Fluorescence Cell Counter
The LUNA-FL™ can count in both brightfield and fluorescence mode, making
it our most versatile counter to date.
Advanced imaging software and GFP and RFP fluorescence optics sensitively
detect fluorescence stained cells.
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
| Cat # | Product | Qty | MSDS | Product Insert | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| T13001 | Trypan Blue Stain, 0.4% | 2 x 1 mL |
|
|||||||
| L13002 | Erythrosin B Stain | 2 x 1 mL |
|
|||||||
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
|
|||||||
| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
|
|||||||
Weill Cornell Medicine / NewYork-Presbyterian Hospital
The LUNA-FL Dual Fluorescence Cell Counter consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
Medical University of South Carolina
Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images.
Hong D, Lee G, Jung NC, Jeon M.
Biological procedures online. 15(1):13.(2013)
Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors.
Ramirez K, Sheridan JF.
Brain, behavior, and immunity. 57:293-303 (2016)
High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses.
Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K.
The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1;78(9):1405-1411. (2016)
Minicirclemicroporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site.
Mun JY, Shin KK, Kwon O, Lim YT, Oh DB.
Biomaterials. 101:310-320 (2016)
Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model.
Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S.
The Journal of pharmacology and experimental therapeutics. 358(3):457-63. (2016)
Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts.
Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C.
Journal of medical virology. 88:1408-1416(2016)
Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury.
Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA.
The Journal of surgical research. 204:361-370 (2016)
In vitro endothelialization test of biomaterials using immortalized endothelial cells.
Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S.
PloS one. 11:e0158289 (2016)
High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis.
Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E.
Scientific reports. 6:27301 (2016)
Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction.
Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M.
International journal of oncology. 48(5):2205-12.
Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity.
Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J.
Nature medicine. 22:547-556 (2016)
Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform.
Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M.
Nucleic acids research. 44:e88 (2016)
Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone.
Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W.
Stem Cells 34(4):888-901 (2016)
The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles.
Nagai K, Yanagawa Y, Katagiri S, Nagano M.
Biomedical research. 37:63-71 (2016)
Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model.
Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T.
J BioactCompatPolym 2016; doi: 10.1177/0883911516635841
Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells.
Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG.
Journal of natural products. 79:317-323 (2016)
Application of a non-hazardous vital dye for cell counting with automated cell counters.
Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW.
Analytical biochemistry,1;492:8-12(2016)
Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles.
Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M.
Reproductive biology and endocrinology : RB&E. 15;14:3 (2016)
Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells.
Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H.
Plasma Med 2016; 10.1615/PlasmaMed.2016016219
GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations.
Ramirez K, Niraula A, Sheridan JF.
Brain, behavior, and immunity 51:154-68 (2016)
Mouse model for sublethal Leptospira interrogans infection.
Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M.
Infection and immunity. 83(12):4693-700.(2015)
Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury.
Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. J
The Journal of surgical research. 199(1):56-66.2015;
Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6.
Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I.
International journal of oncology. 47(1):122-32 (2015)
Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor.
Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG.
Molecules and cells. 38(6):548-61. (2015)
Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance.
Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF.
Brain, behavior, and immunity. 46:212-20.
Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones.
Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T.
Stem cells international. 2015:165867 (2015)
β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells.
Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I.
Experimental & molecular medicine. 47:e143. (2015)
Quick Start Guide
Quick Start Guide
Brochure
Product literature
Product literature
Product literature
Product literature
Product literature
Brochure
Product literature
Experimental condition
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
Experimental condition
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Experimental condition
Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Mouse splenocytes were collected directly from spleen tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Mouse thymocytes were collected directly from the thymus tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Yeast (Saccharomyces Cerevisiae) cells were cultured in the YPD medium supplemented with several antibiotics overnight. The yeast cells were artificially killed by heating and mixed with healthy cells (50:50). Mixed cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
A high density 3 micron-sized beads were counted with the LUNA-FL to verifiy the performance of the instrument. Beads were counted at the bright field cell counting mode of LUNA-FL. Orange circles represent the automatically-classified objects by LUNA-FL.
Experimental condition
The number and the viability of yeast cells were measured in a bottle of a Korean rice wine. The wine was diluted at 1:100 with PBS, and the cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter
| Cat # | Product | Qty |
|---|---|---|
| L20001 | LUNA-FL™ Automated Fluorescence Cell Counter | 1 unit |
| L12005 | PhotonSlide™, 50 Slides | 1 box |
| L12006 | PhotonSlide™, 500 Slides | 10 boxes |
| L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
| L12001 | LUNA™ Cell Counting Slides, 50 Slides | 1 box |
| L12002 | LUNA™ Cell Counting Slides, 500 Slides | 10 boxes |
| L12003 | LUNA™ Cell Counting Slides, 1000 Slides | 20 boxes |
| T13001 | Trypan Blue Stain, 0.4% | 2 X 1 mL |
| L13002 | Erythrosin B Stain | 2 x 1 mL |
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
| F23003 | Yeast Viability Kit 1 - Propidium Iodide Stain for Yeast (1 x 0.5 mL) - Fluorescein Diacetate Stain (1 x 0.5 mL) - Cell Dilution Buffer (1 x 20 mL) - Fluorescence Signal Enhancer 1 (1 x 0.5 mL) |
1 kit |
| F23212 | Cell Dilution Buffer | 5 x 20 mL |
| F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
| B13101 | LUNA™ Standard Beads | 2 x 1 mL |
| P10001 | LUNA™ Printer | 1 unit |
| P10002 | LUNA™ Printer Paper - thermal, 700 prints | 3 x 2 rolls |
