Our first, our classic LUNA™ is an automated cell counter for brightfield cell counting is as popular as ever for its small footprint, ergonomic design, and most importantly, reliably accurate cell counts. The LUNA™ captures a high resolution image of your cells and analyzes the image to produce total, live, and dead cell counts in as little as 7 seconds. Get total cell counts for unstained cells and cell viability data for cells stained with trypan blue or erythrosin B. Live cells are tagged with green circles and dead cells are tagged with red circles, making it easy to verify the accuracy of each count. This little counter sits comfortably in a cell culture hood or on your benchtop to fit seamlessly into your workflow.

WHAT THE LUNA™ DOES BEST

  • LUNA Automated Cell Counter 7 seconds to results
    7 Seconds to results

    The LUNA™ acts as your eyes, clicker, and calculator.
    Get concentration and viability data in as little as 7 seconds.

  • LUNA Automated Cell Counter accurate viability data
    Accurate viability data

    The LUNA™ can precisely and consistently distinguish live and dead cells from debris.

  • LUNA Automated Cell Counter advanced cluster analysis
    Advanced cluster analysis

    Clumpy cells? No problem. An advanced declustering algorithm detects individual cells in clusters.

  • LUNA Automated Cell Counter simple recordkeeping
    Simple recordkeeping

    Print data with the built-in printer or export high-resolution images, data, and full data reports via USB.

THE SOFTWARE THAT CHANGES EVERYTHING.

CUSTOMIZE & SAVE PROTOCOLS VERIFY RESULTS IMMEDIATELY USE THE DILUTION CALCULATOR GATE CELLS ON HISTOGRAMS

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA™ Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Logos Biosystems LUNA Reusable Slide
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Lee LT, Ng SY, Chu JY, Sekar R, Harikumar KG, Miller LJ, Chow BK. Federation of American Societies for Experimental Biology journal. 28(6):2632-44 (2014) The nuclear receptor REV-ERBα regulates Fabp7 and modulates adult hippocampal neurogenesis. Schnell A, Chappuis S, Schmutz I, Brai E, Ripperger J, Schaad O, Welzl H, Descombes P, Alberi L, Albrecht U. PLoS One. 9(6):e99883 (2014) Altered expression of 3-betahydroxysterol delta-24-reductase/selective Alzheimer’s disease indicator-1 gene in Huntington’s disease models. Samara A, Galbiati M, Luciani P, Deledda C, Messi E, Peri A, Maggi R. Journal of endocrinological investigation. 37(8):729-737. Inhibitory activity of gold and silica nanospheres to vascular endothelial growth factor (VEGF)-mediated angiogenesis is determined by their sizes Jo DH, Kim JH, Son JG, Piao Y, Lee TG, Kim JH. Nano Research. 7(6):844-852 (2014) Fabrication of dual-pore scaffolds using SLUP (salt leaching using powder) and WNM (wire-network molding) techniques. Cho YS, Hong MW, Kim SY, Lee SJ, Lee JH, Kim YY, Cho YS. Materials Science and Engineering: C. 45:546-55 (2014) Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs. Jeong SH, Kim HK, Song IS, Lee SJ, Ko KS, Rhee BD, Kim N, Mishchenko NP, Fedoryev SA, Stonik VA, Han J. Marine drugs. 12(5):2922-36 (2014) Fabrication and in vitro evaluation of nanosized hydroxyapatite/chitosan-based tissue engineering scaffolds. Sun T, Khan TH, Sultana N. J Nanomaterials. 2014:194680 (2014) Design, synthesis and cytotoxic evaluation of novel 2-(4-N,N-dimethyl) pyridine containing 1,3,4-oxadiazole moiety. Adimule VM. Asian J Biomed Pharmaceut Sci. 4(37):1-5 (2014) Physico-chemical characterization, cytotoxic activity, and biocompatibility studies of tamoxifen-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification method. Lakkadwala S, Nguyen S, Lawrence J, Nauli SM, Nesamony J. Journal of microencapsulation. 31(6):590-9 (2014) Development of a new rapid isolation device for circulating tumor cells (CTCs) using 3D palladium filter and its application for genetic analysis. Yusa A, Toneri M, Masuda T, Ito S, Yamamoto S, Okochi M, Kondo N, Iwata H, Yatabe Y, Ichinosawa Y, Kinuta S, Kondo E, Honda H, Arai F, Nakanishi H. Public Library of Science one. 9(2):e88821 (2014) Fabrication of nanohydroxyapatite/poly(caprolactone) composite microfibers using electrospinning technique for tissue engineering applications. Hassan MI, Sun T, Sultana N. J Nanomaterials 2014:209049 (2014) Real-time infrared thermography detection of magnetic nanoparticle hyperthermia in a murine model under a non-uniform field configuration. Rodrigues HF, Mello FM, Branguinho LC, Zufelato N, Silverira-Lacerda EP, Bakuzis AF. International journal of hyperthermia. 29(8):752-67 (2013) Isolation of living circulating tumor cells (CTCs) from peripheral blood using size-based device and its application to CTC technology in mice. IEEE 2013 International Symposium on Micro-NanoMechatronics and Human Science (MHS). Yusa A, Matsumoto Y, Terazawa K, Masuda T, Arai F, Nakanishi H. MHS2013. DOI: 10.1109/MHS.2013.6710455 (2013) Anti-apoptotic effect of clusterin on cisplatin-induced cell death of retinoblastoma cells. Song HB, Jun HO, Kim JH, Yu YS, Kim KW, Min BH, Kim JH. Oncology Reports. 30(6):2713-8 (2013) Intracellular glutathione pools are heterogeneously concentrated. Montero D, Tachibana C, Winther JR, Appenzeller-Herzog C. Redox Biology. 1(1):508-13 (2013) The interaction between C35 and ΔNp73 promotes chemo-resistance in ovarian cancer cells. Leung TH, Wong SC, Chan KK, Chan DW, Cheung AN, Ngan HY. British Journal of Cancer. 109(4):965-75 (2013) Drug release from porous silicon for stable neural interface. Sun T, Tsang WM, Park WT. Appl Surface Sci. 292:843-851 (2013) Attenuated mitochondrial NADP+-dependent isocitrate dehydrogenase activity induces apoptosis and hypertrophy of H9c2 cardiomyocytes. Lee JH, Park JW. Biochimie. 99:110-8. (2013) Targeting mutant p53 by a SIRT1 activator YK-3-237 inhibits the proliferation of triple-negative breast cancer cells. Yi YW, Kang HJ, Kim HJ, Kong Y, Brown ML, Bae I. Oncotarget. 4(7):984-94 (2013) Inhibition of checkpoint kinase 2 (CHK2) enhances sensitivity of pancreatic adenocarcinoma cells to gemcitabine. Duong HQ, Hong YB, Kim JS, Lee HS, Yi YW, Kim YJ, Wang A, Zhao W, Cho CH, Seong YS, Bae I. Journal of cellular and molecular medicine. 17(10):1261-70 (2013) Characterization of enterovirus 71 replication kinetics in human colorectal cell line, HT29. Liu YLE, Timms P, Hafner LM, Tan TL, Tan KH, Tan EL. SpringerPlus. 2(1):267 (2013) Inhibition of the PI3K/AKT pathway potentiates cytotoxicity of inhibitors for EGFR kinase in triple-negative breast cancer cells. Yi YW, Hong W, Kang HJ, Kim HJ, Zhao W, Wang A, Seong YS, Bae I. Journal of cellular and molecular medicine. 17(5):648-56 (2013) Inhibition of NRF2 by PIK-75 augments sensitivity of pancreatic cancer cells to gemcitabine. Duong HQ, Yi YW, Kang HJ, Hong YB, Tang W, Wang A, Seong YS, Bae I. International journal of oncology. 44(3):959-69 (2013) The effects of non-thermal atmospheric pressure plasma jet on attachment of osteoblast. Kwon JS, Kim YH, Choi EH, Kim KN. Current Applied Physics 13:S42-S47. (2013) Towards an optimal and unbiased approach for tumor cell isolation. Sajay BNG, Chan CP, Ahmad H, Chung WC, Puiu PD, Rahman ARA. Biomedical Microdevices. 15(4):699-709 (2013) BML-275, an AMPK inhibitor, induces DNA damage, G2/M arrest and apoptosis in human pancreatic cancer cells. Duong HQ, Hwang JS, Kim HJ, Seong YS, Bae I. International Journal of Oncology. 41(6):2227-36 (2012) Aldehyde dehydrogenase 1A1 confers intrinsic and acquired resistance to gemcitabine in human pancreatic adenocarcinoma MIA PaCa-2 cells. Duoung HQ, Hwang JS, Kim HJ, Kang HJ, Seong YS, Bae I. International journal of oncology. 41(3):855-61 (2012) FAS1 domain protein inhibits VEGF165-induced angiogenesis by targeting the interaction between VEGFR-2 and αvβ3 integrin. Nam JO, Son HN, Jun E, Cha K, Lee BH, Park RW, Kim IS. Molecular Cancer Research. 10(8):1010-20 (2012) BRCA1 negatively regulates IGF-1 expression through an estrogen-responsive element-like site. Kang HJ, Yi YW, Kim HJ, Hong YB, Seong YS, Bae I. Cell Death & Disease. 3:e336.
LUNA™ Quick Start Guide LUNA™ Brochure Logos Biosystems Products Brochure Application of a non-hazardous vital dye for cell counting with automated cell counters Product literature Autoexposure mode update Notice LUNA™ software ver. 3.4.0 Software
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    T13001 Trypan Blue Stain, 0.4%

    2 x 1 mL

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    T13001 Trypan Blue Stain, 0.4% VL1801-01.pdfDownload 
    L13002 Erythrosin B Stain

    2 x 1 mL

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    L13002 Erythrosin B Stain VL1801-01.pdfDownload 
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    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Megha Haridas Upadya

      Nanyang Technological University, Singapore

      We use the LUNA Automated Cell Counter in cell culture to count a variety of mammalian cell lines. We find that the cell counter is user friendly, quick and efficient. Since purchasing the cell counter, the time taken to complete experiments has reduced substantially.

    • Edmund Lui, PhD

      Singapore Polytechnic

      I have used the LUNA for my Virology studies. It has a small and lab friendly footprint for the bench-top. I also like the automatic calculation function which was easy to use and provided me with very accurate and reproducible results for my cell studies. LUNA has provided me the fastest platform to count my cells as compared to manual counting. So far after evaluating several brands, LUNA still proves to be the fastest. I am very impressed and glad with the purchase.

    • Stephanie Eng

      Memorial Sloan-Kettering Cancer Center

      The LUNA has been a great addition to our lab and is so small that we can just place it away under the drawer whenever it is not in use. I have found the cell counter to be more accurate than the V*** for small seeding condition plating.

    • Kevin

      Case Western Reserve University

      We tested the LUNA against three other manufacturers - the B, M, L, company`s products. The LUNA was just as good as, if not better than, all the competition. In addition, the pricing of the device and the corresponding consumables was a winner. We're very happy with that purchase.

    • Monique Heitmeier, PhD

      Washington University in St. Louis

      We LOVE the LUNA cell counter! Makes my life easier for sure!

    + SHOW OUR CUSTOMERS

    WHERE THE LUNA™ HAS BEEN CITED

    Citations
    • The fabrication of well-interconnected polycaprolactone/hydroxyapatite composite scaffolds, enhancing the exposure of hydroxyapatite using the wire-network molding technique.
      Cho YS, Hong MW, Jeong HJ, Lee SJ, Kim YY, Cho YS.
      J Biomed Mater Res B Appl Biomater. 105(8):2315-2325. (2016)

    • Disruption of STAT3-DNMT1 interaction by SH-I-14 induces re-expression of tumor suppressor genes and inhibits growth of triple-negative breast tumor.
      Kang HJ, Yi YW, Hou SJ, Kim HJ, Kong Y, Bae I, Brown ML.
      Oncotarget. 8(48):83457-83468 (2015)

    • Celecoxib treatment of fibrous dysplasia (FD) in a human FD cell line and FD-like lesions in mice with protein kinase A (PKA) defects.
      Saloustros E, Liu S, Mertz EL, Bhattacharyya N, Starost MF, Salpea P, Nesterova M, Collins M, Leikin S, Stratakis CA.
      Molecular and cellular endocrinology. 439:165-174. (2017)

    • Construction and characterization of osteogenic and vascular endothelial cell sheets from rat adipose-derived mesenchymal stem cells.
      Zhang H, Yu N, Zhou Y, Ma H, Wang J, Ma X, Liu J, Huang J, An Y.
      Tissue & cell. 48(5):488-95. (2016)

    • N-acetyl-L-cysteine exacerbates generation of IL-10 in cells stimulated with endotoxin in vitro and produces antipyresis via IL-10 dependent pathway in vivo.
      Wrotek S, Jędrzejewski T, Piotrowski J, Kozak W.
      Immunology letters. 177:1-5. (2016)

    • Dual pharmacological inhibition of glutathione and thioredoxin systems synergizes to kill colorectal carcinoma stem cells.
      Tanaka G, Inoue KI, Shimizu T, Akimoto K, Kubota K.
      Cancer medicine. 5(9):2544-57. (2016)

    • Encapsulation of curcumin in polyelectrolyte nanocapsules and their neuroprotective activity.
      Szczepanowicz K, Jantas D, Piotrowski M, Staroń J, Leśkiewicz M, Regulska M, Lasoń W, Warszyński P.
      Nanotechnology. 27(35):355101.

    • Copper (II) oxide nanoparticles induced nephrotoxicity in vitro conditions.
      Mahmoud A, Elif GE, Gül Ö.
      ApplIn Vitro Toxicol. doi:10.1089/aivt.2016.0008. (2016)

    • Binucleated HeLa cells are formed by cytokinesis failure in starvation and keep the potential of proliferation.
      Nishimura K, Watanabe S, Hayashida R, Sugishima S, Iwasaka T, Kaku T.
      Cytotechnology. 68(4):1123-30. (2016)

    • Vitamin E δ-tocotrienol triggers endoplasmic reticulum stress-mediated apoptosis in human melanoma cells.
      Marelli MM, Marzagalli M, Moretti RM, Beretta G, Casati L, Comitato R, Gravina GL, Festuccia C, Limonta P.
      Scientific reports. 6:30502. (2016)

    • Gold nanocrystals with well-defined crystallographic {111} facets suppress pathological neovascularization.
      Jo DH, Hong JW, Kim JH, Han SW, Kim JH.
      Journal of biomedical nanotechnology. 12(7):1520-26. (2016)

    • In vitro toxicological assessment of magnesium oxide nanoparticle exposure in several mammalian cell types.
      Mahmoud A, Ezgi Ö, Merve A, Özhan G.
      International journal of toxicology. 35(4):429-37. (2016)

    • Structure-Based Drug Discovery for Prion Disease Using a Novel Binding Simulation.
      Ishibashi D, Nakagaki T, Ishikawa T, Atarashi R, Watanabe K, Cruz FA, Hamada T, Nishida N.
      EBioMedicine. 9:238-249. (2016)

    • Progesterone-dependent induction of phospholipase C-related catalytically inactive protein 1 (PRIP-1) in decidualizing human endometrial stromal cells.
      Muter J, Brighton PJ, Lucas ES, Lacey L,Shmygol A, Quenby S, Blanks AM, Brosen JJ. Endocrinology.
      157(7):2883-93.

    • Polysaccharide gel nanoparticles modified by the Layer-by-Layer technique for biomedical applications.
      Podgórna K, Jankowska K, Szczepanowicz K.
      Colloids Surf APhysicochemEng Asp. doi:10.1016/j.colsurfa.2016.07.067. (2016)

    • Gene expression in mouse thyrotrope adenoma: transcription elongation factor stimulates proliferation.
      Gergics P, Christian HC, Choo MS, Ajmal A, Camper SA.
      Endocrinology. 157(9):3631-46. (2016)

    • Recombinant AAV-crumbs homologue composition and method for treating LCA-8 and progressive RP.
      Wijnholds J, Pellissier LPF.
      US Patent Application 2016019474.

    • Coptis Japonica makino extract suppresses angiogenesis through regulation of cell cycle-related proteins.
      Kim SH, Kim EC, Kim WJ, Lee MH, Kim SY, Kim TJ.
      Bioscience, biotechnology, and biochemistry. 80(6):1095-106.

    • Polysaccharide peptides from Coriolus versicolor exert differential immunomodulatory effects on blood lymphocytes and breast cancer cell line MCF-7 in vitro.
      Kowalczewska M, Piotrowski J,Jędrzejewski T, Kozak W.
      Immunology letters. 174:37-44.

    • Polyelectrolyte multilayer assembled from IL-10 plasmid DNA and TGF-β siRNA facilitates chronic pancreatitis treatment.
      Feng WM, Guo HH, Xue T, Wang X, Tang CW, Bao Y, Gong H, Cui G.
      RSC Adv. 6:60171-60179. (2016)

    • Capture of tumor cells on anti-EpCAM-functionalized poly(acrylic acid)-coated surfaces.
      Andree KC, Barradas AMC, Nguyen AT, Mentink A, Stojanovic I, Baggerman J, van Dalum J, van Rijn CJM, Terstappen LWMM.
      ACS applied materials & interfaces. 8(23):14349-56.

    • Synthesis, characterization, and in vitro antitumor activity of ruthenium(II) polypyridyl complexes tethering EGFR-inhibiting 4-anilinoquinazolines.
      Du J, Kang Y, Zhao Y, Zheng W, Zhang Y, Lin Y, Wang Z, Wang Y, Luo Q, Wu K, Wang F.
      Inorg Chem 55(9):4595-605.

    • Serotonin- and dopamine-related gene expression in db/db mice islets and in MIN6 β-cells treated with palmitate and oleate.
      Cataldo LR, Mizgier ML, Busso D, Olmos P, Galgani JE, Valenzuela R, Mezzano D, Aranda E, Cortés VA, Santos JL.
      Journal of diabetes research. 2016:3793781. (2016)

    • Visualization of HDAC9 spatiotemporal subcellular localization in primary neuron cultures.
      Sugo N, Yamamoto N.
      Methods in molecular biology. 1436:119-27.

    • PEDOT: PSS-containing nano hydroxyapatite/chitosan conductive bionanocomposite scaffold: fabrication and evaluation.
      Sultana N, Lazi A, Sun T.
      J Nanomaterials. (2016)

    • Culture medium composition for improving regenerative capacity of stem cells, and stem cell culturing method using same.
      Ra JC, Kang SK, Jo JY.
      US Patent Application 20160090573.

    • Repurposing treprostinil for enhancing hematopoietic progenitor cell transplantation.
      Kazemi Z, Bergmayr C, Prchal-Murphy M, Javaheri T, Themann M, Pham HTT, Strohmaier W, Sexl V, Freissmuth M, Zebedin-Brandl E.
      Molecular pharmacology. 89(6):630-44.

    • Selective killing effects of cold atmospheric pressure plasma with NO induced dysfunction of epidermal growth factor receptor in oral squamous cell carcinoma.
      Lee JH, Om IY, Kim YH, Kim KM, Choi EH, Kim KN.
      PloS one. 11(2):e0150279. (2016)

    • Cardiac adipose-derived stem cells exhibit high differentiation potential to cardiovascular cells in C57BL/6 mice.
      Nagata H, Il M, Kohbayashi E, Hoshiga M, Hanafusa T, Asahi M.
      Stem cells translational medicine. 5(2):141-51. (2016)

    • In vitro toxicological assessment of cobalt ferrite nanoparticles in several mammalian cell types.
      Abudayyak M, Gurkaynak TA, Özhan G.
      Biological trace element research. 175(2):458-465. (2016)

    • Hyaluronan stimulates pancreatic cancer cell motility.
      Cheng XB, Kohi S, Koga A, Hirata K, Sato N.
      Oncotarget 7(4):4829-40. (2016)

    • C-terminal region of GADD34 regulates eIF2α dephosphorylation and cell proliferation in CHO-K1 cells.
      Otsuka R, Harada N, Aoki S, Shirai K, Nishitsuji K, Nozaki A, Hatakeyama A, Shono M, Mizusawa N, Yoshimoto K, Nakaya Y, Kitahata H, Sakaue H.
      Cell stress & chaperones. 21(1):29-40.

    • Celecoxib reduces glucocorticoids in vitro and in a mouse model with adrenocortical hyperplasia.
      Liu S, Saloustros E, Berthon A, Starost M, Sahut-Barnola I, Salpea P, Szarek E, Faucz FR, Martinez A, Stratakis CA.
      Endocrine-related cancer. 23(1):15-25. (2016)

    • MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
      Sakuma T, Nakade S, Sakane Y, Suzuki KI, Yamamoto T.
      Nature protocols. 11(1):118-33

    • Anti-lymphocytic enzyme L-asparaginase from Streptomyces antitioticus of Western Ghat Soil.
      Salimath MB, Shobha KS, Onkarappa R.
      Int ConfAdvBiotechnol (BioTech) Proc 40-46. (2016)

    • LL-37-induced host cell cytotoxicity depends on cellular expression of the globular C1q receptor (p33).
      Svensson D, Wilk L, Mörgelin M, Herwald H, Nilsson BO.
      The Biochemical journal. 473:87-98. (2016)

    • Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery.
      Gho CG, Schomann T, de Groot SC, Frijns JHM, Rivolta MN, Neumann MHA, Huisman MA.
      Cytotechnology. 68(5):1849-58.(2015)

    • Cytotoxicity of glass ionomer cements containing silver nanoparticles.
      Siqueira PC, Magalhães APR, Pires WC, Pereira FC, Silveira-Lacerda EP, Carrião MS, Bakuzis AF, Souza-Costa CA, Lopes LG, Estrela C.
      Journal of clinical and experimental dentistry. 7(5):e622-7. (2015)

    • Functional characterization of Nupr1L, a novel p53-regulated isoform of the high mobility group (HMG)-related protumoral protein Nupr1.
      Lopez MB, Garcia MN, Grasso D, Bintz J, Molejon MI, Velez G, Lomberk G, Neira JL, Urrutia R, Inovanna J.
      Journal of cellular physiology. 230(12):2936-50.(2015)

    • Functional characterization of Nupr1L, a novel p53-regulated isoform of the high mobility group (HMG)-related protumoral protein Nupr1.
      Lopez MB, Garcia MN, Grasso D, Bintz J, Molejon MI, Velez G, Lomberk G, Neira JL, Urrutia R, Inovanna J.
      Journal of cellular physiology. 230(12):2936-50. (2015)

    • An Involvement of PI3-K/Akt Activation and Inhibition of AIF Translocation in Neuroprotective Effects of Undecylenic Acid (UDA) Against Pro-Apoptotic Factors-Induced Cell Death in Human Neuroblastoma SH-SY5Y Cells.
      Jantas D, Piotrowski M, Lason W.
      Journal of cellular biochemistry. 116(12):2882-95. (2015)

    • A CXCR4 antagonist leads to tumor suppression by activation of immune cells in a leukemia-induced microenvironment.
      Han AR, Lee JY, Kim HJ, Min WS, Park G, Kim SH.
      Oncology reports. 34(6):2880-8. (2015)

    • Cytotoxic effect of purified L-asparaginase from Salinicoccus sp. M KJ997975.
      Bhat M, Marar T.
      Int J CurrMicrobiol App Sci. 4:701-712. (2015)

    • Polyelectrolyte-coated nanocapsules containing undecylenic acid: synthesis, biocompatibility and neuroprotective properties.
      Piotrowski M, Jantas D, Szczepanowicz K, Łukasiewicz S, Lasoń W, Warszyńskia P.
      Colloids Surf B Biointerfaces 135:8-17. (2015)

    • Global histone modifications in fumonisin B1 exposure in rat kidney epithelial cells.
      Sancak D, Ozden S.
      Toxicol In Vitro 29(7):1809-15. (2015)

    • Electrochemical preparation and characterization of magnetic core-shell nanowires for biomedical applications.
      Gispert C, Serrà A, Alea ME, Rodrigues M, Gómez E, Mora M, Sagristá MLI, Pérez-García L, Vallés E.
      Electrochem Commun 2016;63:18-21.

    • Chemically defined culture and cardiomyocyte differentiation of human pluripotent stem cells.
      Burridge PW, Holmström A, Wu JC.
      Current Protocols in Human Genetics. 87(1): 21.3.1-15 (2015)

    • Tumor suppressor Nf2/merlin drives Schwann cell changes following electromagnetic field exposure through Hippo-dependent mechanisms.
      Colciago A, Melfi S, Giannotti G, Bonalume V, Ballabio M, Caffino L, Fumagalli F, Magnaghi V.
      Cell death discovery. 1:15021. (2015)

    • Engineered microstructure granules for tailored drug release rate.
      Hong MH, Choi HJ, Ko YM, Lee YK.
      Biotechnology and bioengineering. 112(9):1936-47. (2015)

    • Engineered microstructure granules for tailored drug release rate.
      Hong MH, Choi HJ, Ko YM, Lee YK.
      Biotechnology and bioengineering. 112(9):1936-47. (2015)

    • Iron for proliferation of cell lines and hematopoietic progenitors: nailing down the intracellular functional iron concentration.
      Pourcelot E, Lénon M, Mobilia N, Cahn JY, Arnaud J, Fanchon E, Moulis JM, Mossuz P.
      Biochimica et biophysica acta. 1853(7):1596-605.(2015)

    • Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6.
      Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I.
      International journal of oncology. 47(1):122-32.

    • Iron for proliferation of cell lines and hematopoietic progenitors: nailing down the intracellular functional iron concentration.
      Pourcelot E, Lénon M, Mobilia N, Cahn JY, Arnaud J, Fanchon E, Moulis JM, Mossuz P.
      Biochimica et biophysica acta. 1853(7):1596-605. (2015)

    • Dual inhibition of EGFR and MET induces synthetic lethality in triple-negative breast cancer cells through downregulation of ribosomal protein S6.
      Yi YW, You K, Bae EJ, Kwak SJ, Seong YS, Bae I.
      International journal of oncology. 47(1):122-32. (2015)

    • Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib.
      Yi YW, Park JS, Kwak SJ, Seong YS.
      Anticancer Res 35(7):3829-38. (2015)

    • Design, synthesis and anticancer activity of functionalized spiro-quinolines with barbituric and thiobarbituric acids.
      Bhaskarachar RK, Revanasiddappa VG, Hegde S, Balakrishna JP, Reddy SY.
      Med Chem Red 2015;24:3516-3528.

    • Application of a non-hazardous vital dye for cell counting with automated cell counters.
      Kim SI, Kim HJ, Lee HJ, Lee K, Hong D, Lim H, Cho K, Jung N, Yi YW.
      Anal Biochem. 2016;492:8-12.

    • Mutations in the gene encoding the E2 conjugating enzyme UBE2T cause Faconi anemia.
      Hira A, Yoshida K, Sato K, Okuno Y, Shiraishi Y, Chiba K, Tanaka H, Miyano S, Shimamoto A, Tahara H, Ito E, Kojima S, Kurumizaka H, Ogawa S, Takata M, Yabe H, Yabe M.
      AM J HUM GENET. 96(6):1001-7.

    • Autocrine/paracrine function of globular adiponectin: inhibition of lipid metabolism and inflammatory response in 3T3-L1 adipocytes.
      Lazra Y, Falach A, Frenkel L, Rozenberg K, Sampson SR, Rosenzweig T.
      Journal of cellular biochemistry. (2015)

    • Anti-inflammation and anti-fibrosis with PEGylated, apigenin loaded PLGA nanoparticles in chronic pancreatitis disease.
      Feng WM, Gu HH, Xue T, Wang X, Tang CW, Ying B, Gong H, Cui G.
      RSC Adv. 5:83628-83635. (2015)

    • Surface characteristics, biodegradability and biocompatibility of porous silicon for microfabricated neural electrode.
      Sun T, Tsan WM, Park WT, Merugu S.
      Journal of nanoscience and nanotechnology. 15(4):2821-8. (2015)

    • Comparative analysis of NRF2-responsive gene expression in AsPC-1 pancreatic cancer cell line. Yi YW, Oh S. Genes & genomics. 37:97-109.(2015)

    • Water extract of Cinnamomum cassia suppresses angiogenesis through inhibition of VEGF receptor 2 phosphorylation.
      Kim EC, Kim HJ, Kim TJ.
      BiosciBiotechnol Biochem 79(4):617-24. (2015)

    • Cytotoxic effect of purified L-asparaginase from Salinicoccus sp. M KJ997975.
      Bhat M, Marar T.
      Int J CurrMicrobiol App Sci. 4:701-712. (2015)

    • Validation of three viable-cell counting methods: manual, semi-automated, and automated.
      Cadena-Herrera D, Lala JEED, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E.
      Biotechnology reports. 7:9-16. (2015)

    • Integrated 3D conducting polymer-based bioelectronics for capture and release of circulating tumor cells.
      Hsiao YS, Ho BC, Yan HX, Kuo C, Chueh DY, Yu HH, Chen P.
      J Mater Chem B Mater Biol Med. 3:5103-5110. (2015)

    • Zinc oxide nanoparticles induced cyto- and genotoxicity in kidney epithelial cells.
      Uzar NK, Abudayyak M, Akcay N, Algun G, Özhan G.
      Toxicology mechanisms and methods. 25(4):334-9. (2015)

    • Water extract of Cinnamomum cassia suppresses angiogenesis through inhibition of VEGF receptor 2 phosphorylation.
      Kim EC, Kim HJ, Kim TJ.
      Bioscience, biotechnology, and biochemistry. 79(4):617-24. (2015)

    • Inhibition of adhesion, proliferation and invasion of primary endometriosis, endometrial stromal and ovarian carcinoma cells by a non-hyaluronan adhesion barrier gel.
      Renner SP, Strissel PL, Beckmann MW, Lermann J, Burghaus S, Hackl J, Fasching PA, Strick R.
      BioMed Res Int. 2015:450468. (2015)

    • Integrated 3D conducting polymer-based bioelectronics for capture and release of circulating tumor cells.
      Hsiao YS, Ho BC, Yan HX, Kuo C, Chueh DY, Yu HH, Chen P.
      J Mater Chem B Mater Biol Med. 3:5103-5110. (2015)

    • The anti-angiogenic potential of a Phellodendronamurense hot water extract in vitro and ex vivo.
      Kim EC, Kim SH, Bae K, Kim HS, Gelinsky M, Kim TJ.
      J Life Sci 25:693-702. (2015)

    • Validation of three viable-cell counting methods: manual, semi-automated, and automated.
      Cadena-Herrera D, Lala JEED, Ramírez-Ibañez ND, López-Morales CA, Pérez NO, Flores-Ortiz LF, Medina-Rivero E.
      Biotechnology reports. 7:9-16.(2015)

    • Relief of delayed oxidative stress by ascorbic acid can suppress radiation-induced cellular senescence in mammalian fibroblast cells.
      Kobashigawa S, Kashino G, Mori H, Watanabe M.
      Mechanisms of ageing and development. 146-148:65-71. (2015)

    • Relief of delayed oxidative stress by ascorbic acid can suppress radiation-induced cellular senescence in mammalian fibroblast cells.
      Kobashigawa S, Kashino G, Mori H, Watanabe M.
      Mechanisms of ageing and development. 146-148:65-71.(2015)

    • β-TrCP1 degradation is a novel action mechanism of PI3K/mTORC2 inhibitors in triple-negative breast cancer cells.
      Yi YW, Kang HJ, Bae EJ, Oh S, Soeng YS, Bae I.
      Experimental & molecular medicine. 47:e143.

    • Nucleoredoxin promotes adipogenic differentiation through regulation of Wnt/β-catenin signaling.
      Bahn YJ, Lee KP, Lee SM, Choi JY, Seo YS, Kwon KS.
      Journal of lipid research. 56(2):294-303. (2015)

    • Nucleoredoxin promotes adipogenic differentiation through regulation of Wnt/β-catenin signaling.
      Bahn YJ, Lee KP, Lee SM, Choi JY, Seo YS, Kwon KS.
      Journal of lipid research. 56(2):294-303.

    • Tissue-specific functions and regulation of protein kinase CK2.
      Welker S, Servas C, Rui M, Götz C, Montenarth M.
      Adv Biochem Health Dis. 12:109-123. (2015)

    • Tissue-specific functions and regulation of protein kinase CK2.
      Welker S, Servas C, Rui M, Götz C, Montenarth M.
      Advances in Biochemistry in Health and Disease 12:109-123. (2015)

    • Dexamethasone enhances osteogenic differentiation of bone marrow- and muscle-derived stromal cells and augments ectopic bone formation induced by bome morphogenetic protein-2.
      Yuasa M, Yamada T, Taniyama T, Masaoka T, Xuetao W, Yoshii T, Horie M, Yasuda H, Uemura T, Okawa A, Sotome S.
      PloS one. 10(2):e0116462. (2015)

    • Inhibition of adhesion, proliferation and invasion of primary endometriosis, endometrial stromal and ovarian carcinoma cells by a non-hyaluronan adhesion barrier gel.
      Renner SP, Strissel PL, Beckmann MW, Lermann J, Burghaus S, Hackl J, Fasching PA, Strick R.
      BioMed Res Int. 2015:450468. (2015)

    • Intrinsic TGF-β2-triggered SDF-1-CXCR4 signaling axis is crucial for drug resistance and a slow-cycling state in bone marrow-disseminated tumor cells.
      Nakamura T, Shinriki S, Jono H, Guo J, Ueda M, Hayashi M, Yamashita S, Zijlstra A, Nakayama H, Hiraki A, Shinohara M, Ando Y.
      Oncotarget. 6(2):1008-19. (2015)

    • Alteration in global DNA methylation after bisphenol a exposure in MCF-7 cells.
      Şenyildiz M, Őzden S. J Fac
      Pharm Istanbul. 45:153-164.(2015)

    • Osteopontin-integrin αvβ3 axis is crucial for 5-fluorouracil resistance in oral squamous cell carcinoma.
      Nakamura T, Shinriki S, Jono H, Ueda M, Nagata M, Guo J, Hayashi M, Yoshida R, Ota T, Ota K, Kawahara K, Nakagawa Y, Yamshita S, Nakayama H, Hiraki A, Shinohara M, Ando Y.
      FEBS letters. 589(2):231-9. (2015)

    • Novel anti-angiogenic activity in Rubuscoreanus Miquel water extract suppresses VEGF-induced angiogenesis.
      Kim EC, Kim HJ, Kim TJ.
      Biomedical Sci Lett 20:209-220. (2014)

    • Hot-water extract of RubusCoreanus Miquel suppresses VEGF-induce angiogenesis.
      Kim EC, Kim HJ, Kim TJ. J Life Sci
      Journal of Life Science. 24(12):1345-1355 (2014)

    • HER2 confers drug resistance of human cancer cells through activation of NRF2 by direct interaction.
      Kang HJ, Yi YW, Hong YB, Kim HJ, Jang YJ, Seong YS, Bae I.
      Scientific reports. 4:7201

    • A comparative study of three cytotoxicity test methods for nanomaterials using sodium lauryl sulfate.
      Kwon JS, Kim KM, Kim KN. J
      Journal of nanoscience and nanotechnology. 14(10):8043-7 (2014)

    • EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling.
      Arasada RR, Amann JM, Rahman MA, Huppert SS, Carbone DP.
      Cancer research. 74(19):5572-84 (2014)

    • Seeding cells on calcium phosphate scaffolds using hydrogel enhanced osteoblst proliferation and differentiation.
      Hong MH, Kim SM, Om JY, Kwon N. Lee YK.
      Annals of Biomedical Engineering. 42(7):1424-35. (2014)

    • Synthesis, characterization and cytotoxic evaluation of novel derivatives of 1,3,4-oxadiazole containing 5-phenyl thiophene moiety.
      Adimule V, Medapa S, Jagadeesha AH, Kumar LS, Rao PK.
      IIOSR Journal of Pharmacy and Biological Sciences. 9:42-48 (2014)

    • Cell isolation system for rare circulating tumor cell. 2014 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2014).
      Masuda T, Yilling S, Eui SW, Niimi M, Yusa A, Nakanishi H, Ara F.
      DOI: 10.1109/IROS.2014.6943230. (2014)

    • Molecular Insight Pharmaceuticals, Inc. Organ protection in PSMA-targeted radionuclide therapy of prostate.
      Babich JW, Shawn H, Joyal J.
      US Patent Application 20160067361.

    • Role of Bcl-xL/Beclin-1 in synergistic apoptotic effects of secretory TRAIL-armed adenovirus in combination with mitomycin C and hyperthermia on colon cancer cells.
      Kim SY, Lee DH, Song X, Bartlett DL, Kwon YT, Lee YJ.
      Apoptosis. 19(11):1603-15 (2014)

    • Novel carbazole inhibits phospho-STAT3 through induction of protein tyrosine phosphatase PTPN6.
      Hou SJ, Yi YW, Kang HJ, Zhang L, Kim HJ, Kong Y, Liu Y, Wang K, Kong HS, Grindrod S, Bae I, Brown ML.
      Journal of medicinal chemistry. 57(15):6342-53 (2014)

    • Cell immobilization on polymer by air atmospheric pressure plasma jet treatment.
      Lee JW, Kwon JS, Om JY, Kim YH, Choi EH, Kim KM, Kim KN.
      Japan Society of Applied Physics. 53:086202 (2014)

    • Enterovirus71 (EV71) utilize host microRNAs to mediate host immune system enhancing survival during infection.
      Lui YLE, Tan TL, Woo WH, Timms P, Hafner LM, Tan KH, Tan EL.
      PloS one. 9(7):e102997 (2014)

    • Characterization and evaluation of 5-fluorouracil-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification technique.
      Patel MN, Lakkadwala S, Majrad MS, Injeti ER, Gollmer SM, Shah ZA, Boddu SHS, Nesamony J.
      AAPS PharmSciTech. 15(6):1498-508 (2014)

    • Adipose tissue-derived mesenchymal stem cells in long-term dialysis patients display downregulation of PCAF expression and poor angiogenesis activation.
      Yamanaka S, Yokote S, Yamada A, Katsuoka Y, Izuhara L, Shimada Y, Omura N, Okano HJ, Ohki T, Yokko T.
      PLoS One 9(7):e102311.

    • Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells.
      Bieler J, Cannavo R, Gustafson K, Gobet C, Gatfield D, Naef F.
      Molecular Systems Biology. 10:739 (2014)

    • Mesenchymal stromal cells promote tumor progression in fibrosarcoma and gastric cancer cells.
      Song B, Kim B, Choi SH, Song KY, Chung YG. Lee YS, Park G.
      Korean Journal of Pathology. 48(3):217-24 (2014)

    • Design of polycaprolactone vascular grafts.
      Yalcin I, Horakova J, Mikes P, Sadikoglu TG, Domin R, Lukas D.
      J Ind Text 45(5):813-833 (2014)

    • Transmembrane peptides as unique tools to demonstrate the in vivo action of a cross-class GPCR heterocomplex.
      Lee LT, Ng SY, Chu JY, Sekar R, Harikumar KG, Miller LJ, Chow BK.
      Federation of American Societies for Experimental Biology journal. 28(6):2632-44 (2014)

    • The nuclear receptor REV-ERBα regulates Fabp7 and modulates adult hippocampal neurogenesis.
      Schnell A, Chappuis S, Schmutz I, Brai E, Ripperger J, Schaad O, Welzl H, Descombes P, Alberi L, Albrecht U.
      PLoS One. 9(6):e99883 (2014)

    • Altered expression of 3-betahydroxysterol delta-24-reductase/selective Alzheimer’s disease indicator-1 gene in Huntington’s disease models.
      Samara A, Galbiati M, Luciani P, Deledda C, Messi E, Peri A, Maggi R.
      Journal of endocrinological investigation. 37(8):729-737 (2014)

    • Inhibitory activity of gold and silica nanospheres to vascular endothelial growth factor (VEGF)-mediated angiogenesis is determined by their sizes
      Jo DH, Kim JH, Son JG, Piao Y, Lee TG, Kim JH.
      Nano Research. 7(6):844-852 (2014)

    • Fabrication of dual-pore scaffolds using SLUP (salt leaching using powder) and WNM (wire-network molding) techniques.
      Cho YS, Hong MW, Kim SY, Lee SJ, Lee JH, Kim YY, Cho YS.
      Materials Science and Engineering: C. 45:546-55 (2014)

    • Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs.
      Jeong SH, Kim HK, Song IS, Lee SJ, Ko KS, Rhee BD, Kim N, Mishchenko NP, Fedoryev SA, Stonik VA, Han J.
      Marine drugs. 12(5):2922-36 (2014)

    • Fabrication and in vitro evaluation of nanosized hydroxyapatite/chitosan-based tissue engineering scaffolds.
      Sun T, Khan TH, Sultana N. J Nanomaterials.
      2014:194680 (2014)

    • Design, synthesis and cytotoxic evaluation of novel 2-(4-N,N-dimethyl) pyridine containing 1,3,4-oxadiazole moiety.
      Adimule VM.
      Asian J Biomed Pharmaceut Sci. 4(37):1-5 (2014)

    • Physico-chemical characterization, cytotoxic activity, and biocompatibility studies of tamoxifen-loaded solid lipid nanoparticles prepared via a temperature-modulated solidification method.
      Lakkadwala S, Nguyen S, Lawrence J, Nauli SM, Nesamony J.
      Journal of microencapsulation. 31(6):590-9 (2014)

    • Development of a new rapid isolation device for circulating tumor cells (CTCs) using 3D palladium filter and its application for genetic analysis.
      Yusa A, Toneri M, Masuda T, Ito S, Yamamoto S, Okochi M, Kondo N, Iwata H, Yatabe Y, Ichinosawa Y, Kinuta S, Kondo E, Honda H, Arai F, Nakanishi H.
      Public Library of Science one. 9(2):e88821 (2014)

    • Fabrication of nanohydroxyapatite/poly(caprolactone) composite microfibers using electrospinning technique for tissue engineering applications.
      Hassan MI, Sun T, Sultana N.
      J Nanomaterials 2014:209049 (2014)

    • Real-time infrared thermography detection of magnetic nanoparticle hyperthermia in a murine model under a non-uniform field configuration.
      Rodrigues HF, Mello FM, Branguinho LC, Zufelato N, Silverira-Lacerda EP, Bakuzis AF.
      International journal of hyperthermia. 29(8):752-67 (2013)

    • Isolation of living circulating tumor cells (CTCs) from peripheral blood using size-based device and its application to CTC technology in mice. IEEE 2013 International Symposium on Micro-NanoMechatronics and Human Science (MHS).
      Yusa A, Matsumoto Y, Terazawa K, Masuda T, Arai F, Nakanishi H. MHS2013.
      DOI: 10.1109/MHS.2013.6710455 (2013)

    • Anti-apoptotic effect of clusterin on cisplatin-induced cell death of retinoblastoma cells.
      Song HB, Jun HO, Kim JH, Yu YS, Kim KW, Min BH, Kim JH.
      Oncology Reports. 30(6):2713-8 (2013)

    • Intracellular glutathione pools are heterogeneously concentrated.
      Montero D, Tachibana C, Winther JR, Appenzeller-Herzog C.
      Redox Biology. 1(1):508-13 (2013)

    • The interaction between C35 and ΔNp73 promotes chemo-resistance in ovarian cancer cells.
      Leung TH, Wong SC, Chan KK, Chan DW, Cheung AN, Ngan HY.
      British Journal of Cancer. 109(4):965-75 (2013)

    • Drug release from porous silicon for stable neural interface.
      Sun T, Tsang WM, Park WT.
      Appl Surface Sci. 292:843-851 (2013)

    • Attenuated mitochondrial NADP+-dependent isocitrate dehydrogenase activity induces apoptosis and hypertrophy of H9c2 cardiomyocytes.
      Lee JH, Park JW.
      Biochimie. 99:110-8. (2013)

    • Targeting mutant p53 by a SIRT1 activator YK-3-237 inhibits the proliferation of triple-negative breast cancer cells.
      Yi YW, Kang HJ, Kim HJ, Kong Y, Brown ML, Bae I.
      Oncotarget. 4(7):984-94 (2013)

    • Inhibition of checkpoint kinase 2 (CHK2) enhances sensitivity of pancreatic adenocarcinoma cells to gemcitabine.
      Duong HQ, Hong YB, Kim JS, Lee HS, Yi YW, Kim YJ, Wang A, Zhao W, Cho CH, Seong YS, Bae I.
      Journal of cellular and molecular medicine. 17(10):1261-70 (2013)

    • Characterization of enterovirus 71 replication kinetics in human colorectal cell line, HT29.
      Liu YLE, Timms P, Hafner LM, Tan TL, Tan KH, Tan EL.
      SpringerPlus. 2(1):267 (2013)

    • Inhibition of the PI3K/AKT pathway potentiates cytotoxicity of inhibitors for EGFR kinase in triple-negative breast cancer cells.
      Yi YW, Hong W, Kang HJ, Kim HJ, Zhao W, Wang A, Seong YS, Bae I.
      Journal of cellular and molecular medicine. 17(5):648-56 (2013)

    • Inhibition of NRF2 by PIK-75 augments sensitivity of pancreatic cancer cells to gemcitabine.
      Duong HQ, Yi YW, Kang HJ, Hong YB, Tang W, Wang A, Seong YS, Bae I.
      International journal of oncology. 44(3):959-69 (2013)

    • The effects of non-thermal atmospheric pressure plasma jet on attachment of osteoblast.
      Kwon JS, Kim YH, Choi EH, Kim KN.
      Current Applied Physics 13:S42-S47. (2013)

    • Towards an optimal and unbiased approach for tumor cell isolation.
      Sajay BNG, Chan CP, Ahmad H, Chung WC, Puiu PD, Rahman ARA.
      Biomedical Microdevices. 15(4):699-709 (2013)

    • BML-275, an AMPK inhibitor, induces DNA damage, G2/M arrest and apoptosis in human pancreatic cancer cells.
      Duong HQ, Hwang JS, Kim HJ, Seong YS, Bae I.
      International Journal of Oncology. 41(6):2227-36 (2012)

    • Aldehyde dehydrogenase 1A1 confers intrinsic and acquired resistance to gemcitabine in human pancreatic adenocarcinoma MIA PaCa-2 cells.
      Duoung HQ, Hwang JS, Kim HJ, Kang HJ, Seong YS, Bae I.
      International journal of oncology. 41(3):855-61 (2012)

    • FAS1 domain protein inhibits VEGF165-induced angiogenesis by targeting the interaction between VEGFR-2 and αvβ3 integrin.
      Nam JO, Son HN, Jun E, Cha K, Lee BH, Park RW, Kim IS.
      Molecular Cancer Research. 10(8):1010-20 (2012)

    • BRCA1 negatively regulates IGF-1 expression through an estrogen-responsive element-like site.
      Kang HJ, Yi YW, Kim HJ, Hong YB, Seong YS, Bae I.
      Cell Death & Disease. 3:e336.

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      LUNA™

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      How to Count Cells An Overview of Cell Counting Methods

      Product literature

      2018-04-13 | 720.79 KB
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      Application of a non-hazardous vital dye for cell counting with automated cell counters

      Product literature

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        Autoexposure mode update

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        2018-04-16 | 2.2 MB
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        LUNA™ software ver. 3.4.0

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        Cell DB
          • LogosBiosystems LUNA AutomatedCellCounter 293A
          • 293A

            Experimental condition

            Healthy 293A cells (a subclone of 293 cell line) from routine cell culture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5 | Roundness: 60 % | Min. cell size: 7 um
            Max. cell size: 60 um | Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter 293T
          • 293T

            Experimental condition

            293T cells (a subclone of 293 cell line) from routine cell culture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 7 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter BT-549
          • BT-549

            Experimental condition

            Healthy BT-549 cells (breast carcinoma) from a routine subculture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter CC-503 Chlamydomonas
          • CC-503 (Chlamydomonas)

            Experimental condition

            Micro-algae CC-503 strain (Chlamydomonas) was stained with trypan blue and counted with the LUNA automated cell counter. Althougth algae cells were identified as dead cells with LUNA, total number of cell was accurately counted.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 3 um
            Max. cell size: 12 um
            Enhanced declustering: On


            Kindly provided by [Biomedical Engineering Lab., Seoul National University]

          • LogosBiosystems LUNA AutomatedCellCounter CHO bioreactor culture
          • CHO (bioreactor culture)

            Experimental condition

            Suspension CHO cells from a bioreactor culture were counted with the LUNA automated cell counter.

            Cell counting result

            Total cell concentration: 8.6 x 10E6 cells/ml
            Live cell concentration: 8.4 x 10E6 cells/ml
            Dead cell concentration: 2.7 x 10E5 cells/ml
            Viabillity: 96.9 %
            Average cell size: 12.5 um

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter COS-7
          • COS-7

            Experimental condition

            COS-7 cells from routine cell culture were counted with the LUNA automated cell counter.

            result

            Total cell concentration: 6.0 x 10E5 cells/ml
            Live cell concentration: 5.7 x 10E5 cells/ml
            Dead cell concentration: 3.0 x 10E4 cells/ml
            Viabillity: 95.1 %
            Average cell size: 22.8 um

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • LogosBiosystems LUNA AutomatedCellCounter DAUDI
          • DAUDI

            Experimental condition

            DAUDI cells from routine cell culture were counted with the LUNA automated cell counter.

            result

            Total cell concentration: 1.9 x 10E6 cells/ml
            Live cell concentration: 1.7 x 10E6 cells/ml
            Dead cell concentration: 1.8 x 10E5 cells/ml
            Viabillity: 90.8 %
            Average cell size: 9.2 um

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • GT1-7

            Experimental condition

            Mouse hypothalamic neuron GT1-7 cells were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 7
            Roundness: 60 %
            Min. cell size: 10 um
            Max. cell size: 30 um
            Enhanced declustering: On

            Kindly provided by [Neuroendocrine Lab., Seoul National University]

          • HeLa

            Experimental condition

            HeLa cells were trypsinized at a mild condition and counted with the LUNA automated cell counter. LUNA showed an excellent performance to count highly clustered HeLa cells.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter HL-60 100viability
          • HL-60 (100% viability)

            Experimental condition

            Healthy HL-60 cells from routine cell culture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • LogosBiosystems LUNA AutomatedCellCounter HL-60 50viability
          • HL-60 (50 % viability)

            Experimental condition

            HL-60 cells were artificially killed by heating and mixed with healty cells (50:50). Mixed cells were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering : off

          • LogosBiosystems LUNA AutomatedCellCounter HL-60 highconcentration
          • HL-60 (high concentration)

            Experimental condition

            A high density HL-60 cells were prepared by standard centrifugation and counted with the LUNA automated cell counter. The concentration and the viability of this sample was calcuated as 1 X 10E7 cells/ml and 88.2 % each with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • LogosBiosystems LUNA AutomatedCellCounter HL-60 lowviability
          • HL-60 (low viability)

            Experimental condition

            HL-60 cells were artificially killed by heating and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um

          • LogosBiosystems LUNA AutomatedCellCounter Hs-578-T
          • Hs-578-T

            Experimental condition

            HS578-T cells from a routine cell culture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • LogosBiosystems LUNA AutomatedCellCounter IMR-32
          • IMR-32

            Experimental condition

            Human neuroblast IMR-32 cells were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 3
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

            Kindly provided from [Neuroendocrine Lab., Seoul National University]

          • LogosBiosystems LUNA AutomatedCellCounter Jurkat
          • Jurkat

            Experimental condition

            Jurkat cells were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter MCF10A
          • MCF 10A

            Experimental condition

            Healthy MCF 10A cells (a non-tumorigenic epithelial cell line) from a routine subculture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter MCF7
          • MCF7

            Experimental condition

            Healthy MCF7 cells (mammary gland; breast carcinoma) from a routine subculture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter MDA-MB-231
          • MDA-MB-231

            Experimental condition

            MDA-MB-231 cells from a routine subculture were counted with the LUNA automated cell counter

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • LogosBiosystems LUNA AutomatedCellCounter MDA-MB-436
          • MDA-MB-436

            Experimental condition

            MDA-MB-436 cells from a routine subculture were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • MDA-MB-468

            Experimental condition

            MDA-MB-468 cells from a routine subculture were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: Off

          • LogosBiosystems LUNA AutomatedCellCounter MIAPaCa-2
          • MIA PaCa-2

            Experimental condition

            Healthy MIA PaCa-2 cells (pancreas carcinoma) from a routine subculture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter N4 mHypoE-N4
          • N4 (mHypoE-N4)

            Experimental condition

            Embryonic mouse hypothalamus cell line N4 (mHypoE-N4) cells were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 7
            Roundness: 60 %
            Min. cell size: 10 um
            Max. cell size: 30 um
            Enhanced declustering: On

            Kindly provided by [Neuroendocrine Lab., Seoul National University]

          • N44 (mHypoE-N44)

            Experimental condition

            Embryonic mouse hypothalamus cell line N44 (mHypoE-N44) was stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 10 um
            Max. cell size: 60 um
            Enhanced declustering: On

            Kindly provided from [Neuroendocrine Lab., Seoul National University]

          • LogosBiosystems LUNA AutomatedCellCounter N7 mHypoE-N7
          • N7 (mHypoE-N7)

            Experimental condition

            Embryonic mouse hypothalamus cell line (mHypoE-N7) was stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 10 um
            Max. cell size: 60 um
            Enhanced declustering: On

            Kindly provided from [Neuroendocrine Lab., Seoul National University]

          • LogosBiosystems LUNA AutomatedCellCounter NIH3T3
          • NIH3T3

            Experimental condition

            Healthy NIH3T3 cells from a routine cell culture were counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 7 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter SCN2.2
          • SCN 2.2

            Experimental condition

            Immortalized suprachiasmatic nucleus (SCN 2.2) cells were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 10 um
            Max. cell size: 60 um
            Enhanced declustering: On

            Kindly provided from [Neuroendocrine Lab., Seoul National University]

          • LogosBiosystems LUNA AutomatedCellCounter SUM149PT
          • SUM149PT

            Experimental condition

            SUM149PT cells from a routine subculture were stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • U2OS

            Experimental condition

            Human osteosarcoma U2OS cells from a routine subculture were stained with trypan blue and counted with the LUNA automated cell counter

            Cell counting protocol

            Noise reduction: 5
            Roundness: 70 %
            Min. cell size: 10 um
            Max. cell size: 60 um
            Enhanced declustering: On

          • LogosBiosystems LUNA AutomatedCellCounter UWB1.289
          • UWB1.289

            Experimental condition

            Human ovarian cancer cell UWB1.289 was stained with trypan blue and counted with the LUNA automated cell counter.

            Cell counting protocol

            Noise reduction: 5
            Roundness: 60 %
            Min. cell size: 5 um
            Max. cell size: 60 um
            Enhanced declustering: On

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        ORDERING INFORMATION

        Cat # Product Qty
        L10001 LUNA™ Automated Cell Counter 1 unit
        L12001 LUNA™ Cell Counting Slides, 50 Slides 1 box
        L12002 LUNA™ Cell Counting Slides, 500 Slides 10 boxes
        L12003 LUNA™ Cell Counting Slides, 1000 Slides 20 boxes
        L12011 LUNA™ Reusable Slide 1 unit
        T13001 Trypan Blue Stain, 0.4% 2 x 1 mL
        L13002 Erythrosin B Stain 2 x 1 mL
        B13101 LUNA™ Standard Beads 2 x 1 mL
        L14003 LUNA™ IQ/OQ Protocol 1 copy
        P10001 LUNA™ Printer 1 unit
        P12001 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls