• Fluorescence Cell Counter

    LUNA-STEM™ Automated Fluorescence Cell Counter for Stem Cells & SVF

    The LUNA-STEM™ takes adipose-derived stem cell and SVF samples to count live nucleated cells, dead nucleated cells, and non-nucleated cells with precision and consistency for downstream procedures.
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When using tissue-derived cells such as stem cells or the cells from the adipose stromal vascular fraction (SVF), knowing cell concentration and viability is crucial to ensure and control the quality of downstream procedures. Tissue-derived samples are often contaminated with red blood cells and noncellular debris, making cell counting a time-consuming, subjective, and error-prone process. The LUNA-STEM™ is equipped with dual fluorescence and brightfield optics to distinguish live and dead nucleated cells from non-nucleated cells. Samples are stained with nucleic acid dyes to determine the concentration and viability of mononucleated cells. Non-nucleated cells are distinguished from debris and also counted. In as little as 30 seconds, the LUNA-STEM™ reports total cell concentration, nucleated cell concentration, non-nucleated cell concentration, cell viability, and average cell size. A high-resolution image with live cells labeled with green circles and dead cells with red circles can be viewed to confirm data visually.

WHAT THE LUNA-STEM™ DOES BEST

  • COUNT TISSUE-DERIVED CELLS

    Using dual fluorescence and brightfield optics, the LUNA-STEM™ can distinguish between cells and debris.

  • 30 SECONDS TO RESULTS

    In as little as 30 seconds, the LUNA-STEM™ captures, merges, and analyzes images from three channels to produce cell count and viability data.

  • NUCLEATED AND NON-NUCLEATED CELL ANALYSIS

    Nucleic stains make sure that nucleated cells are counted as live or dead cells whereas nonnucleated cells are counted as non-nucleated cells.

  • EASY RECORD KEEPING

    PDF reports complete with images and data are automatically generated and can be transferred to a PC or printed for your records.

THE SOFTWARE THAT CHANGES EVERYTHING.

VERIFY RESULTS IMMEDIATELY USE THE DILUTION CALCULATOR CUSTOMIZE & SAVE PROTOCOLS

THE LOWEST COST. THE GREATEST CONVENIENCE.

Perfect for labs on a budget, our LUNA™ family of automated cell counters work with the LUNA™ Reusable Slide. The LUNA Reusable Slide delivers the affordability of manual cell counting without the associated time, subjectivity, and user-to-user variability. Our disposable slides offer the ultimate counting experience. With no mess or cleanup, these precision slides are convenient and maintain the highest standard of cell counting accuracy.
Compatible Reagents
    Cat # Product Qty MSDS Product Insert
    F23001 Acridine Orange/Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23001] Acridine Orange Propidium Iodide (AOPI) Stain.pdfDownload 
    F23001 Acridine Orange,Propidium Iodide Stain VL1801-01.pdfDownload 
    F23002 Acridine Orange Stain

    2 x 0.5 mL

    FileAction
    [F23002] Acridine Orange (AO) Stain.pdfDownload 
    F23002 Acridine Orange Stain VL1801-01.pdfDownload 
    F23003 Propidium Iodide Stain

    2 x 0.5 mL

    FileAction
    [F23003] Propidium Iodide (PI) Stain.pdfDownload 
    F23003 Propidium Iodide Stain VL1801-01.pdfDownload 
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    WHAT DO OUR CUSTOMERS HAVE TO SAY?

    Testimonials
    • Patrick Mallory, DO

      Mallory Family Wellness

      The LUNA-STEM unit has proven to be a very convenient and useful addition to our stem cell procedures. It provides a quicker and more accurate method of counting nucleated cells from both bone marrow and adipose tissue than the traditional manual method. The printed reports are clean and easy to import to the patient’s electronic medical record. The process is also not only quicker than manual cell counting but is also cleaner, avoiding the mess, hassle and expense of dyes and hemocytometer slides and covers. The training provided by the LUNA-STEM field scientist was thorough, allowing quick implementation of the process. I highly recommend this unit without reservation to any clinic or facility that provides stem cell treatments.

