LUNA-FX7™ Automated Cell Counter

Introducing the LUNA-FX7™ - the newest member of the award-winning LUNA™ Cell Counter family. The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, new 1, 3 and 8-chamber slide options, state of the art optics, dual fluorescent and brightfield detection, precision autofocus, and 21 CFR Part 11 compliance. The LUNA-FX7™ has built-in quality control software and new validation slides for monitoring QC and production. By combining the trademark ease of use characteristic of all LUNA™ counters with the enhanced accuracy, higher throughput, and software upgrades - the LUNA-FX7™ will improve the efficiency and security of your lab's workflow.

WHAT THE LUNA-FX7™ DOES BEST

BEST IN CLASS ACCURACY Improved counting algorithm and increased measuring volumes lower the CV, even less than 1%!
MULTIPLE SAMPLES IN ONE GO New 8- and 3-chamber slide increase the sample throughput.
EXPANDED CELL CONCENTRATION RANGE (1.0E04 – 5.0E07 CELLS/ML) Suitable for diverse cell counting applications including single cell sequencing and CAR-T dosing determinations.
EXCELLENCE OF COUNTING BOTH BRIGHTFIELD AND FLUORESCENCE The fast and precise autofocus with an optimized software algorithm fulfils desired counting results.
CONFIDENCE, TRUST AND RELIABILITY Built-in QC software and validation slides for brightfield and fluorescence allow daily QC monitoring and reporting.
COUNTWIRE™ - 21 CFR PART 11 COMPLIANCE CountWire™ software provides the complementary data security and accessibility management.

THE SOFTWARE THAT CHANGES EVERYTHING.

THE END TO THE ACCURACY DEBATE

The effect of cell counting volume on accuracy is often overlooked. Manual counting and most automated cell counters detect cells in a counting volume of ~ 0.5 µl. The LUNA-FX7™ utilizes a counting volume of up to 5.1 µl, lowering error and CV for each count. Improved de-clustering algorithms and precise autofocus further add to the LUNA-FX7™’s accuracy and reliability.

The coefficient of variation (CV) scores are assessed through multiple independent experiments on different channel-slides 1 channel (4 exp, n = 6), 2 channels (6 exp. n = 3), 3 channels (10 exp, n = 3) and 8 channels (18 exp, n = 8).

Total concentration values of 1-ch (n = 15), 2-ch (n = 18), 3-ch (n = 30) and 8-ch (n = 40) are statistically constant . (1-way ANOVA, not significant)

EASY, FLEXIBLE AND ROBUST

Not only the simple and efficient operation via intuitive touchscreen controls, brightfield and dual fluorescent detection options, multiple counting modes, and customizable protocols allow you to easily optimize for your cell and sample type. Counting results may be exported as a CSV summary or a comprehensive PDF report with raw and tagged images. With an internal 250GB hard drive, no additional computer is needed and data may be exported via Wi-Fi, Ethernet, or USB flash drive.

UNIQUE BIOPROCESS MODE

A new bioprocess feature allows you to monitor, record, and analyze your different bioprocessing activities individually. Growth rates and trend charts may be produced that enable you to trace production and accurately forecast timelines.

TRUST YOUR DATA

The LUNA-FX7™ validation slides and QC software allow you to be confident and trust in your data. The brightfield and fluorescent validation slides offer exact cell concentration and viability while the QC features provide the ability to store, monitor, and analyze quality control data.

THROUGHPUT, CONTROL AND EFFICIENCY

The LUNA-FX7™ provides the ability to use multiple slide formats including 8-, 3-, 2-, and single chamber slides. The intuitive user-interface allows you to easily switch between slide formats and designate the exact chambers that are counted - allowing unused chambers to be used in the future. With ‘One and Done’ slide insertion, a slide only needs to be inserted once into the counter without having to remove and reinsert the slide to count different chambers.

COUNTWIRE™ - 21 CFR PART 11 COMPLIANCE

The CountWire™ System allows multiple users to remotely access and manage data from LUNA-FX7™ units, and it completely supports 21 CFR Part 11 compliance, enabling the LUNA-FX7™ Automated Cell Counter to be used in a regulated process.

User Access Control

The CountWire™ System establishes different roles and levels of access and privilege and only authorized ones may access the system with a user ID and password. Upon successful login, they can perform only functions assigned or privileged to the role/level.

Electronic Signature

All reports may be digitally signed and the electronic signature information includes the name and user ID of the signer, as well as the date, time and meaning of the signature.

Audit Trail

All actions are recorded within the audit trail, time-stamped, tagged to the user, identified with the instrument name and serial number.

Data Safety and Security

The CountWire™ System reliably protects all from deletion, overwriting, alteration, and/or possible accidences.

Whitepaper

APPLICATION

APPLICATION E-BOOK: Ultimate guide to automated cell counting

Counting cells: Is there a better way? All your counting needs in one The quest for optimal precision The importance of cell size CAR T: Monitoring cell health and viability Monitor cell health and growth with precision PBMCs: Optimize counting and viability Meet the LUNA™ family: Tools for success

DOWNLOAD THE E-BOOK

Saloustros E, Liu S, Mertz EL, Bhattacharyya N, Starost MF, Salpea P, Nesterova M, Collins M, Leikin S, Stratakis CA.

LUNA-FX7™ Automated Cell Counter

Monitoring CAR-T Cell Therapy Production Using the LUNA-FX7™ INTRODUCTION Chimeric Antigen Receptor (CAR) T cell therapy is a combination of personalized Immunotherapy and gene1. CAR-T cell therapy is a 'living' drug established from a patient's own isolated and engineered T cells. Since the first FDA approval in 2018, CAR-T therapies have achieved 80% in remission rates for hematologic malignancy patients2. As with all adoptive cell therapies, the production of autologous or allogeneic CAR-T therapies is a complicated biomanufacturing process that requires strict adherence to regulatory and QA/QC guidelines. To be ensured the safety, quality, and efficacy of the final product, cell health and viability must be evaluated and monitored throughout the process of development and bioprocessing workflows. Here, we demonstrate how the LUNA-FX7™ may be used to monitor and evaluate cell health at critical stages in the CAR-T process: 1) Collection of the primary source material, 2) Post-Leukapheresis, 3) Isolation / Activation, 4) Expansion, and 5) Production. Curocell Inc., a leading company developing innovative anti-cancer immune therapy in Korea, kindly provided cell count data of two samples randomly selected from each stage of the CAR-T cell production (Figure 1). Cell counts were performed with the LUNA-FX7™ in fluorescent cell counting mode using the nucleic acid stains Acridine Orange and Propidium Iodide (AO/PI, Cat# F23001, Logos Biosystems). In brief, for each count, 2 µl of pre-mixed AO/PI solution was combined with 18 µl of cells, loaded into a 2-, 3-, or 8-channel slide, and counted utilizing the autofocus feature. For the final dosing determinations, the LUNA™ 1-Channel Slides (Cat# L72011) with an analysis volume of 5.1 µl (>10X volume of most automated volumes) were used. 1. Initial WBC concentration and viability from a whole blood sample After initial blood collection and prior to leukapheresis, cells were diluted 1:100 with 1X PBS for counting in LUNA-FX7™. Despite the fact that white blood cells (WBC) comprise only 2% of a whole blood population, Figure 2 shows how the LUNA-FX7™ can easily differentiate nucleated cells from anucleated cells (i.e. RBC) in a heterogeneous population. 2. Isolation of WBC by Leukapheresis Leukapheresis is the process of separating WBCs from the whole blood. Figures 3 shows the viability of 2 different samples. RBCs were not seen in either sample, but some cell debris may still be observed. Here again, the LUNA-FX7™ can easily distinguish between cells and cellular or non-cellular debris, minimizing the possibility of counting false positives. 3. T cell isolation and activation After leukapheresis, T cells are further isolated and then activated, yielding a homogenous T cell population. At this stage, there is a marked contrast in the quality of samples (Figure 4). 4. CARs expressed T cells The isolated and activated T cells are transduced through retroviruses to produce the chimeric antigen receptors (CARs). After transduction, the newly engineered CAR-T cells are expanded ex vivo. Figure 5 shows cell viability and numbers of expanded CAR-T cells. 5. CAR-T cell product Expanded CAR-T cells are cryopreserved and evaluated for appropriate dosing. Figure 6 shows the viability and concentration of 2 samples with different cell viability. CONCLUSION From the initial collection of primary source material to final dosing determinations, the LUNA-FX7™ provides the required accuracy and flexibility for monitoring cell health and viability through the entire CAR-T process. Furthermore, in conjunction with pre-set validation slides, internal QC software, and optional 21 CFR Part 11 compliant software ensures that the rigorous QC and regulatory guidelines are met and maintained. REFERENCES 1 Poorebrahim M, Sadeghi S, Fakhr E, et al. Production of CAR T-cells by GMP-grade lentiviral vectors: latest advances and future prospects. Crit Rev Clin Lab Sci. 2019;56(6):393-419. doi:10.1080/10408363.2019.1633512 2 Zhang C, Liu J, Zhong JF, Zhang X. Engineering CAR-T cells. Biomark Res. 2017;5:22. Published 2017 Jun 24. doi:10.1186/s40364-017-0102-y

