Fluorescence Cell Counter
The LUNA-FL™ can count in both brightfield and fluorescence mode, making
it our most versatile counter to date.
Advanced imaging software and GFP and RFP fluorescence optics sensitively
detect fluorescence stained cells.
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
| Cat # | Product | Qty | MSDS | Product Insert | ||||||
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| B13101 | LUNA™ Standard Beads | 2 x 1 mL |
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| T13001 | Trypan Blue Stain, 0.4% | 2 x 1 mL |
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| L13002 | Erythrosin B Stain | 2 x 1 mL |
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| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
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| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
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Children's National Medical Center
The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.
Roger Williams Medical Center
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!
Weill Cornell Medicine / New York-Presbyterian Hospital
The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
Medical University of South Carolina
Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
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CD19-directed CAR T-cell (CTL019) Product Viability and Clinical Outcomes in Non-Hodgkin Lymphomas and B-cell acute lymphoblastic leukemia.
2018. Chong EA, Levine BL, Grupp SA, Davis M, Siegel DL, Maude SL, Gladney WL, Frey NV, Porter DL, June CH, Schuster SJ. Blood 132 (Suppl 1): 197.
Zooming in on cryopreservation of hiPSCs and neural derivatives: A dual-center study using adherent vitrification.
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Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival.
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Development of new 3D human ex vivo models to study sebaceous gland lipid metabolism and modulations.
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Surface modification of macrophages with nucleic acid aptamers for enhancing the immune response against tumor cells.
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Association of the human bocavirus with tonsil squamous cell carcinomas.
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Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow.
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Effect of the bowl structure in an automated cell-isolation device on stromal vascular fraction's isolation yield.
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A single-cell transcriptome atlas of the aging Drosophila brain.
2018. Davie K, Janssens J, Koldere D, De Waegeneer M, Pech U, Kreft Ł, Aibar S, Makhzami S, Christiaens V, Bravo González-Blas C, Poovathingal S, Hulselmans G, Spanier KI, Moerman T, Vanspauwen B, Geurs S, Voet T, Lammertyn J, Thienpont B, Liu S, Konstantinides N, Fiers M, Verstreken P, Aerts S. Cell 174(4): 982-998.
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
2018. Reily C, Rizk DV, Julian BA, Novak J. Biotechniques 65(2): 71-77.
Hair cell mechanotransduction regulates spontaneous activity and spiral ganglion subtype specification in the auditory system.
2018. Sun S, Babola T, Pregernig G, So KS, Nguyen M, Su SM, Palermo AT, Bergles DE, Burns JC, Müller U. Cell 174(5): 1247-1263.
HIV-1 targets L-selectin for adhesion and induces its shedding for viral release.
2018. Kononchik J, Ireland J, Zou Z, Segura J, Holzapfel G, Chastain A, Wang R, Spencer M, He B, Stutzman N, Kano D, Arthos J, Fischer E, Chun TW, Moir S, Sun P. Nature Communications 9(1): 2825.
Strain-specific contribution of eukaryotic elongation factor 1 gamma to the translation of Influenza A virus proteins.
2018. Sammaibashi S, Yamayoshi S, Kawaoka Y. Frontiers in Microbiology 9: 1446.
Transdermal, but not oral mucosa exposure, causes sublethal leptospirosis in mice.
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FGFR signaling regulates resistance of head and neck cancer stem cells to cisplatin.
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Canine mesenchymal stem cells treated with TNF-α and IFN-γ enhance anti-inflammatory effects through the COX-2/PGE2 pathway.
2018. Yang HM, Song WJ, Li Q, Kim SY, Kim HJ, Ryu MO, Ahn JO, Youn HY. Research in Veterinary Science 119: 19-26.
Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration.
2018. Nguyen JT, Ray C, Fox AL, Mendonça DB, Kim JK, Krebsbach PH. Science Advances 4(5): eaao5838.
S1P₄ regulates passive systemic anaphylaxis in mice but is dispensable for canonical IgE-mediated responses in mast cells.
2018. Kulinski JM, Proia RL, Larson EM, Metcalfe DD, Olivera A. International Journal of Molecular Sciences 19(5): 1279.
Mechanical tension drives elongational growth of the embryonic gut.
2018. Chevalier NR, de Witte TM, Cornelissen AJM, Dufour S, Proux-Gillardeaux V, Asnacios A. Scientific Reports 8: 5995.
Dual mechanism of type VII collagen transfer by bone marrow mesenchymal stem cell extracellular vesicles to recessive dystrophic epidermolysis bullosa fibroblasts.
2018. McBride JD, Rodriguez-Menocal L, Candanedo A, Guzman W, Garcia-Contreras M, Badiavas EV. Biochimie 155: 50-58.
Pan-cancer genome and transcriptome analyses of 1,699 paediatric leukaemias and solid tumours.
2018. Ma X, Liu Y, Liu Y, Alexandrov LB, Edmonson MN, Gawad C, Zhou X, Li Y, Rusch MC, Easton J, Huether R, Gonzalez-Pena V, Wilkinson MR, Hermida LC, Davis S, Sioson E, Pounds S, Cao X, Ries RE, Wang Z, Chen X, Dong L, Diskin SJ, Smith MA, Guidry Auvil JM, Meltzer PS, Lau CC,, Perlman EJ, Maris JM, Meshinchi S, Hunger SP, Gerhard DS, Zhang J. Nature 555(7696): 371-376.
