Fluorescence Cell Counter
The LUNA-FL™ can count in both brightfield and fluorescence mode, making
it our most versatile counter to date.
Advanced imaging software and GFP and RFP fluorescence optics sensitively
detect fluorescence stained cells.
High resolution images are captured and analyzed to detect GFP-positive and -negative cells.
Fluorescence: 30 s = 3 images captured & analyzed;
Brightfield: 10 s = 1 image captured & analyzed
| Cat # | Product | Qty | MSDS | Product Insert |
|---|---|---|---|---|
| B13101 | LUNA™ Standard Beads | 2 x 1 mL |
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| T13001 | Trypan Blue Stain, 0.4% | 2 x 1 mL |
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| L13002 | Erythrosin B Stain | 2 x 1 mL |
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| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
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| F23002 | Acridine Orange Stain | 2 x 0.5 mL |
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| F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
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University Heidelberg
Great performance, would recommend for other researchers!
Easy to use, saves us time and offers good quality. The consultant Dr Eberhardt was also professional and patient to teach us before use.
BGU
Great instrument, I like using it. Really helpful.
The device is easy to use, I mainly use it before cell seeding or to check for my culture's viability and survival. The results are reproducible and the device provides fast results. The accuracy seems to be great as well.
NIH frederick
Highly reproducible.
Very fast and reproducible method for cell counting.
Heidelberg University
Great results.
A very convenient and compact instrument, easy to use. Saving a lot of time for counting cells, counting is extremely accurate.
National Taiwan Ocean University
Easy to use. This cell counter is very easy to use, data storage and output are also very convenient, the only point that can be improved is the use of consumables, I hope they can introduce a reusable slide.
National Institute of Infectious Diseases, Japan
Very easy to use.
I love how easy to use is Luna, I love how fast it counts and probably the feature I love the most is the capability to see previous results and counts. I think we all forget to take notes at some point, but to know the data will always be available immediately is a relief.
University of Federico II di Napoli
Great results!
I love LUNA, easy, fast method to counting cells.
iosBio Ltd
Excellent for fast accurate cell counting!
Brilliant automated cell counter that does both bright field and fluorescent cell counting, allowing for fast and accurate results! The small size means that it can fit anywhere in the lab, and it even has the ability to count GFP transfected/ infected cells which has been very useful for my studies! Would highly recommend!
Stada Ukraine
Easy, speedy and productive.
A perfect tool for your lab. It's very easy in application, highly productive, and really valuable for its money. We use this counter for counting cells during cell culturing and cell passaging. We have had this counter for over 5 years and never have any problems with this equipment.
UZA
Easy in use, quick setup and fast results!
The LUNA-FL is very easy to use and set up. At our biobank, we use it for the quantification of PBMCs after our isolation process in order to make optimal dilutions for long-term storage. Therefore it is crucial that measuring cell count prior to freezing is fast. Counting cells with the LUNA-FL only takes a few minutes all preparation steps included. Great device! Satisfied costumers!
Research
Great instrument!
It is user friendly and operates seamlessly. Provides quality data with high accuracy.
Kumamoto Univ. Department of Cell Signaling and Metabolic Medicine
Good product, easy use.
I mainly use LUNA for cell counting with trypan blue. I have checked if LUNA can precisely detect dead cells, comparing FACS data.
It was perfect, I have no complaints.
Indiana University Genetics Biobank
Easy to use. Accurate results.
I have been using the Coulter counter for most of my professional life but I recently began using the Luna-FL.
Wow! What a difference! The Luna is so easy to use and easy to read. I'm very impressed. Thanks.
Kumamoto Univ. Department of Cell Signaling and Metabolic Medicine
Good product, easy use.
I mainly use LUNA for cell counting with trypan blue. I have checked if LUNA can precisely detect dead cells, comparing FACS data. It was perfect, I have no complaints.
Institut Curie
Friendly tool and fast results.
This tool is efficient and best for counting cells. Friendly to use! I recommand it!
Institut Curie
Fast & efficient tool, a must for every scientist!
Very nice and useful tool for counting cells, efficient and fast as well. The LUNA simplifies the life of every scientists and also the option of counting the dilution to put the right amount of cell concentration is just great. Also the ability to switch from color dye to fluorescent dye is convinient and the precision linked to the count is very good. The quality vs. price is worth it!
Massachusetts General Hospital
Can't imagine my day-to-day work without it.
The LUNA counter is just amazing. Quick cell counting, high sensitivity and repeatability and customizable too. I can use it for estimating the concentration and viability of different cell lines, yeast, and even beads!
Memorial Sloan Kettering Cancer Center
On site demo was great.
On site demo was great. Very detailed instruction on various applications.
NIH
Great results!
Great instrument, no problems. I have been using it everyday since 2018. Mainly for cell counting and viability, quite useful in some proliferation assays.
Institute of Cancer Research
Good machine, no problems so far.
It is a very easy machine to use with reliable results. The slides are fairly expensive but work well, there is an option to have a reusable slide.
Soochow University
Indispensable tool for cell experiment, a right-hand partner.
The precision and accuracy of the equipment are perfect, which saves a lot of time and total cost for our experiment, and provide good cell data. Clear interface, easy to operate, high effectiveness, fast calculation result giving. Best choice for all cell-related experiments which may include cell counting.
Scientist
Great instrument!
Thanks for helping me count my cells! Very easy to use.
University of Pennsylvania
Would recommend to anyone.
The results with this machine are very consistent for both applications I have used it with.
Memorial Sloan Kettering Cancer Center
Great product.
Great product. Fancy fluorescent cell counter. Gives much more accurate results.
Lee Kong Chian School of Medicine
Saves time.
Very useful and saves a lot of time.
CNRS - University of Strasbourg
I could not do without it.
It is a very handy instrument for cell counting. We have been using it for several years and the results obtained are very consistent.
University of Chicago
Easy to use and incredibly helpful.
The LUNA is very helpful and easy to use. It streamlines cell counting allowing us to get more samples done quickly.
St Jude Children's Research Hospital
I have gotten very consistent and accurate nuclei counting results.
LUNA FL cell counter is the best counter for nuclei for single cell genomics. It is simple but very accurate. I am totally satisfied with this product!
Great results, accurate and easy to use!
Great product! LUNA-FL cell counter is very easy to use, quick and accurate. We use it every single day and we have never had a problem with it. It gives you a lot of information about live/dead cells and you can also calculate the volume you need given a cell concentration. I will recommend this product to everybody!
Easy going, fast and reliable.
We bought this because we needed a dual cellcounter. After a demo session with 4 different brands, it became clear that Logos had the best one. It range from stemcells H9-diffs to HEK:MEF/CHO cells.
Great results, definitely recommend!
I have been working with the LUNA cell counter and counting slides for over 1 year and I love how easy and efficient everything is. The LUNA cell counter is a really incredible and useful tool. It allows cell counting to be a straightforward process, with its functionality second to none. Definitely recommend!
Highly recommend to get it.
Very easy to use, quick and reliable results.
Esperovax
So simple but highly repeatable and reliable.
We sometimes have 50-60 samples in a day to count so hemocytometers are not very viable. we needed something that could count yeast and mammalian cells and this does a great job. Also love that it doesn't take up much bench space as that is at a premium in our lab.
pasteur institute
Great result, easy to use and you really save your time.
It is super rapid and easy to use and it is accurate, you will save time and money. I'm really happy with Luna, it gives you the percentage of viable cell, the size of cells and everything else you need.
University of Alabama
Great product and great results.
I have been working with the LUNA-FL cell counter and counting slides for over 3 years. The products are easy to use and very accurate. I've never had a problem and would recommend it to anyone. Great product!
University of Malta
Good results, will be published soon.
This product had made cell counting easy rather than counting it under the microscope as we used to do back in the day. After preparing the sample we easily loaded it into the slide chamber. It also tags which cells are live and dead to be easy for the user to count. since it is done through imaging and focus. The user only has to adjust some minor settings for accurate counting like other instruments. Overall, this is a great product.
Cellarity
Consistent.
The main cell counter that we use before single cell RNAseq. Seems to give consistent results and accurate counts/viabilities.
Easy to use and relatively quick.
Easy, accurate cell counting.
This product has made cell counting such an easy process! After preparing the sample, it is loaded into the slide chamber, inserted into the LUNA-FL, and then counted. Learning to use the equipment was quick, and the default setting has worked well in our lab. Assessing the quality of the count is also easily done through imaging and focus. Overall, this is a great product.
Great tool for T and B cell counting and viability check.
Very easy to use. Very accurate measuring T and B cell viability.
INSERM U955, IMRB Team Sahali
Saves great amount of times if you need to count your cells during the experiment.
Super easy to use, and comes with great explanatory leaflet for quick start. Very accurate results.
Reliable and user friendly.
Fast, reproducible and easy to use. Possibility to save protocols and export data. Helpfull to count GFP cells.
University of Padova
Great results in short time given a proper starting set-up.
I used the LUNA system to estimate cell density and cell viability. In both cases, once the protocol is well defined and the experimental conditions extremely standardized, the system works very efficiently. It is less efficient in a non laboratory conditions.
I have been using this LUNA counter for the last 3 years, it's simple and easy.
The accuracy of cell count is very important for single-cell genomics experiments, in our hands the LUNA is very reliable and repeatable.
Cell counter very convenient to use, accurate and fast.
It's very convenient to use, accurate and fast. The LUNA-FL can distinguish primary cells such as PBMCs, DCs from debris that's why we upgraded from the "classic LUNA" to the LUNA-FL. It's very helpful because we are not limited by cell type or size. In addition, the price is suitable so we're very pleased by that cell counter.
Abivax
An essential instrument for our studies.
We acquired the LUNA-FL in 2017 to replace an old cell counter and we are totally satisfied. We like: -Ease of use -The ability to specifically count PBMCs -The speed and availability of after-sales service -The price very correct.
We are satisfied with the LUNA-FL.
The cell counter LUNA-FL is remarkably easy to use, fast and reliable; with a reactivity of the after-sales service. We are totally satisfied with the LUNA-FL.
Vanderbilt University
Fast and accurate cell viability and counts.
The LUNA-FL's strength is in the viability accuracy, as well as being able to differentiate aggregated cells from single large cells due to the nature of the dual fluorescent dye. I use this machine constantly with cell culture and its speed, ease of use, cost, and small footprint were the major factors in purchasing this over another fluorescent counting option.
Peace of mind progress with cell counting.
We are using the LUNA-FL for counting mostly insect cells during the maintenance and expansion of the cell cultures. It`s operation is very straightforward once the settings are customized according to the cell type. Compared to bright light, fluorescence counting results in much more accurate (up to 10 million cells/ml) and reproducible results. With reusable slide, we don’t have to set focusing every time. It takes a minute or so to get the cell count and viability. Just in case, we always check the tagged cells under bright light and we don’t see any unlabeled or mislabeled cells, independent of cell size or cell clustering.
IU School of Medicine
Amazing customer service, love the instrument.
Our LUNA-FL Cell Counter was having some issues right out of the box. I communicated this to Logos and within the hour, I received a call to help troubleshoot. After attempting to troubleshoot and realizing the attempt was futile, Logos offered to send a replacement instrument within the week. I was extremely impressed by the customer service and I would absolutely buy from Logos again. The company takes pride in their instruments and was professional, courteous and fast to help!
Great for primary cells.
We use the LUNA-FL cell counter for analysis of mouse tissue preparations and primary cell cultures. Our samples include: lung homogenates, bronchoalveolar lavage fluid, T cells culture, etc. Acridine orange/propidium iodide dual staining is particularly beneficial for evaluation of samples contaminated with red blood cells. This approach is fast (analysis conducted immediately after addition of dyes) and more precise than methods utilizing trypan blue staining. Wide detection range and availability of re-usable slides are another advantages of the LUNA-FL. Major disadvantages include: no internal memory, only one USB port - precluding simultaneous use of pendrive (for saving results) and printer, long-lasting saving of results, lack of automatic focusing.
MRC-PPU, University of Dundee
Great cell counter, robust re-usable slides, incredible customer support!
We purchased this cell counter for our busy tissue culture facility. We needed a machine that is easy to use, fast, provides good reproducible results and offers the possibility to save funds on consumables by using re-usable counting slides instead of disposable ones. The LUNA-FL has all these features. The interface is user-friendly, the counter is easy to use with minimum training required and the results are almost immediate, clear and very reproducible. Moreover, users have appreciated the fluorescence function too. It gives a very quick and clear idea of the efficiency of transfection experiments. The possibility to use a re-usable slide with this counter has reduced our expenses towards consumables by an incredible amount. In general, we are very happy with our purchase. A final note has to be made for the incredible support of the customer service: we had a very useful demonstration of the machine, which was left at our facility for a month for trial, without any obligation of purchase. We had an initial big discount for the purchase of two machines, which included two re-usable slides free of charge. When our re-usable slides broke, Logos was upgrading the design and in the meantime we were offered disposable slides free of charge. We have been using the upgraded design for months now, without any problems.
Great but minor improvement needed.
This machine is one of the best .Easy to use and in most cases accurate. However, the application needs minor improvements regarding view on the monitor and speed of counting but overall a great device.
George mason University
Great product with great results.
The product rarely get faulty as all the components are adequately packaged and gives results within few seconds, easy to use as well.
Thanks for the functional product!
This product is great - does what it says it will. Occasionally freezes, but since it works so well I don't care.
Monash University Malaysia
User friendly & fast.
It is user friendly, fast & efficient. It only takes a few seconds to do cell counting and analysis with all info at the main display panel. I like the feature where I can zoom in up to 4x using this equipment.
Highly useful.
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab.
Shenzhen Institutes of Advanced Technology，Chinese Academy of Sciences
Great result & easy to use.
This machine is one of the best .Easy to use and in most cases accurate, rarely get faulty as all the components are adequately packaged and gives results within few seconds.
Try it and determine if it will works fine for you application. Helpful and handy!
It's a compact device useful for cell counting but the camera and software can be much far better in this area of time as we have better resolution camera that can be fancy and much useful with cheaper price. In comparison to other cell counting device, it is comparable. There is a negative point of the design of the slide for cell counting about the depth that can focus better on nuclei counting. It can be faster and much reliable on the stability of the camera focus point for the next usage with the same cell line.
Mitsubishi Corporation Life Sciences
It makes culturing cells easier and faster.
It's very easy to use. If the cell counter had a Japanese version too, it will be better.
An excellent and reliable piece of technology!
The cell counter is very user friendly, and provides reliable/fast cell count and cell viability. The cell counter is useful when you need to count a large number of samples in a short period of time.
Children's National Medical Center
Easy to use, great counting results.
I use it daily for cell counting. It is easy to use and fast to get accurate/reliable results.
Great purchase!
Very practical to use and very precise! In transfection procedures it's very helpful and speeds up our experiments a lot. I'm loving it!
MIVEGEC, IRD
Good product, quick and efficient!
It is very useful to count quickly, in comparison with the cell of Malassez. It also allows you to have the number of dead cells and a living / dead cell ration. I highly recommend this product!
Super, I use it every day to count my cells.
It is a really nice instrument to measure the amount of cells. It gives really quickly results.
University of Geneva
Easy to use to obtain robust and reproducible results.
The LUNA-FL Dual Flourescence Cell Counter is easy to adjust and to use to obtain reproducible results. The high-resolution quality of the bright field and fluorescent images allows efficient and quick analysis of the cell count and cell viability. Once the parameters are set it is possible to obtain a robust read-out independent of the cell morphology. We use the LUNA-FL Dual Fluorescence Cell counter every day and it facilitates fast and user-friendly application. It is possible to save the bright field and fluorescent images on a portable drive for further quantification.
A reliable and nice cell counting tool.
We have been use the LUNA-FL Cell Counter already for years and its ease of use and reliability make us happy users.
University of Michigan
Easy to use, uses small space, reliable, no warm-up waiting.
Easy to use. Once you turn it on, it is ready to use, so no warm-up waiting. It counts cells pretty fast, in less than a minute. One problem is that for cells that strongly aggregate or are clumpy, readings are not accurate.
Fast and accurate, the product to have!
The LUNA-FL is easy to use and it gives reproducible results between cell counts. Moreover, it saves me a lot of time as each count is very fast and accurate! It is a good product to have in a cell lab.
Parexel International
Great software interface and easy to use!
Setup and software interface is easy to use. Very sturdy product.
Handle samples quickly.
Works easily. We like this labelling and try to do work with 3D culture.
Flow Core
Excellent results, a key player in our cell counting pipeline.
Extremely reliable and useful. The ability to look at live/dead using the AO/PI stain gives the opportunity to ensure the correct cell concentration is being measured.
Great value for money.
For our experiments, we use a lot of fluorescence cell counting. This instrument definitely helped us reduce the time we spend in achieving the results.
University of Al-Qadisiyah
It made it easy to to be in the lab.
It made it easy to be in the lab with good experience and high quality, reproducible results.
it is a good instrument which will be very helpful for cell proliferation assays.
I am satisfied with this equipment. it is very easy to use.
Genome&Company
Easy, fast and accurate machine!
Until this machine was used, the cells were directly counted and observed under a microscope. However, after using the LUNA-FL Dual Fluorescence Cell Counter, we were able to save about half the time and get accurate results. Very easy to use which is also an advantage of this machine. I recommend to everyone!
Great cell counting machine.
The LUNA-FL works very well for counting cells using trypan blue. It is able to recognize live and dead cells in many different types of cells.
Hebrew University of Jerusalem
A very versatile, easy to use cell counter with many useful functions.
It is a very nifty fluorescent cell counter. Very easy to use and with high reproducibility. Many useful functions for gating, cell size measurement and for counting yeast. I have used other counters but this one surpasses them all. We are very happy that we introduced it into our lab. Highly recommended!
Ensures scRNA-Seq Success.
This instrument has been indispensable for our single cell RNA-Seq sample prep process. Most of our samples are primary cells that have a high potential for debris and cell aggregates, which make traditional hemocytometer/trypan counting difficult. Dual fluorescence counting has greatly improved our accuracy and reliability. We also love being able to digitally archive the images/count reports for later reference. The instrument controls are intuitive and allow for manual focusing of the cell images. Our service rep has been very quick to address our after-sales needs. The LUNA-FL is the most affordable instrument that meets our needs (and often exceeds them!). Would recommend.
DUKE INTERNAL MEDICINE ONCOLOGY
Accurate and easy to use.
We are a research lab that does in vitro and in vivo immunological studies. Viability and accurate cell counts are extremely important to our studies. The LUNA-FL is extremely user friendly and easy to switch from brightfield to fluorescence to GFP. The option to use a resusable slide also makes this instrument cost effective when only counting one sample. It takes up very little bench space and fits in our small tissue culture room where space is a premium. The ability to demo the LUNA instruments side by side for as long as we needed was a must for us and Logos allowed us the time and support to test all counting methods completely.
Fast, rapid, reliable counting with AO/PI.
From 10 ml whole blood it is estimated to obtain roughly 10 million PBMCs. When counting with the LUNA-FL with the AO/PI stain, we indeed see total cell counts approximating what we expected from what is known. This system in combo with this dye mix offers a fast, reliable solution to count PBMCs.
GMU
Easy to use, saves time and is very efficient.
The equipment is very reliable with great accuracy and precision. It is user friendly and it is easy to use. In my former lab, we counted cells via microscope using the counting chamber with which it was very difficult to identify dead cells. With this equipment my job has been made easy.
Easy to use!
The cell counter is easy to use, great for teaching, and very accurate most of the time. Use the bright field setting and the fluorescent setting most frequently and am satisfied with both.
Covaris
Very accurate and easy to use.
The LUNA-FL Dual Fluorescence Cell Counter is very fast and easy to use. Our lab utilizes this instrument to count multiple cell types of different sizes; I much prefer it to a hemocytometer. The LUNA-FL is small and easily fits on our bench top. The counting and viability data is accurate and reproducible.
No need to look for any other counter.
No need to look anywhere else for a cell counter. This is used by every group. It's very easy to use, and the ability to save count reports on different drives makes life so much easier than hand-recording everything.
Usc
Great results, use it daily.
This product is great I use it multiple times a day and it’s always consistent and useful. It’s my favorite cell counter in the lab! Highly recommend!
Easy to use product.
Used to count living/dead cells after tissue digestion. Great service and easy to use product!
NIH
Reliable, easy-to-use, compact and accurate - highly recommend!
Our lab has been using the LUNA-FL Dual Fluorescence Cell Counter for almost a year now and we have nothing but great things to say about it. Operating it is extremely straightforward with its simple and user-friendly interface. In addition to providing quick and accurate results, it includes many customizable features for all of your cell counting needs.
Reliable and easy to use.
This cell counter is extremely easy to use and has provided quick and accurate results on a consistent basis. It saves a lot of time and doesn't take up much lab space. I would highly recommend this product to anyone looking for an accurate cell counter.
University Hospital Zurich
Simplifies the procedure of preparing single-cell suspension.
Easy-to-use and user-friendly interface. A must have tool for labs that have a lot of cell culture work and do a lot of viability assays. The customer service was amazing - gave us two demo tests.
Thank you for this product, it is really helping me.
It is not easy to count neutrophils, and it takes so long time do it manually. When I am using the LUNA-FL with PhotonSlide, it is a very easy, fast and precise count.
Antibody Solutions
Best cell counting instrument on the market!
We use our LUNA-FL Dual Fluorescence Cell Counter every day for our flow assays and other duties. The first thing I noticed about the LUNA-FL is that it is very easy to use. Sometimes we are counting multiple cell types and the LUNA-FL is quick and very accurate. The LUNA cell counting slides we use are double sided, which helps to reduce waste, and they are of high quality. Overall this product has no flaws in my opinion.
Great results and takes no time at all to use!
The Cell Counter is effective for allowing us to make better use of our time instead of using manual counting methods like the hemacytometer. There is some variability to the results, however they are for the most part consistent. The Cell Counter is intuitive in its interface and easy to operate with minimal training. One should be mindful of dilutions. So far we've had no problem (sourced to the equipment) and have had many instances of its reliable use.
Charité Comprehensive Cancer Center
Good results, easy to use.
The LUNA-FL is a very compact, lightweight device with an intuitive user interface that only requires minimal training. We use it every day for live/dead cell counting and are satisfied with the results. Logos offers disposable slides for high-throughput but also reusable ones for smaller labs (with a little effort you can also reuse the disposable ones but you didn't hear that from me). Preparation of slides with AO/PI takes about 1 minute.
Great results, saves time!
The cell counter is very effective and saves time instead of using a hemocytometer.
Max Delbruck Center
Fast and easy to use!
Fast and useful cell counter! LUNA-FL is an optimal automated cell counter, easy to use and reliable. I am really happy with this product and I would absolutely recommend it. Save your time and make differences to your experiments.
Precise, compact, intuitive!
We use LUNA-FL Cell Counter to count different cell lines, allowing us to save valuable time, and to have precise and reproducible results. Easy to use, with minimal training. Compact size, in fact it can be put in a drawer. Fast reading, with a detailed outcomes.
Hospital S. Pau
Very fast and complete solution.
