• LUNA-FL Dual Fluorescence Cell Counter
  • Fluorescence Cell Counter

    LUNA-FL™ Dual Fluorescence Cell Counter

    Equipped with dual fluorescence and brightfield optics, the LUNA-FL™ is a powerhouse. The LUNA-FL™ can assess cell counts, viability, and GFP transfection efficiency without being limited by cell type or size.
    download software
With four counting modes, the LUNA-FL™ is our powerhouse. Equipped with brightfield and dual fluorescence optics that allows the sensitive detection of most cell types excluding bacteria. The LUNA-FL™ can distinguish primary cells such as PBMCs, splenocytes, neutrophils, and stem cells from undesirable debris for accurate cell count and viability results. Brightfield cell counting, fluorescence cell counting, yeast cell counting, and GFP transfection assays are all in the LUNA-FL™ wheelhouse. This versatile little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

WHAT THE LUNA-FL™ DOES BEST

  • LUNA-FL Dual Fluorescence Cell Counter two counters in one
    TWO COUNTERS IN ONE

    The LUNA-FL™ can count in both brightfield and fluorescence mode, making
    it our most versatile counter to date.

  • LUNA-FL Dual Fluorescence Cell Counter compatible with most cells
    COMPATIBLE WITH MOST CELLS

    Advanced imaging software and GFP and RFP fluorescence optics sensitively
    detect fluorescence stained cells.

  • LUNA-FL Dual Fluorescence Cell Counter gfp transfection analysis
    GFP TRANSFECTION ANALYSIS

    High resolution images are captured and analyzed to detect GFP-positive and -negative cells.

  • LUNA-FL Dual Fluorescence Cell Counter maximum 30 seconds
    MAXIMUM 30 SECONDS

    Fluorescence: 30 s = 3 images captured & analyzed;
    Brightfield: 10 s = 1 image captured & analyzed

THE SOFTWARE THAT CHANGES EVERYTHING.

FOUR COUNTING MODES CUSTOMIZE & SAVE PROTOCOLS USE THE DILUTION CALCULATOR GATE CELLS ON HISTOGRAMS

 

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Logos Biosystems LUNA Reusable Slide
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images. Hong D, Lee G, Jung NC, Jeon M. Biological procedures online. 15(1):13.(2013) Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors. Ramirez K, Sheridan JF. Brain, behavior, and immunity. 57:293-303 (2016) High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses. Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 1;78(9):1405-1411. (2016) Minicirclemicroporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site. Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Biomaterials. 101:310-320 (2016) Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model. Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. The Journal of pharmacology and experimental therapeutics. 358(3):457-63. (2016) Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts. Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C. Journal of medical virology. 88:1408-1416(2016) Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury. Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. The Journal of surgical research. 204:361-370 (2016) In vitro endothelialization test of biomaterials using immortalized endothelial cells. Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. PloS one. 11:e0158289 (2016) High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis. Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. Scientific reports. 6:27301 (2016) Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction. Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. International journal of oncology. 48(5):2205-12. Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity. Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Nature medicine. 22:547-556 (2016) Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform. Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M. Nucleic acids research. 44:e88 (2016) Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone. Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W. Stem Cells 34(4):888-901 (2016) The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles. Nagai K, Yanagawa Y, Katagiri S, Nagano M. Biomedical research. 37:63-71 (2016) Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model. Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T. J BioactCompatPolym 2016; doi: 10.1177/0883911516635841 Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells. Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Journal of natural products. 79:317-323 (2016) Application of a non-hazardous vital dye for cell counting with automated cell counters. Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical biochemistry,1;492:8-12(2016) Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles. Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M. Reproductive biology and endocrinology : RB&E. 15;14:3 (2016) Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells. Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H. Plasma Med 2016; 10.1615/PlasmaMed.2016016219 GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations. Ramirez K, Niraula A, Sheridan JF. Brain, behavior, and immunity 51:154-68 (2016) Mouse model for sublethal Leptospira interrogans infection. Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M. Infection and immunity. 83(12):4693-700.(2015) Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury. Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. J The Journal of surgical research. 199(1):56-66.2015; Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6. Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I. International journal of oncology. 47(1):122-32 (2015) Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor. Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG. Molecules and cells. 38(6):548-61. (2015) Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance. Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Brain, behavior, and immunity. 46:212-20. Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones. Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T. Stem cells international. 2015:165867 (2015) β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. Experimental & molecular medicine. 47:e143. (2015)
LUNA-FL™: GFP Transfection Assay Quick Start Guide LUNA-FL™ Quick Start Guide LUNA-FL™ Brochure Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images Product literature Application of a non-hazardous vital dye for cell counting with automated cell counters GFP expression analysis using the LUNA-FL™ Fast cell counting and viability measurement of yeast cells with the LUNA-FL™ Automated fluorescence cell counting Logos Biosystems Products Brochure LUNA-FL™ software 3.0.1 Software Autoexposure mode update Notice
Compatible Reagents
    Cat # Product Qty MSDS Product Insert
    T13001 Trypan Blue Stain, 0.4%

    2 x 1 mL

    FileAction
    [T13001] Trypan Blue Stain_0.4%.pdfDownload 
    T13001 Trypan Blue Stain, 0.4% VL1801-01.pdfDownload 
    L13002 Erythrosin B Stain

    2 x 1 mL

    FileAction
    [L13002] Erythrosin B Stain.pdfDownload 
    L13002 Erythrosin B Stain VL1801-01.pdfDownload 
    F23001 Acridine Orange/Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23001] Acridine Orange Propidium Iodide (AOPI) Stain.pdfDownload 
    F23001 Acridine Orange,Propidium Iodide Stain VL1801-01.pdfDownload 
    F23002 Acridine Orange Stain

    2 x 0.5 mL

    FileAction
    [F23002] Acridine Orange (AO) Stain.pdfDownload 
    F23002 Acridine Orange Stain VL1801-01.pdfDownload 
    F23003 Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23003] Propidium Iodide (PI) Stain.pdfDownload 
    F23003 Propidium Iodide Stain VL1801-01.pdfDownload 
    F23202 Yeast Viability Kit 1

    1 kit

    FileAction
    [F23202] Yeast Viability Kit 1.zipDownload 
    F23202 Yeast Viability Kit 1 VL1801-01.pdfDownload 
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    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Gelina Sani

      Children's National Medical Center

      The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.

    • Mikayla Lopes

      Roger Williams Medical Center

      The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!

    • Regina Wulff, MS

      Weill Cornell Medicine / New York-Presbyterian Hospital

      The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.

    • Danh Tran

      Medical University of South Carolina

      Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.

    • Woong Sun, PhD

      Korea University Medical School

      I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.

    • May Malicdan, MD PhD

      NIH, NHGRI

      It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.

    • Joanna Ireland, PhD

      NIH, NIAID

      It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.

    + SHOW OUR CUSTOMERS

    WHERE THE LUNA-FL™ HAS BEEN CITED

    Citations
    • Mitigation of T-cell dependent immunogenicity by reengineering factor VIIa analogue.
      2019. Jankowski W, McGill J, Lagassé HAD, Surov S, Bembridge G, Bunce C, Cloake E, Fogg MH, Jankowska KI, Khan A, Marcotrigiano J, Ovanesov MV, Sauna ZE. Blood Advances 3(17): 2668-2678.

    • A comparison of ovine facial and limb muscle as a primary cell source for engineered skeletal muscle.
      2019. Rodriguez BL, Nguyen MH, Armstrong RE, Vega-Soto EE, Polkowski PM, Larkin LM. Tissue Engineering Part A doi.org/10.1089/ten.TEA.2019.0087.

    • Roles of interleukin-11 during acute bacterial pneumonia.
      2019. Traber KE, Dimbo EL, Symer EM, Korkmaz FT, Jones MR, Mizgerd JP, Quinton LJ. PLoS One 14(8): e0221029.

    • DLC1 deficiency and YAP signaling drive endothelial cell contact inhibition of growth and tumorigenesis.
      2019. Ritchey L, Ha T, Otsuka A, Kabashima K, Wang D, Wang Y, Lowy DR, Tosato G. Oncogene 10.1038/s41388-019-0944-x.

    • Diffusion-weighted MRI and 18F-FDG PET correlation with immunity in early radiotherapy response in BNL hepatocellular carcinoma mouse model: timeline validation
      2019. Chung YH, Yu CF, Chiu SC, Chiu H, Hsu ST, Wu CR, Yang CL, Hong JH, Yen TC, Chen FH. European Journal of Nuclear Medicine and Molecular Imaging: 1-12.

    • A microwell array platform to print and measure biomolecules produced by single cells
      2019. Abali F, Broekmaat J, Tibbe A, Schasfoort RBM, Zeunea L, Terstappen LWMM. Lab on a Chip 19: 1850-1859.

    • Thrombin preconditioning of extracellular vesicles derived from mesenchymal stem cells accelerates cutaneous wound healing by boosting their biogenesis and enriching cargo content.
      2019. Sung DK, Chang YS, Sung SI, Ahn SY, Park WS. Journal of Clinical Medicine 8(4): 533.

    • Homotypic and heterotypic trans-assembly of human Rab-family small GTPases in reconstituted membrane tethering.
      2019. Segawa K, Tamura N, Mima J. Journal of Biological Chemistry 294: 7722-7739.

    • Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells.
      2019. Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Mizuguchi H, Sato Y. Scientific Reports 9(1): 3630.

    • Chimeric antigen receptor costimulation domains modulate human regulatory T cell function.
      2019. Boroughs AC, Larson RC, Choi BD, Bouffard AA, Riley LS, Schiferle E, Kulkarni AS, Cetrulo CL, Ting D, Blazar BR, Demehri S, Maus MV. JCI Insight 4(8): e126194.

    • Exploiting evolutionary herding to control drug resistance in cancer.
      2019. Acar A, Nichol D, Fernandez-Mateos J, Cresswell GD, Barozzi I, Hong SP, Spiteri I, Stubbs M, Burke R, Stewart A, Vlachogiannis G, Maley CC, Magnani L, Valeri N, Banerji U, Sottoriva A. bioRxiv 566950.

    • SOX-6, 9-transfected adipose stem cells to treat surgically-induced osteoarthritis in goats.
      2019. Ko JY, Lee J, Lee J, Ryu YH, Im GI. Tissue Engineering Part A.

    • Initial assessment of variability of responses to toxicants in donor-specific endothelial colony forming cells.
      2018. Filonov D, Tice R, Luo R, Grotegut C, Van Kanegan MJ, Ludlow JW, Il'yasova D, Kinev A. Frontiers in Public Health 6: 369.

    • Predicted glycosyltransferases promote development and prevent spurious cell clumping in the choanoflagellate S. rosetta.
      2018. Wetzel LA, Levin TC, Hulett RE, Chan D, King GA, Aldayafleh R, Booth DS, Sigg MA, King N. ELife 7: e41482.

    • Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy.
      2018. Hong SP, Chan TE, Lombardo Y, Corleone G, Rotmensz N, Pruneri G, McEwen KR, Coombes RC, Ali S, Barozzil I, Magnani L. Nature Communications – under consideration.

    • CD19-directed CAR T-cell (CTL019) Product Viability and Clinical Outcomes in Non-Hodgkin Lymphomas and B-cell acute lymphoblastic leukemia.
      2018. Chong EA, Levine BL, Grupp SA, Davis M, Siegel DL, Maude SL, Gladney WL, Frey NV, Porter DL, June CH, Schuster SJ. Blood 132 (Suppl 1): 197.

    • Zooming in on cryopreservation of hiPSCs and neural derivatives: A dual-center study using adherent vitrification.
      2018. Kaindl J, Meiser I, Majer J, Sommer A, Krach F, Katsen-Globa A, Winkler J, Zimmermann H, Neubauer JC, Winner B. Stem Cells Translational Medicine 8(3): 247-259.

    • Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival.
      2018. Wang F, Bonam SR, Schall N, Kuhn L, Hammann P, Chaloin O, Madinier JB, Briand JP, Page N, Muller S. Scientific Reports 8(1): 16820.

    • Development of new 3D human ex vivo models to study sebaceous gland lipid metabolism and modulations.
      2018. de Bengy AF, Forraz N, Danoux L, Berthelemy N, Cadau S, Degoul O, Andre V, Pain S, McGuckin C. Cell Proliferation 52(1): e12524.