Compatible Reagents

Cat #

Product

Qty

MSDS

Product Insert

T13001

Trypan Blue Stain, 0.4%

2 x 1 mL

File	Action
T13001 Trypan Blue Stain, 0.4% MSDS.pdf	Download
T13001 Trypan Blue Stain, 0.4% VL1801-01.pdf	Download

L13002

Erythrosin B Stain

2 x 1 mL

File	Action
L13002 Erythrosin B Stain MSDS.pdf	Download
L13002 Erythrosin B Stain VL1801-01.pdf	Download

F23001

Acridine Orange/Propidium Iodide Stain

2 x 0.5 mL

File	Action
F23001 Acridine Orange Propidium Iodide Stain MSDS.pdf	Download
F23001 Acridine Orange,Propidium Iodide Stain VL1801-01.pdf	Download

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WHAT DO OUR CUSTOMERS HAVE TO SAY?

Testimonials

Matthew Wong
Flagship Pioneering

Great product! Great product. Reliable and easy to use.
Jared Sills
Vertex

Great results that are accurate all the time.
The LUNA-FX7 has made my life so much easier! Compared to hemocytometer counting, it is way more efficient and time saving. Our entire team has loved the ease of use as well. The option to use Bright field or Fluorescence is very handy in our field.
Devin Mair
Johns Hopkins University

Accurate counts for hard to count cell types.
We primarily do cardiomyocyte differentiations from iPSCs. This system, with the Acridine Orange/Propidium iodide stain, lets us count cardiomyocytes with much more accuracy than our previous Countess system. This has greatly enahnced our quality control and the reproducibility of the experiments. Software is easy to use. Very nice piece of equipment, if a little on the expensive side. Overall, the improvements are worth the price.
Syed Shah
Baylor College of Medicine

Overall, extremely satisfied with the product.
The primary reason for the purchase of LUNA-FX7 was for us to count the number of cells in different parts of the cytoplasm, and we were able to achieve that quite easily.
Neal Shekar
Ragon Institute

Good product. Great product very nice to use.
Sharbari Das
ABC Labs

LUNA is a lifesaver.
Perfect and great fit as the instrument has a unique design. Its user friendly manual helps to count even large number of cells effortlessly. Most importantly, it saves your valuable time. Press the button and you're done. Smooth and efficient at the same time.
Pete Davis

Awesome results = Awesome science.
Turn it on and it is ready to go. Tested it against 3 other systems on the same cells... spot on results. Tech support helped me with great application solutions.
BINGYING HAN

