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Fluorescence Cell Counter
LUNA-FL™ Dual Fluorescence Cell Counter
Equipped with dual fluorescence and brightfield optics, the LUNA-FL™ is a powerhouse. The LUNA-FL™ can assess cell counts, viability, and GFP transfection efficiency without being limited by cell type or size.download software
WHAT THE LUNA-FL™ DOES BEST
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TWO COUNTERS IN ONE
The LUNA-FL™ can count in both brightfield and fluorescence mode, making
it our most versatile counter to date. -
COMPATIBLE WITH MOST CELLS
Advanced imaging software and GFP and RFP fluorescence optics sensitively
detect fluorescence stained cells. -
GFP TRANSFECTION ANALYSIS
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
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MAXIMUM 30 SECONDS
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
THE SOFTWARE THAT CHANGES EVERYTHING.

Cat # | Product | Qty | MSDS | Product Insert | ||||||
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B13101 | LUNA™ Standard Beads | 2 x 1 mL |
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T13001 | Trypan Blue Stain, 0.4% | 2 x 1 mL |
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L13002 | Erythrosin B Stain | 2 x 1 mL |
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F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
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F23002 | Acridine Orange Stain | 2 x 0.5 mL |
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WHAT DO OUR CUSTOMERS HAVE TO SAY?
Testimonials
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Berkan Savas
INSERM U955, IMRB Team Sahali
Saves great amount of times if you need to count your cells during the experiment.
Super easy to use, and comes with great explanatory leaflet for quick start. Very accurate results. -
Delphine Merle
CILOA
Reliable and user friendly.
Fast, reproducible and easy to use. Possibility to save protocols and export data. Helpfull to count GFP cells. -
Alessandro Devigili
University of Padova
Great results in short time given a proper starting set-up.
I used the LUNA system to estimate cell density and cell viability. In both cases, once the protocol is well defined and the experimental conditions extremely standardized, the system works very efficiently. It is less efficient in a non laboratory conditions. -
Sivaraman Natarajan
St jude childrens research hospital
I have been using this LUNA counter for the last 3 years, it's simple and easy.
The accuracy of cell count is very important for single-cell genomics experiments, in our hands the LUNA is very reliable and repeatable. -
Cécile Conrad
Institut Curie
Cell counter very convenient to use, accurate and fast.
It's very convenient to use, accurate and fast. The LUNA-FL can distinguish primary cells such as PBMCs, DCs from debris that's why we upgraded from the "classic LUNA" to the LUNA-FL. It's very helpful because we are not limited by cell type or size. In addition, the price is suitable so we're very pleased by that cell counter. -
Cécile Apolit
Abivax
An essential instrument for our studies.
We acquired the LUNA-FL in 2017 to replace an old cell counter and we are totally satisfied. We like: -Ease of use -The ability to specifically count PBMCs -The speed and availability of after-sales service -The price very correct. -
Corinne Rolland
IRSD
We are satisfied with the LUNA-FL.
The cell counter LUNA-FL is remarkably easy to use, fast and reliable; with a reactivity of the after-sales service. We are totally satisfied with the LUNA-FL. -
Clayton Wandishin
Vanderbilt University
Fast and accurate cell viability and counts.
The LUNA-FL's strength is in the viability accuracy, as well as being able to differentiate aggregated cells from single large cells due to the nature of the dual fluorescent dye. I use this machine constantly with cell culture and its speed, ease of use, cost, and small footprint were the major factors in purchasing this over another fluorescent counting option. -
Cem Sonmez
HistoGenetics
Peace of mind progress with cell counting.
We are using the LUNA-FL for counting mostly insect cells during the maintenance and expansion of the cell cultures. It`s operation is very straightforward once the settings are customized according to the cell type. Compared to bright light, fluorescence counting results in much more accurate (up to 10 million cells/ml) and reproducible results. With reusable slide, we don’t have to set focusing every time. It takes a minute or so to get the cell count and viability. Just in case, we always check the tagged cells under bright light and we don’t see any unlabeled or mislabeled cells, independent of cell size or cell clustering. -
Sarah Kristian
IU School of Medicine
Amazing customer service, love the instrument.
Our LUNA-FL Cell Counter was having some issues right out of the box. I communicated this to Logos and within the hour, I received a call to help troubleshoot. After attempting to troubleshoot and realizing the attempt was futile, Logos offered to send a replacement instrument within the week. I was extremely impressed by the customer service and I would absolutely buy from Logos again. The company takes pride in their instruments and was professional, courteous and fast to help! -
Tadeusz Karcz
NIEHS
Great for primary cells.
We use the LUNA-FL cell counter for analysis of mouse tissue preparations and primary cell cultures. Our samples include: lung homogenates, bronchoalveolar lavage fluid, T cells culture, etc. Acridine orange/propidium iodide dual staining is particularly beneficial for evaluation of samples contaminated with red blood cells. This approach is fast (analysis conducted immediately after addition of dyes) and more precise than methods utilizing trypan blue staining. Wide detection range and availability of re-usable slides are another advantages of the LUNA-FL. Major disadvantages include: no internal memory, only one USB port - precluding simultaneous use of pendrive (for saving results) and printer, long-lasting saving of results, lack of automatic focusing. -
Laura Fin
MRC-PPU, University of Dundee
Great cell counter, robust re-usable slides, incredible customer support!
We purchased this cell counter for our busy tissue culture facility. We needed a machine that is easy to use, fast, provides good reproducible results and offers the possibility to save funds on consumables by using re-usable counting slides instead of disposable ones. The LUNA-FL has all these features. The interface is user-friendly, the counter is easy to use with minimum training required and the results are almost immediate, clear and very reproducible. Moreover, users have appreciated the fluorescence function too. It gives a very quick and clear idea of the efficiency of transfection experiments. The possibility to use a re-usable slide with this counter has reduced our expenses towards consumables by an incredible amount. In general, we are very happy with our purchase. A final note has to be made for the incredible support of the customer service: we had a very useful demonstration of the machine, which was left at our facility for a month for trial, without any obligation of purchase. We had an initial big discount for the purchase of two machines, which included two re-usable slides free of charge. When our re-usable slides broke, Logos was upgrading the design and in the meantime we were offered disposable slides free of charge. We have been using the upgraded design for months now, without any problems. -
Larriana.S Saskironi
Ohio
Great but minor improvement needed.
This machine is one of the best .Easy to use and in most cases accurate. However, the application needs minor improvements regarding view on the monitor and speed of counting but overall a great device. -
Adeyemi Olanrewaju
George mason University
Great product with great results.
The product rarely get faulty as all the components are adequately packaged and gives results within few seconds, easy to use as well. -
Ritika Kukreja
Berkeley Lights Inc.
Thanks for the functional product!
This product is great - does what it says it will. Occasionally freezes, but since it works so well I don't care. -
Marishita Idris
Monash University Malaysia
User friendly & fast.
It is user friendly, fast & efficient. It only takes a few seconds to do cell counting and analysis with all info at the main display panel. I like the feature where I can zoom in up to 4x using this equipment. -
Vikram Rathore
Amity University
Highly useful.
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. -
Kun Liu
Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences
Great result & easy to use.
This machine is one of the best .Easy to use and in most cases accurate, rarely get faulty as all the components are adequately packaged and gives results within few seconds. -
Reza B
Kyoto University
Try it and determine if it will works fine for you application. Helpful and handy!
It's a compact device useful for cell counting but the camera and software can be much far better in this area of time as we have better resolution camera that can be fancy and much useful with cheaper price. In comparison to other cell counting device, it is comparable. There is a negative point of the design of the slide for cell counting about the depth that can focus better on nuclei counting. It can be faster and much reliable on the stability of the camera focus point for the next usage with the same cell line. -
Satomi Iwahashi
Mitsubishi Corporation Life Sciences
It makes culturing cells easier and faster.
It's very easy to use. If the cell counter had a Japanese version too, it will be better. -
F Abali
University of twente
An excellent and reliable piece of technology!
The cell counter is very user friendly, and provides reliable/fast cell count and cell viability. The cell counter is useful when you need to count a large number of samples in a short period of time. -
Haili Lang
Children's National Medical Center
Easy to use, great counting results.
I use it daily for cell counting. It is easy to use and fast to get accurate/reliable results. -
Fiona Leblay
Mivegec
Great purchase!
Very practical to use and very precise! In transfection procedures it's very helpful and speeds up our experiments a lot. I'm loving it! -
Soraya GROC
MIVEGEC, IRD
Good product, quick and efficient!
It is very useful to count quickly, in comparison with the cell of Malassez. It also allows you to have the number of dead cells and a living / dead cell ration. I highly recommend this product! -
Jet Spalink
Saxion hogescholen
Super, I use it every day to count my cells.
It is a really nice instrument to measure the amount of cells. It gives really quickly results. -
Magdalena Rausch
University of Geneva
Easy to use to obtain robust and reproducible results.
The LUNA-FL Dual Flourescence Cell Counter is easy to adjust and to use to obtain reproducible results. The high-resolution quality of the bright field and fluorescent images allows efficient and quick analysis of the cell count and cell viability. Once the parameters are set it is possible to obtain a robust read-out independent of the cell morphology. We use the LUNA-FL Dual Fluorescence Cell counter every day and it facilitates fast and user-friendly application. It is possible to save the bright field and fluorescent images on a portable drive for further quantification. -
Christian Breukers
University of Twente
A reliable and nice cell counting tool.
We have been use the LUNA-FL Cell Counter already for years and its ease of use and reliability make us happy users. -
Lluis Lopez-Barcons
University of Michigan
Easy to use, uses small space, reliable, no warm-up waiting.
Easy to use. Once you turn it on, it is ready to use, so no warm-up waiting. It counts cells pretty fast, in less than a minute. One problem is that for cells that strongly aggregate or are clumpy, readings are not accurate. -
Fanni Borvető
IRD
Fast and accurate, the product to have!
The LUNA-FL is easy to use and it gives reproducible results between cell counts. Moreover, it saves me a lot of time as each count is very fast and accurate! It is a good product to have in a cell lab. -
Arren Pineda
Parexel International
Great software interface and easy to use!
Setup and software interface is easy to use. Very sturdy product. -
Xicheng Ding
TAMU
Handle samples quickly.
Works easily. We like this labelling and try to do work with 3D culture. -
Zachary Niziolek
Flow Core
Excellent results, a key player in our cell counting pipeline.
Extremely reliable and useful. The ability to look at live/dead using the AO/PI stain gives the opportunity to ensure the correct cell concentration is being measured. -
Abhi Gadi
NYU school of medicine
Great value for money.
For our experiments, we use a lot of fluorescence cell counting. This instrument definitely helped us reduce the time we spend in achieving the results. -
Rana Al-Baghdadi
University of Al-Qadisiyah
It made it easy to to be in the lab.
It made it easy to be in the lab with good experience and high quality, reproducible results. -
Karan Singh
NYU School of medicine
it is a good instrument which will be very helpful for cell proliferation assays.
I am satisfied with this equipment. it is very easy to use. -
Kim Dong Ho
Genome&Company
Easy, fast and accurate machine!
Until this machine was used, the cells were directly counted and observed under a microscope. However, after using the LUNA-FL Dual Fluorescence Cell Counter, we were able to save about half the time and get accurate results. Very easy to use which is also an advantage of this machine. I recommend to everyone! -
Hugh Kromer
Cellarity, Inc.
Great cell counting machine.
The LUNA-FL works very well for counting cells using trypan blue. It is able to recognize live and dead cells in many different types of cells. -
Danny KItsberg
Hebrew University of Jerusalem
A very versatile, easy to use cell counter with many useful functions.
It is a very nifty fluorescent cell counter. Very easy to use and with high reproducibility. Many useful functions for gating, cell size measurement and for counting yeast. I have used other counters but this one surpasses them all. We are very happy that we introduced it into our lab. Highly recommended! -
Gabrielle Cannon
UNC
Ensures scRNA-Seq Success.
This instrument has been indispensable for our single cell RNA-Seq sample prep process. Most of our samples are primary cells that have a high potential for debris and cell aggregates, which make traditional hemocytometer/trypan counting difficult. Dual fluorescence counting has greatly improved our accuracy and reliability. We also love being able to digitally archive the images/count reports for later reference. The instrument controls are intuitive and allow for manual focusing of the cell images. Our service rep has been very quick to address our after-sales needs. The LUNA-FL is the most affordable instrument that meets our needs (and often exceeds them!). Would recommend. -
Kathy Evans
DUKE INTERNAL MEDICINE ONCOLOGY
Accurate and easy to use.
We are a research lab that does in vitro and in vivo immunological studies. Viability and accurate cell counts are extremely important to our studies. The LUNA-FL is extremely user friendly and easy to switch from brightfield to fluorescence to GFP. The option to use a resusable slide also makes this instrument cost effective when only counting one sample. It takes up very little bench space and fits in our small tissue culture room where space is a premium. The ability to demo the LUNA instruments side by side for as long as we needed was a must for us and Logos allowed us the time and support to test all counting methods completely. -
Kevin De Muynck
Ghent University
Fast, rapid, reliable counting with AO/PI.
From 10 ml whole blood it is estimated to obtain roughly 10 million PBMCs. When counting with the LUNA-FL with the AO/PI stain, we indeed see total cell counts approximating what we expected from what is known. This system in combo with this dye mix offers a fast, reliable solution to count PBMCs. -
Samson Omole
GMU
Easy to use, saves time and is very efficient.
The equipment is very reliable with great accuracy and precision. It is user friendly and it is easy to use. In my former lab, we counted cells via microscope using the counting chamber with which it was very difficult to identify dead cells. With this equipment my job has been made easy. -
Jaeda Roberts
Pritzker School for Molecular Engineering
Easy to use!
The cell counter is easy to use, great for teaching, and very accurate most of the time. Use the bright field setting and the fluorescent setting most frequently and am satisfied with both. -
Patrick Mccarthy
Covaris
Very accurate and easy to use.
The LUNA-FL Dual Fluorescence Cell Counter is very fast and easy to use. Our lab utilizes this instrument to count multiple cell types of different sizes; I much prefer it to a hemocytometer. The LUNA-FL is small and easily fits on our bench top. The counting and viability data is accurate and reproducible. -
Colleen Olkowski
NIH
No need to look for any other counter.
No need to look anywhere else for a cell counter. This is used by every group. It's very easy to use, and the ability to save count reports on different drives makes life so much easier than hand-recording everything. -
Madeline Terpilowski
Usc
Great results, use it daily.
This product is great I use it multiple times a day and it’s always consistent and useful. It’s my favorite cell counter in the lab! Highly recommend! -
Niko Kangasniemi
UH
Easy to use product.
Used to count living/dead cells after tissue digestion. Great service and easy to use product! -
Eric M
NIH
Reliable, easy-to-use, compact and accurate - highly recommend!
Our lab has been using the LUNA-FL Dual Fluorescence Cell Counter for almost a year now and we have nothing but great things to say about it. Operating it is extremely straightforward with its simple and user-friendly interface. In addition to providing quick and accurate results, it includes many customizable features for all of your cell counting needs. -
Rebecca L
UNC
Reliable and easy to use.
This cell counter is extremely easy to use and has provided quick and accurate results on a consistent basis. It saves a lot of time and doesn't take up much lab space. I would highly recommend this product to anyone looking for an accurate cell counter. -
Aizhan Tastanova
University Hospital Zurich
Simplifies the procedure of preparing single-cell suspension.
Easy-to-use and user-friendly interface. A must have tool for labs that have a lot of cell culture work and do a lot of viability assays. The customer service was amazing - gave us two demo tests. -
Ondřej Vymazal
FNUSA
Thank you for this product, it is really helping me.
It is not easy to count neutrophils, and it takes so long time do it manually. When I am using the LUNA-FL with PhotonSlide, it is a very easy, fast and precise count. -
Nicholas Wameling
Antibody Solutions
Best cell counting instrument on the market!
We use our LUNA-FL Dual Fluorescence Cell Counter every day for our flow assays and other duties. The first thing I noticed about the LUNA-FL is that it is very easy to use. Sometimes we are counting multiple cell types and the LUNA-FL is quick and very accurate. The LUNA cell counting slides we use are double sided, which helps to reduce waste, and they are of high quality. Overall this product has no flaws in my opinion. -
Nicholas LeFlohic
ImmunoActiva, Inc.
Great results and takes no time at all to use!
The Cell Counter is effective for allowing us to make better use of our time instead of using manual counting methods like the hemacytometer. There is some variability to the results, however they are for the most part consistent. The Cell Counter is intuitive in its interface and easy to operate with minimal training. One should be mindful of dilutions. So far we've had no problem (sourced to the equipment) and have had many instances of its reliable use. -
Christoph Hapke
Charité Comprehensive Cancer Center
Good results, easy to use.
The LUNA-FL is a very compact, lightweight device with an intuitive user interface that only requires minimal training. We use it every day for live/dead cell counting and are satisfied with the results. Logos offers disposable slides for high-throughput but also reusable ones for smaller labs (with a little effort you can also reuse the disposable ones but you didn't hear that from me). Preparation of slides with AO/PI takes about 1 minute. -
Debjani Saha
Takeda ireland Limited
Great results, saves time!
The cell counter is very effective and saves time instead of using a hemocytometer. -
Cristina Brischetto
Max Delbruck Center
Fast and easy to use!
Fast and useful cell counter! LUNA-FL is an optimal automated cell counter, easy to use and reliable. I am really happy with this product and I would absolutely recommend it. Save your time and make differences to your experiments. -
Ilaria Malpighi
Ospedale San Raffaele
Precise, compact, intuitive!
We use LUNA-FL Cell Counter to count different cell lines, allowing us to save valuable time, and to have precise and reproducible results. Easy to use, with minimal training. Compact size, in fact it can be put in a drawer. Fast reading, with a detailed outcomes. -
Alba Medina
Hospital S. Pau
Very fast and complete solution.
We use it as a cell counter. It has many functions, it's very fast and takes up very little space. It uses very little sample and gives a lot of information. -
Ahmet Bugra Tufan
Max Delbruck Center for Molecular Medicine
Great results.
It was an amazing cell counter. It helped us a lot and saved a lot of time. -
Rahul Biradar
Organization: Turku Bioscience
Enjoy the joy of counting cellsEnjoy the joy of counting cells.
Among the best cell counters I have used so far! Easy and quick to use. One can export the high-quality images of cell counting field for references. The cell counting is quite precise. -
Chiang Yao-an
Organization: Medigen Vaccine Biologics Corp
Easy to use and good results.
It's very fast and takes up very little space. I'm really happy with this product and I would absolutely recommend it. It is easy to count PBMC. -
Xiao Ting Qiu
High-end vaccine biologics
A simple and time-saving cell counter.
It is very convenient for counting cells and save time. You can see the cells on the screen and recalculate the cells with the "tag" key. The results can also be output as a PDF file for records and reports. -
Yogesh Taparia
Ben-Gurion University of The Negev
Excellent device for quick and accurate cell-counts.
We use LUNA-FL for counting diatom cells routinely in our lab. Its extremely convenient and fast for taking cell counts which are reliable and robust. We have been using the LUNA-FL for about 3 years and have had no problems with this device. Highly recommend it for routine labwork. -
Melita Videja
Latvian Institute of Organic synthesis
From very simple use to rapid analysis.
LUNA-FL has helped us in day-to-day work and provides even more opportunities than hoped. Dual fluorescence optics help us obtain more accurate and reliable results, representative images, and high quality on-board analysis. Histogram data with cell size distribution and cell-cluster graphs are very important because we are using various cell lines and primary cells as well. Great value for money! -
Kārlis Vilks
Latvian Institute of Organic Synthesis
Nice device for saving time and sanity.
Easy to use and very useful for determination of transfection efficiency using GFP transfection assays. -
Olajumoke Babatunde
Western Illinois University
Great instrument.
The instrument is reliable and does counting perfectly. -
Matthew Crawford
University of Virginia
Accurate results, easy to use, great sales and follow up support.
We purchased the Logos Biosystems LUNA-FL cell counter to quantitate human T lymphocytes and other cell types. This was early 2020, so most everything was shut down due to the pandemic, but we needed a good cell counter for SARS-CoV-2 research. To test if the instrument would meet our needs, Logos shipped us a demo to try out. The machine is very easy to use, accurate, and provides lots of supplemental data beyond just cell number. The LUNA-FL cost a bit more than some comparable counters, but less than others. The sales support and flexibility was what really stood out and sold our lab. The disposable slides can get expensive when performing many counts, but that's a common refrain for most counters. To save on the costs of consumables, I recommend buying straight from Logos; reusable slides also exist. All-in-all, this instrument, and the support behind it, have met our lab's needs well. We're happy and without complaints. -
Claire Morton
RxCelerate
Great bit of kit.
The LUNA-FL cell counter is a great bit of kit, it has really saved us a lot of time in the lab. It's compact, space saving and intuitive. It's great having the flexibility of having both brightfield and fluorescence cell counting in one machine. -
Kevin Lee
University of California Irvine
Great. Easy to use.
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Mike Licudi
Imperial College London
I love the LUNA-FL counter. It's so easy to use and it's fast - which is important for my applications when I can't have cells on ice for too long. I love the fact that there are multiple counting modes (dual fluorescence or brightfield) and you can set it up to use your custom stains. Getting practically instant cell counts with viability and dilution calculations done automatically is excellent. The other huge advantage over other automated counting systems is that you can visualize your cells on the screen to ensure they're healthy and spot any anomalies, which you can't do with cuvette based counters for example. It's also great that it stores all the data and can export it to USB, including cell images too. Very easy to calibrate, also.
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Hugo Sugier
Aenitis technologies
Great device, user-friendly and time saving.
We use this cell counter to count THP1 cell lines, cultured mesenchymal stromal cells and, thanks to its fluorescence, stromal cells from stromal vascular fractions. The results are more consistent than with a classic hemocytometer. The device is really easy to use and displays the picture of the counting, allowing you to check what counted as a cell or not. You even have a graph with average sizes of the cells. Logos is a company close to their clients and they are very helpful. To conclude: No regret, and time saved! -
Huang Kirk
Taiwan Color Optics
Great product specialists in Taiwan.
Thanks for good product specialists in Taiwan. They helped solve our counting problems. -
Mengxian Zhang
Tsinghua University
Great! Useful instrument to count cells and test viability.
Easy to use. A little bit expensive. -
Soe Min
Memorial Sloan Kettering Cancer Center
100% recommended!
This is my very first time using it for cell counting. It is very easy to use, effective, time-saving and reliable. -
Elke Reinhardt
Sanofi
Fast and sparing with my cells.
Very convenient cell counter. Does not need much space on the bench and results are reliable (checked in comparison to FACS analysis. It is great that only a small amount of cell suspension is used to get quick results! -
Yengjey Yang
Medigen
Worth buying!
LUNA-FL Dual Flurescenece cell counter elevate the efficiency of counting cell and increase our trust to machine counting system. -
Koen De Wispelaere
VIB-KUL
Really handy, using it on a daily basis.
Good stuff, easy to use and compact. -
Brandie Fullmer
LBRI
Easiest cell counter to use on the market.
By far the easiest cell counter to use on the market. It clearly tags live and dead cells so the user can check the accuracy of the count. The user does not have to make a ton of adjustments for accurate counting like other instruments. -
Gelina Sani
Children's National Medical Center
The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.
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Mikayla Lopes
Roger Williams Medical Center
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!
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Regina Wulff, MS
Weill Cornell Medicine / New York-Presbyterian Hospital
The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
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Danh Tran
Medical University of South Carolina
Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.
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Woong Sun, PhD
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
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May Malicdan, MD PhD
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.
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Joanna Ireland, PhD
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
WHERE THE LUNA-FL™ HAS BEEN CITED
Citations
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Inhibiting calpain 1 and 2 in cyclin G associated kinase–knockout mice mitigates podocyte injury.
2020. Tian X, Inoue K, Zhang Y, Wang Y, Sperati CJ, Pedigo CE, Zhao T, Yan M, Groener M, Moledina DG, Ebenezer K, Li W, Zhang Z, Liebermann DA, Greene L, Greer P, Parikh CR, Ishibe S. JCI Insight 5(22):e142740. -
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19.
2020. Su Y, Chen D, Yuan D, Lausted C, Choi J, Dai CL, Voillet V, Duvvuri VR, Scherler K, Troisch P, Baloni P, Qin G, Smith B, Kornilov SA, Rostomily C, Xu A, Li J, Dong S, Rothchild A, Zhou J, Murray K, Edmark R, Hong S, Heath JE, Earls J, Zhang R, Xie J, Li S, Roper R, Jones L, Zhou Y, Rowen L, Liu R, Mackay S, O'Mahony DS, Dale CR, Wallick JA, Algren HA, Zager MA; ISB-Swedish COVID19 Biobanking Unit, Wei W, Price ND, Huang S, Subramanian N, Wang K, Magis AT, Hadlock JJ, Hood L, Aderem A, Bluestone JA, Lanier LL, Greenberg PD, Gottardo R, Davis MM, Goldman JD, Heath JR. Cell 183(6):1479-1495, e20. -
Baculovirus-vectored precision delivery of large DNA cargoes in human genomes.