B cell receptor and CD40 signaling are rewired for synergistic induction of the c-Myc transcription factor in germinal center B cells.
2018. Luo W, Weisel F, Shlomchik MJ. Immunity 48(2): 313-326.
Improving the quality of a recombinant rabbit monoclonal antibody against PLXDC2 by optimizing transient expression conditions and purification method.
2018. Shimizu H, Nakagawa M, Todaka N, Imaizumi K, Kurosawa Y, Maruyama T, Okumura CJ, Shibata T, Tanaka Y, Sato Y, Ono Y, Akuta T. Protein Expression and Purification 146: 27-33. Oncotarget 8(65): 109358-109369.
Venous endothelium reactivity to Angiotensin II: A study in primary endothelial cultures of rat vena cava and portal vein.
2018. Trindade MR, Assunção HCR, Torres TC, Bertolino JS, Fernandes L. Experimental Cell Research 362(1): 188-194.
Enhancement of recombinant antibody expression level by growth controlled medium.
2018. Phuc LTM, Sasaki T, Shimizu H, Huyen NTM, Thuy NTT, Huan LQ, Taniguchi A. Open Biotechnology Journal 12: 78-85.
Dysregulated connexin 43 in HER2-positive drug resistant breast cancer cells enhances proliferation and migration.
2017. Yeh ES, Williams CJ, Williams CB, Bonilla IV, Klauber-DeMore N, Phillips SL. Oncotarget 8(65): 109358-109369.
Lovastatin induced Kruppel like factor 2 (KLF2), Kruppel like factor 6 (KLF6) and Ras homolog family member B (RHOB) genes and preferentially led to viability reduction of Cisplatin-resistant cells.
2017. Koi C, Izumi H, Kurita T, Nguyen TT, Murakami M, Yoshiura Y2, Hachisuga T, Morimoto Y. Oncotarget 8(63): 106429-106442.
Genome-wide segregation of single nucleotide and structural variants into single cancer cells.
2017. Easton J, Gonzalez-Pena V, Yergeau D, Ma X, Gawad C. BMC Genomics 18(1): 906.
Molecular architecture underlying fluid absorption by the developing inner ear.
2017. Honda K, Kim SH, Kelly MC, Burns JC, Constance L, Li X, Zhou F, Hoa M, Kelley MW, Wangemann P, Morell RJ, Griffith AJ. eLife 6: e26851.
Human induced pluripotent stem cell-derived cardiomyocyte encapsulating bioactive hydrogels improve rat heart function post myocardial infarction.
2017. Chow A, Stuckey DJ, Kidher E, Rocco M, Jabbour RJ, Mansfield CA, Darzi A, Harding SE, Stevens MM, Athanasiou T. Stem Cell Reports 9(5): 1415-1422.
Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury.
2017. Lee SH, Kim Y, Rhew D, Kim A, Jo KR, Yoon Y, Choi KU, Jung T, Kim WH, Kweon OK. Journal of Veterinary Science 18(3): 377-386.
Immunization with recombinant TcdB-encapsulated nanocomplex induces protection against Clostridium difficile challenge in a mouse model.
2017. Liu YW, Chen YH, Chen JW, Tsai PJ, Huang IH. Frontiers in Microbiology: 8:1411.
Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation.
2017. Mizuno M, Katano H, Otabe K, Komori K, Kohno Y, Fujii S, Ozeki N, Horie M, Tsuji K, Koga H, Muneta T, Sekiya I. Stem Cell Research & Therapy 8: 144.
Cell cycle synchronization and analysis of apoptosis‐related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna).
2017. Veraguas D, Gallegos PF, Castro FO, Rodriguez-Alvarez L. Reproduction in Domestic Animals 52(5): 881-889.
Morusin induces apoptosis by regulating expression of Bax and Survivin in human breast cancer cells.
2017. Kang S, Kim EO, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Oncology Letters 13(6): 4558-4562.
MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2.
2017. Werner TV, Hart M, Nickels R, Kim YJ, Menger MD, Bohle RM, Keller A, Ludwig N, Meese E. Aging 9(3): 932-954.
Self-organising aggregates of zebrafish retinal cells for investigating mechanisms of neural lamination.
2017. Eldred MK, Charlton-Perkins M, Muresan L, Harris WA. Development 144(6): 1097-1106.
Functional maturation of human stem cell-derived neurons in long-term cultures.
2017. Lam RS, Töpfer FM, Wood PG, Busskamp V, Bamberg E. PLoS One 12(1): e0169506.
Mycoplasma bovis escapes bovine neutrophil extracellular traps.
2016. Gondaira S, Higuchi H, Nishi K, Iwano H, Nagahata H. Veterinary Microbiology 199: 68-73.
Impact of local injection of brain-derived neurotrophic factor-expressing mesenchymal stromal cells (MSCs) combined with intravenous MSC delivery in a canine model of chronic spinal cord injury.
2016. Lee SH, Kim Y, Rhew D, Kim A, Jo KR, Yoon Y, Choi KU, Jung T, Kim WH, Kweon OK. Cytotherapy 19(1): 75-87.
Tissue transglutaminase activates cancer-associated fibroblasts and contributes to gemcitabine resistance in pancreatic cancer.
2016. Lee J, Yakubov B, Ivan C, Jones DR, Caperell-Grant A, Fishel M, Cardenas H, Matei D. Neoplasia 18(11): 689-698.
An enclosed rotating floating photobioreactor (RFP) powered by flowing water for mass cultivation of photosynthetic microalgae.
2016. Huang JJ, Bunjamin G, Teo ES, Ng DB, Lee YK. Biotechnology for Biofuels 9: 218.
AEG-1 promotes mesenchymal transition through the activation of Rho GTPases in human glioblastoma cells.
2016. Park SY, Choi M, Park D, Jeong M, Ahn KS, Lee J, Fisher PB, Yun M, Lee SG. Oncology Reports 36(5): 2641-2646.
Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing.
2016. Gehwolf R, Wagner A, Lehner C, Bradshaw AD, Scharler C, Niestrawska JA, Holzapfel GA, Bauer HC, Tempfer H, Traweger A. Scientific Reports 6: 32635.
Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model.
2016. Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. The Journal of Pharmacology and Experimental Therapeutics 358(3): 457-63.
Human adipose stromal cells increase survival and mesenteric perfusion following intestinal ischemia and reperfusion injury.
2016. Jensen AR, Doster DL, Hunsberger EB, Manning MM, Stokes SM, Barwinska D, March KL, Yoder MC, Markel TA. Shock 46(1): 75-82.
In vitro endothelialization test of biomaterials using immortalized endothelial cells.
2016. Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. PloS One 11: e0158289.
High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis.
2016. Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. Scientific Reports 6: 27301.
Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site.
2016. Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Biomaterials 101: 310-320.
High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses.
2016. Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. The Journal of Veterinary Medical Science 78(9): 1405-1411.
Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors.
2016. Ramirez K, Sheridan JF. Brain, Behavior, and Immunity 57:293-303.
Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury.
2016. Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. The Journal of Surgical Research 204(2): 361-370.
Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity.
2016. Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Nature Medicine 22(5): 547-556.
Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction.
2016. Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. International Journal of Oncology 48(5): 2205-12.
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Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform.
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Brochure
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Quick Start Guide
Quick Start Guide
Product literature
Product literature
Product literature
Product literature
Product literature
Product literature
Experimental condition
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
Experimental condition
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Experimental condition
Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Mouse splenocytes were collected directly from spleen tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Mouse thymocytes were collected directly from the thymus tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Yeast (Saccharomyces Cerevisiae) cells were cultured in the YPD medium supplemented with several antibiotics overnight. The yeast cells were artificially killed by heating and mixed with healthy cells (50:50). Mixed cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
A high density 3 micron-sized beads were counted with the LUNA-FL to verifiy the performance of the instrument. Beads were counted at the bright field cell counting mode of LUNA-FL. Orange circles represent the automatically-classified objects by LUNA-FL.
Experimental condition
The number and the viability of yeast cells were measured in a bottle of a Korean rice wine. The wine was diluted at 1:100 with PBS, and the cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter
| Cat # | Product | Qty |
|---|---|---|
| L20001 | LUNA-FL™ Automated Fluorescence Cell Counter | 1 unit |
| L12005 | PhotonSlide™, 50 Slides | 1 box |
| L12006 | PhotonSlide™, 500 Slides | 10 boxes |
| L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
| L12001 | LUNA™ Cell Counting Slides, 50 Slides | 1 box |
| L12002 | LUNA™ Cell Counting Slides, 500 Slides | 10 boxes |
| L12003 | LUNA™ Cell Counting Slides, 1000 Slides | 20 boxes |
| L12011 | LUNA™ Reusable Slide | 1 unit |
| L12012 | LUNA™ Reusable Slides | 2 units |
| L12014 | LUNA™ Reusable Slide Coverslips | 10 units |
| T13001 | Trypan Blue Stain, 0.4% | 2 X 1 mL |
| L13002 | Erythrosin B Stain | 2 x 1 mL |
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
| F23002 | Acridine Orange Stain | 2 x 0.5 mL | <
| F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
| F23202 | Yeast Viability Kit 1 - Propidium Iodide Stain for Yeast (1 x 0.5 mL) - Fluorescein Diacetate Stain (1 x 0.5 mL) - Cell Dilution Buffer (1 x 20 mL) - Fluorescence Signal Enhancer 1 (1 x 0.5 mL) |
1 kit |
| F23212 | Cell Dilution Buffer | 5 x 20 mL |
| F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
| B13101 | LUNA™ Standard Beads | 2 x 1 mL |
| F24003 | LUNA-FL™ IQ/OQ Protocol | 1 copy |
| P10001 | LUNA™ Printer | 1 unit |
| P10002 | LUNA™ Printer Paper - thermal, 700 prints | 3 x 2 rolls |