    • Surface modification of macrophages with nucleic acid aptamers for enhancing the immune response against tumor cells.
      2018. Sugimoto S, Iwasaki Y. Bioconjugate Chemistry 29(12): 4160-4167.

    • Association of the human bocavirus with tonsil squamous cell carcinomas.
      2018. Höpken M, Förster I, Maune S, Brockmann M, Schildgen O, Schildgen V. Frontiers in Microbiology 9: 2450.

    • Pregn-5-en-3β-ol and androst-5-en-3β-ol dicarboxylic acid esters as potential therapeutics for NMDA hypofunction: In vitro safety assessment and plasma stability.
      2018. Matousova M, Soucek R, Tloustova E, Slavikova B, Chodounska H, Mertlikova-Kaiserova H, Kudova E. Steroids.

    • Choanoflagellate transfection illuminates their cell biology and the ancestry of animal septins.
      2018. Booth DS, Szmidt-Middleton H, King N. Molecular Biology of the Cell 29(25): 3026-3038.

    • Glycosyltransferases promote development and prevent promiscuous cell aggregation in the choanoflagellate S. rosetta.
      2018. Wetzel LA, Levin TC, Hulett RE, Chan D, King GA, Aldayafleh R, Booth DS, Sigg MA, King N. bioRxiv 384453.

    • Self-maintaining gut macrophages are essential for intestinal homeostasis.
      2018. De Schepper S, Verheijden S, Aguilera-Lizarraga J, Viola MF, Boesmans W, Stakenborg N, Voytyuk I, Schmidt I, Boeckx B, Dierckx de Casterlé I, Baekelandt V, Gonzalez Dominguez E, Mack M, Depoortere I, De Strooper B, Sprangers B, Himmelreich U, Soenen S, Guilliams M, Vanden Berghe P, Jones E, Lambrechts D, Boeckxstaens G. Cell 175(2): 400-415.

    • Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow.
      2018. Sasaki A, Mizuno M, Ozeki N, Katano H, Otabe K, Tsuji K, Koga H, Mochizuki M, Sekiya I. PLoS One 13(8): e0202922.

    • Effect of the bowl structure in an automated cell-isolation device on stromal vascular fraction's isolation yield.
      2018. Hahn HM, Jeong KS, Yoo BY, Park JH, Jung HJ, Lee IJ. Journal of Medical Devices 12(4): 044501.

    • A single-cell transcriptome atlas of the aging Drosophila brain.
      2018. Davie K, Janssens J, Koldere D, De Waegeneer M, Pech U, Kreft Ł, Aibar S, Makhzami S, Christiaens V, Bravo González-Blas C, Poovathingal S, Hulselmans G, Spanier KI, Moerman T, Vanspauwen B, Geurs S, Voet T, Lammertyn J, Thienpont B, Liu S, Konstantinides N, Fiers M, Verstreken P, Aerts S. Cell 174(4): 982-998.

    • Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
      2018. Reily C, Rizk DV, Julian BA, Novak J. Biotechniques 65(2): 71-77.

    • Hair cell mechanotransduction regulates spontaneous activity and spiral ganglion subtype specification in the auditory system.
      2018. Sun S, Babola T, Pregernig G, So KS, Nguyen M, Su SM, Palermo AT, Bergles DE, Burns JC, Müller U. Cell 174(5): 1247-1263.

    • HIV-1 targets L-selectin for adhesion and induces its shedding for viral release.
      2018. Kononchik J, Ireland J, Zou Z, Segura J, Holzapfel G, Chastain A, Wang R, Spencer M, He B, Stutzman N, Kano D, Arthos J, Fischer E, Chun TW, Moir S, Sun P. Nature Communications 9(1): 2825.

    • Strain-specific contribution of eukaryotic elongation factor 1 gamma to the translation of Influenza A virus proteins.
      2018. Sammaibashi S, Yamayoshi S, Kawaoka Y. Frontiers in Microbiology 9: 1446.

    • Transdermal, but not oral mucosa exposure, causes sublethal leptospirosis in mice.
      2018. Nair N, Gomes-Solecki M. bioRxiv 334219.