2020. Aulicino F, Pelosse M, Toelzer C, Capin J, Meysami P, Dillingham MS, Schaffitzel C, Berger I. Biorxiv 2020.08.17.253898. -
Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without.
2020. Theuerkauf SA, Michels A, Riechert V, Maier TJ, Flory E, Cichutek K, Buchholz CJ. Biorxiv 2020.10.15.340604. -
Identification of genomic enhancers through spatial integration of single‐cell transcriptomics and epigenomics.
2020. Bravo González-Blas C, Quan XJ, Duran-Romaña R, Taskiran II, Koldere D, Davie K, Christiaens V, Makhzami S, Hulselmans G, de Waegeneer M, Mauduit D, Poovathingal S, Aibar S, Aerts S. Molecular Systems Biology 16(5):e9438. -
Safety and Tolerability of Stromal Vascular Fraction Combined with β-Tricalcium Phosphate in Posterior Lumbar Interbody Fusion: Phase I Clinical Trial.
2020. Choi UY, Kim KT, Kim KG, Lim SH, Kim YJ, Sohn S, Sheen SH, Heo CY, Han I. Cells 9(10):2250. -
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Human Mesenchymal Stem Cell Hydrogen Sulfide Production Critically Impacts the Release of Other Paracrine Mediators After Injury.
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Single-cell transcriptome analysis of the Akimba mouse retina reveals cell-type-specific insights into the pathobiology of diabetic retinopathy.
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Uptake and excretion dynamics of gold nanoparticles in cancer cells and fibroblasts.
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2020. Duroy PO, Bosshard S, Schmid-Siegert E, Neuenschwander S, Arib G, Lemercier P, Masternak J, Roesch L, Buron F, Girod PA, Xenarios I, Mermod N. Biotechnology and Bioengineering 117(2):466‐485. -
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Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy.
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Furin-mediated intracellular self-assembly of olsalazine nanoparticles for enhanced magnetic resonance imaging and tumour therapy.
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2019. Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, Artavanis-Tsakonas S, Youle RJ, Pickrell AM. Cell Reports 29(1): 225-235. -
Engineered fibrillar fibronectin networks as three‐dimensional tissue scaffolds.
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2019. Jankowski W, McGill J, Lagassé HAD, Surov S, Bembridge G, Bunce C, Cloake E, Fogg MH, Jankowska KI, Khan A, Marcotrigiano J, Ovanesov MV, Sauna ZE. Blood Advances 3(17): 2668-2678. -
A comparison of ovine facial and limb muscle as a primary cell source for engineered skeletal muscle.
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2019. Traber KE, Dimbo EL, Symer EM, Korkmaz FT, Jones MR, Mizgerd JP, Quinton LJ. PLoS One 14(8): e0221029. -
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Thrombin preconditioning of extracellular vesicles derived from mesenchymal stem cells accelerates cutaneous wound healing by boosting their biogenesis and enriching cargo content.
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Homotypic and heterotypic trans-assembly of human Rab-family small GTPases in reconstituted membrane tethering.
2019. Segawa K, Tamura N, Mima J. Journal of Biological Chemistry 294: 7722-7739. -
Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells.
2019. Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Mizuguchi H, Sato Y. Scientific Reports 9(1): 3630. -
Chimeric antigen receptor costimulation domains modulate human regulatory T cell function.
2019. Boroughs AC, Larson RC, Choi BD, Bouffard AA, Riley LS, Schiferle E, Kulkarni AS, Cetrulo CL, Ting D, Blazar BR, Demehri S, Maus MV. JCI Insight 4(8): e126194. -
Exploiting evolutionary herding to control drug resistance in cancer.
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2018. Filonov D, Tice R, Luo R, Grotegut C, Van Kanegan MJ, Ludlow JW, Il'yasova D, Kinev A. Frontiers in Public Health 6: 369. -
Predicted glycosyltransferases promote development and prevent spurious cell clumping in the choanoflagellate S. rosetta.
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Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy.
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CD19-directed CAR T-cell (CTL019) Product Viability and Clinical Outcomes in Non-Hodgkin Lymphomas and B-cell acute lymphoblastic leukemia.
2018. Chong EA, Levine BL, Grupp SA, Davis M, Siegel DL, Maude SL, Gladney WL, Frey NV, Porter DL, June CH, Schuster SJ. Blood 132 (Suppl 1): 197. -
Zooming in on cryopreservation of hiPSCs and neural derivatives: A dual-center study using adherent vitrification.
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Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival.
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Development of new 3D human ex vivo models to study sebaceous gland lipid metabolism and modulations.
2018. de Bengy AF, Forraz N, Danoux L, Berthelemy N, Cadau S, Degoul O, Andre V, Pain S, McGuckin C. Cell Proliferation 52(1): e12524. -
Surface modification of macrophages with nucleic acid aptamers for enhancing the immune response against tumor cells.
2018. Sugimoto S, Iwasaki Y. Bioconjugate Chemistry 29(12): 4160-4167. -
Association of the human bocavirus with tonsil squamous cell carcinomas.
2018. Höpken M, Förster I, Maune S, Brockmann M, Schildgen O, Schildgen V. Frontiers in Microbiology 9: 2450. -
Pregn-5-en-3β-ol and androst-5-en-3β-ol dicarboxylic acid esters as potential therapeutics for NMDA hypofunction: In vitro safety assessment and plasma stability.
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Choanoflagellate transfection illuminates their cell biology and the ancestry of animal septins.
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2018. De Schepper S, Verheijden S, Aguilera-Lizarraga J, Viola MF, Boesmans W, Stakenborg N, Voytyuk I, Schmidt I, Boeckx B, Dierckx de Casterlé I, Baekelandt V, Gonzalez Dominguez E, Mack M, Depoortere I, De Strooper B, Sprangers B, Himmelreich U, Soenen S, Guilliams M, Vanden Berghe P, Jones E, Lambrechts D, Boeckxstaens G. Cell 175(2): 400-415. -
Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow.
2018. Sasaki A, Mizuno M, Ozeki N, Katano H, Otabe K, Tsuji K, Koga H, Mochizuki M, Sekiya I. PLoS One 13(8): e0202922. -
Effect of the bowl structure in an automated cell-isolation device on stromal vascular fraction's isolation yield.
2018. Hahn HM, Jeong KS, Yoo BY, Park JH, Jung HJ, Lee IJ. Journal of Medical Devices 12(4): 044501. -
A single-cell transcriptome atlas of the aging Drosophila brain.
2018. Davie K, Janssens J, Koldere D, De Waegeneer M, Pech U, Kreft Ł, Aibar S, Makhzami S, Christiaens V, Bravo González-Blas C, Poovathingal S, Hulselmans G, Spanier KI, Moerman T, Vanspauwen B, Geurs S, Voet T, Lammertyn J, Thienpont B, Liu S, Konstantinides N, Fiers M, Verstreken P, Aerts S. Cell 174(4): 982-998. -
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
2018. Reily C, Rizk DV, Julian BA, Novak J. Biotechniques 65(2): 71-77. -
Hair cell mechanotransduction regulates spontaneous activity and spiral ganglion subtype specification in the auditory system.
2018. Sun S, Babola T, Pregernig G, So KS, Nguyen M, Su SM, Palermo AT, Bergles DE, Burns JC, Müller U. Cell 174(5): 1247-1263. -
HIV-1 targets L-selectin for adhesion and induces its shedding for viral release.
2018. Kononchik J, Ireland J, Zou Z, Segura J, Holzapfel G, Chastain A, Wang R, Spencer M, He B, Stutzman N, Kano D, Arthos J, Fischer E, Chun TW, Moir S, Sun P. Nature Communications 9(1): 2825. -
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2018. Sammaibashi S, Yamayoshi S, Kawaoka Y. Frontiers in Microbiology 9: 1446. -
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2018. Nair N, Gomes-Solecki M. bioRxiv 334219. -
FGFR signaling regulates resistance of head and neck cancer stem cells to cisplatin.
2018. McDermott SC, Rodriguez-Ramirez C, McDermott SP, Wicha MS, Nör JE. Oncotarget 9(38): 25148-25165. -
Canine mesenchymal stem cells treated with TNF-α and IFN-γ enhance anti-inflammatory effects through the COX-2/PGE2 pathway.
2018. Yang HM, Song WJ, Li Q, Kim SY, Kim HJ, Ryu MO, Ahn JO, Youn HY. Research in Veterinary Science 119: 19-26. -
Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration.
2018. Nguyen JT, Ray C, Fox AL, Mendonça DB, Kim JK, Krebsbach PH. Science Advances 4(5): eaao5838. -
S1P₄ regulates passive systemic anaphylaxis in mice but is dispensable for canonical IgE-mediated responses in mast cells.
2018. Kulinski JM, Proia RL, Larson EM, Metcalfe DD, Olivera A. International Journal of Molecular Sciences 19(5): 1279. -
Mechanical tension drives elongational growth of the embryonic gut.
2018. Chevalier NR, de Witte TM, Cornelissen AJM, Dufour S, Proux-Gillardeaux V, Asnacios A. Scientific Reports 8: 5995. -
Dual mechanism of type VII collagen transfer by bone marrow mesenchymal stem cell extracellular vesicles to recessive dystrophic epidermolysis bullosa fibroblasts.
2018. McBride JD, Rodriguez-Menocal L, Candanedo A, Guzman W, Garcia-Contreras M, Badiavas EV. Biochimie 155: 50-58. -
Pan-cancer genome and transcriptome analyses of 1,699 paediatric leukaemias and solid tumours.
2018. Ma X, Liu Y, Liu Y, Alexandrov LB, Edmonson MN, Gawad C, Zhou X, Li Y, Rusch MC, Easton J, Huether R, Gonzalez-Pena V, Wilkinson MR, Hermida LC, Davis S, Sioson E, Pounds S, Cao X, Ries RE, Wang Z, Chen X, Dong L, Diskin SJ, Smith MA, Guidry Auvil JM, Meltzer PS, Lau CC,, Perlman EJ, Maris JM, Meshinchi S, Hunger SP, Gerhard DS, Zhang J. Nature 555(7696): 371-376. -
B cell receptor and CD40 signaling are rewired for synergistic induction of the c-Myc transcription factor in germinal center B cells.
2018. Luo W, Weisel F, Shlomchik MJ. Immunity 48(2): 313-326. -
Improving the quality of a recombinant rabbit monoclonal antibody against PLXDC2 by optimizing transient expression conditions and purification method.
2018. Shimizu H, Nakagawa M, Todaka N, Imaizumi K, Kurosawa Y, Maruyama T, Okumura CJ, Shibata T, Tanaka Y, Sato Y, Ono Y, Akuta T. Protein Expression and Purification 146: 27-33. Oncotarget 8(65): 109358-109369. -
Venous endothelium reactivity to Angiotensin II: A study in primary endothelial cultures of rat vena cava and portal vein.
2018. Trindade MR, Assunção HCR, Torres TC, Bertolino JS, Fernandes L. Experimental Cell Research 362(1): 188-194. -
Enhancement of recombinant antibody expression level by growth controlled medium.
2018. Phuc LTM, Sasaki T, Shimizu H, Huyen NTM, Thuy NTT, Huan LQ, Taniguchi A. Open Biotechnology Journal 12: 78-85. -
Dysregulated connexin 43 in HER2-positive drug resistant breast cancer cells enhances proliferation and migration.
2017. Yeh ES, Williams CJ, Williams CB, Bonilla IV, Klauber-DeMore N, Phillips SL. Oncotarget 8(65): 109358-109369. -
Lovastatin induced Kruppel like factor 2 (KLF2), Kruppel like factor 6 (KLF6) and Ras homolog family member B (RHOB) genes and preferentially led to viability reduction of Cisplatin-resistant cells.
2017. Koi C, Izumi H, Kurita T, Nguyen TT, Murakami M, Yoshiura Y2, Hachisuga T, Morimoto Y. Oncotarget 8(63): 106429-106442. -
Genome-wide segregation of single nucleotide and structural variants into single cancer cells.
2017. Easton J, Gonzalez-Pena V, Yergeau D, Ma X, Gawad C. BMC Genomics 18(1): 906. -
Molecular architecture underlying fluid absorption by the developing inner ear.
2017. Honda K, Kim SH, Kelly MC, Burns JC, Constance L, Li X, Zhou F, Hoa M, Kelley MW, Wangemann P, Morell RJ, Griffith AJ. eLife 6: e26851. -
Human induced pluripotent stem cell-derived cardiomyocyte encapsulating bioactive hydrogels improve rat heart function post myocardial infarction.
2017. Chow A, Stuckey DJ, Kidher E, Rocco M, Jabbour RJ, Mansfield CA, Darzi A, Harding SE, Stevens MM, Athanasiou T. Stem Cell Reports 9(5): 1415-1422. -
Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury.
2017. Lee SH, Kim Y, Rhew D, Kim A, Jo KR, Yoon Y, Choi KU, Jung T, Kim WH, Kweon OK. Journal of Veterinary Science 18(3): 377-386. -
Immunization with recombinant TcdB-encapsulated nanocomplex induces protection against Clostridium difficile challenge in a mouse model.
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Complete human serum maintains viability and chondrogenic potential of human synovial stem cells: suitable conditions for transplantation.
2017. Mizuno M, Katano H, Otabe K, Komori K, Kohno Y, Fujii S, Ozeki N, Horie M, Tsuji K, Koga H, Muneta T, Sekiya I. Stem Cell Research & Therapy 8: 144. -
Cell cycle synchronization and analysis of apoptosis‐related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna).
2017. Veraguas D, Gallegos PF, Castro FO, Rodriguez-Alvarez L. Reproduction in Domestic Animals 52(5): 881-889. -
Morusin induces apoptosis by regulating expression of Bax and Survivin in human breast cancer cells.
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MiR-34a-3p alters proliferation and apoptosis of meningioma cells in vitro and is directly targeting SMAD4, FRAT1 and BCL2.
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Self-organising aggregates of zebrafish retinal cells for investigating mechanisms of neural lamination.
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Tissue transglutaminase activates cancer-associated fibroblasts and contributes to gemcitabine resistance in pancreatic cancer.
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An enclosed rotating floating photobioreactor (RFP) powered by flowing water for mass cultivation of photosynthetic microalgae.
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Pleiotropic roles of the matricellular protein Sparc in tendon maturation and ageing.
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Premedication with clarithromycin is effective against secondary bacterial pneumonia during influenza virus infection in a pulmonary emphysema mouse model.
2016. Harada T, Ishimatsu Y, Hara A, Morita T, Nakashima S, Kakugawa T, Sakamoto N, Kosai K, Izumikawa K, Yanagihara K, Mukae H, Kohno S. The Journal of Pharmacology and Experimental Therapeutics 358(3): 457-63. -
Human adipose stromal cells increase survival and mesenteric perfusion following intestinal ischemia and reperfusion injury.
2016. Jensen AR, Doster DL, Hunsberger EB, Manning MM, Stokes SM, Barwinska D, March KL, Yoder MC, Markel TA. Shock 46(1): 75-82. -
In vitro endothelialization test of biomaterials using immortalized endothelial cells.
2016. Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S. PloS One 11: e0158289. -
High-throughput single-cell derived sphere formation for cancer stem-like cell identification and analysis.
2016. Chen YC, Ingram PN, Fouladdel S, McDermott S, Azizi E, Wicha MS, Yoon E. Scientific Reports 6: 27301. -
Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site.
2016. Mun JY, Shin KK, Kwon O, Lim YT, Oh DB. Biomaterials 101: 310-320. -
High antiviral effects of hibiscus tea extract on the H5 subtypes of low and highly pathogenic avian influenza viruses.
2016. Baatarsogt T, Bui VN, Trinh DQ, Yamaguchi E, Gronsang D, Thampsaisarn R, Ogawa H, Imai K. The Journal of Veterinary Medical Science 78(9): 1405-1411. -
Antidepressant imipramine diminishes stress-induced inflammation in the periphery and central nervous system and related anxiety- and depressive-like behaviors.
2016. Ramirez K, Sheridan JF. Brain, Behavior, and Immunity 57:293-303. -
Harvest tissue source does not alter the protective power of stromal cell therapy following intestinal ischemia and reperfusion injury.
2016. Jensen AR, Manning MM, Khaneki S, Drucker NA, Markel TA. The Journal of Surgical Research 204(2): 361-370. -
Human induced pluripotent stem cell-derived cardiomyocytes recapitulate the prediction of breast cancer patients to doxorubicin-induced cardiotoxicity.
2016. Burridge PW, Li YF, Matsa E, Wu H, Ong SG, Sharma A, Holmström A, Chang AC, Coronado MJ, Ebert AD, Knowles JW, Telli ML, Witteles RM, Blau HM, Berstein D, Altman RB, Wu J. Nature Medicine 22(5): 547-556. -
Tanshinone IIA induces TRAIL sensitization of human lung cancer cells through selective ER stress induction.
2016. Kim EO, Kang SE, Im CR, Lee JH, Ahn KS, Yang WM, Um JY, Lee SG, Yun M. International Journal of Oncology 48(5): 2205-12. -
Local biological effects of adipose stromal vascular fraction delivery systems after subcutaneous implantation in a murine model.
2016. Vigani B, Mastracci L, Grillo F, Perteghella S, Preda S, Crivelli B, Antonioli B, Galuzzi M, Tosca MC, Marazzi M, Torre ML, Chlapanidas T. Journal of Bioactive and Compatible Polymers 31(6): 600-612. -
Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform.
2016. Wagner T, Greschik H, Burgahn T, Schmidtkunz K, Schott AK, McMillan J, Baranauskiene L, Xiong Y, Fedorov O, Jin J, Oppermann U, Matulis D, Schüle R, Jung M. Nucleic Acids Research 44(9): e88. -
Broncholaveolar lavage to detect cytomegalovirus infection, latency, and reactivation in immune competent hosts.
2016. Mansfield S, Dwivedi V, Byrd S, Trgovcich J, Griessl M, Gutknecht M, Cook C. Journal of Medical Virology 88(8): 1408-1416. -
Morusin induces TRAIL Sensitization by regulating EGFR and DR5 in human glioblastoma cells.
2016. Park D, Ha IJ, Park SY, Choi M, Lim SL, Kim SH, Lee JH, Ahn KS, Yun M, Lee SG. Journal of Natural Products 79(2): 317-323. -
The relationship between antral follicle count in a bovine ovary and developmental competence of in vitro-grown oocytes derived from early antral follicles.
2016. Nagai K, Yanagawa Y, Katagiri S, Nagano M. Biomedical Research 37(1): 63-71. -
Promotion of cortical neurogenesis from the neural stem cells in the adult mouse subcallosal zone.
2016. Kim JY, Choi K, Shaker MR, Lee JH, Lee B, Lee E, Park JY, Lim MS, Park CH, Shin KS, Kim H, Geum D, Sun W. Stem Cells 34(4): 888-901. -
Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles.
2016. Yang Y, Kanno C, Huang W, Kang SS, Yanagawa Y, Nagano M. Reproductive Biology and Endocrinology 14:3. -
Generation of atmospheric-pressure dry- and mist-plasma jets and their effects on HeLa cells.
2016. Sonoda T, Umeda K, Wang D, Namihira T, Akiyama H. Plasma Medicine 5(2–4): 271–281. -
Application of a non-hazardous vital dye for cell counting with automated cell counters.
2016. Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical Biochemistry 492: 8-12. -
Mouse model for sublethal Leptospira interrogans infection.
2015. Richer L, Potula HH, Melo R, Vieira A, Gomes-Solecki M. Infection and Immunity 83(12): 4693-700. -
GABAergic modulation with classical benzodiazepines prevent stress-induced neuro-immune dysregulation and behavioral alterations.
2016. Ramirez K, Niraula A, Sheridan JF. Brain, Behavior, and Immunity 51:154-68. -
Human mesenchymal stromal cells decrease mortality following intestinal ischemia and reperfusion injury.
2015. Markel TA, Crafts TD, Jensen AR, Husberger EB, Yoder MC. The Journal of Surgical Research. 199(1): 56-66. -
Differential regulation of gene expression of alveolar epithelial cell markers in human lung adenocarcinoma-derived A549 clones.
2015. Kondo H, Miyoshi K, Sakiyama S, Tangoku A, Noma T. Stem Cells International. 2015: 165867. -
Expression analyses revealed thymic stromal co-transporter/Slc46A2 is in stem cell populations and is a putative tumor suppressor.
2015. Kim KY, Lee G, Yoon M, Cho EH, Park CS, Kim MG. Molecules and Cells 38(6): 548-61. -
Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6.
2015. Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I. International Journal of Oncology. 47(1): 122-32. -
β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells.
2015. Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I. Experimental & Molecular Medicine. 47: e143. -
Imipramine attenuates neuroinflammatory signaling and reverses stress-induced social avoidance.
2015. Ramirez KG, Shea DT, McKim DB, Reader BF, Sheridan JF. Brain, Behavior, and Immunity 46: 212-20. -
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images.
2013. Hong D, Lee G, Jung NC, Jeon M. Biological Procedures Online 15(1): 13.
Resources
Downloads
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Logos Biosystems Products
Brochure
2020-04-26 | 2.14 MB -
LUNA-FL™
Brochure
2018-04-18 | 2.1 MB -
LUNA-FL™: GFP Transfection Assay
Quick Start Guide
2018-04-16 | 110 KB -
LUNA-FL™
Quick Start Guide
2018-04-16 | 284 KB -
Fast automated yeast cell counting algorithm using bright-field and fluorescence microscopic images
Product literature
2018-04-16 | 1.7 MB -
GFP expression analysis using the LUNA-FL™
Product literature
2018-04-16 | 403 MB -
Fast cell counting and viability measurement of yeast cells with the LUNA-FL™
Product literature
2018-04-16 | 862 KB -
Automated fluorescence cell counting
Product literature
2018-04-16 | 476 KB -
Application of a non-hazardous vital dye for cell counting with automated cell counters
Product literature
2018-04-13 | 906 KB -
Using the LUNA Reusable Slide for accurate cell counting with automated cell counters
Product literature
2018-04-13 | 665.84 KB
Videos
Cell DB
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HL-60 (50% viability)
Experimental condition
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
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HL-60 (high density)
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
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Human peripheral blood
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
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Jurkat
Experimental condition
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
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Mouse neuron
Experimental condition
Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
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Mouse PBMC
Experimental condition
Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
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Mouse splenocyte
Experimental condition
Mouse splenocytes were collected directly from spleen tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
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Mouse thymocyte
Experimental condition
Mouse thymocytes were collected directly from the thymus tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
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Saccharomyces Cerevisiae (50% viability)
Experimental condition
Yeast (Saccharomyces Cerevisiae) cells were cultured in the YPD medium supplemented with several antibiotics overnight. The yeast cells were artificially killed by heating and mixed with healthy cells (50:50). Mixed cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter.
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Size bead
Experimental condition
A high density 3 micron-sized beads were counted with the LUNA-FL to verifiy the performance of the instrument. Beads were counted at the bright field cell counting mode of LUNA-FL. Orange circles represent the automatically-classified objects by LUNA-FL.
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Yeasts in a messy culture
Experimental condition
The number and the viability of yeast cells were measured in a bottle of a Korean rice wine. The wine was diluted at 1:100 with PBS, and the cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter
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ORDERING INFORMATION
Cat # | Product | Qty |
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L20001 | LUNA-FL™ Automated Fluorescence Cell Counter | 1 unit |
L12005 | PhotonSlide™, 50 Slides | 1 box |
L12006 | PhotonSlide™, 500 Slides | 10 boxes |
L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
L12001 | LUNA™ Cell Counting Slides, 50 Slides | 1 box |
L12002 | LUNA™ Cell Counting Slides, 500 Slides | 10 boxes |
L12003 | LUNA™ Cell Counting Slides, 1000 Slides | 20 boxes |
L12011 | LUNA™ Reusable Slide | 1 unit |
L12012 | LUNA™ Reusable Slides | 2 units |
L12014 | LUNA™ Reusable Slide Coverslips | 10 units |
T13001 | Trypan Blue Stain, 0.4% | 2 X 1 mL |
L13002 | Erythrosin B Stain | 2 x 1 mL |
F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
F23002 | Acridine Orange Stain | 2 x 0.5 mL | <
F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
F23202 | Yeast Viability Kit 1 - Propidium Iodide Stain for Yeast (1 x 0.5 mL) - Fluorescein Diacetate Stain (1 x 0.5 mL) - Cell Dilution Buffer (1 x 20 mL) - Fluorescence Signal Enhancer 1 (1 x 0.5 mL) |
1 kit |
F23212 | Cell Dilution Buffer | 5 x 20 mL |
F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
B13101 | LUNA™ Standard Beads | 2 x 1 mL |
F24003 | LUNA-FL™ IQ/OQ Protocol | 1 copy |
P10001 | LUNA™ Printer | 1 unit |
P10002 | LUNA™ Printer Paper - thermal, 700 prints | 3 x 2 rolls |