We use it as a cell counter. It has many functions, it's very fast and takes up very little space. It uses very little sample and gives a lot of information.
Great results.
It was an amazing cell counter. It helped us a lot and saved a lot of time.
Organization: Turku Bioscience
Enjoy the joy of counting cellsEnjoy the joy of counting cells.
Among the best cell counters I have used so far! Easy and quick to use. One can export the high-quality images of cell counting field for references. The cell counting is quite precise.
Easy to use and good results.
It's very fast and takes up very little space. I'm really happy with this product and I would absolutely recommend it. It is easy to count PBMC.
High-end vaccine biologics
A simple and time-saving cell counter.
It is very convenient for counting cells and save time. You can see the cells on the screen and recalculate the cells with the "tag" key. The results can also be output as a PDF file for records and reports.
Excellent device for quick and accurate cell-counts.
We use LUNA-FL for counting diatom cells routinely in our lab. Its extremely convenient and fast for taking cell counts which are reliable and robust. We have been using the LUNA-FL for about 3 years and have had no problems with this device. Highly recommend it for routine labwork.
Latvian Institute of Organic synthesis
From very simple use to rapid analysis.
LUNA-FL has helped us in day-to-day work and provides even more opportunities than hoped. Dual fluorescence optics help us obtain more accurate and reliable results, representative images, and high quality on-board analysis. Histogram data with cell size distribution and cell-cluster graphs are very important because we are using various cell lines and primary cells as well. Great value for money!
Nice device for saving time and sanity.
Easy to use and very useful for determination of transfection efficiency using GFP transfection assays.
Western Illinois University
Great instrument.
The instrument is reliable and does counting perfectly.
Accurate results, easy to use, great sales and follow up support.
We purchased the Logos Biosystems LUNA-FL cell counter to quantitate human T lymphocytes and other cell types. This was early 2020, so most everything was shut down due to the pandemic, but we needed a good cell counter for SARS-CoV-2 research. To test if the instrument would meet our needs, Logos shipped us a demo to try out. The machine is very easy to use, accurate, and provides lots of supplemental data beyond just cell number. The LUNA-FL cost a bit more than some comparable counters, but less than others. The sales support and flexibility was what really stood out and sold our lab. The disposable slides can get expensive when performing many counts, but that's a common refrain for most counters. To save on the costs of consumables, I recommend buying straight from Logos; reusable slides also exist. All-in-all, this instrument, and the support behind it, have met our lab's needs well. We're happy and without complaints.
RxCelerate
Great bit of kit.
The LUNA-FL cell counter is a great bit of kit, it has really saved us a lot of time in the lab. It's compact, space saving and intuitive. It's great having the flexibility of having both brightfield and fluorescence cell counting in one machine.
Great. Easy to use.
Imperial College London
I love the LUNA-FL counter. It's so easy to use and it's fast - which is important for my applications when I can't have cells on ice for too long. I love the fact that there are multiple counting modes (dual fluorescence or brightfield) and you can set it up to use your custom stains. Getting practically instant cell counts with viability and dilution calculations done automatically is excellent. The other huge advantage over other automated counting systems is that you can visualize your cells on the screen to ensure they're healthy and spot any anomalies, which you can't do with cuvette based counters for example. It's also great that it stores all the data and can export it to USB, including cell images too. Very easy to calibrate, also.
Great device, user-friendly and time saving.
We use this cell counter to count THP1 cell lines, cultured mesenchymal stromal cells and, thanks to its fluorescence, stromal cells from stromal vascular fractions. The results are more consistent than with a classic hemocytometer. The device is really easy to use and displays the picture of the counting, allowing you to check what counted as a cell or not. You even have a graph with average sizes of the cells. Logos is a company close to their clients and they are very helpful. To conclude: No regret, and time saved!
Taiwan Color Optics
Great product specialists in Taiwan.
Thanks for good product specialists in Taiwan. They helped solve our counting problems.
Great! Useful instrument to count cells and test viability.
Easy to use. A little bit expensive.
Memorial Sloan Kettering Cancer Center
100% recommended!
This is my very first time using it for cell counting. It is very easy to use, effective, time-saving and reliable.
Fast and sparing with my cells.
Very convenient cell counter. Does not need much space on the bench and results are reliable (checked in comparison to FACS analysis. It is great that only a small amount of cell suspension is used to get quick results!
Medigen
Worth buying!
LUNA-FL Dual Flurescenece cell counter elevate the efficiency of counting cell and increase our trust to machine counting system.
Really handy, using it on a daily basis.
Good stuff, easy to use and compact.
LBRI
Easiest cell counter to use on the market.
By far the easiest cell counter to use on the market. It clearly tags live and dead cells so the user can check the accuracy of the count. The user does not have to make a ton of adjustments for accurate counting like other instruments.
Children's National Medical Center
The LUNA-FL is one of my favorite machines in our lab. It lets me acquire cell counts so quickly so I can go on with my bench work. Not only are we able to count but we can look at other statistics like viability and average cell size with just the push of a button. The best part about this counter is the accuracy. I am able to reliably count every cell line that I put in, and am able to do it again the next day. I'm so glad we have this system in our lab because counting with trypan blue would take much longer! Overall, I would recommend this product to another lab that is doing a lot of work with cell lines in order to get a quick and accurate count.
Roger Williams Medical Center
The LUNA-FL is an extremely easy device to use and very user friendly. With many different modes available, it is certainly a useful tool in the lab. Our service rep is extremely responsive and helpful to our needs and questions. Highly recommend!
Weill Cornell Medicine / New York-Presbyterian Hospital
The LUNA-FL consistently produces fast and reliable results. The counts and viabilities provided by this instrument are consistent with values provided by flow cytometry. This product is remarkably easy to operate, care for, and maintain. In comparison to similar products on the market, the LUNA-FL is exceptional for the low cost.
Medical University of South Carolina
Our lab was interested in an automated cell counter because we needed something reproducible enough to compare between groups and to use cell count as a quick approach for normalization. … The LUNA-FL fit our needs the most, and the price was close to our budget. So far, it has been used for many purposes in our lab, including the original assay that we were trying to develop and other applications including quick live/dead information, GFP expression, etc.
Korea University Medical School
I would recommend it simply because it can count a heterogeneous sample obtained from mouse brain. I was surprised by the accuracy of this tiny instrument.
NIH, NHGRI
It's very convenient to use and very accurate. Especially in the transfection procedures, it's very helpful. Overall, we're very pleased.
NIH, NIAID
It works really well. It's easy. It's accurate and fast. I especially like the dual fluorescence and being able to focus on the green and red cells.
Brain Cancer Cell-derived Exosomes Protect Scopolamine-Induced Death of SH-SY5Y Neuron Cells.
2021. Lee M. International Journal of Health Sciences and Research 10.36838/v3i1.8.
The Type and Source of Reactive Oxygen Species Influences the Outcome of Oxidative Stress in Cultured Cells.
2021. Goffart S, Tikkanen P, Michell C, Wilson T, Pohjoismäki JLOLO. Cells 10(5):1075.
Immune Memory in Mild COVID-19 Patients and Unexposed Donors Reveals Persistent T Cell Responses After SARS-CoV-2 Infection.
2021. Ansari A, Arya R, Sachan S, Jha SN, Kalia A, Lall A, Sette A, Grifoni A, Weiskopf D, Coshic P, Sharma A, Gupta N. Frontiers in Immunology 12:636768.
The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System.
2021. Fujibayashi K, Mima J. Frontiers in Cell and Developmental Biology 9:628910.
Flow cytometric quantification of apoptotic and proliferating cells applying an improved method for dissociation of spheroids.
2021. Metzger W, Rösch B, Sossong D, Bubel M, Pohlemann T. Cell Biology International 10.1002/cbin.11618.
Paraphysoderma sedebokerense Infection in Three Economically Valuable Microalgae: Host Preference Correlates with Parasite Fitness.
2021. Alors D, Boussiba S, Zarka A. Journal of Fungi 7(2):100.
Role of TLR4 in persistent Leptospira interrogans infection: a comparative in vivo study in mice.
2021. Nair N, Guedes MS, Hajjar AM, Werts C, Gomes-Solecki M. Frontiers in Immunology 11:572999.
Protective effects of luteolin on the venous endothelium.
2021. Assunção HCR, Cruz YMC, Bertolino JS, Garcia RCT, Fernandes L. Molecular and Cellular Biochemistry 476(4):1849–1859.
The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System.
2021. Fujibayashi K, Mima J. Frontiers in Cell and Developmental Biology 9:628910.
Exposure-related, global alterations in innate and adaptive immunity; a consideration for re-use of non-human primates in research.
2021. Bates FA, Duncan EH, Simmons M, Robinson T, Samineni S, Strbo N, Villasante E, Bergmann-Leitner E, Wijayalath W. PeerJ 9:e10955.
Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
2021. Mashiko T, Kanayama K, Saito N, Shirado T, Asahi R, Mori M, Yoshimura K. Cells 10(3):560
High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis.
2021. Gaurav R, Mikuls TR, Thiele GM, Nelson AJ, Niu M, Guda C, Eudy JD, Barry AE, Wyatt TA, Romberger DJ, Duryee MJ, England BR, Poole JA. PLoS One 16(2):e0240707.
Inhibiting calpain 1 and 2 in cyclin G associated kinase–knockout mice mitigates podocyte injury.
2020. Tian X, Inoue K, Zhang Y, Wang Y, Sperati CJ, Pedigo CE, Zhao T, Yan M, Groener M, Moledina DG, Ebenezer K, Li W, Zhang Z, Liebermann DA, Greene L, Greer P, Parikh CR, Ishibe S. JCI Insight 5(22):e142740.
Multi-Omics Resolves a Sharp Disease-State Shift between Mild and Moderate COVID-19.
2020. Su Y, Chen D, Yuan D, Lausted C, Choi J, Dai CL, Voillet V, Duvvuri VR, Scherler K, Troisch P, Baloni P, Qin G, Smith B, Kornilov SA, Rostomily C, Xu A, Li J, Dong S, Rothchild A, Zhou J, Murray K, Edmark R, Hong S, Heath JE, Earls J, Zhang R, Xie J, Li S, Roper R, Jones L, Zhou Y, Rowen L, Liu R, Mackay S, O'Mahony DS, Dale CR, Wallick JA, Algren HA, Zager MA; ISB-Swedish COVID19 Biobanking Unit, Wei W, Price ND, Huang S, Subramanian N, Wang K, Magis AT, Hadlock JJ, Hood L, Aderem A, Bluestone JA, Lanier LL, Greenberg PD, Gottardo R, Davis MM, Goldman JD, Heath JR. Cell 183(6):1479-1495, e20.
Baculovirus-vectored precision delivery of large DNA cargoes in human genomes.
2020. Aulicino F, Pelosse M, Toelzer C, Capin J, Meysami P, Dillingham MS, Schaffitzel C, Berger I. Biorxiv 2020.08.17.253898.
Quantitative Assays Reveal Cell Fusion at Minimal Levels of SARS-CoV-2 Spike Protein and Fusion-from-Without.
2020. Theuerkauf SA, Michels A, Riechert V, Maier TJ, Flory E, Cichutek K, Buchholz CJ. Biorxiv 2020.10.15.340604.
Identification of genomic enhancers through spatial integration of single‐cell transcriptomics and epigenomics.
2020. Bravo González-Blas C, Quan XJ, Duran-Romaña R, Taskiran II, Koldere D, Davie K, Christiaens V, Makhzami S, Hulselmans G, de Waegeneer M, Mauduit D, Poovathingal S, Aibar S, Aerts S. Molecular Systems Biology 16(5):e9438.
Safety and Tolerability of Stromal Vascular Fraction Combined with β-Tricalcium Phosphate in Posterior Lumbar Interbody Fusion: Phase I Clinical Trial.
2020. Choi UY, Kim KT, Kim KG, Lim SH, Kim YJ, Sohn S, Sheen SH, Heo CY, Han I. Cells 9(10):2250.
Real-Time Quantitative Reverse Transcription PCR for Detection of Opioid Receptors in Immune Cells.
2020. Celik MÖ, Labuz D, Machelska H. Methods in molecular biology (Clifton, N.J.) 2201:83–95.
Selection of microRNAs in extracellular vesicles for diagnosis of malignant pleural mesothelioma by in vitro analysis.
2020. Jotatsu T, Izumi H, Morimoto Y, Yatera K. Oncology Reports 44(5):2198–2210.
Establishment of an In Vitro Model of Persistent Chicken Anemia Virus Infection.
2020. Van Dong H, Tran GTH, Trinh DQ, Takeda Y, Ogawa H, Imai K. Pathogens (Basel, Switzerland) 9(10):842.
High‐Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment.
2020. Enríquez A, Libring S, Field TC, Jimenez J, Lee T, Park H, Satoski D, Wendt MK, Calve S, Tepole AB, Solorio L, Lee H. Advanced Functional Materials 10.1002/adfm.202005021.
Safety and Tolerability of Stromal Vascular Fraction Combined with β-Tricalcium Phosphate in Posterior Lumbar Interbody Fusion: Phase I Clinical Trial.
2020. Choi UY, Kim KT, Kim KG, Lim SH, Kim YJ, Sohn S, Sheen SH, Heo CY, Han I. Cells 9(10):2250.
Chemically modified mRNA nucleofection of primary human T cells.
2020. Thuille N, Sajinovic T, Siegmund K, Baier G. Journal of Immunological Methods 112878.
Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging.
2020. Ma S, Sun S, Geng L, Song M, Wang W, Ye Y, Ji Q, Zou Z, Wang S, He X, Li W, Esteban CR, Long X, Guo G, Chan P, Zhou Q, Belmonte JCI, Zhang W, Qu J, Liu GH. Cell 180(5):984–1001.e22.
Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior.
2020. Shie MY, Lee JJ, Ho CC, Yen SY, Ng HY, Chen YW. Polymers (Basel) 12(9):E1930.
Single-cell transcriptomic atlas of primate cardiopulmonary aging.
2020. Ma S, Sun S, Li J, Fan Y, Qu J, Sun L, Wang S, Zhang Y, Yang S, Liu Z, Wu Z, Zhang S, Wang Q, Zheng A, Duo S, Yu Y, Belmonte JCI, Chan P, Zhou Q, Song M, Zhang W, Liu GH. Cell Research 10.1038/s41422-020-00412-6.
Quantitative Detection of Plasmodium falciparum Using, LUNA-FL, A Fluorescent Cell Counter.
2020. Hashimoto M, Yokota K, Kajimoto K, Matsumoto M, Tatsumi A, Yamamoto K, Hyodo T, Matsushita K, Minakawa N, Mita T, Oka H, Kataoka M. Microorganisms 4;8(9):E1356.
Post-copulatory genetic matchmaking: HLA-dependent effects of cervical mucus on human sperm function.
2020. Jokiniemi A, Magris M, Ritari J, Kuusipalo L, Lundgren T, Partanen J, Kekäläinen J. Biological Sciences 287(1933):20201682.
Human Mesenchymal Stem Cell Hydrogen Sulfide Production Critically Impacts the Release of Other Paracrine Mediators After Injury.
2020. Markel TA, Drucker NA, Jensen AR, Olson KR. The Journal of Surgical Research 254:75-82.
Analytical comparison of structural changes of plastic cell-based therapeutic drug storage containers when exposed to cryogenic environments.
2020. Park JY, Lee DM, Lee J, Lee SR, Yoon GS. Journal of the Korea Society of Die & Mold Engineering 14(2):1-6.
High-Content Immunophenotyping and Hierarchical Clustering Reveal Sources of Heterogeneity and New Surface Markers of Human Blood Monocyte Subsets.
2020. Hoffmann J, Fišer K, Liebetrau C, Staubach N, Kost D, Voss S, Heiden AZ, Dörr O, Lipps C, Nef HM, Möllmann H, Hamm CW, Keller T, Troidl C. Thrombosis and Haemostasis 120(1):141-155.
Single-cell transcriptome analysis of the Akimba mouse retina reveals cell-type-specific insights into the pathobiology of diabetic retinopathy.
2020. Van Hove I, De Groef L, Boeckx B, Modave E, Hu TT, Beets K, Etienne I, Van Bergen T, Lambrechts D, Moons L, Feyen JHM, Porcu M. Diabetologia doi: 10.1007/s00125-020-05218-0.
Similarities and Differences in the Effects of Toxic Concentrations of Cadmium and Chromium on the Structure and Functions of Thylakoid Membranes in Chlorella variabilis.
2020. Zsiros O, Nagy G, Patai R, Solymosi K, Gasser U, Polgár TF, Garab G, Kovács L, Hörcsik ZT. Frontiers in Plant Science 11:1006.
Vascular occlusion by neutrophil extracellular traps in COVID-19.
2020. Leppkes M, Knopf J, Naschberger E, Lindemann A, Singh J, Herrmann I, Stürzl M, Staats L, Mahajan A, Schauer C, Kremer AN, Völkl S, Amann K, Evert K, Falkeis C, Wehrfritz A, Rieker RJ, Hartmann A, Kremer AE, Neurath MF, Herrmann M. EBioMedicine 58:102925.
Optimal Initial Cell Density that Yields the Highest Number of Primary Synovial Mesenchymal Stem Cells in a Clinical Setting.
2020. Horiuchi K, Mizuno M, Katano H, Endo K, Ozeki N, Tsuji K, Koga H, Sekiya I. Stem Cell & Developmental Cell Biology doi: 10.21203/rs.3.rs-28311/v1.
Photosynthetic characterization of two Nannochloropsis species and its relevance to outdoor cultivation.
2020. Vonshak A, Novoplansky N, Benavides AM, Torzillo G, Beardall J, Palacios YM. Journal of Applied Phycology https://doi.org/10.1007/s10811-019-01985-5.
Increased O-GlcNAcylation of c-Myc Promotes Pre-B Cell Proliferation.
2020. Lee DH, Kwon NE, Lee WJ, Lee MS, Kim DJ, Kim JH, Park SK. Cells 9(1):158.
Inhibition of the PI3K-AKT-mTOR pathway suppresses the adipocyte-mediated proliferation and migration of breast cancer cells.
2020. Park JY, Kang SE, Ahn KS, Um JY, Yang WM, Yun M, Lee SG. Journal of Cancer 2020;11(9):2552‐2559.
Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content.
2020. Fukumori R, Oba M, Izumi K, Otsuka M, Suzuki K, Gondaira S, Higuchi H, Oikawa S. Journal of Dairy Science 103(4):3656‐3667.
Curvature-sensitive trans-assembly of human Atg8-family proteins in autophagy-related membrane tethering.
2020. Taniguchi S, Toyoshima M, Takamatsu T, Mima J. Protein Science : A Publication of the Protein Society 10.1002/pro.3828.
A Comparison of Ovine Facial and Limb Muscle as a Primary Cell Source for Engineered Skeletal Muscle.
2020. Rodriguez BL, Nguyen MH, Armstrong RE, Vega-Soto EE, Polkowski PM, Larkin LM. Tissue Engineering Part A 26(3-4):167‐177.
Uptake and excretion dynamics of gold nanoparticles in cancer cells and fibroblasts.
2020. Ivošev V, Sánchez GJ, Stefancikova L, Haidar DA, González Vargas CR, Yang X, Bazzi R, Porcel E, Roux S, Lacombe S. Nanotechnology 31(13):135102.
Cloning and functional testing of rhesus macaque (Macaca mulatta) IL‐9 and IL‐33.
2020. Sarkar S, Hessell AJ, Haigwood NL, Kobie JJ. Journal of Medical Primatology 49(3):144‐152.
An optimized workflow for single-cell transcriptomics and repertoire profiling of purified lymphocytes from clinical samples.
2020. Hanamsagar R, Reizis T, Chamberlain M, Marcus R, Nestle FO, de Rinaldis E, Savova V. Scientific Reports 10(1):2219.
Characterization and mutagenesis of Chinese hamster ovary cells endogenous retroviruses to inactivate viral particle release.
2020. Duroy PO, Bosshard S, Schmid-Siegert E, Neuenschwander S, Arib G, Lemercier P, Masternak J, Roesch L, Buron F, Girod PA, Xenarios I, Mermod N. Biotechnology and Bioengineering 117(2):466‐485.
NeuroCore formation during differentiation of neurospheres of mouse embryonic neural stem cells.
2020. Lee JH, Shaker MR, Lee E, Lee B, Sun W. Stem Cell Research 43:101691.
Metabolomic analysis of serum reveals the potential effective ingredients and pathways of Danggui Buxue Tang in promoting erythropoiesis.
2020. Wang X, Bei H, Du R, Chen Q, Wu F, Chen J, Bo H. Complementary Therapies in Medicine 48:102247.
Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy.
2019. Wei J1, Long L, Zheng W, Dhungana Y, Lim S1, Guy C, Wang Y, Wang YD, Qian C, Xu B, Kc A, Saravia J, Huang H, Yu J, Doench JG, Geiger TL, Chi H. Nature, DOI: 10.1038/s41586-019-1821-z. [Epub ahead of print]
Time-lapse image analysis for whole colony growth curves and daily distribution of the cell number per colony during the expansion of mesenchymal stem cells.
2019. Mizuno M, Katano H, Shimozaki Y, Sanami S, Ozeki N, Koga H, Sekiya I. Scientific Reports 9: 16835.
Furin-mediated intracellular self-assembly of olsalazine nanoparticles for enhanced magnetic resonance imaging and tumour therapy.
2019. Yuan Y, Zhang J, Qi X, Li S, Liu G, Siddhanta S, Barman I, Song X, McMahon MT, Bulte JWM. Nature Materials 18: 1376–1383.
Characterization and mutagenesis of Chinese hamster ovary cells endogenous retroviruses to inactivate viral particle release.
2019. Duroy PO, Bosshard S, Schmid-Siegert E, Neuenschwander S, Arib G, Lemercier P, Masternak J, Roesch L, Buron F, Girod PA, Xenarios I, Mermod N. Biotechnology and Bioengineering, DOI: 10.1002/bit.27200. [Epub ahead of print]
Differentiation of baboon (Papio anubis) induced-pluripotent stem cells into enucleated red blood cells.
2019. Olivier EN, Wang K, Grossman J, Mahmud N, Bouhassira EE. Cells 8(10): 1282.
PINK1/Parkin influences cell cycle by sequestering TBK1 at damaged mitochondria, inhibiting mitosis.
2019. Sarraf SA, Sideris DP, Giagtzoglou N, Ni L, Kankel MW, Sen A, Bochicchio LE, Huang CH, Nussenzweig SC, Worley SH, Morton PD, Artavanis-Tsakonas S, Youle RJ, Pickrell AM. Cell Reports 29(1): 225-235.
Engineered fibrillar fibronectin networks as three‐dimensional tissue scaffolds.
2019. Jordahl S, Solorio L, Neale DB, McDermott S, Jordahl JH, Fox A, Dunlay C, Xiao A, Brown M, Wicha M, Luker GD, Lahann J. Advanced Materials 1904580.
Mitigation of T-cell dependent immunogenicity by reengineering factor VIIa analogue.
2019. Jankowski W, McGill J, Lagassé HAD, Surov S, Bembridge G, Bunce C, Cloake E, Fogg MH, Jankowska KI, Khan A, Marcotrigiano J, Ovanesov MV, Sauna ZE. Blood Advances 3(17): 2668-2678.
A comparison of ovine facial and limb muscle as a primary cell source for engineered skeletal muscle.
2019. Rodriguez BL, Nguyen MH, Armstrong RE, Vega-Soto EE, Polkowski PM, Larkin LM. Tissue Engineering Part A doi.org/10.1089/ten.TEA.2019.0087.
Roles of interleukin-11 during acute bacterial pneumonia.
2019. Traber KE, Dimbo EL, Symer EM, Korkmaz FT, Jones MR, Mizgerd JP, Quinton LJ. PLoS One 14(8): e0221029.
DLC1 deficiency and YAP signaling drive endothelial cell contact inhibition of growth and tumorigenesis.
2019. Ritchey L, Ha T, Otsuka A, Kabashima K, Wang D, Wang Y, Lowy DR, Tosato G. Oncogene 10.1038/s41388-019-0944-x.
Diffusion-weighted MRI and 18F-FDG PET correlation with immunity in early radiotherapy response in BNL hepatocellular carcinoma mouse model: timeline validation
2019. Chung YH, Yu CF, Chiu SC, Chiu H, Hsu ST, Wu CR, Yang CL, Hong JH, Yen TC, Chen FH. European Journal of Nuclear Medicine and Molecular Imaging: 1-12.
A microwell array platform to print and measure biomolecules produced by single cells
2019. Abali F, Broekmaat J, Tibbe A, Schasfoort RBM, Zeunea L, Terstappen LWMM. Lab on a Chip 19: 1850-1859.
Thrombin preconditioning of extracellular vesicles derived from mesenchymal stem cells accelerates cutaneous wound healing by boosting their biogenesis and enriching cargo content.
2019. Sung DK, Chang YS, Sung SI, Ahn SY, Park WS. Journal of Clinical Medicine 8(4): 533.
Homotypic and heterotypic trans-assembly of human Rab-family small GTPases in reconstituted membrane tethering.
2019. Segawa K, Tamura N, Mima J. Journal of Biological Chemistry 294: 7722-7739.
Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human induced pluripotent stem cells.
2019. Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Mizuguchi H, Sato Y. Scientific Reports 9(1): 3630.
Chimeric antigen receptor costimulation domains modulate human regulatory T cell function.
2019. Boroughs AC, Larson RC, Choi BD, Bouffard AA, Riley LS, Schiferle E, Kulkarni AS, Cetrulo CL, Ting D, Blazar BR, Demehri S, Maus MV. JCI Insight 4(8): e126194.
Exploiting evolutionary herding to control drug resistance in cancer.
2019. Acar A, Nichol D, Fernandez-Mateos J, Cresswell GD, Barozzi I, Hong SP, Spiteri I, Stubbs M, Burke R, Stewart A, Vlachogiannis G, Maley CC, Magnani L, Valeri N, Banerji U, Sottoriva A. bioRxiv 566950.
SOX-6, 9-transfected adipose stem cells to treat surgically-induced osteoarthritis in goats.
2019. Ko JY, Lee J, Lee J, Ryu YH, Im GI. Tissue Engineering Part A.
Initial assessment of variability of responses to toxicants in donor-specific endothelial colony forming cells.
2018. Filonov D, Tice R, Luo R, Grotegut C, Van Kanegan MJ, Ludlow JW, Il'yasova D, Kinev A. Frontiers in Public Health 6: 369.
Predicted glycosyltransferases promote development and prevent spurious cell clumping in the choanoflagellate S. rosetta.
2018. Wetzel LA, Levin TC, Hulett RE, Chan D, King GA, Aldayafleh R, Booth DS, Sigg MA, King N. ELife 7: e41482.
Single-cell transcriptomics reveals multi-step adaptations to endocrine therapy.
2018. Hong SP, Chan TE, Lombardo Y, Corleone G, Rotmensz N, Pruneri G, McEwen KR, Coombes RC, Ali S, Barozzil I, Magnani L. Nature Communications – under consideration.
CD19-directed CAR T-cell (CTL019) Product Viability and Clinical Outcomes in Non-Hodgkin Lymphomas and B-cell acute lymphoblastic leukemia.
2018. Chong EA, Levine BL, Grupp SA, Davis M, Siegel DL, Maude SL, Gladney WL, Frey NV, Porter DL, June CH, Schuster SJ. Blood 132 (Suppl 1): 197.
Zooming in on cryopreservation of hiPSCs and neural derivatives: A dual-center study using adherent vitrification.
2018. Kaindl J, Meiser I, Majer J, Sommer A, Krach F, Katsen-Globa A, Winkler J, Zimmermann H, Neubauer JC, Winner B. Stem Cells Translational Medicine 8(3): 247-259.
Blocking nuclear export of HSPA8 after heat shock stress severely alters cell survival.
2018. Wang F, Bonam SR, Schall N, Kuhn L, Hammann P, Chaloin O, Madinier JB, Briand JP, Page N, Muller S. Scientific Reports 8(1): 16820.
Development of new 3D human ex vivo models to study sebaceous gland lipid metabolism and modulations.
2018. de Bengy AF, Forraz N, Danoux L, Berthelemy N, Cadau S, Degoul O, Andre V, Pain S, McGuckin C. Cell Proliferation 52(1): e12524.
Surface modification of macrophages with nucleic acid aptamers for enhancing the immune response against tumor cells.
2018. Sugimoto S, Iwasaki Y. Bioconjugate Chemistry 29(12): 4160-4167.
Association of the human bocavirus with tonsil squamous cell carcinomas.
2018. Höpken M, Förster I, Maune S, Brockmann M, Schildgen O, Schildgen V. Frontiers in Microbiology 9: 2450.
Pregn-5-en-3β-ol and androst-5-en-3β-ol dicarboxylic acid esters as potential therapeutics for NMDA hypofunction: In vitro safety assessment and plasma stability.
2018. Matousova M, Soucek R, Tloustova E, Slavikova B, Chodounska H, Mertlikova-Kaiserova H, Kudova E. Steroids.
Choanoflagellate transfection illuminates their cell biology and the ancestry of animal septins.
2018. Booth DS, Szmidt-Middleton H, King N. Molecular Biology of the Cell 29(25): 3026-3038.
Glycosyltransferases promote development and prevent promiscuous cell aggregation in the choanoflagellate S. rosetta.
2018. Wetzel LA, Levin TC, Hulett RE, Chan D, King GA, Aldayafleh R, Booth DS, Sigg MA, King N. bioRxiv 384453.
Self-maintaining gut macrophages are essential for intestinal homeostasis.
2018. De Schepper S, Verheijden S, Aguilera-Lizarraga J, Viola MF, Boesmans W, Stakenborg N, Voytyuk I, Schmidt I, Boeckx B, Dierckx de Casterlé I, Baekelandt V, Gonzalez Dominguez E, Mack M, Depoortere I, De Strooper B, Sprangers B, Himmelreich U, Soenen S, Guilliams M, Vanden Berghe P, Jones E, Lambrechts D, Boeckxstaens G. Cell 175(2): 400-415.
Canine mesenchymal stem cells from synovium have a higher chondrogenic potential than those from infrapatellar fat pad, adipose tissue, and bone marrow.
2018. Sasaki A, Mizuno M, Ozeki N, Katano H, Otabe K, Tsuji K, Koga H, Mochizuki M, Sekiya I. PLoS One 13(8): e0202922.
Effect of the bowl structure in an automated cell-isolation device on stromal vascular fraction's isolation yield.
2018. Hahn HM, Jeong KS, Yoo BY, Park JH, Jung HJ, Lee IJ. Journal of Medical Devices 12(4): 044501.
A single-cell transcriptome atlas of the aging Drosophila brain.
2018. Davie K, Janssens J, Koldere D, De Waegeneer M, Pech U, Kreft Ł, Aibar S, Makhzami S, Christiaens V, Bravo González-Blas C, Poovathingal S, Hulselmans G, Spanier KI, Moerman T, Vanspauwen B, Geurs S, Voet T, Lammertyn J, Thienpont B, Liu S, Konstantinides N, Fiers M, Verstreken P, Aerts S. Cell 174(4): 982-998.
Assay for galactose-deficient IgA1 enables mechanistic studies with primary cells from IgA nephropathy patients.
2018. Reily C, Rizk DV, Julian BA, Novak J. Biotechniques 65(2): 71-77.
Hair cell mechanotransduction regulates spontaneous activity and spiral ganglion subtype specification in the auditory system.
2018. Sun S, Babola T, Pregernig G, So KS, Nguyen M, Su SM, Palermo AT, Bergles DE, Burns JC, Müller U. Cell 174(5): 1247-1263.
HIV-1 targets L-selectin for adhesion and induces its shedding for viral release.
2018. Kononchik J, Ireland J, Zou Z, Segura J, Holzapfel G, Chastain A, Wang R, Spencer M, He B, Stutzman N, Kano D, Arthos J, Fischer E, Chun TW, Moir S, Sun P. Nature Communications 9(1): 2825.
Strain-specific contribution of eukaryotic elongation factor 1 gamma to the translation of Influenza A virus proteins.
2018. Sammaibashi S, Yamayoshi S, Kawaoka Y. Frontiers in Microbiology 9: 1446.
Transdermal, but not oral mucosa exposure, causes sublethal leptospirosis in mice.
2018. Nair N, Gomes-Solecki M. bioRxiv 334219.
FGFR signaling regulates resistance of head and neck cancer stem cells to cisplatin.
2018. McDermott SC, Rodriguez-Ramirez C, McDermott SP, Wicha MS, Nör JE. Oncotarget 9(38): 25148-25165.
Canine mesenchymal stem cells treated with TNF-α and IFN-γ enhance anti-inflammatory effects through the COX-2/PGE2 pathway.
2018. Yang HM, Song WJ, Li Q, Kim SY, Kim HJ, Ryu MO, Ahn JO, Youn HY. Research in Veterinary Science 119: 19-26.
Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration.
2018. Nguyen JT, Ray C, Fox AL, Mendonça DB, Kim JK, Krebsbach PH. Science Advances 4(5): eaao5838.
S1P₄ regulates passive systemic anaphylaxis in mice but is dispensable for canonical IgE-mediated responses in mast cells.
2018. Kulinski JM, Proia RL, Larson EM, Metcalfe DD, Olivera A. International Journal of Molecular Sciences 19(5): 1279.
Mechanical tension drives elongational growth of the embryonic gut.
2018. Chevalier NR, de Witte TM, Cornelissen AJM, Dufour S, Proux-Gillardeaux V, Asnacios A. Scientific Reports 8: 5995.
Dual mechanism of type VII collagen transfer by bone marrow mesenchymal stem cell extracellular vesicles to recessive dystrophic epidermolysis bullosa fibroblasts.
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Pan-cancer genome and transcriptome analyses of 1,699 paediatric leukaemias and solid tumours.
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An enclosed rotating floating photobioreactor (RFP) powered by flowing water for mass cultivation of photosynthetic microalgae.
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AEG-1 promotes mesenchymal transition through the activation of Rho GTPases in human glioblastoma cells.
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Brochure
Brochure
Quick Start Guide
Quick Start Guide
Product literature
Product literature
Application note
Application note
Application note
Application note
Application note
Experimental condition
HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
A high density culture of HL-60 cells was counted with the LUNA-FL to verifiy the performance of the instrument. Cells were stained with acridine orange-propidium idodide mixture and then counted. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Human whole blood was collected from a finger tip and diluted in PBS at 1:100. After staining with the AO/PI dyes, white blood cells (WBCs) were counted with the LUNA-FL fluorescence cell counter. Bright field and its corresponding fluorescence image are shown in the left and middle panel, respectively. Area enclosed by the white rectangle is shown in the right panel at high magnification. Note that cells containing nucleic acids (ie., WBCs) were counted and tagged with the green circle. A huge number of red blood cells (RBCs) and platelets seen in the bright field image were not counted by LUNA-FL. No dead WBC was observed in this field of view.
Experimental condition
Jurkat T cells were stained with acridine orange-propidium idodide mixture and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
Mouse neuronal cells were collected directly from brain tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classified by LUNA-FL.
Experimental condition
Mouse PBMC was collected by buffy coat preparation and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Mouse splenocytes were collected directly from spleen tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Mouse thymocytes were collected directly from the thymus tissue and counted with the LUNA-FL fluorescence cell counter. Contaminated red blood cells were nicely discriminated from nucleated cells with LUNA-FL. Green and red circle represents viable and dead nucleated cells automatically classfied by LUNA-FL.
Experimental condition
Yeast (Saccharomyces Cerevisiae) cells were cultured in the YPD medium supplemented with several antibiotics overnight. The yeast cells were artificially killed by heating and mixed with healthy cells (50:50). Mixed cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter.
Experimental condition
A high density 3 micron-sized beads were counted with the LUNA-FL to verifiy the performance of the instrument. Beads were counted at the bright field cell counting mode of LUNA-FL. Orange circles represent the automatically-classified objects by LUNA-FL.
Experimental condition
The number and the viability of yeast cells were measured in a bottle of a Korean rice wine. The wine was diluted at 1:100 with PBS, and the cells were stained with the yeast viability kit (cat# F23202) and then counted with the LUNA-FL fluorescence cell counter
| Cat # | Product | Qty |
|---|---|---|
| L20001 | LUNA-FL™ Automated Fluorescence Cell Counter | 1 unit |
| L12005 | PhotonSlide™, 50 Slides | 1 box |
| L12006 | PhotonSlide™, 500 Slides | 10 boxes |
| L12007 | PhotonSlide™, 1000 Slides | 20 boxes |
| L12001 | LUNA™ Cell Counting Slides, 50 Slides | 1 box |
| L12002 | LUNA™ Cell Counting Slides, 500 Slides | 10 boxes |
| L12003 | LUNA™ Cell Counting Slides, 1000 Slides | 20 boxes |
| L12011 | LUNA™ Reusable Slide | 1 unit |
| L12012 | LUNA™ Reusable Slides | 2 units |
| L12014 | LUNA™ Reusable Slide Coverslips | 10 units |
| T13001 | Trypan Blue Stain, 0.4% | 2 X 1 mL |
| L13002 | Erythrosin B Stain | 2 x 1 mL |
| F23001 | Acridine Orange/Propidium Iodide Stain | 2 x 0.5 mL |
| F23002 | Acridine Orange Stain | 2 x 0.5 mL | <
| F23003 | Propidium Iodide Stain | 2 x 0.5 mL |
| F23202 | Yeast Viability Kit 1 - Propidium Iodide Stain for Yeast (1 x 0.5 mL) - Fluorescein Diacetate Stain (1 x 0.5 mL) - Cell Dilution Buffer (1 x 20 mL) - Fluorescence Signal Enhancer 1 (1 x 0.5 mL) |
1 kit |
| F23212 | Cell Dilution Buffer | 5 x 20 mL |
| F23102 | LUNA™ Fluorescence Calibration Beads | 1 x 0.5 mL |
| B13101 | LUNA™ Standard Beads | 2 x 1 mL |
| F24003 | LUNA-FL™ IQ/OQ Protocol | 1 copy |
| P10001 | LUNA™ Printer | 1 unit |
| P12001 | LUNA™ Printer Paper - thermal, 700 prints | 3 x 2 rolls |