    • FGFR signaling regulates resistance of head and neck cancer stem cells to cisplatin.
      2018. McDermott SC, Rodriguez-Ramirez C, McDermott SP, Wicha MS, Nör JE. Oncotarget 9(38): 25148-25165.

    • Canine mesenchymal stem cells treated with TNF-α and IFN-γ enhance anti-inflammatory effects through the COX-2/PGE2 pathway.
      2018. Yang HM, Song WJ, Li Q, Kim SY, Kim HJ, Ryu MO, Ahn JO, Youn HY. Research in Veterinary Science 119: 19-26.

    • Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration.
      2018. Nguyen JT, Ray C, Fox AL, Mendonça DB, Kim JK, Krebsbach PH. Science Advances 4(5): eaao5838.

    • S1P₄ regulates passive systemic anaphylaxis in mice but is dispensable for canonical IgE-mediated responses in mast cells.
      2018. Kulinski JM, Proia RL, Larson EM, Metcalfe DD, Olivera A. International Journal of Molecular Sciences 19(5): 1279.

    • Mechanical tension drives elongational growth of the embryonic gut.
      2018. Chevalier NR, de Witte TM, Cornelissen AJM, Dufour S, Proux-Gillardeaux V, Asnacios A. Scientific Reports 8: 5995.

    • Dual mechanism of type VII collagen transfer by bone marrow mesenchymal stem cell extracellular vesicles to recessive dystrophic epidermolysis bullosa fibroblasts.
      2018. McBride JD, Rodriguez-Menocal L, Candanedo A, Guzman W, Garcia-Contreras M, Badiavas EV. Biochimie 155: 50-58.

    • Pan-cancer genome and transcriptome analyses of 1,699 paediatric leukaemias and solid tumours.
      2018. Ma X, Liu Y, Liu Y, Alexandrov LB, Edmonson MN, Gawad C, Zhou X, Li Y, Rusch MC, Easton J, Huether R, Gonzalez-Pena V, Wilkinson MR, Hermida LC, Davis S, Sioson E, Pounds S, Cao X, Ries RE, Wang Z, Chen X, Dong L, Diskin SJ, Smith MA, Guidry Auvil JM, Meltzer PS, Lau CC,, Perlman EJ, Maris JM, Meshinchi S, Hunger SP, Gerhard DS, Zhang J. Nature 555(7696): 371-376.

    • B cell receptor and CD40 signaling are rewired for synergistic induction of the c-Myc transcription factor in germinal center B cells.
      2018. Luo W, Weisel F, Shlomchik MJ. Immunity 48(2): 313-326.

    • Improving the quality of a recombinant rabbit monoclonal antibody against PLXDC2 by optimizing transient expression conditions and purification method.
      2018. Shimizu H, Nakagawa M, Todaka N, Imaizumi K, Kurosawa Y, Maruyama T, Okumura CJ, Shibata T, Tanaka Y, Sato Y, Ono Y, Akuta T. Protein Expression and Purification 146: 27-33. Oncotarget 8(65): 109358-109369.

    • Venous endothelium reactivity to Angiotensin II: A study in primary endothelial cultures of rat vena cava and portal vein.
      2018. Trindade MR, Assunção HCR, Torres TC, Bertolino JS, Fernandes L. Experimental Cell Research 362(1): 188-194.

    • Enhancement of recombinant antibody expression level by growth controlled medium.
      2018. Phuc LTM, Sasaki T, Shimizu H, Huyen NTM, Thuy NTT, Huan LQ, Taniguchi A. Open Biotechnology Journal 12: 78-85.

    • Dysregulated connexin 43 in HER2-positive drug resistant breast cancer cells enhances proliferation and migration.
      2017. Yeh ES, Williams CJ, Williams CB, Bonilla IV, Klauber-DeMore N, Phillips SL. Oncotarget 8(65): 109358-109369.

    • Lovastatin induced Kruppel like factor 2 (KLF2), Kruppel like factor 6 (KLF6) and Ras homolog family member B (RHOB) genes and preferentially led to viability reduction of Cisplatin-resistant cells.
      2017. Koi C, Izumi H, Kurita T, Nguyen TT, Murakami M, Yoshiura Y2, Hachisuga T, Morimoto Y. Oncotarget 8(63): 106429-106442.