Easy to use and accurate results.
The LUNA-FX7™ Automated Cell Counter is easy to use. It can count cells very quickly and accurately. The customer service response is fast and knowledgeable. It's good value for the money.
Yahya Nuruddeen
Avalon Pharma

Excellent!
The LUNA FX7 Automated Cell Counter has eased the workflow and focused heavily on the repeatability of experimental results. Achieving high- throughput efficiency in the outcome of studies. The fluorescence and imaging capabilities have brought about a technology shift from older ways. The company have a dedicated team and is knowledgeable about the right product for our uses.
Jigar Sethiya
Creighton University

Great item with amazing features.
It is one of the nicest cell counters I have ever use. It is totally worth it.
Timo Reiß

Plasmodium falciparum detection.
The LUNA system is very easy to use. The counting of erythrocytes is fast and very precise.
The detection of plasmodium falciparum infected erythrocytes via fluorescence helps us to determine the parasitemia very fast.
Counting using LUNA helps us to have more accurate data in less time!
Andile Mtshali

It's more convenient, saves time. Very effective, minimizes errors possed by manual counts.
Katja Salo

Device has been easy to use, and simple for a new user. Autofocus works nicely which is important to us; we need consistent results because different people run cell counting as a first step of single cell analysis. Algorithm seems to work accurately as it differentiates between actual cells and cell debris or other particles. Counting with the 8-chamber slide is quite consistent, you just have to pay attention to even pipetting. Measuring with the 8-chamber slide is super easy and quick compared to Luna-stem that we used to use. Getting 8 counts and also saving all the files with two keystrokes frees you to do something else while the counter is running.
Emma Rogers

The LUNA FX7 Automated Cell Counter has streamlined our workflow and increased the repeatability of our internal experiments. This equipment allows multiple users to achieve verifiable outputs and has increased our capacity at a complicated time when our personnel are limited by their in-person activities. The fluorescence and imaging capabilities of this cell counter has reduced our reliance on older, more specialized equipment. The company was responsive, engaged, and knowledgeable about the right product for our uses.
Karan Singh
NYU Langone Medical Center

It is excellent because of the following: 1-Its ease of use. 2- Reproducibility of data. 3- Take less time to process than others. 4- Reliable. 5- Value for the money.
Michael Schnekenburger
LBMCC

The device is user friendly, provide relatively fast and very accurate results. It works for all kind of cell types, adherent and suspension cell lines, CD34, PBMCs... The algorithm is doing a fantastic job to discriminate healthy from dead cells and it is amazing how it can accurately count cells even in cell aggregates. Pretty good interface to see, manage and export your data (including Wifi connection).
Rahul Biradar

One of the most easy to use cell counters I have used so far. The quality of the images stored after counting is as good as seeing through BF microscope. Even clustered cells are well analyzed for cell counting. Higher number of fields and cells taken in to account for precise cell counting.

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WHERE THE LUNA-FX7™ HAS BEEN CITED

Citations

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Resources

Downloads

LUNA-FX7™ - CountWire

Whitepaper

2021-08-24 | 2.30 MB

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LUNA-FX7™

Quick Start Guide

2021-08-16 | 432.07 KB

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LUNA-FX7™

brochure

2021-05-21 | 1.42 MB

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Logos Biosystems Products

Brochure

2020-04-26 | 2.14 MB

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Yeast Cell Counting using the LUNA-FX7™ Automated Cell Counter

Application note

2022-01-21 | 2.42 MB

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Automated Cell Evaluation for Single-Cell RNA-seq Analysis

Application note

2021-06-17 | 1.83 MB

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An analysis of optimal cell counting range of the LUNA-FX7™

Application note

2021-04-07 | 721.64 KB

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Bioprocess analysis with the LUNA-FX7™

Application note

2020-11-09 | 559.46 KB

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Incomparable cell size measurement with the LUNA-FX7™

Application note

2020-11-09 | 611.17 KB

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The Counting Accuracy of the LUNA-FX7™ Automated Cell Counter

Application note

2020-10-21 | 462.57 KB

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Rapid evaluation of PBMC counting and viability

Application note

2020-10-21 | 578.14 KB

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Monitoring CAR-T Cell Therapy Production Using the LUNA-FX7™

Application note

2020-10-21 | 752.75 KB

download

Videos

Introducing the LUNA-FX7™ Automated Cell Counter

CAR-T Cell Production and Cell Counting with the LUNA-FX7™

Introducing the LUNA-FX7™ Automated Cell Counter

2020-07-27

CAR-T Cell Production and Cell Counting with the LUNA-FX7™

2020-06-30

Cell DB

- 3T3-L1
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    4
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    9.17.E+05
  - Live cell concentration (cells/mL)
    
    3.22.E+05
  - Dead cell concentration (cells/mL)
    
    5.95.E+05
  - Viability (%)
    
    35.1
  - Average cell size (μm)
    
    15.5
- 3T3-L1
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    7.90.E+05
  - Live cell concentration (cells/mL)
    
    3.39.E+05
  - Dead cell concentration (cells/mL)
    
    4.51.E+05
  - Viability (%)
    
    42.9
  - Average cell size (μm)
    
    14.9
- A549
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    50
  - Cell detection sensitivity
    
    4
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
    
    8-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.00.E+06
  - Live cell concentration (cells/mL)
    
    8.78.E+05
  - Dead cell concentration (cells/mL)
    
    1.12.E+06
  - Viability (%)
    
    43.8
  - Average cell size (μm)
    
    17.6
- A549
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.32.E+06
  - Live cell concentration (cells/mL)
    
    1.11.E+06
  - Dead cell concentration (cells/mL)
    
    1.21.E+06
  - Viability (%)
    
    47.8
  - Average cell size (μm)
    
    16.1
- AsPC-1
  - Min. search size(μm)
    
    8
  - Max. search size(μm)
    
    30
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    8-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.22.E+06
  - Live cell concentration (cells/mL)
    
    1.13.E+06
  - Dead cell concentration (cells/mL)
    
    1.09.E+06
  - Viability (%)
    
    51
  - Average cell size (μm)
    
    15.5
- AsPC-1
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    2
  - Max. cell size (μm)
    
    50
  - GF threshold level
    
    3
  - RF threshold level
    
    3
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~50
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.85.E+06
  - Live cell concentration (cells/mL)
    
    1.04.E+06
  - Dead cell concentration (cells/mL)
    
    8.12.E+05
  - Viability (%)
    
    56.2
  - Average cell size (μm)
    
    17.0
- BAEC
  - Min. search size(μm)
    
    4
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.54.E+06
  - Live cell concentration (cells/mL)
    
    1.20.E+06
  - Dead cell concentration (cells/mL)
    
    1.34.E+06
  - Viability (%)
    
    47.3
  - Average cell size (μm)
    
    14.3
- BAEC
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.49.E+06
  - Live cell concentration (cells/mL)
    
    1.21.E+06
  - Dead cell concentration (cells/mL)
    
    1.28.E+06
  - Viability (%)
    
    48.6
  - Average cell size (μm)
    
    12.9
- Colon26L5
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    4
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.04.E+06
  - Live cell concentration (cells/mL)
    
    7.99.E+05
  - Dead cell concentration (cells/mL)
    
    1.24.E+06
  - Viability (%)
    
    39.2
  - Average cell size (μm)
    
    14.8
- Colon26L5
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.07.E+06
  - Live cell concentration (cells/mL)
    
    8.22.E+05
  - Dead cell concentration (cells/mL)
    
    1.24.E+06
  - Viability (%)
    
    39.8
  - Average cell size (μm)
    
    13.6
- DLD-1
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    4
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.07.E+06
  - Live cell concentration (cells/mL)
    
    1.03.E+06
  - Dead cell concentration (cells/mL)
    
    1.05.E+06
  - Viability (%)
    
    49.5
  - Average cell size (μm)
    
    13.1
- DLD-1
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.16.E+06
  - Live cell concentration (cells/mL)
    
    1.13.E+06
  - Dead cell concentration (cells/mL)
    
    1.04.E+06
  - Viability (%)
    
    52.0
  - Average cell size (μm)
    
    12.6
- H1975
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.19.E+06
  - Live cell concentration (cells/mL)
    
    6.04.E+05
  - Dead cell concentration (cells/mL)
    
    5.81.E+05
  - Viability (%)
    
    51
  - Average cell size (μm)
    
    15.3
- H1975
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.08.E+06
  - Live cell concentration (cells/mL)
    
    6.02.E+05
  - Dead cell concentration (cells/mL)
    
    4.80.E+05
  - Viability (%)
    
    55.6
  - Average cell size (μm)
    
    15.3
- HCT116
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    50
  - Cell detection sensitivity
    
    4
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.32.E+06
  - Live cell concentration (cells/mL)
    
    1.10.E+06
  - Dead cell concentration (cells/mL)
    
    1.22.E+06
  - Viability (%)
    
    47.3
  - Average cell size (μm)
    
    16
- HCT116
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    4
  - Max. cell size (μm)
    
    50
  - GF threshold level
    
    3
  - RF threshold level
    
    3
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~50
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.16.E+06
  - Live cell concentration (cells/mL)
    
    1.09.E+06
  - Dead cell concentration (cells/mL)
    
    1.08.E+06
  - Viability (%)
    
    50.2
  - Average cell size (μm)
    
    14.8
- Hek-293
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    4
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.88.E+06
  - Live cell concentration (cells/mL)
    
    1.38.E+06
  - Dead cell concentration (cells/mL)
    
    1.50.E+06
  - Viability (%)
    
    48
  - Average cell size (μm)
    
    14.2
- Hek-293
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    1
  - Max. cell size (μm)
    
    30
  - GF threshold level
    
    2
  - RF threshold level
    
    2
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~30
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.86.E+06
  - Live cell concentration (cells/mL)
    
    1.18.E+06
  - Dead cell concentration (cells/mL)
    
    1.68.E+06
  - Viability (%)
    
    41.3
  - Average cell size (μm)
    
    12.6
- HL60
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    23
  - Cell detection sensitivity
    
    3
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.72.E+06
  - Live cell concentration (cells/mL)
    
    1.43.E+06
  - Dead cell concentration (cells/mL)
    
    1.29.E+06
  - Viability (%)
    
    52.7
  - Average cell size (μm)
    
    12.5
- HL60
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    3.02.E+06
  - Live cell concentration (cells/mL)
    
    1.57.E+06
  - Dead cell concentration (cells/mL)
    
    1.44.E+06
  - Viability (%)
    
    52.2
  - Average cell size (μm)
    
    11.1
- J774A.1
  - Min. search size(μm)
    
    4
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    4
  - Noise reduction
    
    6
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.12.E+06
  - Live cell concentration (cells/mL)
    
    5.31.E+05
  - Dead cell concentration (cells/mL)
    
    5.93.E+05
  - Viability (%)
    
    47.2
  - Average cell size (μm)
    
    14.5
- J774A.1
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    8.94.E+05
  - Live cell concentration (cells/mL)
    
    4.56.E+05
  - Dead cell concentration (cells/mL)
    
    4.38.E+05
  - Viability (%)
    
    51
  - Average cell size (μm)
    
    14.3
- RAW264.7
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    4.27.E+06
  - Live cell concentration (cells/mL)
    
    1.65.E+06
  - Dead cell concentration (cells/mL)
    
    2.62.E+06
  - Viability (%)
    
    38.6
  - Average cell size (μm)
    
    11.9
- RAW264.7
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    3.76.E+06
  - Live cell concentration (cells/mL)
    
    1.38.E+06
  - Dead cell concentration (cells/mL)
    
    2.39.E+06
  - Viability (%)
    
    36.5
  - Average cell size (μm)
    
    12.2
- SY5Y
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    3.32.E+06
  - Live cell concentration (cells/mL)
    
    1.65.E+06
  - Dead cell concentration (cells/mL)
    
    1.66.E+06
  - Viability (%)
    
    49.9
  - Average cell size (μm)
    
    14.4
- SY5Y
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~70
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.21.E+06
  - Live cell concentration (cells/mL)
    
    5.55.E+05
  - Dead cell concentration (cells/mL)
    
    6.60.E+05
  - Viability (%)
    
    45.7
  - Average cell size (μm)
    
    14.9
- U2OS
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.49.E+06
  - Live cell concentration (cells/mL)
    
    6.78.E+05
  - Dead cell concentration (cells/mL)
    
    8.08.E+05
  - Viability (%)
    
    48.6
  - Average cell size (μm)
    
    15.9
- U2OS
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    2
  - Max. cell size (μm)
    
    50
  - GF threshold level
    
    3
  - RF threshold level
    
    3
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~50
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.46.E+06
  - Live cell concentration (cells/mL)
    
    7.09.E+05
  - Dead cell concentration (cells/mL)
    
    7.52.E+05
  - Viability (%)
    
    48.5
  - Average cell size (μm)
    
    16.7
- U937
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    3.27.E+06
  - Live cell concentration (cells/mL)
    
    1.58.E+06
  - Dead cell concentration (cells/mL)
    
    1.69.E+06
  - Viability (%)
    
    48.2
  - Average cell size (μm)
    
    12.5
- U937
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    2
  - Max. cell size (μm)
    
    20
  - GF threshold level
    
    8
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
    
    7~20
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    3.11.E+06
  - Live cell concentration (cells/mL)
    
    1.48.E+06
  - Dead cell concentration (cells/mL)
    
    1.63.E+06
  - Viability (%)
    
    47.6
  - Average cell size (μm)
    
    11.5
- K562
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    2.25.E+06
  - Live cell concentration (cells/mL)
    
    1.08.E+06
  - Dead cell concentration (cells/mL)
    
    1.17.E+06
  - Viability (%)
    
    48
  - Average cell size (μm)
    
    14.6
- PBMC
  - GF exposure level
    
    5
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    2
  - Max. cell size (μm)
    
    50
  - GF threshold level
    
    4
  - RF threshold level
    
    4
  - Dilution factor
    
    1.11
  - Size gating (μm)
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.42.E+06
  - Live cell concentration (cells/mL)
    
    4.96.E+05
  - Dead cell concentration (cells/mL)
    
    9.20.E+06
  - Viability (%)
    
    35.0
  - Average cell size (μm)
    
    7.1
- M1
  - Min. search size(μm)
    
    7
  - Max. search size(μm)
    
    24
  - Cell detection sensitivity
    
    5
  - Live cell sensitivity
    
    5
  - Noise reduction
    
    5
  - Dilution factor
    
    2
  - Size gating(μm)
    
    7-90
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.86.E+06
  - Live cell concentration (cells/mL)
    
    9.69.E+05
  - Dead cell concentration (cells/mL)
    
    8.92.E+05
  - Viability (%)
    
    52.1
  - Average cell size (μm)
    
    10.7
- Fluorescence bead : 12um, Red
  - GF exposure level
    
    3
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    9.37.E+05
  - Live cell concentration (cells/mL)
    
    0.00.E+00
  - Dead cell concentration (cells/mL)
    
    9.37.E+05
  - Viability (%)
    
    0
  - Average cell size (μm)
    
    10.5
- Fluorescence bead : 12um, Green
  - GF exposure level
    
    3
  - RF exposure level
    
    5
  - Min. cell size (μm)
    
    3
  - Max. cell size (μm)
    
    70
  - GF threshold level
    
    5
  - RF threshold level
    
    5
  - Dilution factor
    
    1.11
  - Size gating (μm)
  Cell Counting Results
  - Total cell concentration (cells/mL)
    
    1.10.E+06
  - Live cell concentration (cells/mL)
    
    1.10.E+06
  - Dead cell concentration (cells/mL)
    
    0.00.E+00
  - Viability (%)
    
    100
  - Average cell size (μm)
    
    11.2

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ORDERING INFORMATION

Cat #	Product	Qty
L70001	LUNA-FX7™ Automated Cell Counter, Basic Package	1 unit
L70002	LUNA-FX7™ Automated Cell Counter, Advanced Package	1 unit
L72011	LUNA™ 1-Channel Slides, 50 Slides	1 box
L72012	LUNA™ 1-Channel Slides, 500 Slides	10 boxes
L72013	LUNA™ 1-Channel Slides, Sterile - gamma-irradiated, 500 Slides	10 boxes
L72021	LUNA™ 3-Channel Slides, 50 Slides	1 box
L72022	LUNA™ 3-Channel Slides, 500 Slides	10 boxes
L72023	LUNA™ 3-Channel Slides, Sterile - gamma-irradiated, 500 Slides	10 boxes
L72001	LUNA™ 8-Channel Slides, 50 Slides	1 box
L72002	LUNA™ 8-Channel Slides, 500 Slides	10 boxes
L72003	LUNA™ 8-Channel Slides, Sterile - gamma-irradiated, 500 Slides	10 boxes
L12005	PhotonSlide™, 50 Slides	1 box
L12006	PhotonSlide™, 500 Slides	10 boxes
L12007	PhotonSlide™, 1000 Slides	20 boxes
L12001	LUNA™ Cell Counting Slides, 50 Slides	1 box
L12002	LUNA™ Cell Counting Slides, 500 Slides	10 boxes
L12003	LUNA™ Cell Counting Slides, 1000 Slides	20 boxes
L12011	LUNA™ Reusable Slide	1 unit
L12014	LUNA™ Reusable Slide Coverslips	10 unit
L72040	Cell Counter Validation Slide-BF	1 unit
L72030	Cell Counter Validation Slide-FL	1 unit
F73101	LUNA-FX™ Calibration Beads Kit	2 x 0.5 mL
T13001	Trypan Blue Stain, 0.4%	2 X 1 mL
T13011	Trypan Blue Stain, 0.4%, Sterile-filtered	2 X 1 mL
L13002	Erythrosin B Stain	2 x 1 mL
F23001	Acridine Orange/Propidium Iodide Stain	2 x 0.5 mL
F23011	Acridine Orange/Propidium Iodide Stain, Sterile-filtered	2 x 0.5 mL
L71001	CountWire™ Basic (1 CountWire™ Data Storage + 1 CountWire™ Verification Key)	1 set
L71002	CountWire™ Verification Key (additional)	1 unit
L74003	LUNA-FX7™ IQ/OQ Protocol	1 copy
P17001	Thermal Printer for LUNA-FX™	1 unit
P17002	Thermal Printer Refills, Paper and Ribbon for LUNA-FX™	2 x 5 rolls

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LUNA-FX7™ Automated Cell Counter

WHAT THE LUNA-FX7™ DOES BEST

THE SOFTWARE THAT CHANGES EVERYTHING.

THE END TO THE ACCURACY DEBATE

EASY, FLEXIBLE AND ROBUST

UNIQUE BIOPROCESS MODE

TRUST YOUR DATA

THROUGHPUT, CONTROL AND EFFICIENCY

COUNTWIRE™ - 21 CFR PART 11 COMPLIANCE

APPLICATION E-BOOK: Ultimate guide to automated cell counting

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Testimonials

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WHERE THE LUNA-FX7™ HAS BEEN CITED

Citations

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Introducing the LUNA-FX7™ Automated Cell Counter

CAR-T Cell Production and Cell Counting with the LUNA-FX7™

Cell DB

ORDERING INFORMATION