multiple users to achieve verifiable outputs and has increased our capacity at a complicated time when our personnel are limited by their in-person activities. The fluorescence and imaging capabilities of this cell counter has reduced our reliance on older, more specialized equipment. The company was responsive, engaged, and knowledgeable about the right product for our uses.

    • Karan Singh

      NYU Langone Medical Center

      It is excellent because of the following: 1-Its ease of use. 2- Reproducibility of data. 3- Take less time to process than others. 4- Reliable. 5- Value for the money.

    • Michael Schnekenburger

      LBMCC

      The device is user friendly, provide relatively fast and very accurate results. It works for all kind of cell types, adherent and suspension cell lines, CD34, PBMCs... The algorithm is doing a fantastic job to discriminate healthy from dead cells and it is amazing how it can accurately count cells even in cell aggregates. Pretty good interface to see, manage and export your data (including Wifi connection).

    • Rahul Biradar

      Turku Bioscience

      One of the most easy to use cell counters I have used so far. The quality of the images stored after counting is as good as seeing through BF microscope. Even clustered cells are well analyzed for cell counting. Higher number of fields and cells taken in to account for precise cell counting.

    show more  + + SHOW OUR CUSTOMERS

    WHERE THE LUNA-FX7™ HAS BEEN CITED

    Citations
    SHOW MORE  +

    Resources

    Downloads
    • Icon
      An analysis of optimal cell counting range of the LUNA-FX7™

      Product literature

      2021-04-07 | 721.64 KB
    • Icon
      LUNA-FX7™ software 1.5.0

      Software

      2021-01-12 | 4.51 MB
    • Icon
      Bioprocess analysis with the LUNA-FX7™

      Product literature

      2020-11-09 | 559.46 KB
    • Icon
      Incomparable cell size measurement with the LUNA-FX7™

      Product literature

      2020-11-09 | 611.17 KB
    • Icon
      The Counting Accuracy of the LUNA-FX7™ Automated Cell Counter

      Product literature

      2020-10-21 | 462.57 KB
    • Icon
      Rapid evaluation of PBMC counting and viability

      Product literature

      2020-10-21 | 578.14 KB
    • Icon
      Monitoring CAR-T Cell Therapy Production Using the LUNA-FX7™

      Product literature

      2020-10-21 | 752.75 KB
    • Icon
      LUNA-FX7™

      Brochure

      2020-10-06 | 721.36 KB
    • Icon
      Logos Biosystems Products

      Brochure

      2020-04-26 | 2.14 MB
        Videos

        Introducing the LUNA-FX7™ Automated Cell Counter

        Cell DB
          • 3T3-L1
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              4

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

            Cell Counting Results

            • Total cell concentration (cells/mL)

              9.17.E+05

            • Live cell concentration (cells/mL)

              3.22.E+05

            • Dead cell concentration (cells/mL)

              5.95.E+05

            • Viability (%)

              35.1

            • Average cell size (μm)

              15.5

          • 3T3-L1
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              7.90.E+05

            • Live cell concentration (cells/mL)

              3.39.E+05

            • Dead cell concentration (cells/mL)

              4.51.E+05

            • Viability (%)

              42.9

            • Average cell size (μm)

              14.9

          • A549
            • Min. search size(μm)

              7

            • Max. search size(μm)

              50

            • Cell detection sensitivity

              4

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

              8-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.00.E+06

            • Live cell concentration (cells/mL)

              8.78.E+05

            • Dead cell concentration (cells/mL)

              1.12.E+06

            • Viability (%)

              43.8

            • Average cell size (μm)

              17.6

          • A549
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.32.E+06

            • Live cell concentration (cells/mL)

              1.11.E+06

            • Dead cell concentration (cells/mL)

              1.21.E+06

            • Viability (%)

              47.8

            • Average cell size (μm)

              16.1

          • AsPC-1
            • Min. search size(μm)

              8

            • Max. search size(μm)

              30

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              8-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.22.E+06

            • Live cell concentration (cells/mL)

              1.13.E+06

            • Dead cell concentration (cells/mL)

              1.09.E+06

            • Viability (%)

              51

            • Average cell size (μm)

              15.5

          • AsPC-1
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              2

            • Max. cell size (μm)

              50

            • GF threshold level

              3

            • RF threshold level

              3

            • Dilution factor

              1.11

            • Size gating (μm)

              7~50

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.85.E+06

            • Live cell concentration (cells/mL)

              1.04.E+06

            • Dead cell concentration (cells/mL)

              8.12.E+05

            • Viability (%)

              56.2

            • Average cell size (μm)

              17.0

          • BAEC
            • Min. search size(μm)

              4

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.54.E+06

            • Live cell concentration (cells/mL)

              1.20.E+06

            • Dead cell concentration (cells/mL)

              1.34.E+06

            • Viability (%)

              47.3

            • Average cell size (μm)

              14.3

          • BAEC
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.49.E+06

            • Live cell concentration (cells/mL)

              1.21.E+06

            • Dead cell concentration (cells/mL)

              1.28.E+06

            • Viability (%)

              48.6

            • Average cell size (μm)

              12.9

          • Colon26L5
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              4

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.04.E+06

            • Live cell concentration (cells/mL)

              7.99.E+05

            • Dead cell concentration (cells/mL)

              1.24.E+06

            • Viability (%)

              39.2

            • Average cell size (μm)

              14.8

          • Colon26L5
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.07.E+06

            • Live cell concentration (cells/mL)

              8.22.E+05

            • Dead cell concentration (cells/mL)

              1.24.E+06

            • Viability (%)

              39.8

            • Average cell size (μm)

              13.6

          • DLD-1
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              4

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.07.E+06

            • Live cell concentration (cells/mL)

              1.03.E+06

            • Dead cell concentration (cells/mL)

              1.05.E+06

            • Viability (%)

              49.5

            • Average cell size (μm)

              13.1

          • DLD-1
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.16.E+06

            • Live cell concentration (cells/mL)

              1.13.E+06

            • Dead cell concentration (cells/mL)

              1.04.E+06

            • Viability (%)

              52.0

            • Average cell size (μm)

              12.6

          • H1975
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.19.E+06

            • Live cell concentration (cells/mL)

              6.04.E+05

            • Dead cell concentration (cells/mL)

              5.81.E+05

            • Viability (%)

              51

            • Average cell size (μm)

              15.3

          • H1975
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.08.E+06

            • Live cell concentration (cells/mL)

              6.02.E+05

            • Dead cell concentration (cells/mL)

              4.80.E+05

            • Viability (%)

              55.6

            • Average cell size (μm)

              15.3

          • HCT116
            • Min. search size(μm)

              7

            • Max. search size(μm)

              50

            • Cell detection sensitivity

              4

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.32.E+06

            • Live cell concentration (cells/mL)

              1.10.E+06

            • Dead cell concentration (cells/mL)

              1.22.E+06

            • Viability (%)

              47.3

            • Average cell size (μm)

              16

          • HCT116
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              4

            • Max. cell size (μm)

              50

            • GF threshold level

              3

            • RF threshold level

              3

            • Dilution factor

              1.11

            • Size gating (μm)

              7~50

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.16.E+06

            • Live cell concentration (cells/mL)

              1.09.E+06

            • Dead cell concentration (cells/mL)

              1.08.E+06

            • Viability (%)

              50.2

            • Average cell size (μm)

              14.8

          • Hek-293
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              4

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.88.E+06

            • Live cell concentration (cells/mL)

              1.38.E+06

            • Dead cell concentration (cells/mL)

              1.50.E+06

            • Viability (%)

              48

            • Average cell size (μm)

              14.2

          • Hek-293
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              1

            • Max. cell size (μm)

              30

            • GF threshold level

              2

            • RF threshold level

              2

            • Dilution factor

              1.11

            • Size gating (μm)

              7~30

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.86.E+06

            • Live cell concentration (cells/mL)

              1.18.E+06

            • Dead cell concentration (cells/mL)

              1.68.E+06

            • Viability (%)

              41.3

            • Average cell size (μm)

              12.6

          • HL60
            • Min. search size(μm)

              7

            • Max. search size(μm)

              23

            • Cell detection sensitivity

              3

            • Live cell sensitivity

              4

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.72.E+06

            • Live cell concentration (cells/mL)

              1.43.E+06

            • Dead cell concentration (cells/mL)

              1.29.E+06

            • Viability (%)

              52.7

            • Average cell size (μm)

              12.5

          • HL60
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              3.02.E+06

            • Live cell concentration (cells/mL)

              1.57.E+06

            • Dead cell concentration (cells/mL)

              1.44.E+06

            • Viability (%)

              52.2

            • Average cell size (μm)

              11.1

          • J774A.1
            • Min. search size(μm)

              4

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              4

            • Noise reduction

              6

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.12.E+06

            • Live cell concentration (cells/mL)

              5.31.E+05

            • Dead cell concentration (cells/mL)

              5.93.E+05

            • Viability (%)

              47.2

            • Average cell size (μm)

              14.5

          • J774A.1
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              8.94.E+05

            • Live cell concentration (cells/mL)

              4.56.E+05

            • Dead cell concentration (cells/mL)

              4.38.E+05

            • Viability (%)

              51

            • Average cell size (μm)

              14.3

          • RAW264.7
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              4.27.E+06

            • Live cell concentration (cells/mL)

              1.65.E+06

            • Dead cell concentration (cells/mL)

              2.62.E+06

            • Viability (%)

              38.6

            • Average cell size (μm)

              11.9

          • RAW264.7
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              3.76.E+06

            • Live cell concentration (cells/mL)

              1.38.E+06

            • Dead cell concentration (cells/mL)

              2.39.E+06

            • Viability (%)

              36.5

            • Average cell size (μm)

              12.2

          • SY5Y
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              3.32.E+06

            • Live cell concentration (cells/mL)

              1.65.E+06

            • Dead cell concentration (cells/mL)

              1.66.E+06

            • Viability (%)

              49.9

            • Average cell size (μm)

              14.4

          • SY5Y
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~70

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.21.E+06

            • Live cell concentration (cells/mL)

              5.55.E+05

            • Dead cell concentration (cells/mL)

              6.60.E+05

            • Viability (%)

              45.7

            • Average cell size (μm)

              14.9

          • U2OS
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.49.E+06

            • Live cell concentration (cells/mL)

              6.78.E+05

            • Dead cell concentration (cells/mL)

              8.08.E+05

            • Viability (%)

              48.6

            • Average cell size (μm)

              15.9

          • U2OS
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              2

            • Max. cell size (μm)

              50

            • GF threshold level

              3

            • RF threshold level

              3

            • Dilution factor

              1.11

            • Size gating (μm)

              7~50

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.46.E+06

            • Live cell concentration (cells/mL)

              7.09.E+05

            • Dead cell concentration (cells/mL)

              7.52.E+05

            • Viability (%)

              48.5

            • Average cell size (μm)

              16.7

          • U937
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              3.27.E+06

            • Live cell concentration (cells/mL)

              1.58.E+06

            • Dead cell concentration (cells/mL)

              1.69.E+06

            • Viability (%)

              48.2

            • Average cell size (μm)

              12.5

          • U937
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              2

            • Max. cell size (μm)

              20

            • GF threshold level

              8

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

              7~20

            Cell Counting Results

            • Total cell concentration (cells/mL)

              3.11.E+06

            • Live cell concentration (cells/mL)

              1.48.E+06

            • Dead cell concentration (cells/mL)

              1.63.E+06

            • Viability (%)

              47.6

            • Average cell size (μm)

              11.5

          • K562
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              2.25.E+06

            • Live cell concentration (cells/mL)

              1.08.E+06

            • Dead cell concentration (cells/mL)

              1.17.E+06

            • Viability (%)

              48

            • Average cell size (μm)

              14.6

          • PBMC
            • GF exposure level

              5

            • RF exposure level

              5

            • Min. cell size (μm)

              2

            • Max. cell size (μm)

              50

            • GF threshold level

              4

            • RF threshold level

              4

            • Dilution factor

              1.11

            • Size gating (μm)

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.42.E+06

            • Live cell concentration (cells/mL)

              4.96.E+05

            • Dead cell concentration (cells/mL)

              9.20.E+06

            • Viability (%)

              35.0

            • Average cell size (μm)

              7.1

          • M1
            • Min. search size(μm)

              7

            • Max. search size(μm)

              24

            • Cell detection sensitivity

              5

            • Live cell sensitivity

              5

            • Noise reduction

              5

            • Dilution factor

              2

            • Size gating(μm)

              7-90

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.86.E+06

            • Live cell concentration (cells/mL)

              9.69.E+05

            • Dead cell concentration (cells/mL)

              8.92.E+05

            • Viability (%)

              52.1

            • Average cell size (μm)

              10.7

          • Fluorescence bead : 12um, Red
            • GF exposure level

              3

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

            Cell Counting Results

            • Total cell concentration (cells/mL)

              9.37.E+05

            • Live cell concentration (cells/mL)

              0.00.E+00

            • Dead cell concentration (cells/mL)

              9.37.E+05

            • Viability (%)

              0

            • Average cell size (μm)

              10.5

          • Fluorescence bead : 12um, Green
            • GF exposure level

              3

            • RF exposure level

              5

            • Min. cell size (μm)

              3

            • Max. cell size (μm)

              70

            • GF threshold level

              5

            • RF threshold level

              5

            • Dilution factor

              1.11

            • Size gating (μm)

            Cell Counting Results

            • Total cell concentration (cells/mL)

              1.10.E+06

            • Live cell concentration (cells/mL)

              1.10.E+06

            • Dead cell concentration (cells/mL)

              0.00.E+00

            • Viability (%)

              100

            • Average cell size (μm)

              11.2

        show more  +

        ORDERING INFORMATION

        Cat # Product Qty
        L70001 LUNA-FX7™ Automated Cell Counter, Basic Package 1 unit
        L70002 LUNA-FX7™ Automated Cell Counter, Advanced Package 1 unit
        L72011 LUNA™ 1-Channel Slides, 50 Slides 1 box
        L72012 LUNA™ 1-Channel Slides, 500 Slides 10 boxes
        L72013 LUNA™ 1-Channel Slides, Sterile - gamma-irradiated, 500 Slides 10 boxes
        L72021 LUNA™ 3-Channel Slides, 50 Slides 1 box
        L72022 LUNA™ 3-Channel Slides, 500 Slides 10 boxes
        L72023 LUNA™ 3-Channel Slides, Sterile - gamma-irradiated, 500 Slides 10 boxes
        L72001 LUNA™ 8-Channel Slides, 50 Slides 1 box
        L72002 LUNA™ 8-Channel Slides, 500 Slides 10 boxes
        L72003 LUNA™ 8-Channel Slides, Sterile - gamma-irradiated, 500 Slides 10 boxes
        L12005 PhotonSlide™, 50 Slides 1 box
        L12006 PhotonSlide™, 500 Slides 10 boxes
        L12007 PhotonSlide™, 1000 Slides 20 boxes
        L12001 LUNA™ Cell Counting Slides, 50 Slides 1 box
        L12002 LUNA™ Cell Counting Slides, 500 Slides 10 boxes
        L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 boxes
        L72040 Cell Counter Validation Slide-BF 1 unit
        L72030 Cell Counter Validation Slide-FL 1 unit
        F73101 LUNA-FX™ Calibration Beads Kit 2 x 0.5 mL
        T13001 Trypan Blue Stain, 0.4% 2 X 1 mL
        T13011 Trypan Blue Stain, 0.4%, Sterile-filtered 2 X 1 mL
        L13002 Erythrosin B Stain 2 x 1 mL
        F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
        F23011 Acridine Orange/Propidium Iodide Stain, Sterile-filtered 2 x 0.5 mL
        L71001 CountWire™ Basic
        (1 CountWire™ Data Storage + 1 CountWire™ Verification Key)
        1 set
        L71002 CountWire™ Verification Key (additional) 1 unit
        L74003 LUNA-FX7™ IQ/OQ Protocol 1 copy
        P17001 Thermal Printer for LUNA-FX™ 1 unit
        P17002 Thermal Printer Refills, Paper and Ribbon for LUNA-FX™ 2 x 5 rolls