    • Genome-wide segregation of single nucleotide and structural variants into single cancer cells.
      2017. Easton J, Gonzalez-Pena V, Yergeau D, Ma X, Gawad C. BMC Genomics 18(1): 906.

    • Molecular architecture underlying fluid absorption by the developing inner ear.
      2017. Honda K, Kim SH, Kelly MC, Burns JC, Constance L, Li X, Zhou F, Hoa M, Kelley MW, Wangemann P, Morell RJ, Griffith AJ. eLife 6: e26851.

    • Human induced pluripotent stem cell-derived cardiomyocyte encapsulating bioactive hydrogels improve rat heart function post myocardial infarction.
      2017. Chow A, Stuckey DJ, Kidher E, Rocco M, Jabbour RJ, Mansfield CA, Darzi A, Harding SE, Stevens MM, Athanasiou T. Stem Cell Reports 9(5): 1415-1422.

    • Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury.
      2017. Lee SH, Kim Y, Rhew D, Kim A, Jo KR, Yoon Y, Choi KU, Jung T, Kim WH, Kweon OK. Journal of Veterinary Science 18(3): 377-386.

    • Immunization with recombinant TcdB-encapsulated nanocomplex induces protection against Clostridium difficile challenge in a mouse model.
      2017. Liu YW, Chen YH, Chen JW, Tsai PJ, Huang IH. Frontiers in Microbiology: 8:1411.

    • Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation.
      2017. Mizuno M, Katano H, Otabe K, Komori K, Kohno Y, Fujii S, Ozeki N, Horie M, Tsuji K, Koga H, Muneta T, Sekiya I. Stem Cell Research & Therapy 8: 144.

    • Cell cycle synchronization and analysis of apoptosis‐related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna).
      2017. Veraguas D, Gallegos PF, Castro FO, Rodriguez-Alvarez L. Reproduction in Domestic Animals 52(5): 881-889.

    • Morusin induces apoptosis by regulating expression of Bax and Survivin in human breast cancer cells.
      2017. Kang S, Kim EO, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Oncology Letters 13(6): 4558-4562.

    • MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2.
      2017. Werner TV, Hart M, Nickels R, Kim YJ, Menger MD, Bohle RM, Keller A, Ludwig N, Meese E. Aging 9(3): 932-954.

    • Self-organising aggregates of zebrafish retinal cells for investigating mechanisms of neural lamination.
      2017. Eldred MK, Charlton-Perkins M, Muresan L, Harris WA. Development 144(6): 1097-1106.

    • Functional maturation of human stem cell-derived neurons in long-term cultures.
      2017. Lam RS, Töpfer FM, Wood PG, Busskamp V, Bamberg E. PLoS One 12(1): e0169506.

    • Mycoplasma bovis escapes bovine neutrophil extracellular traps.
      2016. Gondaira S, Higuchi H, Nishi K, Iwano H, Nagahata H. Veterinary Microbiology 199: 68-73.

    • Impact of local injection of brain-derived neurotrophic factor-expressing mesenchymal stromal cells (MSCs) combined with intravenous MSC delivery in a canine model of chronic spinal cord injury.
      2016. Lee SH, Kim Y, Rhew D, Kim A, Jo KR, Yoon Y, Choi KU, Jung T, Kim WH, Kweon OK. Cytotherapy 19(1): 75-87.

    • Tissue transglutaminase activates cancer-associated fibroblasts and contributes to gemcitabine resistance in pancreatic cancer.
      2016. Lee J, Yakubov B, Ivan C, Jones DR, Caperell-Grant A, Fishel M, Cardenas H, Matei D. Neoplasia 18(11): 689-698.

    • An enclosed rotating floating photobioreactor (RFP) powered by flowing water for mass cultivation of photosynthetic microalgae.
      2016. Huang JJ, Bunjamin G, Teo ES, Ng DB, Lee YK. Biotechnology for Biofuels 9: 218.

    • AEG-1 promotes mesenchymal transition through the activation of Rho GTPases in human glioblastoma cells.
      2016. Park SY, Choi M, Park D, Jeong M, Ahn KS, Lee J, Fisher PB, Yun M, Lee SG. Oncology Reports 36(5): 2641-2646.

    • Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing.
      2016. Gehwolf R, Wagner A, Lehner C, Bradshaw AD, Scharler C, Niestrawska JA, Holzapfel GA, Bauer HC, Tempfer H, Traweger A. Scientific Reports 6: 32635.

    • Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model.
      2016. Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. The Journal of Pharmacology and Experimental Therapeutics 358(3): 457-63.

    • Human adipose stromal cells increase survival and mesenteric perfusion following intestinal ischemia and reperfusion injury.
      2016. Jensen AR, Doster DL, Hunsberger EB, Manning MM, Stokes SM, Barwinska D, March KL, Yoder MC, Markel TA. Shock 46(1): 75-82.

    • In vitro endothelialization test of biomaterials using immortalized endothelial cells.
      2016. Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. PloS One 11: e0158289.

    • High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis.
      2016. Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. Scientific Reports 6: 27301.

    • Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site.
      2016. Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Biomaterials 101: 310-320.

    • High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses.
      2016. Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. The Journal of Veterinary Medical Science 78(9): 1405-1411.

    • Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors.
      2016. Ramirez K, Sheridan JF. Brain, Behavior, and Immunity 57:293-303.

    • Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury.
      2016. Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. The Journal of Surgical Research 204(2): 361-370.

    • Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity.
      2016. Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Nature Medicine 22(5): 547-556.

    • Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction.
      2016. Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. International Journal of Oncology 48(5): 2205-12.

    • Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model.
      2016. Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T. Journal of Bioactive and Compatible Polymers 31(6): 600-612.

    • Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform.
      2016. Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M. Nucleic Acids Research 44(9): e88.

    • Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts.
      2016. Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C. Journal of Medical Virology 88(8): 1408-1416.

    • Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells.
      2016. Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Journal of Natural Products 79(2): 317-323.

    • The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles.
      2016. Nagai K, Yanagawa Y, Katagiri S, Nagano M. Biomedical Research 37(1): 63-71.

    • Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone.
      2016. Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W. Stem Cells 34(4): 888-901.

    • Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles.
      2016. Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M. Reproductive Biology and Endocrinology 14:3.

    • Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells.
      2016. Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H. Plasma Medicine 5(2–4): 271–281.

    • Application of a non-hazardous vital dye for cell counting with automated cell counters.
      2016. Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical Biochemistry 492: 8-12.

    • Mouse model for sublethal Leptospira interrogans infection.
      2015. Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M. Infection and Immunity 83(12): 4693-700.

    • GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations.
      2016. Ramirez K, Niraula A, Sheridan JF. Brain, Behavior, and Immunity 51:154-68.

    • Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury.
      2015. Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. The Journal of Surgical Research. 199(1): 56-66.

    • Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones.
      2015. Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T. Stem Cells International. 2015: 165867.

    • Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor.
      2015. Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG. Molecules and Cells 38(6): 548-61.

    • Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6.
      2015. Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I. International Journal of Oncology. 47(1): 122-32.

    • β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells.
      2015. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. Experimental & Molecular Medicine. 47: e143.

    • Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance.
      2015. Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Brain, Behavior, and Immunity 46: 212-20.

    • Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images.
      2013. Hong D, Lee G, Jung NC, Jeon M. Biological Procedures Online 15(1): 13.

    SHOW MORE  +

    Resources

    Downloads
    • Icon
      Logos Biosystems Products

      Brochure

      2019-07-16 | 2.14 MB
    • Icon
      LUNA-FL™

      Brochure

      2018-04-18 | 2.1 MB
    • Icon
      LUNA-FL™: GFP Transfection Assay

      Quick Start Guide

      2018-04-16 | 110 KB
    • Icon
      LUNA-FL™

      Quick Start Guide

      2018-04-16 | 284 KB
    • Icon
      Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images

      Product literature

      2018-04-16 | 1.7 MB
    • Icon
      GFP expression analysis using the LUNA-FL™

      Product literature

      2018-04-16 | 403 MB
    • Icon
      Fast cell counting and viability measurement of yeast cells with the LUNA-FL™