    • Ann Erickson, Senior Research Associate

      California Pacific Medical Center Research Institute

      After 25 years of being a proponent of manual counting I now find myself a LUNA-STEM convert.

    • Roger Hogue, MD

      Hogue Cosmetic Surgery

      As a Regenerative Medicine Specialist with experience in the isolation and deployment of stromal vascular fraction (SVF) containing adipocyte-derived stem cells (ADSCs) for the treatment of degenerative orthopedic conditions since 2011, I have been asked to share my thoughts about the LUNA-STEM that I use in my practice.
      During the initial years, I used a manual microscopy method using trypan blue staining to perform cell counts and viability assessments of isolated SVF specimens. During the past year, I purchased a LUNA-STEM that uses AO/PI staining. The LUNA-STEM has increased my time efficiency and accuracy in determining cell counts for nucleated and non-nucleated cells within the SVF specimen, as well as cell viability assessments. The interactive touch screen on the LUNA-STEM is easy to use.
      Manual image focusing on an SVF specimen placed on a counting slide, referred to as a PhotonSlide, is very straightforward. Data reports and captured images can quickly be saved as a PDF file on an inserted USB thumb drive. For chart documentation, the LUNA Printer is great for an immediate printout of counting results.
      I can honestly say that I will not be going back to my old trypan blue staining method. The LUNA-STEM universally does a better job at cell counting and viability than I can do.

    • Heather Main, PhD

      Genea Biocells

      We have two LUNA-STEM systems. We like it because it’s a plug and go, no startup or shut down maintenance and only a single step for fluorescence based cell count and viability analysis. For what it is, the price competitiveness of the LUNA-STEM system and consumables cannot be beaten.

    • Andrew C. Wesely, MD

      Sierra Stem Cell Institute

      At Sierra Stem Cell Institute, we provide regenerative injection treatments for orthopedic and other musculoskeletal conditions, utilizing PRP and stem cell preparations from bone marrow and lipoaspirate. Knowing the cell count, and viability of our preparations, at the point of care became very important. The measuring process also needed to be easy implement, simple to perform, and cost effective. The LUNA-STEM proved an excellent solution to our needs and performed beyond expectations. I highly recommend this technology to any clinic providing these services.

    + SHOW OUR CUSTOMERS

    WHERE THE LUNA-STEM™ HAS BEEN CITED

    Citations
    • Procházka V, Jurčíková J, Laššák O, Vítková K, Pavliska L, Porubová L, Buszman PP, Krauze A, Fernandez C, Jalůvka F, Špačková I, Lochman I, Jana D, Merfeld-Clauss S, March KL, Traktuev DO, Johnstone BH. Therapeutic potential of adipose-derived therapeutic factor concentrate for treating critical limb ischemia. Cell Transplant 2015; doi: 10.3727/096368915X689767.

    Resources

    Downloads
    • Icon
      LUNA-STEM brochure VL1605-01

      Brochure

      2018-04-26 | 1.25
    • Icon
      Logos Biosystems Products

      Brochure

      2018-04-15 | 193.4 MB
      • Icon
        LUNA-STEM™ software ver. 1.4.0

        Software

        2018-04-16 | 2.5 MB

        ORDERING INFORMATION

        Cat # Product Qty
        L30001 LUNA-STEM™ Automated Fluorescence Cell Counter 1 unit
        L12005 PhotonSlide™, 50 Slides 1 box
        L12006 PhotonSlide™, 500 Slides 10 boxes
        L12007 PhotonSlide™, 1000 Slides 20 boxes
        F23001 Acridine Orange/Propidium Iodide Stain 2 x 0.5 mL
        F23002 Acridine Orange Stain 2 x 0.5 mL
        F23003 Propidium Iodide Stain 2 x 0.5 mL
        F23212 Cell Dilution Buffer 5 x 20 mL
        F23102 LUNA™ Fluorescence Calibration Beads 1 x 0.5 mL
        P10001 LUNA™ Printer 1 unit
        P10002 LUNA™ Printer Paper - thermal, 700 prints 3 x 2 rolls