• BRIGHTFIELD CELL COUNTER

    LUNA-II™ Automated Cell Counter

    Small yet powerful, the LUNA-II™ is cell counting convenience at its finest.
    Simply insert your sample and the LUNA-II™ does the rest: autofocus, adjust light, and count in just 15 seconds.
    download software
The LUNA-II™ is an automated cell counter that gives you the ultimate brightfield cell counting experience. Press count and the LUNA-II™ does the rest – bringing your cells into the sharpest focus, adjusting light levels, capturing a high-resolution image, and analyzing the image to produce accurate cell count and viability data. You can create custom, cell-specific cell counting protocols or simply use the default counting protocol suitable for most cell lines or a PBMC protocol optimized for human peripheral blood mononuclear cells. Live cells are tagged with green circles and dead cells are tagged with red circles, making it easy to verify the accuracy of each count. This little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

WHAT THE LUNA-II™ DOES BEST

  • LUNA-II Automated Cell Counter autofocused counting in 15 seconds
    AUTOFOCUSED COUNTING IN 15 SECONDS

    An autofocusing liquid lens and advanced software produce concentration
    and viability data in as little as 15 seconds.

  • LUNA-II Automated Cell Counter accurate viability data
    Accurate viability data

    The LUNA-II™ can precisely and consistently distinguish live and dead cells from debris.

  • LUNA-II Automated Cell Counter advanced cluster analysis
    Advanced cluster analysis

    Clumpy cells? No problem. An advanced declustering algorithm detects individual cells in clusters.

  • LUNA-II Automated Cell Counter simple recordkeeping
    Simple recordkeeping

    Print data with the built-in printer or export high-resolution images, data,
    and full data reports via USB.

THE SOFTWARE THAT CHANGES EVERYTHING.

CUSTOMIZE & SAVE PROTOCOLS VERIFY RESULTS IMMEDIATELY USE THE DILUTION CALCULATOR GATE CELLS ON HISTOGRAMS

 

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Logos Biosystems LUNA Reusable Slide
Celecoxib treatment of fibrous dysplasia (FD) in a human FD cell line and FD-like lesions in mice with protein kinase A (PKA) defects. Saloustros E, Liu S, Mertz EL, Bhattacharyya N, Starost MF, Salpea P, Nesterova M, Collins M, Leikin S, Stratakis CA. Molecular and cellular endocrinology. 439:165-174.(2017) Scaling and automation of high-throughput single-cell-derived tumor sphere assay chip. Cheng YH, Chen YC, Brien R, Yoon E. Lab on a chip. 16(19):3708-17. Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation. Jeong W, Yang X, Lee J, Ryoo Y, Kim J, Oh Y, Kwon S, Liu D, Son D. Stem cell research & therapy. 7(1):117. p-coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS. Silina YE, Fink-Staube C, Hanselmann RG, Peuschel H, Volmer DA. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1020:6-13. Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems. Bochev I, Belemezova K, Shterev A, Kyurkchiev S. Journal of assisted reproduction and genetics. 33(4):473-80. (2016) Spark plasma sintering of load-bearing iron-carbon nanotube-tricalcium phosphate CerMets for orthopaedic applications. Montufar EB, Horynová M, Casas-Luna M, Diaz-de-la-Torre S, Celko L, Klakurková L, Spotz Z, Diéguez-Trjo G, Fohlerová Z, Drorak K, Zikmund T, Kaiser J. Journal of assisted reproduction and genetics. 33(4):473-80. Application of a non-hazardous vital dye for cell counting with automated cell counters. Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical biochemistry. 492:8-12. (2016)
LUNA-II™ Brochure Application of a non-hazardous vital dye for cell counting with automated cell counters The accuracy and low variability of the LUNA-II™ Logos Biosystems Products Brochure LUNA-II™ software 1.8.9 Software
Compatible Reagents
    Cat # Product Qty MSDS Product Insert
    T13001 Trypan Blue Stain, 0.4%

    2 x 1 mL

    FileAction
    [T13001] Trypan Blue Stain_0.4%.pdfDownload 
    T13001 Trypan Blue Stain, 0.4% VL1801-01.pdfDownload 
    L13002 Erythrosin B Stain

    2 x 1 mL

    FileAction
    [L13002] Erythrosin B Stain.pdfDownload 
    L13002 Erythrosin B Stain VL1801-01.pdfDownload 
    Request CoA   >

    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Clémence Jacquemin, PhD

      INSERM

      Rapid, simple, reliable. This product is easy to use. The fact that one can use a reusable blade is an advantage in terms of cost when one line is to be counted. The results are reliable, we have compared them to those obtained in Molasses cells, against the concentration must be close to 1x10 ^ 6 cells / mL. I am very satisfied with this purchase, which is unanimous within the team.

    • Paul Kim, PhD

      Grambling State University

      This is a nice automated cell counter that is well worth considering. We count cells to set up experiments with a consistent number of input cells and to express the resulting data accurately. An automated cell counter makes this a quick, painless, reproducible process. Tip: get the one with the printer!

    • Tracey Geneau

      Greenfield Ethanol

      I'm testing the LUNA-II and it works great. I work for an ethanol plant and our operators count yeast. This will be a great way to streamline the yeast count results. It will also please our operators.

    + SHOW OUR CUSTOMERS

    WHERE THE LUNA-II™ HAS BEEN CITED

    Citations
    • Celecoxib treatment of fibrous dysplasia (FD) in a human FD cell line and FD-like lesions in mice with protein kinase A (PKA) defects.
      Saloustros E, Liu S, Mertz EL, Bhattacharyya N, Starost MF, Salpea P, Nesterova M, Collins M, Leikin S, Stratakis CA.
      Molecular and cellular endocrinology. 439:165-174.(2017)

    • Scaling and automation of high-throughput single-cell-derived tumor sphere assay chip.
      Cheng YH, Chen YC, Brien R, Yoon E.
      Lab on a chip. 16(19):3708-17.

    • Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation.
      Jeong W, Yang X, Lee J, Ryoo Y, Kim J, Oh Y, Kwon S, Liu D, Son D.
      Stem cell research & therapy. 7(1):117.

    • p-coumaric acid, a novel and effective biomarker for quantifying hypoxic stress by HILIC-ESI-MS.
      Silina YE, Fink-Staube C, Hanselmann RG, Peuschel H, Volmer DA.
      Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1020:6-13.

    • Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems.
      Bochev I, Belemezova K, Shterev A, Kyurkchiev S.
      Journal of assisted reproduction and genetics. 33(4):473-80. (2016)

    • Spark plasma sintering of load-bearing iron-carbon nanotube-tricalcium phosphate CerMets for orthopaedic applications.
      Montufar EB, Horynová M, Casas-Luna M, Diaz-de-la-Torre S, Celko L, Klakurková L, Spotz Z, Diéguez-Trjo G, Fohlerová Z, Drorak K, Zikmund T, Kaiser J.
      Journal of assisted reproduction and genetics. 33(4):473-80.

    • Application of a non-hazardous vital dye for cell counting with automated cell counters.
      Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW. Analytical biochemistry.
      492:8-12. (2016)

    SHOW MORE  +

    Resources

    Downloads
    • Icon
      LUNA-II™

      Brochure

      2018-04-16 | 1.5 MB
    • Icon
      Application of a non-hazardous vital dye for cell counting with automated cell counters

      Product literature

      2018-04-16 | 1.2 MB
    • Icon
      The accuracy and low variability of the LUNA-II™

      Product literature

      2018-04-16 | 906 KB
    • Icon
      Logos Biosystems Products

      Brochure

      2018-04-15 | 2.14 MB
    • Icon
      How to Count Cells An Overview of Cell Counting Methods

      Product literature

      2018-04-13 | 720.79 KB
      • Icon
        LUNA-II™ software 1.9.5

        Software

        2018-04-16 | 8.9 MB
        Videos

        How to Use the LUNA™ Reusable Slide

        Cell DB
          • LUNA-II AutomatedCellCounter A549
          • A549

            Experimental condition

            A549 cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 7 um
            Max. cell size: 60 um
            Declustering level : Medium

          • LUNA-II AutomatedCellCounter AsPC-1
          • AsPC-1

            Experimental condition

            AsPC-1 cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 60 um
            Declustering level: High

          • LUNA-II AutomatedCellCounter C6
          • C6

            Experimental condition

            C6 cells from a routine subculture were counted with the LUNA-II automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 60 um
            Declustering level: Medium

          • CHO

            Experimental condition

            CHO cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 59 %
            Min. cell size: 4 um
            Max. cell size: 60 um
            Declustering level: High

          • LUNA-II AutomatedCellCounter HeLa
          • HeLa

            Experimental condition

            HeLa cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Cell counting result

            Total cell concentration: 9.61 x 10E5 cells/ml
            Live cell concentration: 8.65 x 10E5 cells/ml
            Dead cell concentration: 9.52 x 10E4 cells/ml
            Viabillity: 90.1 %
            Average cell size: 16.9 um

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 60 um
            Declustering level: High

          • LUNA-II AutomatedCellCounter HL-60
          • HL-60

            Experimental condition

            HL-60 cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Result

            Total cell concentration: 1.60 x 10E6 cells/ml
            Live cell concentration: 1.40 x 10E6 cells/ml
            Dead cell concentration: 1.94 x 10E5 cells/ml
            Viabillity: 87.9 %
            Average cell size: 14.8 um

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 60 um
            Declustering level: Medium

          • NIH3T3

            Experimental condition

            NIH3T3 cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Result

            Total cell concentration: 1.45 x 10E6 cells/ml
            Live cell concentration: 1.42 x 10E6 cells/ml
            Dead cell concentration: 2.67 x 10E5 cells/ml
            Viabillity: 98.2 %
            Average cell size: 13.2 um

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 60 um
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter STO
          • STO

            Experimental condition

            STO cells from small amount cell culture were counted with the LUNA-II automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 60 um
            Declustering: Medium

          • LUNA-II AutomatedCellCounter U937
          • U937

            Experimental condition

            U937 cells from routine cell culture were counted with the LUNA-II automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 40 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter Capan-2
          • Capan-2

            Experimental condition

            Capan-2 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 1.24 x 106
            Live cell conc. (cells/ml): 1.23 x 106
            Dead cell conc. (cells/ml): 9.03 x 103
            Viability (%): 99.3
            Average cell size (μm): 11.6

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter DU-145
          • DU 145

            Experimental condition

            DU 145 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 1.31 x 106
            Live cell conc. (cells/ml): 1.23 x 106
            Dead cell conc. (cells/ml): 8.02 x 104
            Viability (%): 93.9
            Average cell size (μm): 14.7

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter H-1299
          • H 1299

            Experimental condition

            H 1299 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 8.28 x 105
            Live cell conc. (cells/ml): 6.32 x 105
            Dead cell conc. (cells/ml): 1.96 x 104
            Viability (%): 76.3
            Average cell size (μm): 19.6

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • H4

            Experimental condition

            H4 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 1.22 x 105
            Live cell conc. (cells/ml): 8.13 x 104
            Dead cell conc. (cells/ml): 4.07 x 104
            Viability (%): 66.7
            Average cell size (μm): 17.7

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter HCT116
          • HCT116

            Experimental condition

            HCT116 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 1.45 x 106
            Live cell conc. (cells/ml): 1.40 x 106
            Dead cell conc. (cells/ml): 4.97 x 104
            Viability (%): 96.6
            Average cell size (μm): 11.6

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter Hep3B
          • Hep3B

            Experimental condition

            Hep3B cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting protocol

            Total cell conc. (cells/ml): 1.12 x 106
            Live cell conc. (cells/ml): 1.10 x 106
            Dead cell conc. (cells/ml): 2.26 x 104
            Viability (%): 98.0
            Average cell size (μm): 14.7

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • Hs 578T

            Experimental condition

            Hs 578T cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 6.10 x 105
            Live cell conc. (cells/ml): 5.28 x 105
            Dead cell conc. (cells/ml): 8.23 x 104
            Viability (%): 86.7
            Average cell size (μm): 18.3

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter MCF7
          • MCF7

            Experimental condition

            MCF7 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 2.89 x 105
            Live cell conc. (cells/ml): 1.99 x 105
            Dead cell conc. (cells/ml): 9.03 x 104
            Viability (%): 68.8
            Average cell size (μm): 16.4

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter PANC-1
          • PANC-1

            Experimental condition

            PANC-1 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting protocol

            Total cell conc. (cells/ml): 1.56 x 106
            Live cell conc. (cells/ml): 1.47 x 106
            Dead cell conc. (cells/ml): 8.58 x 104
            Viability (%): 94.5
            Average cell size (μm): 12.5

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: High

          • PC-3

            Experimental condition

            PC-3 cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 1.51 x 106
            Live cell conc. (cells/ml): 1.48 x 106
            Dead cell conc. (cells/ml): 3.16 x 104
            Viability (%): 97.9
            Average cell size (μm): 16.4

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

          • LUNA-II AutomatedCellCounter U-2-OS
          • U-2 OS

            Experimental condition

            U-2 OS cells from routine cell culture were counted with the LUNA-II automated cell counter

            Cell counting results

            Total cell conc. (cells/ml): 1.08 x 106
            Live cell conc. (cells/ml): 8.24 x 105
            Dead cell conc. (cells/ml): 2.54 x 105
            Viability (%): 76.4
            Average cell size (μm): 13.9

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60%
            Min. cell size (μm): 3
            Max. cell size (μm): 60
            Declustering level: Medium

        show more  +

        ORDERING INFORMATION

        Cat # Product Qty
        L40001 LUNA-II™ Automated Cell Counter (with built-in printer) 1 unit
        L40002 LUNA-II™ Automated Cell Counter (without printer) 1 unit
        L12001 LUNA™ Cell Counting Slides, 50 Slides 1 boxes
        L12002 LUNA™ Cell Counting Slides, 500 Slides 10 boxes
        L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 boxes
        T13101 Trypan Blue Stain, 0.4% 2 x 1 mL
        L13002 Erythrosin B Stain 2 x 1 mL
        B13101 LUNA™ Standard Beads 2 X 1 mL
        P12002 LUNA-II™ Printer Paper - thermal, 700 prints 2 x 5 rolls