      Product literature

      2018-04-16 | 862 KB
    • Icon
      Automated fluorescence cell counting

      Product literature

      2018-04-16 | 476 KB
    • Icon
      Application of a non-hazardous vital dye for cell counting with automated cell counters

      Product literature

      2018-04-13 | 906 KB
    • Icon
      Using the LUNA Reusable Slide for accurate cell counting with automated cell counters

      Product literature

      2018-04-13 | 665.84 KB
    • Icon
      LUNA-FL™ software 3.0.1

      Software

      2018-04-16 | 3.4 MB
    • Icon
      Autoexposure mode update

      Notice

      2018-04-16 | 2.2 MB
      Videos

      How to Use the LUNA™ Reusable Slide

      LUNA-FL™ Demo

      Introducing the LUNA-FL™

      Cell DB
        • LUNA-FL™ Dual Fluorescence Cell Counter HL-60 50viability
        • HL-60 (50% viability)

          Experimental condition

          HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

        • HL-60 (high density)

          Experimental condition

          A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

        • Human peripheral blood

          Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.

        • Jurkat

          Experimental condition

          Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.

        • Mouse neuron

          Experimental condition

          Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.

        • LUNA-FL™ Dual Fluorescence Cell Counter Mouse-PBMC
        • Mouse PBMC

          Experimental condition

          Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.

        • LUNA-FL™ Dual Fluorescence Cell Counter Mouse-splenocyte
        • Mouse splenocyte

          Experimental condition

          Mouse splenocytes were collected directly from spleen tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.

        • LUNA-FL™ Dual Fluorescence Cell Counter Mouse-thymocyte
        • Mouse thymocyte

          Experimental condition

          Mouse thymocytes were collected directly from the thymus tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.

        • Saccharomyces Cerevisiae (50% viability)

          Experimental condition

          Yeast (Saccharomyces Cerevisiae) cells were cultured in the YPD medium supplemented with several antibiotics overnight. The yeast cells were artificially killed by heating and mixed with healthy cells (50:50). Mixed cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter.

        • LUNA-FL™ Dual Fluorescence Cell Counter Size-bead
        • Size bead

          Experimental condition

          A high density 3 micron-sized beads were counted with the LUNA-FL to verifiy the performance of the instrument. Beads were counted at the bright field cell counting mode of LUNA-FL. Orange circles represent the automatically-classified objects by LUNA-FL.

        • LUNA-FL™ Dual Fluorescence Cell Counter Yeasts in a messy culture
        • Yeasts in a messy culture

          Experimental condition

          The number and the viability of yeast cells were measured in a bottle of a Korean rice wine. The wine was diluted at 1:100 with PBS, and the cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter

      show more  +

      ORDERING INFORMATION

      Cat # Product Qty
      L20001 LUNA-FL™ Automated Fluorescence Cell Counter 1 unit
      L12005 PhotonSlide™, 50 Slides 1 box
      L12006 PhotonSlide™, 500 Slides 10 boxes
      L12007 PhotonSlide™, 1000 Slides 20 boxes
      L12001 LUNA™ Cell Counting Slides, 50 Slides 1 box
      L12002 LUNA™ Cell Counting Slides, 500 Slides 10 boxes
      L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 boxes
      L12011 LUNA™ Reusable Slide 1 unit
      L12012 LUNA™ Reusable Slides 2 units
      L12014 LUNA™ Reusable Slide Coverslips 10 units
      T13001 Trypan Blue Stain, 0.4% 2 X 1 mL
      L13002 Erythrosin B Stain 2 x 1 mL
      F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
      F23002 Acridine Orange Stain 2 x 0.5 mL
      F23003 Yeast Viability Kit 1
      - Propidium Iodide Stain for Yeast (1 x 0.5 mL)
      - Fluorescein Diacetate Stain (1 x 0.5 mL)
      - Cell Dilution Buffer (1 x 20 mL)
      - Fluorescence Signal Enhancer 1 (1 x 0.5 mL)
      1 kit
      F23212 Cell Dilution Buffer 5 x 20 mL
      F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
      B13101 LUNA™ Standard Beads 2 x 1 mL
      F24003 LUNA-FL™ IQ/OQ Protocol 1 copy
      P10001 LUNA™ Printer 1 unit
      P10002 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls